Holliday junctions accumulate in replication mutants via a RecA homolog-independent mechanism.

The Holliday junction recombination intermediate, an X-shaped DNA molecule (xDNA), was analyzed at rDNA in mitotically growing yeast. In wild-type cells, xDNA is only detected at S phase, suggesting that recombination is stimulated to repair replication-related lesions. A search for mutations that increase the level of xDNA uncovered a gene encoding a subunit of DNA polymerase alpha. Systematic examination of replication mutants revealed that defects in polymerase alpha and delta but not the epsilon complex stimulate the level of xDNA. These xDNAs are Holliday junctions and not replication intermediates. The level of Holliday junctions is greatly reduced in rad52 mutants, but surprisingly, not in mutants defective in the three known mitotically expressed yeast RecA homologs.

• Publication year

  1997

• Venue

  Cell

• Publication date

  1997-07-11

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/S0092-8674(00)80316-5PMID 9230305
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Replicon size of yeast ribosomal DNA

  2004cited by this paper
• Yeast Rad55 and Rad57 proteins form a heterodimer that functions with replication protein A to promote DNA strand exchange by Rad51 recombinase.

  1997cited by this paper
• Is RecF a DNA replication protein?

  1997cited by this paper
• A Rad52 homolog is required for RAD51-independent mitotic recombination in Saccharomyces cerevisiae.

  1996cited by this paper
• DNA strand annealing is promoted by the yeast Rad52 protein.

  1996cited by this paper
• Gene conversion plays the major role in controlling the stability of large tandem repeats in yeast.

  1996cited by this paper
• Single-stranded DNA arising at telomeres in cdc13 mutants may constitute a specific signal for the RAD9 checkpoint

  1995cited by this paper
• Hyper-recombination and Bloom's syndrome: microbes again provide clues about cancer.

  1995cited by this paper
• DNA structure-dependent requirements for yeast RAD genes in gene conversion

  1995cited by this paper
• Characterization of a mutant strain of Saccharomyces cerevisiae with a deletion of the RAD27 gene, a structural homolog of the RAD2 nucleotide excision repair gene

  1995influential reference
• DNA polymerase epsilon links the DNA replication machinery to the S phase checkpoint.

  1995influential reference
• DNA strand exchange mediated by a RAD51-ssDNA nucleoprotein filament with polarity opposite to that of RecA.

  1995cited by this paper
• Identification of double Holliday junctions as intermediates in meiotic recombination.

  1995cited by this paper
• Yeast DNA polymerases and their role at the replication fork.

  1995cited by this paper
• The mechanism of recA polA lethality: suppression by RecA-independent recombination repair activated by the lexA(Def) mutation in Escherichia coli.

  1995cited by this paper
• Genetic control of intrachromosomal recombination

  1995cited by this paper
• Multiple pathways for homologous recombination in Saccharomyces cerevisiae.

  1995influential reference
• Collapse and repair of replication forks in Escherichia coli

  1995cited by this paper
• Destabilization of tracts of simple repetitive DNA in yeast by mutations affecting DNA mismatch repair

  1994cited by this paper
• DNA replication triggered by double-stranded breaks in E. coli: dependence on homologous recombination functions.

  1994cited by this paper
• Homologous pairing and strand exchange promoted by the Escherichia coli RecT protein.

  1994cited by this paper
• The kinetics of spontaneous DNA branch migration.

  1994cited by this paper
• Homologous recombination of monkey alpha-satellite repeats in an in vitro simian virus 40 replication system: possible association of recombination with DNA replication

  1994cited by this paper
• Analysis of an origin of DNA amplification in Sciara coprophila by a novel three-dimensional gel method

  1994cited by this paper
• Sequence of the RAD55 gene of Saccharomyces cerevisiae: similarity of RAD55 to prokaryotic RecA and other RecA-like proteins.

  1994influential reference
• Cloning, overexpression, purification, and characterization of the Escherichia coli RuvC Holliday junction resolvase.

  1994cited by this paper
• Unrepaired heteroduplex DNA in Saccharomyces cerevisiae is decreased in RAD1 RAD52-independent recombination.

