Functions of PIWI proteins in spermatogenesis

Recently, a significant understanding of the molecular mechanisms regulating spermatogenesis has been achieved utilizing small RNA molecules (small RNAs), including small interfering RNAs (siRNAs), microRNAs (miRNAs), and Piwi-interacting RNAs (piRNAs) which emerged as important regulators of gene expression at the post-transcriptional or translation level. piRNAs are only present in pachytene spermatocytes and round spermatids, whereas miRNAs are expressed abundantly in male germ cells throughout spermatogenesis. This review is aimed at providing a glimpse of piRNAs and their interacting family proteins such as PIWIL1, PIWIL2, and PIWIL4 in spermatogenesis.

• Publication year

  2011

• Venue

  Clinical and Experimental Reproductive Medicine

• Publication date

  2011-06-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.5653/cerm.2011.38.2.61PMID 22384420PMCID 3283061
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Argonautes ALG-3 and ALG-4 are required for spermatogenesis-specific 26G-RNAs and thermotolerant sperm in Caenorhabditis elegans

  2010cited by this paper
• Mouse MOV10L1 associates with Piwi proteins and is an essential component of the Piwi-interacting RNA (piRNA) pathway

  2010influential reference
• Proteomic analysis of murine Piwi proteins reveals a role for arginine methylation in specifying interaction with Tudor family members.

  2009cited by this paper
• The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline.

  2009influential reference
• Loss of the Mili-interacting Tudor domain–containing protein-1 activates transposons and alters the Mili-associated small RNA profile

  2009cited by this paper
• SnapShot: mouse piRNAs, PIWI proteins, and the ping-pong cycle.

  2009cited by this paper
• The biogenesis and function of PIWI proteins and piRNAs: progress and prospect.

  2009influential reference
• SnapShot: Fly piRNAs, PIWI proteins, and the ping-pong cycle.

  2009cited by this paper
• Mice Deficient for a Small Cluster of Piwi-Interacting RNAs Implicate Piwi-Interacting RNAs in Transposon Control1

  2008cited by this paper
• A C. elegans Piwi, PRG-1, regulates 21U-RNAs during spermatogenesis.

  2008cited by this paper
• A piRNA pathway primed by individual transposons is linked to de novo DNA methylation in mice.

  2008influential reference
• MicroRNA Biogenesis Is Required for Mouse Primordial Germ Cell Development and Spermatogenesis

  2008cited by this paper
• PRG-1 and 21U-RNAs interact to form the piRNA complex required for fertility in C. elegans.

  2008cited by this paper
• DNA methylation of retrotransposon genes is regulated by Piwi family members MILI and MIWI2 in murine fetal testes.

  2008influential reference
• A Slicer-Mediated Mechanism for Repeat-Associated siRNA 5' End Formation in Drosophila

  2007influential reference
• MIWI2 is essential for spermatogenesis and repression of transposons in the mouse male germline.

  2007influential reference
• piRNAs in the Germ Line

  2007influential reference
• Biogenesis and germline functions of piRNAs

  2007cited by this paper
• Slicer and the argonautes.

  2007cited by this paper
• Computational Analysis of Mouse piRNA Sequence and Biogenesis

  2007cited by this paper
• Transposable elements and the epigenetic regulation of the genome

  2007cited by this paper
• Noncoding RNAs of the Mammalian Testis

  2007cited by this paper
• Developmentally Regulated piRNA Clusters Implicate MILI in Transposon Control

  2007cited by this paper
• Discrete small RNA-generating loci as master regulators of transposon activity in Drosophila.

  2007influential reference
• Identification and characterization of two novel classes of small RNAs in the mouse germline: retrotransposon-derived siRNAs in oocytes and germline small RNAs in testes.

  2006cited by this paper
• A germline-specific class of small RNAs binds mammalian Piwi proteins

  2006cited by this paper
• A novel class of small RNAs bind to MILI protein in mouse testes

  2006cited by this paper
• Micro RNAs in animal development.

  2006cited by this paper
• A novel class of small RNAs in mouse spermatogenic cells.

  2006cited by this paper
• MIWI associates with translational machinery and PIWI-interacting RNAs (piRNAs) in regulating spermatogenesis

  2006cited by this paper
• Characterization of the piRNA Complex from Rat Testes

  2006cited by this paper
• A Distinct Small RNA Pathway Silences Selfish Genetic Elements in the Germline

  2006influential reference
• SMEDWI-2 Is a PIWI-Like Protein That Regulates Planarian Stem Cells

  2005cited by this paper
• Meiotic catastrophe and retrotransposon reactivation in male germ cells lacking Dnmt3L

  2004cited by this paper
• Mobile Elements: Drivers of Genome Evolution

  2004cited by this paper
• RISC assembly defects in the Drosophila RNAi mutant armitage.

