Apparent turnover of mitochondrial deoxyribonucleic acid and mitochondrial phospholipids in the tissues of the rat.

Abstract The apparent turnovers of mitochondrial DNA and of the phospholipids, cardiolipin, lecithin, and phosphatidyl ethanolamine, have been determined simultaneously in the mitochondria of rat liver, kidney, brain, and heart. The turnover rate of mitochondrial DNA in liver was similar to previously reported turnover rates of other mitochondrial components. Mitochondrial DNA has a half-life of 6.7 days in heart, 9.4 days in liver, 10.4 days in kidney, and about 31 days in brain. The turnover of mitochondrial phospholipids is complex: in the liver and kidney their turnover rates are similar to that of the mitochondrial DNA. In the other organs studied slower and nonexponential turnover rates were found. There was no apparent turnover of nuclear DNA in liver, kidney, or brain during the experiment. A relatively rapid rate of turnover with a half-life of about 30 days was found for heart nuclear DNA.

• Publication year

  1969

• Venue

  Journal of Biological Chemistry

• Publication date

  1969-03-25

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/S0021-9258(18)91795-3PMID 5773057
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Synthesis of new strands of mitochondrial and nuclear deoxyribonucleic acid by semiconservative replication.

  1969cited by this paper
• Exchange of phospholipids between liver mitochondria and microsomes in vitro.

  1968cited by this paper
• The phospholipids of various sheep organs, rat liver and of their subcellular fractions.

  1968cited by this paper
• Extranuclear DNA.

  1968cited by this paper
• Biochemical aspects of the biogenesis of mitochondria

  1968cited by this paper
• Thymidine content and turnover in the rat.

  1968cited by this paper
• The metabolism of mitochondrial proteins. 3. The dynamic state of rat liver mitochondria.

  1968cited by this paper
• Ultracentrifuge and electron microscope studies on the structure of mitochondrial DNA.

  1967cited by this paper
• Separation of acidic phospholipids by one-dimensional thin-layer chromatography.

  1967cited by this paper
• Biosynthesis of DNA by Isolated Mitochondria: Incorporation of Thymidine Triphosphate-2- C14

  1967cited by this paper
• Characterization of the ribonucleic acid synthesized in an isolated nuclear system from rat heart muscle.

  1967cited by this paper
• The turnover of the protein components of mitochondria from rat liver, kidney, and brain.

  1967cited by this paper
• Incorporation of 33Pi into mitochondrial and nuclear DNA in regenerating liver.

  1967cited by this paper
• Mitochondrial structure and compartmentation

  1967cited by this paper
• Mitochondrial transfer ribonucleic acids.

  1967cited by this paper
• Ribosomal RNA and ribosomes from mitochondria of Neurospora crassa.

  1967cited by this paper
• Turnover of mitochondrial components of normal and essential fatty acid-deficient rats.

  1967cited by this paper
• Turnover of constituents of the endoplasmic reticulum membranes of rat hepatocytes.

  1967cited by this paper
• Purification of lipids from nonlipid contaminants on Sephadex bead columns.

  1966cited by this paper
• Circular mitochondrial DNA.

  1966cited by this paper
• Turnover of brain mitochondrial membrane lipids.

  1966cited by this paper
• Delta-aminolevulinic acid synthetase. II. Induction in rat liver.

  1966cited by this paper
• Circular DNA filaments from mouse mitochondria.

  1966cited by this paper
• The circularity of mitochondrial DNA.

  1966cited by this paper
• The structural gene for yeast cytochrome C.

  1966cited by this paper
• [A means of differentiation of the mitochondrial membrane, evoking the bacterial mesosome].

  1966cited by this paper
• Density-Gradient centrifugation of DNA in a fixed-angle rotor: A higher order of resolution

  1966cited by this paper
• BEHAVIOR OF PLASMA LIPOPROTEINS DURING EXCHANGE OF PHOSPHOLIPIDS BETWEEN PLASMA AND ERYTHROCYTES.

  1965cited by this paper
• BEHAVIOR OF PLASMA LIPOPROTEINS DURING EXCHANGE OF PHOSPHOLIPIDS BETWEEN PLASMA AND ERYTHROCYTES.

  1965cited by this paper
• NUCLEIC ACIDS OF CHLOROPLASTS AND MITOCHONDRIA IN SWISS CHARD

  1965cited by this paper
• DEOXYRIBONUCLEIC ACID IN ISOLATED RAT-LIVER MITOCHONDRIA.

  1965cited by this paper
• Isolation of deoxyribonucleic acid from mitochondria of chick embryo heart and liver.

