A Novel Tetrodotoxin-sensitive, Voltage-gated Sodium Channel Expressed in Rat and Human Dorsal Root Ganglia*

Dorsal root ganglion neurons express a wide repertoire of sodium channels with different properties. Here, we report the cloning from rat, dorsal root ganglia (DRG), cellular expression, and functional analysis of a novel tetrodotoxin-sensitive peripheral sodium channel (PN), PN1. PN1 mRNA is expressed in many different tissues. Within the rat DRG, both the mRNA and PN1-like immunoreactivity are present in small and large neurons. The abundance of sodium channel mRNAs in rat DRG is rBI > PN1 ≥ PN3 >>> rBIII by quantitative reverse transcription-polymerase chain reaction analysis. Data from reverse transcription-polymerase chain reaction and sequence analyses of human DRG and other human tissues suggest that rat PN1 is an ortholog of the human neuroendocrine channel. In Xenopus oocytes, PN1 exhibits kinetics that are similar to rBIIa sodium currents and is inhibited by tetrodotoxin with an IC50 of 4.3 ± 0.92 nm. Unlike rBIIa, the inactivation kinetics of PN1 are not accelerated by the coexpression of the β-subunits.

• Publication year

  1997

• Venue

  Journal of Biological Chemistry

• Publication date

  1997-06-06

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1074/JBC.272.23.14805PMID 9169448
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• RT-PCR and sequence analyses of human dorsal root ganglia and other human tissues suggest that rat PN1 is an ortholog of the human neuroendocrine channel.

  Confidence 0.84

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) reviewimjlk (vdp8mqzes2) review
• Unlike rBIIa, PN1 inactivation is not accelerated by coexpression of β-subunits.

  Confidence 0.90

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) reviewimjlk (vdp8mqzes2) review
• In Xenopus oocytes, PN1 shows gating kinetics similar to rBIIa sodium currents and is blocked by tetrodotoxin with an IC50 of 4.3 ± 0.92 nM.

  Confidence 0.93

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) reviewimjlk (vdp8mqzes2) review
• PN1 mRNA and PN1-like immunoreactivity are present in both small and large neurons within rat dorsal root ganglia.

  Confidence 0.95

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) reviewimjlk (vdp8mqzes2) review
• PN1 was cloned from rat dorsal root ganglia as a novel tetrodotoxin-sensitive peripheral sodium channel.

  Confidence 0.98

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) reviewimjlk (vdp8mqzes2) review

• human neuroendocrine channel

  ion channel

  A human sodium-channel sequence or channel type inferred from PCR and sequence comparisons with rat PN1.

  Aliases: human NE channel

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) reviewimjlk (vdp8mqzes2) review
• pn1

  ion channel

  A rat peripheral voltage-gated sodium channel isoform cloned and characterized in dorsal root ganglia and Xenopus oocytes.

  Aliases: peripheral sodium channel PN1

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) reviewimjlk (vdp8mqzes2) review
• rat dorsal root ganglia

  tissue

  The rat sensory ganglion tissue used as the source for cloning and expression analysis in this work.

  Aliases: rat DRG, DRG

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) reviewimjlk (vdp8mqzes2) review
• rbiia sodium currents

  electrophysiological phenotype

  A rat brain sodium-current phenotype used as a functional comparison for PN1 in oocyte recordings.

  Aliases: rBIIa

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) reviewimjlk (vdp8mqzes2) review
• tetrodotoxin

  chemical

  A potent sodium-channel blocker used here to assess channel sensitivity by inhibition assays.

  Aliases: TTX

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) reviewimjlk (vdp8mqzes2) review
• β-subunits

  protein subunits

  Auxiliary sodium-channel subunits coexpressed with the channel in Xenopus oocytes to test effects on inactivation.

  Aliases: beta-subunits

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) reviewimjlk (vdp8mqzes2) review

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