  1994influential reference
• Biochemistry of homologous recombination in Escherichia coli.

  1994cited by this paper
• The B subunit of the DNA polymerase alpha-primase complex in Saccharomyces cerevisiae executes an essential function at the initial stage of DNA replication

  1994cited by this paper
• A mammalian protein complex that repairs double-strand breaks and deletions by recombination.

  1993cited by this paper
• DNA polymerases delta and epsilon are required for chromosomal replication in Saccharomyces cerevisiae

  1993cited by this paper
• Yeast DNA replication.

  1993cited by this paper
• Destabilization of tracts of simple repetitive DNA in yeast by mutations affecting DNA mismatch repair

  1993cited by this paper
• DNA Polymerases 8andEAreRequired forChromosomal Replication inSaccharomyces cerevisiae

  1993cited by this paper
• ATP-dependent recognition of eukaryotic origins of DNA replication by a multiprotein complex

  1992cited by this paper
• Transposon Tn5 excision in yeast: influence of DNA polymerases alpha, delta, and epsilon and repair genes.

  1992cited by this paper
• Rad51 protein involved in repair and recombination in S. cerevisiae is a RecA-like protein.

  1992influential reference
• DNA polymerase II, the probable homolog of mammalian DNA polymerase epsilon, replicates chromosomal DNA in the yeast Saccharomyces cerevisiae.

  1992cited by this paper
• Sister chromatids are preferred over homologs as substrates for recombinational repair in Saccharomyces cerevisiae.

  1992cited by this paper
• DMC1: a meiosis-specific yeast homolog of E. coli recA required for recombination, synaptonemal complex formation, and cell cycle progression.

  1992cited by this paper
• Mismatch-stimulated plasmid integration in yeast

  1991cited by this paper
• Nucleotide sequence of the RAD57 gene of Saccharomyces cerevisiae.

  1991influential reference
• 8 Recombination in Yeast

  1991cited by this paper
• Positional mapping of genes by chromosome blotting and chromosome fragmentation.

  1991influential reference
• The CDC20 gene product of Saccharomyces cerevisiae, a beta-transducin homolog, is required for a subset of microtubule-dependent cellular processes

  1991cited by this paper
• DPB2, the gene encoding DNA polymerase II subunit B, is required for chromosome replication in Saccharomyces cerevisiae.

  1991cited by this paper
• Intermediates of recombination during mating type switching in Saccharomyces cerevisiae.

  1990cited by this paper
• Mismatch repair-induced meiotic recombination requires the pms1 gene product.

  1990cited by this paper
• A third essential DNA polymerase in S. cerevisiae.

  1990cited by this paper
• Elevated recombination rates in transcriptionally active DNA.

  1989cited by this paper
• Disruption of the RAD52 gene alters the spectrum of spontaneous SUP4-o mutations in Saccharomyces cerevisiae.

  1989cited by this paper
• A replication fork barrier at the 3' end of yeast ribosomal RNA genes.

  1988cited by this paper
• The localization of replication origins on ARS plasmids in S. cerevisiae.

  1987cited by this paper
• Methods in yeast genetics (laboratory course manual): By F. Sherman, G. R. Fink, and J. B. Hicks, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 1987. 186 pp. Paper bound, $25.00

  1987influential reference
• Recombinational substrates designed to study recombination between unique and repetitive sequences in vivo.

  1987cited by this paper
• Electron microscopic study of Saccharomyces cerevisiae rDNA chromatin replication

  1986cited by this paper
• DNA polymerase I and DNA primase complex in yeast.

  1984cited by this paper
• Replication and recombination in adenovirus-infected cells are temporally and functionally related

  1984cited by this paper
• The double-strand-break repair model for recombination.

  1983cited by this paper
• Mitotic chromosome loss in a radiation-sensitive strain of the yeast Saccharomyces cerevisiae.

  1981cited by this paper
• Spontaneous mitotic recombination in mms8-1, an allele of the CDC9 gene of Saccharomyces cerevisiae

  1981influential reference
• Unequal crossing over in the ribosomal DNA of Saccharomyces cerevisiae

  1980cited by this paper
• Methods in yeast genetics

  1979cited by this paper
• A model for replication repair in mammalian cells.

