Repressor titration: a novel system for selection and stable maintenance of recombinant plasmids.

No abstract is available for this paper.

• Publication year

  1998

• Venue

  Nucleic Acids Research

• Publication date

  1998-05-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1093/NAR/26.9.2120PMID 9547269PMCID PMC147518
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• A temperature sensitive RecA protein of Escherichia coli

  2004cited by this paper
• Recombinase binding specificity at the chromosome dimer resolution site dif of Escherichia coli.

  1997cited by this paper
• The expression of bacterial nitroreductase in transgenic mice results in specific cell killing by the prodrug CB1954

  1997cited by this paper
• Immunostimulatory DNA Sequences Necessary for Effective Intradermal Gene Immunization

  1996cited by this paper
• Site‐specific recombination in the replication terminus region of Escherichia coli: functional replacement of dif.

  1995cited by this paper
• Production of antibodies against Bacillus thuringiensis delta-endotoxin by injecting its plasmids.

  1994cited by this paper
• Expression of the Neomycin-Resistance (neo) Gene Induces Alterations in Gene Expression and Metabolism

  1994cited by this paper
• An adjustable expression system for controlling growth rate, plasmid maintenance, and culture dynamics

  1992cited by this paper
• Structure and organization of hip, an operon that affects lethality due to inhibition of peptidoglycan or DNA synthesis

  1991cited by this paper
• dif, a recA-independent recombination site in the terminus region of the chromosome of Escherichia coli.

  1991cited by this paper
• Escherichia Coli and Salmonella: Typhimurium Cellular and Molecular Biology

  1987cited by this paper
• Cloning of genes that suppress an Escherichia coli K-12 alanine auxotroph when present in multicopy plasmids

  1987cited by this paper
• [Expression of the chloramphenicol acetyltransferase gene is under control of various promoters of E. coli and phage lambda].

  1986cited by this paper
• Site-directed insertion and deletion mutagenesis with cloned fragments in Escherichia coli

  1985cited by this paper
• Identification and genetic analysis of sbcC mutations in commonly used recBC sbcB strains of Escherichia coli K-12

  1985cited by this paper
• Deletion of an essential gene in Escherichia coli by site-specific recombination with linear DNA fragments

  1984cited by this paper
• Temperature-dependent retention of a tryptophan-operon-bearing plasmid in Escherichia coli.

  1984cited by this paper
• Studies on transformation of Escherichia coli with plasmids.

  1983cited by this paper
• Nucleotide sequence of the kanamycin resistance transposon Tn903.

  1981cited by this paper
• High-efficiency, temperature-sensitive suppression of amber mutations in Escherichia coli

  1981cited by this paper
• A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

  1979cited by this paper
• Cloning of chemically synthesized lactose operators.

  1977cited by this paper
• Genetic analysis of the recF pathway to genetic recombination in Escherichia coli K12: isolation and characterization of mutants.

  1973cited by this paper
• Experiments in molecular genetics

  1972cited by this paper
• NON-INDUCIBLE MUTANTS OF THE REGULATOR GENE IN THE "LACTOSE" SYSTEM OF ESCHERICHIA COLI.

  1964cited by this paper

• Engineered Escherichia coli as a microbial cell factory for intracellular protein delivery: strains, vectors, mechanisms, and therapeutic applications.

  2026cites this paper
• Considerations of Bacterial Robustness and Stability to Improve Bioprocess Design

  2026cites this paper
• The generation game: Toward the generational genetic stability of continuous culture

  2025cites this paper
• De novo 2'-fucosyllactose biosynthesis using glucose as the sole carbon source by multiple engineered Bacillus subtilis.

  2024cites this paper
• Biological Switches: Past and Future Milestones of Transcription Factor-Based Biosensors

  2024cites this paper
• Strengthening microbial cell factories for efficient production of bioactive molecules

  2023cites this paper
• Harnessing xylitol utilization in Escherichia coli for antibiotic-free plasmid and recombinant protein production

  2023cites this paper
• CRISPRi-based programmable logic inverter cascade for antibiotic-free selection and maintenance of multiple plasmids

  2022cites this paper
• Maintenance and gene electrotransfer efficiency of antibiotic resistance gene-free plasmids encoding mouse, canine and human interleukin-12 orthologues

  2022cites this paper
• Recent advances in improving metabolic robustness of microbial cell factories.

  2020cites this paper
• Synthetic symbiosis combining plasmid displacement enables rapid construction of phenotype-stable strains.

  2019cites this paper
• Advances and challenges in recombinant Mycobacterium bovis BCG-based HIV vaccine development: lessons learned

  2018cites this paper
• Gene Electrotransfer of Plasmid-Encoding IL-12 Recruits the M1 Macrophages and Antigen-Presenting Cells Inducing the Eradication of Aggressive B16F10 Murine Melanoma

  2017cites this paper
• Tailor-made transcriptional biosensors for optimizing microbial cell factories

  2017cites this paper
• Advances in Non-Viral DNA Vectors for Gene Therapy

  2017cites this paper
• Tailor-made fibroblast-specific and antibiotic-free interleukin 12 plasmid for gene electrotransfer-mediated cancer immunotherapy.