  1965cited by this paper
• Distribution of lipids in subcellular particles of guinea-pig brain.

  1964cited by this paper
• DNA IN MITOCHONDRIA OF NEUROSPORA CRASSA.

  1964cited by this paper
• FOCAL DEGRADATION AS A BIOLOGICAL PROCESS.

  1964cited by this paper
• Quantitative analysis of phospholipids by thin-layer chromatography.

  1964cited by this paper
• A procedure for the isolation of enzymically active rat-liver nuclei.

  1964cited by this paper
• Lipid composition of beef brain, beef liver, and the sea anemone: Two approaches to quantitative fractionation of complex lipid mixtures

  1963cited by this paper
• The lipid composition of rat-liver mitochondria, fluffy layer and microsomes.

  1962cited by this paper
• "Lifespan" of liver cells. Autoradio-graphic study using tritiated thymidine in normal, cirrhotic, and partially hepatectomized rats.

  1961cited by this paper
• Turnover of rat-liver mitochondria.

  1961cited by this paper
• The metabolic stability of rat liver deoxyribonucleic acid: a turnover study.

  1960cited by this paper
• The phospholipids of the erythrocyte 'ghosts' of various species.

  1960cited by this paper
• A fractionating column for analysis of nucleic acids.

  1960cited by this paper
• Heterogeneity of Liver Lecithin isolated by Chromatography on Silicic Acid Columns

  1960cited by this paper
• Phosphorus assay in column chromatography.

  1959cited by this paper
• Incorporation of labeled amino acids into the protein of muscle and liver mitochondria.

  1958cited by this paper
• The enzymatic formation of lecithin from cytidine diphosphate choline and D-1,2-diglyceride.

  1958cited by this paper
• A simple method for the isolation and purification of total lipides from animal tissues.

  1957cited by this paper
• A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.

  1956cited by this paper
• A microspectrophotometric method for the determination of cytochrome oxidase.

  1951cited by this paper

• Mitochondrial DNA Deletion Mutations: A Molecular Cause of Age-Induced Skeletal Muscle Fiber Dysfunction and Fiber Death Contributing to Sarcopenia.

  2025cites this paper
• Selfish mutations promote age-associated erosion of mtDNA integrity in mammals

  2025cites this paper
• Turnover and Quality Control of Mitochondrial DNA

  2025cites this paper
• Prenatal ambient temperature exposure and cord blood and placental mitochondrial DNA content: Insights from the ENVIRONAGE birth cohort study.

  2025cites this paper
• An alternative model for maternal mtDNA inheritance

  2025cites this paper
• High tide or low tide: the transport and metabolism of mitochondrial nucleotides

  2025cites this paper
• Mitochondrial dysfunction, cause or consequence in neurodegenerative diseases?

  2024cites this paper
• Age-induced changes in skeletal muscle mitochondrial DNA synthesis, quantity, and quality in genetically unique rats

  2024influential citation
• Selection promotes age-dependent degeneration of the mitochondrial genome

  2024cites this paper
• Mitochondrial DNA Breaks Activate an Integrated Stress Response to Reestablish Homeostasis

  2023cites this paper
• Mitochondrial mechanisms in Alzheimer's disease: quest for therapeutics.

  2023cites this paper
• Mitochondrial heterogeneity in diseases

  2023cites this paper
• Key Amino Acid Residues of Mitochondrial Transcription Factor A Synergize with Abasic (AP) Site Dynamics To Facilitate AP-Lyase Reactions.

  2023cites this paper
• The prevalence of dementia in humans could be the result of a functional adaptation

  2023cites this paper
• The long term effects of uncoupling interventions as a therapy for dementia in humans

  2023cites this paper
• Modeling mitochondria, where are the numbers?

  2023cites this paper
• High-throughput single-cell analysis reveals progressive mitochondrial DNA mosaicism throughout life

  2023cites this paper
• Diabetic Encephalopathy: Role of Oxidative and Nitrosative Factors in Type 2 Diabetes

  2022cites this paper
• A comparison of mtDNA deletion mutant proliferation mechanisms

  2022cites this paper
• mtDNA breaks compromise mitochondrial membrane ultrastructure and trigger an integrated stress response

  2022cites this paper
• Dynamic features of human mitochondrial DNA maintenance and transcription

  2022cites this paper
• Stochastic survival of the densest and mitochondrial DNA clonal expansion in aging

  2022cites this paper
• An explanation of how mutant and wild-type mitochondria might stably co-exist in inherited mitochondrial diseases

  2022cites this paper
• Estimating the allele frequency threshold of the pathogenic mtDNA variant m.3243A>G tolerated by human myofibres

  2022cites this paper
• In vivo mitochondrial base editing via adeno-associated viral delivery to mouse post-mitotic tissue

  2022cites this paper
• Design of a digital‐PCR assay to quantify fragmented human mitochondrial DNA

  2021influential citation
• Mitochondrial functional resilience after TFAM ablation in the adult heart.