  1976cited by this paper
• A Replicator’s View of Recombination (and Repair)

  1974cited by this paper
• Recombination in yeast.

  1971cited by this paper
• Exchanges between DNA strands in ultraviolet-irradiated Escherichia coli.

  1971cited by this paper
• Macromolecule Synthesis in Temperature-sensitive Mutants of Yeast

  1967cited by this paper

• Helicase activities of Rad5 and Rrm3 genetically interact in the prevention of recombinogenic DNA lesions in Saccharomyces cerevisiae.

  2023cites this paper
• Synthetic cell-cycle regulation identifies Mif2CENP-C as a CDK phospho-target at the kinetochore

  2023cites this paper
• Interrogating the substrate specificity landscape of UvrC reveals novel insights into its noncanonical function.

  2022cites this paper
• ScreenGarden: a shinyR application for fast and easy analysis of plate-based high-throughput screens

  2021cites this paper
• Forced association of SARS-CoV-2 proteins with the yeast proteome perturb vesicle trafficking

  2021cites this paper
• Analysis of the Replication Mechanisms of the Human Papillomavirus Genomes

  2021cites this paper
• The Analysis of Recombination-Dependent Processing of Blocked Replication Forks by Bidimensional Gel Electrophoresis.

  2021cites this paper
• Non-Recombinogenic Functions of Rad51, BRCA2, and Rad52 in DNA Damage Tolerance

  2021cites this paper
• Characterisation of homologous recombination induced by replication inhibition in mammalian cells

  2020cites this paper
• 2D Gel Electrophoresis to Detect DNA Replication and Recombination Intermediates in Budding Yeast.

  2020cites this paper
• CDK and Mec1/Tel1-catalyzed phosphorylation of Sae2 regulate different responses to DNA damage

  2019cites this paper
• Studying the effects of DNA replication stress on genome integrity and cell viability

  2018cites this paper
• Topoisomerase 3α Is Required for Decatenation and Segregation of Human mtDNA.

  2018cites this paper
• Sae2 antagonizes Rad9 accumulation at DNA double-strand breaks to attenuate checkpoint signaling and facilitate end resection

  2018influential citation
• RPA and RAD51: fork reversal, fork protection, and genome stability

  2018cites this paper
• Localization of GFP-Rad 52 in stable human cell lines

  2017cites this paper
• Double-strand breaks are not the main cause of spontaneous sister chromatid exchange in wild-type yeast cells

  2017influential citation
• Pathways and Mechanisms that Prevent Genome Instability in Saccharomyces cerevisiae

  2017cites this paper
• Genome-wide mapping of sister chromatid exchange events in single yeast cells using Strand-seq

  2017influential citation
• S-phase checkpoint regulations that preserve replication and chromosome integrity upon dNTP depletion

  2017cites this paper
• Explorer Cells expressing murine RAD 52 splice variants favor sister chromatid repair

  2017cites this paper
• Absence of Non-histone Protein Complexes at Natural Chromosomal Pause Sites Results in Reduced Replication Pausing in Aging Yeast Cells.

  2016cites this paper
• Synthetic protein interactions reveal a functional map of the cell

  2016cites this paper
• DNA damage tolerance by recombination: Molecular pathways and DNA structures

  2016cites this paper
• Fluorescent foci quantitation for high-throughput analysis.

  2015cites this paper
• Top2 and Sgs1-Top3 Act Redundantly to Ensure rDNA Replication Termination

  2015cites this paper
• Fluorescent foci quantitation for high-throughput

  2015cites this paper
• Synthetic physical interactions map kinetochore regulators and regions sensitive to constitutive Cdc14 localization

  2015cites this paper
• Mus81-Mms4 and Yen1 resolve a novel anaphase bridge formed by noncanonical Holliday junctions

  2014cites this paper
• The Rad5 helicase activity is dispensable for error-free DNA post-replication repair.

