Macrophage receptors of polysaccharide isolated from a marine filamentous fungus Phoma herbarum YS4108

AbstractAim:YCP, a novel (1,4)-α-D-glucan, was isolated from the mycelium of the marine filamentous fungus Phoma herbarum YS4108. In this work, we investigated a YCP-binding cellular receptor expressed by macrophages and the intracellular signal transduction pathways involved in YCP-induced macrophage activation.Methods:Fluorescence-labeled YCP (fl-YCP) was prepared using the CDAP-activation method. Fluorescence confocal laser microscopy and fluorescence-activated cell sorting (FACS) were used to analyze the effect of fl-YCP on macrophages. To characterize the properties of the YCP receptor, carbohydrates and antibodies were used to inhibit the binding of fl-YCP to macrophages. Moreover, we investigated the role of membrane receptors Toll-like receptor 2 (TLR2), Toll-like receptor 4 (TLR4), Toll-like receptor 6 (TLR6) and complement receptor 3 (CR3). We also examined the role of the p38 kinase pathway in mediating nitric oxide (NO) production.Results:YCP had an in vitro stimulatory effect on the release of NO in macrophage, and fl-YCP can bind directly to receptors on the surface of macrophages in a time- and dose-dependent manner. Competition studies show that LPS, laminarin, anti-TLR4 antibody and anti-CD11b (CR3) antibody could inhibit fl-YCP binding to macrophages. Conversely, mannose, anti-TLR2 and anti-TLR6 antibody could not. Treatment of RAW264.7 cells with YCP resulted in significant activation of p38 in a time-dependent manner. The specific p38 inhibitor SB203580 abrogated YCP-induced NO generation. Treatment of RAW264.7 cells with anti-TLR4 antibody and anti-CR3 antibody significantly reduced YCP-induced NO production and p38 activation.Conclusion:We have demonstrated that YCP-induced NO production occurs through the TLR4 and CR3 membrane receptors in a p38 kinase-dependent manner in macrophages.

• Publication year

  2009

• Venue

  Acta Pharmacologica Sinica

• Publication date

  2009-07-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1038/aps.2009.93PMID 19575004PMCID 4006659
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• C-type lectins and phagocytosis

  2009cited by this paper
• C-reactive protein induces high-mobility group box-1 protein release through activation of p38MAPK in macrophage RAW264.7 cells.

  2008cited by this paper
• Inhibition of LPS-induced NO and PGE2 production by asiatic acid via NF-kappa B inactivation in RAW 264.7 macrophages: possible involvement of the IKK and MAPK pathways.

  2008cited by this paper
• Screening of the types of immunocytes bound to YCP obtained from marine filamentous Phoma herbarum YS4108 in vitro

  2008cited by this paper
• Upregulation of vascular endothelial growth factor by heat-killed Listeria monocytogenes in macrophages.

  2007cited by this paper
• Concanavalin A induced expression of Toll-like receptors in murine peritoneal macrophages in vitro.

  2007cited by this paper
• The anti-inflammatory effects of Pyrolae herba extract through the inhibition of the expression of inducible nitric oxide synthase (iNOS) and NO production.

  2007cited by this paper
• TLR2 and TLR4 agonists synergistically up-regulate SR-A in RAW264.7 through p38.

  2007cited by this paper
• Lipopolysaccharide primes macrophages to increase nitric oxide production in response to Staphylococcus aureus.

  2007cited by this paper
• Angelan isolated from Angelica gigas Nakai induces dendritic cell maturation through toll-like receptor 4.

  2007cited by this paper
• Mechanism of macrophage activation by (1,4)-α-d-glucan isolated from Tinospora cordifolia

  2006cited by this paper
• Complement and Toll-like receptors: key regulators of adaptive immune responses.

  2006cited by this paper
• Mechanism of macrophage activation by (1,4)-alpha-D-glucan isolated from Tinospora cordifolia.

  2006cited by this paper
• Sulfation of a polysaccharide produced by a marine filamentous fungus Phoma herbarum YS4108 alters its antioxidant properties in vitro.

  2005cited by this paper
• Purification, characterization and enzymatic degradation of YCP, a polysaccharide from marine filamentous fungus Phoma herbarum YS4108.

  2005cited by this paper
• Receptor-mediated stimulatory effect of oligochitosan in macrophages.

  2004cited by this paper
• Bacterial endotoxin induces IL-20 expression in the glial cells.

  2004cited by this paper
• Polysaccharide isolated from Poria cocos sclerotium induces NF-kappaB/Rel activation and iNOS expression through the activation of p38 kinase in murine macrophages.

