Evidence for nerve growth factor-mediated paracrine effects in human epidermis

Nerve growth factor (NGF) is critical to the development and maintenance of the peripheral nervous system, but its possible roles in other organ systems are less well characterized. We have recently shown that human epidermal melanocytes, pigment cells derived from the neural crest, express the NGF receptor (p75 NGF-R) in vitro (Peacocke, M., M. Yaar, C. P. Mansur, M. V. Chao, and B. A. Gilchrest. 1988. Proc. Natl. Acad. Sci. USA. 85:5282-5286). Using cultured human skin-derived cells we now demonstrate that the melanocyte p75 NGF-R is functional, in that NGF stimulation modulates melanocyte gene expression; that exposure to an NGF gradient is chemotactic for melanocytes and enhances their dendricity; and that keratinocytes, the dominant epidermal cell type, express NGF messenger RNA and hence are a possible local source of NGF for epidermal melanocytes in the skin. These combined data suggest a paracrine role for NGF in human epidermis.

• Publication year

  1991

• Venue

  Journal of Cell Biology

• Publication date

  1991-11-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1083/JCB.115.3.821PMID 1655813PMCID 2289176
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  1991cited by this paper
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  1991cited by this paper
• The trk proto-oncogene encodes a receptor for nerve growth factor.

  1991cited by this paper
• Expression and modulation of nerve growth factor in murine keratinocytes (PAM 212).

  1990cited by this paper
• Mechanisms of cutaneous inflammation. Interactions between epidermal cytokines, adhesion molecules, and leukocytes.

  1989cited by this paper
• Nerve growth factor induces growth and differentiation of human B lymphocytes.

  1989cited by this paper
• Estrogen regulation of epidermal growth factor receptor messenger ribonucleic acid.

  1988cited by this paper
• Epidermal growth factor (EGF) and hormones stimulate phosphoinositide hydrolysis and increase EGF receptor protein synthesis and mRNA levels in rat liver epithelial cells. Evidence for protein kinase C-dependent and -independent pathways.

  1988cited by this paper
• Wound macrophages express TGF-alpha and other growth factors in vivo: analysis by mRNA phenotyping.

  1988cited by this paper
• Oncogenes and growth factors.

  1988cited by this paper
• Nerve growth factor promotes human hemopoietic colony growth and differentiation.

  1988cited by this paper
• Induction of nerve growth factor receptors on cultured human melanocytes.

  1988cited by this paper
• Oncogenes and growth factors.

  1988cited by this paper
• The synthesis of nerve growth factor (NGF) in developing skin is independent of innervation.

  1988cited by this paper
• Isolation and sequence of a cDNA clone for human tyrosinase that maps at the mouse c-albino locus.

  1987cited by this paper
• Effects of epidermal growth factor and platelet-derived growth factor on c-fos and c-myc mRNA levels in normal human fibroblasts.

  1987cited by this paper
• Timing and site of nerve growth factor synthesis in developing skin in relation to innervation and expression of the receptor

  1987cited by this paper
• The nerve growth factor 35 years later.

  1987cited by this paper
• Changes of nerve growth factor synthesis in nonneuronal cells in response to sciatic nerve transection

  1987cited by this paper
• Some characteristics of histamine secretion from rat peritoneal mast cells stimulated with nerve growth factor.

  1986cited by this paper
• Gene transfer and molecular cloning of the human NGF receptor.

  1986cited by this paper
• Expression and structure of the human NGF receptor.

  1986cited by this paper
• Nerve growth factor activates Thy‐1 and neurofilament gene transcription in rat PC12 cells

  1986cited by this paper
• Nerve growth factor and epidermal growth factor induce rapid transient changes in proto-oncogene transcription in PC12 cells.

  1985influential reference
• Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: genomic complexity and molecular evolution of the gene.

  1985cited by this paper
• Substrate influences human epidermal melanocyte attachment and spreading in vitro.

  1985cited by this paper
• Nerve Growth Factors and Molecules of the Extracellular Matrix in Neuronal Development

  1985cited by this paper
• Neurotrophic factors.

  1985cited by this paper
• Selective cultivation of human melanocytes from newborn and adult epidermis.

  1984cited by this paper
• c-fos protein can induce cellular transformation: a novel mechanism of activation of a cellular oncogene.