  2014cites this paper
• Rad52/Rad59-dependent Recombination as a Means to Rectify Faulty Okazaki Fragment Processing*

  2014cites this paper
• Mechanisms and Regulation of Mitotic Recombination in Saccharomyces cerevisiae

  2014cites this paper
• Homologous recombination as a mechanism to recognize repetitive DNA sequences in an RNAi pathway.

  2013cites this paper
• Friedreich's ataxia–associated GAA repeats induce replication-fork reversal and unusual molecular junctions

  2013cites this paper
• MRX protects fork integrity at protein–DNA barriers, and its absence causes checkpoint activation dependent on chromatin context

  2013cites this paper
• Alleles of the homologous recombination gene, RAD59, identify multiple responses to disrupted DNA replication in Saccharomyces cerevisiae

  2013cites this paper
• Nucleosome assembly and genome integrity

  2012cites this paper
• © 2012 Landes Bioscience. Do not distribute. © 2012 Landes Bioscience. Do not distribute.

  2012cites this paper
• Complex Chromosomal Rearrangements Mediated by Break-Induced Replication Involve Structure-Selective Endonucleases

  2012cites this paper
• Cohesin Proteins Promote Ribosomal RNA Production and Protein Translation in Yeast and Human Cells

  2012influential citation
• Genome instability induced by structured DNA and replication fork restart

  2012cites this paper
• DNA Polymerase α (swi7) and the Flap Endonuclease Fen1 (rad2) Act Together in the S-Phase Alkylation Damage Response in S. pombe

  2012cites this paper
• Homologous Recombination as a Replication Fork Escort: Fork-Protection and Recovery

  2012cites this paper
• RECQ1 is required for cellular resistance to replication stress and catalyzes strand exchange on stalled replication fork structures

  2012cites this paper
• Increased chromosome mobility facilitates homology search during recombination

  2012cites this paper
• The intra-S phase checkpoint targets Dna2 to prevent stalled replication forks from reversing.

  2012cites this paper
• Intermediates of DNA double strand break repair in escherichia coli

  2012cites this paper
• Replicative Age Induces Mitotic Recombination in the Ribosomal RNA Gene Cluster of Saccharomyces cerevisiae

  2011cites this paper
• RAD51-independent inverted-repeat recombination by a strand-annealing mechanism.

  2011cites this paper
• Meiotic Recombination Intermediates Are Resolved with Minimal Crossover Formation during Return-to-Growth, an Analogue of the Mitotic Cell Cycle

  2011cites this paper
• Replication fork stability in mammalian cells

  2011cites this paper
• Born to be Alive: A Role for the BCL-2 Family in Melanoma Tumor Cell Survival, Apoptosis, and Treatment

  2011cites this paper
• Visualization of eukaryotic DNA mismatch repair reveals distinct recognition and repair intermediates.

  2011cites this paper
• The SUMO Isopeptidase Ulp2p Is Required to Prevent Recombination-Induced Chromosome Segregation Lethality following DNA Replication Stress

  2011cites this paper
• A large scale genomic screen reveals mechanisms of yeast postreplication repair in Saccharomyces cerevisiae

  2011cites this paper
• Monitoring the rate and dynamics of concerted evolution in the ribosomal DNA repeats of Saccharomyces cerevisiae using experimental evolution.

  2011cites this paper
• Homologous recombination restarts blocked replication forks at the expense of genome rearrangements by template exchange.

  2010cites this paper
• Extensive DNA End Processing by Exo1 and Sgs1 Inhibits Break-Induced Replication

  2010cites this paper
• RNase-dependent discontinuities associated with the crossovers of spontaneously formed joint DNA molecules in Physarum polycephalum

  2010cites this paper
• Function of TopBP1 in genome stability.

  2010cites this paper
• Multiple Rad5 activities mediate sister chromatid recombination to bypass DNA damage at stalled replication forks.

  2010influential citation
• Abundance of Ribosomal RNA Gene Copies Maintains Genome Integrity

  2010cites this paper
• The double life of Holliday junctions

  2010cites this paper
• The replication fork's five degrees of freedom, their failure and genome rearrangements.