  2017cites this paper
• Systems Metabolic Engineering of Escherichia coli

  2016cites this paper
• Recombinant protein production in Escherichia coli: optimisation of improved protocols

  2015cites this paper
• Transient Recombinant Protein Expression in Mammalian Cells

  2015cites this paper
• Site Directed Mutagenesis , Expression and Enzymatic Studies of the 60 kDa Human HIV-TAT 1 Interactive Protein , TIP 60

  2015cites this paper
• Design of a Highly Effective Therapeutic HPV16 E6/E7-Specific DNA Vaccine: Optimization by Different Ways of Sequence Rearrangements (Shuffling)

  2014cites this paper
• Plasmid Biopharmaceuticals

  2014cites this paper
• Gene Electrotransfer of Plasmid Antiangiogenic Metargidin Peptide (AMEP) in Disseminated Melanoma: Safety and Efficacy Results of a Phase I First-in-Man Study

  2013cites this paper
• Asd-based balanced-lethal system in attenuated Edwardsiella tarda to express a heterologous antigen for a multivalent bacterial vaccine.

  2013cites this paper
• Safety assessment of biolistic DNA vaccination.

  2013cites this paper
• Overexpression of metK shows different effects on avermectin production in various Streptomyces avermitilis strains

  2013cites this paper
• Building-in biosafety for synthetic biology.

  2013cites this paper
• Operator–Repressor Titration: Stable Plasmid Maintenance without Selectable Marker Genes

  2013cites this paper
• Marker-free plasmids for biotechnological applications - implications and perspectives.

  2013cites this paper
• Overexpression of metK shows different effects on avermectin production in various Streptomyces avermitilis strains

  2013cites this paper
• Successes and failures in modular genetic engineering.

  2012cites this paper
• Pre-Clinical Development of BCG.HIVACAT, an Antibiotic-Free Selection Strain, for HIV-TB Pediatric Vaccine Vectored by Lysine Auxotroph of BCG

  2012cites this paper
• Fast and easy method for construction of plasmid vectors using modified quick-change mutagenesis.

  2012cites this paper
• Plasmid DNA fermentation strategies: influence on plasmid stability and cell physiology

  2012cites this paper
• Design of Biological Systems for Tools and Applications

  2012cites this paper
• Plasmid DNA production for therapeutic applications.

  2012cites this paper
• caling-up recombinant plasmid DNA for clinical trial : Current concern , solution nd status

  2012cites this paper
• Development and Characterization of a novel Immunotherapy against Prostate Cancer

  2012cites this paper
• Strategies for Plasmid DNA Production in Escherichia coli

  2012cites this paper
• Scaling-up recombinant plasmid DNA for clinical trial: current concern, solution and status.

  2012cites this paper
• Building mosaics of therapeutic plasmid gene vectors.

  2011cites this paper
• DNA Cloning in Plasmid Vectors

  2011cites this paper
• New generation of plasmid backbones devoid of antibiotic resistance marker for gene therapy trials.

  2011cites this paper
• Strategies of plasmid DNA production and their influence on therapeutic applications

  2011cites this paper
• Construction of vectors for inducible and constitutive gene expression in Lactobacillus

  2011cites this paper
• The combination of TLR‐9 adjuvantation and electroporation‐mediated delivery enhances in vivo antitumor responses after vaccination with HPV‐16 E7 encoding DNA

  2011cites this paper
• Critical design criteria for minimal antibiotic‐free plasmid vectors necessary to combine robust RNA Pol II and Pol III‐mediated eukaryotic expression with high bacterial production yields

  2010cites this paper
• Novel Antibiotic-Free Plasmid Selection System Based on Complementation of Host Auxotrophy in the NAD De Novo Synthesis Pathway

  2010cites this paper
• Plasmid addiction systems: perspectives and applications in biotechnology

  2010cites this paper
• Development and evaluation of DNA vaccines in chickens against a wild bird H6N2 avian influenza virus from Western Australia

  2010cites this paper
• Biosafety of DNA vaccines: New generation of DNA vectors and current knowledge on the fate of plasmids after injection.

  2010cites this paper
• Evolving Lac repressor for enhanced inducibility.

  2009cites this paper
• An advanced blue-white screening method for construction of shRNA expression vectors.

  2009cites this paper
• Primary capture of high molecular weight nucleic acids using aqueous two-phase systems

  2009cites this paper
• Site Directed Mutagenesis, Expression and Enzymatic Studies of the 60 kDa Human HIV-TAT 1 Interactive Protein, TIP60

  2009cites this paper
• Issues of Large‐Scale Plasmid DNA Manufacturing

  2008cites this paper
• A novel antibiotic free plasmid selection system: Advances in safe and efficient DNA therapy

  2008cites this paper
• Optimizing Expression of Streptococcus pneumoniae Surface Protein a, PspA: Serocross-Reactivity within Families of Antisera Induced Against Clades 1 and 3

  2007cites this paper
• Clinical experience with plasmid DNA- and modified vaccinia virus Ankara-vectored human immunodeficiency virus type 1 clade A vaccine focusing on T-cell induction.

