Immunological dissection of human Ia molecules

The immunochemical analysis of Daudi Ia molecules by a variety of alloantisera led to the recognition of at least three molecular species carrying different antigenic determinants: DRw6, DC-1, and DC-2. Genetic as well as structural evidence indicates that DRw6 and DC-1 molecules are controlled by separate, HLA-linked loci, rather than by alleles at the same locus. The alloantigenic determinants appear to be expressed on the small Ia subunit. DC-1 and DC-2 determinants discussed had not been defined by serological analysis at the population level, but were demonstrated to be present by immunochemical analysis at the molecular level.

• Publication year

  1978

• Venue

  Journal of Experimental Medicine

• Publication date

  1978-12-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1084/JEM.148.6.1592PMID 82605PMCID 2185093
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  1961influential reference

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  1992cites this paper
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