  2004cited by this paper
• Mili, a mammalian member of piwi family gene, is essential for spermatogenesis

  2004cited by this paper
• Identification of eight members of the Argonaute family in the human genome.

  2003cited by this paper
• Mouse Germ Cell-Less as an Essential Component for Nuclear Integrity

  2003cited by this paper
• Initial sequencing and comparative analysis of the mouse genome.

  2002cited by this paper
• Male germ cell gene expression.

  2002cited by this paper
• miwi, a murine homolog of piwi, encodes a cytoplasmic protein essential for spermatogenesis.

  2002influential reference
• Initial sequencing and comparative analysis of the mouse genome

  2002cited by this paper
• Yb modulates the divisions of both germline and somatic stem cells through piwi- and hh-mediated mechanisms in the Drosophila ovary.

  2001cited by this paper
• Two mouse piwi-related genes: miwi and mili.

  2001influential reference
• Initial sequencing and analysis of the human genome

  2001cited by this paper
• DExD/H box RNA helicases: from generic motors to specific dissociation functions.

  2001cited by this paper
• The mouse homolog of Drosophila Vasa is required for the development of male germ cells.

  2000cited by this paper
• Transcription of IAP endogenous retroviruses is constrained by cytosine methylation

  1998cited by this paper
• A novel class of evolutionarily conserved genes defined by piwi are essential for stem cell self-renewal.

  1998influential reference
• A novel group of pumilio mutations affects the asymmetric division of germline stem cells in the Drosophila ovary.

  1997cited by this paper
• Regulated synthesis, transport and assembly of the Drosophila germ plasm.

  1996cited by this paper
• Targeted mutation of the DNA methyltransferase gene results in embryonic lethality.

  1992cited by this paper
• Histological and Histopathological Evaluation of the Testis

  1990cited by this paper
• The biology of the sperm cell.

  1975cited by this paper

• Spatiotemporal single-cell architecture of gene expression in the Caenorhabditis elegans germ cells

  2025cites this paper
• Chronic unpredictable stress exposure disrupts testicular function by modulating germ cell-junctional dynamics and Nrf2/HO-1/IKKβ/NF-κB pathway.

  2025cites this paper
• Sex determination factor, a novel male-linked gene in the sea cucumber Apostichopus japonicus: Molecular characterization, expression patterns and effects of gene knockdown.

  2025cites this paper
• Epigenetics in reproductive infertility: A review

  2024cites this paper
• Molecular mechanisms of cellular dysfunction in testes from men with non-obstructive azoospermia

  2023cites this paper
• Functions and mechanism of noncoding RNA in regulation and differentiation of male mammalian reproduction

  2023cites this paper
• Paternal source of germ plasm determinants in the viviparous teleost, Gambusia holbrooki; dads do matter.

  2023cites this paper
• Quercetin inhibits testicular toxicity induced by the mixture of three commonly used phthalates in rats.

  2022cites this paper
• Immunolocalization of Vasa, PIWI, and TDRKH proteins in male germ cells during spermatogenesis of the teleost fish Poecilia reticulata.

  2022cites this paper
• Structural basis for RNA 3'-end recognition by the PIWIL2 PAZ domain.

  2021cites this paper
• Polyubiquitin gene Ubb is required for upregulation of Piwi protein level during mouse testis development

  2021cites this paper
• PAZ domain is critical for spermatogenesis in chicken

  2021cites this paper
• Changes in Expressions of Spermatogenic Marker Genes and Spermatogenic Cell Population Caused by Stress

  2021cites this paper
• Lower Spermatozoal PIWI-LIKE 1 and 2 Transcript Levels Are Significantly Associated with Higher Fertilization Rates in IVF

  2021cites this paper
• Associations between infant sex and DNA methylation across umbilical cord blood, artery, and placenta samples

  2021cites this paper
• Polyubiquitin gene Ubb is required for upregulation of Piwi protein level during mouse testis development

  2021cites this paper
• Maternal Immune Activation and Retrotransposition in Neurodevelopmental Disorders.

  2021cites this paper
• An old weapon with a new function: PIWI-interacting RNAs in neurodegenerative diseases

  2021influential citation
• TACG_A_207491 1..11

  2020cites this paper
• Identification and characterization of two piwi genes and their expression in response to E2 (17β-estradiol) in Dabry’s sturgeon Acipenser dabryanus

  2020cites this paper
• PAZ Domain is Critical for PIWIL1 Function During Spermatogenesis in Chicken

  2020cites this paper
• Pde8b haploinsufficiency in mice is associated with modest adrenal defects, impaired steroidogenesis, and male infertility, unaltered by concurrent PKA or Wnt activation.

  2020cites this paper
• Identification and functional characterization of piwi1 gene in sea cucumber, Apostichopus japonicas.