  2021cites this paper
• Tissue specificity of energy metabolism in mitochondria

  2021cites this paper
• Regulation of Heme Oxygenase and Its Cross-Talks with Apoptosis and Autophagy under Different Conditions in Drosophila

  2021cites this paper
• Genetic modulators associated with regulatory surveillance of mitochondrial quality control, play a key role in regulating stress pathways and longevity in C. elegans.

  2021cites this paper
• The molecular pathology of pathogenic mitochondrial tRNA variants

  2021cites this paper
• The Effect of Mitochondrial DNA Half-Life on Deletion Mutation Proliferation in Long Lived Cells

  2021cites this paper
• Reduced Shmt2 expression impairs mitochondrial folate accumulation and respiration, and leads to uracil accumulation in mouse mitochondrial DNA

  2021cites this paper
• The TFAM-to-mtDNA ratio defines inner-cellular nucleoid populations with distinct activity levels.

  2021cites this paper
• Mitophagy in Alzheimer’s Disease and Other Age-Related Neurodegenerative Diseases

  2020cites this paper
• Survival of the densest accounts for the expansion of mitochondrial mutations in ageing

  2020cites this paper
• Mitochondrial DNA Damage: Prevalence, Biological Consequence and Emerging Pathways.

  2020cites this paper
• Mitochondrial dynamics and bioenergetics regulated by netrin‐1 in oligodendrocytes

  2020cites this paper
• Improvement in mitochondrial function underlies the effects of ANNAO tablets on attenuating cerebral ischemia-reperfusion injuries.

  2020cites this paper
• The significance of mitochondrial DNA half-life to the lifespan of post-mitotic cells

  2020cites this paper
• Extreme heterogeneity of human mitochondrial DNA from organelles to populations

  2020cites this paper
• Mitochondrial functional resilience after TFAM ablation in adult cardiomyocytes

  2020cites this paper
• Extreme heterogeneity of human mitochondrial DNA from organelles to populations

  2020cites this paper
• Slc25a36 modulates pluripotency of mouse embryonic stem cells by regulating mitochondrial function and glutathione level.

  2019cites this paper
• Mitochondrial transcription factor A promotes DNA strand cleavage at abasic sites

  2019cites this paper
• Mitochondrial Genome Maintenance: Damage and Repair Pathways

  2019cites this paper
• ALZHEIMER’S DISEASE: A CONSEQUENCE OF IMPAIRED MITOPHAGY?

  2019cites this paper
• Energetic costs of cellular and therapeutic control of stochastic mitochondrial DNA populations

  2019cites this paper
• Mitochondrial DNA degradation: A quality control measure for mitochondrial genome maintenance and stress response.

  2019cites this paper
• Resolving the Enigma of the Clonal Expansion of mtDNA Deletions

  2018cites this paper
• Loss of mtDNA activates astrocytes and leads to spongiotic encephalopathy

  2018cites this paper
• The Mitochondrial Endonuclease M20 Participates in the Down-Regulation of Mitochondrial DNA in Pollen Cells1

  2018cites this paper
• Preferential amplification of a human mitochondrial DNA deletion in vitro and in vivo

  2018cites this paper
• Chloroplasts and Mitochondria: Similarities and Differences

  2018cites this paper
• Oxidative Stress and the Central Nervous System

  2017cites this paper
• Mitochondrial DNA Mutagenesis in Metazoa: From Phenotypes to Rescue

  2017cites this paper
• Stochastic Models for Evolving Cellular Populations of Mitochondria: Disease, Development, and Ageing

  2017cites this paper
• Energetic costs of cellular and therapeutic control of stochastic mtDNA populations

  2017cites this paper
• Transcription profiling suggests that mitochondrial topoisomerase IB acts as a topological barrier and regulator of mitochondrial DNA transcription

  2017cites this paper
• A Mitochondrial Biomarker-Based Study of S-Equol in Alzheimer’s Disease Subjects: Results of a Single-Arm, Pilot Trial

  2017cites this paper
• Alterations in Mitochondrial Quality Control in Alzheimer’s Disease

  2016cites this paper
• Mechanics of mitochondrial motility in neurons.