  2009cites this paper
• The Carboxyl Terminus of Brca2 Links the Disassembly of Rad51 Complexes to Mitotic Entry

  2009cites this paper
• Contrasting Roles of Checkpoint Proteins as Recombination Modulators at Fob1-Ter Complexes with or without Fork Arrest

  2009cites this paper
• Replication fork reversal and the maintenance of genome stability

  2009cites this paper
• Regulation of rDNA stability by sumoylation.

  2009cites this paper
• Chromatin assembly controls replication fork stability

  2009cites this paper
• Specific pathways prevent duplication-mediated genome rearrangements

  2009cites this paper
• Telomeres do the (un)twist: helicase actions at chromosome termini.

  2009cites this paper
• Suppression of the Double-Strand-Break-Repair Defect of the Saccharomyces cerevisiae rad57 Mutant

  2009cites this paper
• Contrasting Roles of Checkpoint Proteins as Recombination Modulators at Fob1-<italic toggle='yes'>Ter</italic> Complexes with or without Fork Arrest

  2009cites this paper
• Comparative Genomics and Molecular Dynamics of DNA Repeats in Eukaryotes

  2008cites this paper
• Low Levels of DNA Polymerase Alpha Induce Mitotic and Meiotic Instability in the Ribosomal DNA Gene Cluster of Saccharomyces cerevisiae

  2008cites this paper
• Rad52 multimerization is important for its nuclear localization in Saccharomyces cerevisiae.

  2008cites this paper
• Rad52 multimerization is important for its nuclear localization in S. cerevisiae

  2008cites this paper
• Mutagenic and Recombinagenic Responses to Defective DNA Polymerase δ Are Facilitated by the Rev1 Protein in pol3-t Mutants of Saccharomyces cerevisiae

  2008cites this paper
• Sae2, Exo1 and Sgs1 collaborate in DNA double-strand break processing

  2008cites this paper
• Replication fork arrest, recombination and the maintenance of ribosomal DNA stability.

  2008cites this paper
• Modeling stem cell asymmetry in yeast.

  2008cites this paper
• Involvement of homologous recombination repair after proton-induced DNA damage.

  2008cites this paper
• Nucleolar Localization and Dynamic Roles of Flap Endonuclease 1 in Ribosomal DNA Replication and Damage Repair

  2008cites this paper
• How the Genome Is Organized in the Glomeromycota

  2007cites this paper
• Schizosaccharomyces pombe

  2007cites this paper
• Top1- and Top2-mediated topological transitions at replication forks ensure fork progression and stability and prevent DNA damage checkpoint activation.

  2007cites this paper
• Evidence That a RecQ Helicase Slows Senescence by Resolving Recombining Telomeres

  2007cites this paper
• Rad51-Independent Interchromosomal Double-Strand Break Repair by Gene Conversion Requires Rad52 but Not Rad55, Rad57, or Dmc1

  2007cites this paper
• THE FHCRC/NCI YEAST ANTICANCER DRUG SCREEN

  2007cites this paper
• Mms22 Preserves Genomic Integrity During DNA Replication in Schizosaccharomyces pombe

  2007cites this paper
• More is not always better: the genetic constraints of polyploidy.

  2007cites this paper
• Yeast mother cell-specific ageing, genetic (in)stability, and the somatic mutation theory of ageing

  2007cites this paper
• Mutations affecting a putative MutLalpha endonuclease motif impact multiple mismatch repair functions.

  2007cites this paper
• Arrested replication fork processing: interplay between checkpoints and recombination.

  2007cites this paper
• Sister chromatid recombination

  2007cites this paper
• Chromosome segregation and double-strand break repair - a complex connection.

  2007cites this paper
• Inverted DNA Repeats Channel Repair of Distant Double-Strand Breaks into Chromatid Fusions and Chromosomal Rearrangements

  2007cites this paper
• Evidence That a RecQ Helicase Slows

  2007cites this paper
• Suppression of spontaneous genome rearrangements in yeast DNA helicase mutants

  2006cites this paper
• The Bloom's Syndrome Helicase Can Promote the Regression of a Model Replication Fork*

  2006cites this paper