  2020cites this paper
• Estrogen-ERα signaling and DNA hypomethylation co-regulate expression of stem cell protein PIWIL1 in ERα-positive endometrial cancer cells

  2020cites this paper
• The Importance of Small Non-Coding RNAs in Human Reproduction: A Review Article

  2020cites this paper
• Zinc supplement ameliorates phthalates-induced reproductive toxicity in male rats.

  2020cites this paper
• The dynamics of gene expression during and post meiosis sets the sperm agenda

  2019cites this paper
• Proteomic analysis of Tianzhu White Yak (Bos grunniens) testis at different sexual developmental stages.

  2019cites this paper
• Somatic expression of piRNA and associated machinery in the mouse identifies short, tissue-specific piRNA

  2019cites this paper
• Management, Treatment, and Molecular Background of the Growing Teratoma Syndrome.

  2019cites this paper
• Single nucleotide polymorphisms in piRNA-pathway genes: an insight into genetic determinants of human diseases

  2019cites this paper
• Transcriptome Sequencing Reveals the Traits of Spermatogenesis and Testicular Development in Large Yellow Croaker (Larimichthys crocea)

  2019cites this paper
• Promoter DNA methylation and expression analysis of PIWIL1 gene in purebred and crossbred cattle bulls

  2019cites this paper
• Regulatory Functions of MicroRNAs in Male Reproductive Health: A New Approach to Understanding Male Infertility.

  2018cites this paper
• Differential expression of two Piwil orthologs during embryonic and gonadal development in pufferfish, Takifugu fasciatus.

  2018cites this paper
• GLIS1–3 transcription factors: critical roles in the regulation of multiple physiological processes and diseases

  2018cites this paper
• PIWI-Etkileşimli RNA’ların Biyogenezi ve İşlevleri

  2018cites this paper
• Transit-Amplifying Cells in the Fast Lane from Stem Cells towards Differentiation

  2017cites this paper
• Male Infertility: An Epigenetic Perspective

  2017cites this paper
• Unraveling transcriptome dynamics in human spermatogenesis

  2017influential citation
• Altered PIWI-LIKE 1 and PIWI-LIKE 2 mRNA expression in ejaculated spermatozoa of men with impaired sperm characteristics

  2017cites this paper
• Transcriptome analysis of the gonads of olive flounder (Paralichthys olivaceus)

  2016cites this paper
• Epigenetics: A key paradigm in reproductive health

  2016cites this paper
• Molecular cloning and expression analysis of piwil1 gene in Acipenser sinensis

  2016cites this paper
• Co-expression of Piwil2/Piwil4 in nucleus indicates poor prognosis of hepatocellular carcinoma

  2016cites this paper
• The role of epigenetics in idiopathic male infertility

  2016cites this paper
• Veränderungen der DNA-Methylierung bei männlicher Infertilität

  2015cites this paper
• Tudor Domain Containing Protein TDRD12 Expresses at the Acrosome of Spermatids in Mouse Testis

  2015cites this paper
• Cloning, expression and methylation analysis of piwil2 in half-smooth tongue sole (Cynoglossus semilaevis).

  2014cites this paper
• Expression Profiles of PIWIL2 Short Isoforms Differ in Testicular Germ Cell Tumors of Various Differentiation Subtypes

  2014cites this paper
• Array-based DNA methylation profiling in male infertility reveals allele-specific DNA methylation in PIWIL1 and PIWIL2.

  2014cites this paper
• Transcriptional specificity in various p53-mutant cells.

  2013cites this paper
• Towards the identification of sex-determining gene(s) in channel catfish

  2013cites this paper
• The molecular evolution of reproduction in animals: insights from sexual and asexual rotifers

  2013cites this paper
• The Interplay of Coding and Non-Coding Regulations in Mouse Spermatogenesis

  2013cites this paper
• Suppression effect of LHRH-A and hCG on Piwi expression in testis of Nile tilapia Oreochromis niloticus.

  2013cites this paper
• Broad DNA methylation changes of spermatogenesis, inflammation and immune response-related genes in a subgroup of sperm samples for assisted reproduction

  2013influential citation
• Male-Biased Genes in Catfish as Revealed by RNA-Seq Analysis of the Testis Transcriptome

  2013cites this paper
• Inventory and phylogenetic analysis of meiotic genes in monogonont rotifers.

  2013cites this paper
• Molecular control of rodent spermatogenesis.

  2012cites this paper
• Inventory and Phylogenetic Analysis of Meiotic Genes in Monogonont Rotifers Materials and Methods Monogonont Genome Sequence Generation and Annotation Pcr-stitching of Monogonont Genes Results and Discussion Hanson Et Al. @bullet Phylogenetic Analysis of Meiotic Genes in Monogonont Rotifers Conclusi

  year unknowncites this paper