  1984cited by this paper
• Immunohistochemical localization of endogenous nerve growth factor

  1984cited by this paper
• Human beta-nerve growth factor gene sequence highly homologous to that of mouse.

  1983cited by this paper
• Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed

  1983cited by this paper
• Homogeneously staining chromosomal regions contain amplified copies of an abundantly expressed cellular oncogene (c-myc) in malignant neuroendocrine cells from a human colon carcinoma.

  1983cited by this paper
• An endocrine approach to the control of epidermal growth: serum-free cultivation of human keratinocytes.

  1981cited by this paper
• Detection of pemphigoid antigen, pemphigus antigen, and keratin filaments by indirect immunofluorescence in cultured human epidermal cells.

  1980cited by this paper
• Growth of human keratinocytes on fibronectin -coated plates.

  1980cited by this paper
• Effects of aging and chronic sun exposure on melanocytes in human skin.

  1979cited by this paper
• Relationship between actinic damage and chronologic aging in keratinocyte cultures of human skin.

  1979cited by this paper
• Neuronal chemotaxis: chick dorsal-root axons turn toward high concentrations of nerve growth factor.

  1979cited by this paper
• Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.

  1979influential reference
• Nerve growth factor receptors. Characterization of two distinct classes of binding sites on chick embryo sensory ganglia cells.

  1979cited by this paper
• Selective enzyme induction in a nerve growth factor-responsive pheochromocytoma cell line (PC 12).

  1978cited by this paper
• DNA sequencing with chain-terminating inhibitors.

  1977cited by this paper
• The nerve growth factor: its role in growth, differentiation and function of the sympathetic adrenergic neuron.

  1976cited by this paper
• Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor.

  1976cited by this paper
• Nerve growth factor.

  1975cited by this paper
• Ribonucleic acid isolated by cesium chloride centrifugation.

  1974cited by this paper
• Adsorption of nerve growth factor onto surfaces. Implications for the assay in tissue culture.

  1973cited by this paper
• Nerve growth factor.

  1968cited by this paper
• Subunit structure of a high-molecular-weight form of the nerve growth factor from mouse submaxillary gland.

  1967cited by this paper
• A NERVE GROWTH-STIMULATING FACTOR ISOLATED FROM SARCOM AS 37 AND 180.

  1954cited by this paper

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  2023cites this paper
• Compartmentalized primary cultures of dorsal root ganglion neurons to model peripheral pathophysiological conditions

  2023cites this paper
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  2023cites this paper
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  2022cites this paper
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  2022cites this paper
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  2021cites this paper
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  2021cites this paper
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  2020cites this paper
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  2020cites this paper
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  2019cites this paper
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  2018cites this paper
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  2018cites this paper
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  2018cites this paper
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  2011cites this paper
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  2011cites this paper
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  2011influential citation
• Neurotrophins in healthy and diseased skin

  2011cites this paper
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• Nerve growth factor: the dark side of the icon.

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• DNA Polymorphisms: What They Are and Their Role in Human Pigmentation

  2008cites this paper
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  2008cites this paper
• Nerve growth factor beta/pro-nerve growth factor and their receptors in normal human oral mucosa.

  2007cites this paper
• Human skin pigmentation: melanocytes modulate skin color in response to stress

  2007cites this paper
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  2007cites this paper
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  2006cites this paper
• Basic fibroblast growth factor promotes melanocyte migration via increased expression of p125(FAK) on melanocytes.

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  2006cites this paper
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  2006cites this paper
• The reinnervation pattern of wounds and scars may explain their sensory symptoms.

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  2005cites this paper
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  2005cites this paper
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  2005cites this paper
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  2005cites this paper
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  2005cites this paper
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  2005cites this paper
• Nerve growth factor modulate proliferation of cultured rabbit corneal endothelial cells and epithelial cells.

  2005cites this paper
• Melanocyte biology: before, during, and after the Fitzpatrick era.

  2004cites this paper
• Nerve growth factor receptors and signaling in breast cancer.

  2004cites this paper
• Hepatocyte growth factor controls the proliferation of cultured epidermal melanoblasts and melanocytes from newborn mice.

  2004cites this paper
• Estradiol induces proliferation of keratinocytes via receptor‐ mediated mechanisms

  2004cites this paper
• Neurotrophic factors and their receptors in human sensory neuropathies.

  2004cites this paper
• Epithelial growth control by neurotrophins: leads and lessons from the hair follicle.

  2004cites this paper
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