  2007influential citation
• Intragenic vectors for gene transfer without foreign DNA

  2007cites this paper
• Production, characterisation and modification of 1-deoxy-d-xylulose-5-phosphate synthase as a biocatalyst

  2007cites this paper
• An improved rearranged Human Papillomavirus Type 16 E7 DNA vaccine candidate (HPV-16 E7SH) induces an E7 wildtype-specific T cell response.

  2006cites this paper
• Design and preclinical evaluation of a multigene human immunodeficiency virus type 1 subtype C DNA vaccine for clinical trial.

  2006influential citation
• Production of fuculose-1-phosphate aldolase using operator-repressor titration for plasmid maintenance in high cell density Escherichia coli fermentations.

  2005cites this paper
• Human clinical trials of plasmid DNA vaccines.

  2005cites this paper
• Enhanced immunogenicity using an alphavirus replicon DNA vaccine against human immunodeficiency virus type 1.

  2005cites this paper
• Short Communication Enhanced immunogenicity using an alphavirus replicon DNA vaccine against human immunodeficiency virus type 1

  2005cites this paper
• Fermentation Design for the Manufacture of Therapeutic Plasmid DNA

  2005cites this paper
• Separate-component-stabilization system for protein and DNA production without the use of antibiotics.

  2005cites this paper
• BACTERIAL GENE EXPRESSION INHIBITION WITH ANTISENSE PEPTIDE NUCLEIC ACIDS

  2005cites this paper
• Plasmid vaccines and therapeutics: from design to applications.

  2005cites this paper
• From CENTER FOR GENOMICS AND BIOINFORMATICS Karolinska Institutet, Stockholm, Sweden BACTERIAL GENE EXPRESSION INHIBITION WITH ANTISENSE PEPTIDE NUCLEIC ACIDS

  2005cites this paper
• Listeria monocytogenes-Based Antibiotic Resistance Gene-Free Antigen Delivery System Applicable to Other Bacterial Vectors and DNA Vaccines

  2004cites this paper
• Towards the development of a pseudomonas aeruginosa DSM1707 biofilm specific expression system for producing alkaline protease

  2004cites this paper
• Nonviral DNA vectors for immunization and therapy: design and methods for their obtention

  2004cites this paper
• Approaches to Enhance the Efficacy of DNA Vaccines

  2004cites this paper
• A human immunodeficiency virus 1 (HIV-1) clade A vaccine in clinical trials: stimulation of HIV-specific T-cell responses by DNA and recombinant modified vaccinia virus Ankara (MVA) vaccines in humans.

  2004cites this paper
• Engineering RENTA, a DNA prime-MVA boost HIV vaccine tailored for Eastern and Central Africa

  2004cites this paper
• DNA vaccines against human immunodeficiency virus type 1

  2004cites this paper
• Effect of Plasmid Copy Number and lac Operator Sequence on Antibiotic-Free Plasmid Selection by Operator-Repressor Titration in Escherichia coli

  2004cites this paper
• Recombinant Gene Expression

  2004cites this paper
• Plasmid Vectors for Gene Cloning and Expression

  2004cites this paper
• Industrial scale production of plasmid DNA for vaccine and gene therapy: plasmid design, production, and purification

  2003cites this paper
• Antibiotic-free bacterial strain selection using antisense peptide nucleic acid.

  2003cites this paper
• MINIREVIEW: Perspectives on plasmid‐based gene therapy: challenges for the product and the process

  2003cites this paper
• A modified Escherichia coli protein production strain expressing staphylococcal nuclease, capable of auto-hydrolysing host nucleic acid.

  2003cites this paper
• Technical issues in construction of nucleic acid vaccines.

  2003cites this paper
• A LysR-family transcriptional regulator is involved in the selenium-dependent transcriptional regpression of selenium-free hydrogenase gene groups in Methanococcus voltae

  2003cites this paper
• Ligation of exogenous linear DNA after gene transfer in vitro and in vivo

  2003cites this paper
• Genetic Transformation and Hybridization

  2002cites this paper
• Lack of toxicity and persistence in the mouse associated with administration of candidate DNA- and modified vaccinia virus Ankara (MVA)-based HIV vaccines for Kenya.

  2002cites this paper
• Purification of plasmids for gene therapy and DNA vaccination.

  2001cites this paper
• Purification of essentially RNA free plasmid DNA using a modified Escherichia coli host strain expressing ribonuclease A.

  2001cites this paper
• A non-antibiotic marker for amplification of plant transformation vectors in E. coli

  2001cites this paper
• Gene therapy: progress and challenges.

  2001cites this paper
• Advances in Escherichia coli production of therapeutic proteins.

  2001cites this paper
• Escherichia coli strains that allow antibiotic-free plasmid selection and maintenance by repressor titration.

  2001cites this paper
• Antibiotic-Free Plasmid Selection and Maintenance in Bacteria

  2001cites this paper
• Chapter 20. Issues of Large-Scale Plasmid DNA Manufacturing

  2001cites this paper
• Expression of Escherichia coli B nitroreductase in established human tumor xenografts in mice results in potent antitumoral and bystander effects upon systemic administration of the prodrug CB1954

  2000cites this paper