  2016cites this paper
• DNA-polymerase guided elimination of paternal mitochondrial genomes: An escape-proof obstacle to their transmission

  2016cites this paper
• Single-cell analysis of mitochondrial DNA.

  2015cites this paper
• Mitochondrial DNA: A disposable genome?

  2015cites this paper
• Mitochondrial DNA Mutations in Ageing and Disease: Implications for HIV?

  2015cites this paper
• Flawed phospholipid formation or faulty fatty acid oxidation: Determining the cause of mitochondrial dysfunction in hearts lacking ACSL1

  2015cites this paper
• Oxydative Metabolism in Optic Nerve Myelin: New Perspectives in Hereditary Optic Neuropathies

  2015cites this paper
• Context-Dependent Role of Mitochondrial Fusion-Fission in Clonal Expansion of mtDNA Mutations

  2015cites this paper
• Mitochondrial dysfunction in aging: Much progress but many unresolved questions

  2015cites this paper
• using a stable isotope-mass spectrometric technique Measurement of mitochondrial DNA synthesis in vivo

  2015cites this paper
• Loss of long‐chain acyl‐CoA synthetase isoform 1 impairs cardiac autophagy and mitochondrial structure through mechanistic target of rapamycin complex 1 activation

  2015cites this paper
• Effect of one month duration ketogenic and non-ketogenic high fat diets on mouse brain bioenergetic infrastructure

  2015cites this paper
• Mechanisms of oxidative stress resistance in the brain: Lessons learned from hypoxia tolerant extremophilic vertebrates.

  2015cites this paper
• Effects of 5'-fluoro-2-deoxyuridine on mitochondrial biology in Caenorhabditis elegans.

  2014influential citation
• Mitochondrial biogenesis: a therapeutic target for neurodevelopmental disorders and neurodegenerative diseases.

  2014cites this paper
• Mitochondrial DNA disease and developmental implications for reproductive strategies

  2014cites this paper
• Mitochondrial mutations and ageing: can mitochondrial deletion mutants accumulate via a size based replication advantage?

  2014cites this paper
• MtDNA segregation in heteroplasmic tissues is common in vivo and modulated by haplotype differences and developmental stage.

  2014cites this paper
• Mitochondrial DNA mutations in aging.

  2014cites this paper
• Oxidative Stress Is Not a Major Contributor to Somatic Mitochondrial DNA Mutations

  2014cites this paper
• Transcription could be the key to the selection advantage of mitochondrial deletion mutants in aging

  2014cites this paper
• Mathematical models of mitochondrial aging and dynamics.

  2014cites this paper
• Inhalable particulate matter and mitochondrial DNA copy number in highly exposed individuals in Beijing, China: a repeated-measure study

  2013cites this paper
• Mitochondrial dynamics and quality control in ageing

  2013cites this paper
• Regulation of mitochondrial and nonmitochondrial protein turnover by the PINK1-Parkin pathway

  2013cites this paper
• Effect of Cholinergic Signaling on Neuronal Cell Bioenergetics

  2013cites this paper
• Mitochondrial mutations and aging: random drift is insufficient to explain the accumulation of mitochondrial deletion mutants in short‐lived animals

  2013cites this paper
• Mitochondrial Dynamics and Quality Control

  2013cites this paper
• Mathematical Modeling of the Role of Mitochondrial Fusion and Fission in Mitochondrial DNA Maintenance

  2013cites this paper
• Placental Mitochondrial DNA Content and Particulate Air Pollution during in Utero Life

  2012cites this paper
• Role of Mitochondrial Dynamics and Autophagy in Removal of Helix-Distorting Mitochondrial DNA Damage

  2012cites this paper
• Zidovudine Exposure in HIV-1 Infected Tanzanian Women Increases Mitochondrial DNA Levels in Placenta and Umbilical Cords

  2012cites this paper
• Crosstalk between mitochondrial (dys)function and mitochondrial abundance

  2012cites this paper
• Disease mechanisms in mitochondrial maintenance disorders

  2012cites this paper
• Mitochondrial transport in neurons: impact on synaptic homeostasis and neurodegeneration

  2012cites this paper
• Is mitochondrial DNA turnover slower than commonly assumed?

  2012influential citation
• Correlated Tissue Expression of Genes of Cytoplasmic and Mitochondrial Nucleotide Metabolisms in Normal Tissues is Disrupted in Transformed Tissues

  2012cites this paper
• Human Mitochondrial Mutations and Repair

  2011cites this paper
• Age-related changes in brain mitochondrial DNA deletion and oxidative stress are differentially modulated by dietary fat type and coenzyme Q₁₀.

  2011cites this paper