  2004cited by this paper
• Immuno-enhancing Effect of YCP Obtained from A Marine Filamentous Fungus Keissleriella sp. YS 4108 on Mice Peritoneal Macrophage

  2004cited by this paper
• Activation of mitogen-activated protein kinases and AP-1 by polysaccharide isolated from the radix of Platycodon grandiflorum in RAW 264.7 cells.

  2004cited by this paper
• Toll-like receptor 4-dependent activation of macrophages by polysaccharide isolated from the radix of Platycodon grandiflorum.

  2003cited by this paper
• Toll-like receptor-mediated activation of B cells and macrophages by polysaccharide isolated from cell culture of Acanthopanax senticosus.

  2003cited by this paper
• Candida albicans mannan-protein conjugate as vaccine candidate.

  2003cited by this paper
• Toll receptors, CD14, and macrophage activation and deactivation by LPS.

  2002cited by this paper
• Dectin-1 Is A Major β-Glucan Receptor On Macrophages

  2002cited by this paper
• Activation of mitogen-activated protein kinase pathways by angelan in murine macrophages.

  2001cited by this paper
• Activation of soluble polysaccharides with 1-cyano-4-dimethylaminopyridinium tetrafluoroborate (CDAP) for use in protein-polysaccharide conjugate vaccines and immunological reagents. II. Selective crosslinking of proteins to CDAP-activated polysaccharides.

  2000cited by this paper
• Staphylococcus aureus

  1948cited by this paper

• The biomedical frontier of fucoidan and laminarin: emerging insights

  2025cites this paper
• Glucans and applications in drug delivery.

  2024cites this paper
• Chemical profile of craft brewer’s spent yeast and its antioxidant and antiproliferative activities

  2023cites this paper
• Structural characterization and anti-inflammatory activity of polysaccharides from Astragalus membranaceus.

  2023cites this paper
• Bioinspired yeast-based β-glucan system for oral drug delivery.

  2023cites this paper
• Polysaccharides from sporoderm-removed spores of Ganoderma lucidum induce apoptosis in human gastric cancer cells via disruption of autophagic flux

  2021cites this paper
• The Genus Phoma: A Review of Its Potential Bioactivities, Implications, and Prospects

  2021cites this paper
• AN INVESTIGATION OF THE CELLULAR UPTAKE AND MECHANISMS OF ACTION OF MGN-3 ON INFLAMMATORY PROCESSES

  2019influential citation
• Ingestion of Non-digestible Carbohydrates From Plant-Source Foods and Decreased Risk of Colorectal Cancer: A Review on the Biological Effects and the Mechanisms of Action

  2019cites this paper
• Marine-derived Phoma—the gold mine of bioactive compounds

  2018cites this paper
• Bronchodilator effects of Lignosus rhinocerotis extract on rat isolated airways is linked to the blockage of calcium entry.

  2018cites this paper
• Rhizopus nigricans polysaccharide activated macrophages and suppressed tumor growth in CT26 tumor-bearing mice.

  2018cites this paper
• Polysaccharide rich extract (PRE) from Tinospora cordifolia inhibits the intracellular survival of drug resistant strains of Mycobacterium tuberculosis in macrophages by nitric oxide induction.

  2018cites this paper
• Non-starch polysaccharide from Chinese yam activated RAW 264.7 macrophages through the Toll-like receptor 4 (TLR4)-NF-κB signaling pathway

  2017cites this paper
• Marine Microbial Polysaccharides Environmental role and Applications: An Overview

  2017cites this paper
• The novel α-glucan YCP improves the survival rates and symptoms in septic mice by regulating myeloid-derived suppressor cells

  2017cites this paper
• Detection of Nitric Oxide from Living Cells Using Polymeric Zinc Organic Framework-Derived Zinc Oxide Composite with Conducting Polymer.

  2017cites this paper
• Activation of murine macrophages by G1‐4A, a polysaccharide from Tinospora cordifolia, in TLR4/MyD88 dependent manner

  2017cites this paper
• Polysaccharides from Ganoderma lucidum Promote Cognitive Function and Neural Progenitor Proliferation in Mouse Model of Alzheimer's Disease

  2017cites this paper
• Alhagi-honey and its compound abnormal savda munziq ( ASMq ) inhibit proliferation and enhance apoptosis of human hepatoma BEL-7402 cells

  2016cites this paper
• Lipopolysaccharide quantification and alkali-based inactivation in polysaccharide preparations to enable in vitro immune modulatory studies

  2016cites this paper
• G1-4A, a Polysaccharide from Tinospora cordifolia Inhibits the Survival of Mycobacterium tuberculosis by Modulating Host Immune Responses in TLR4 Dependent Manner

  2016cites this paper
• Structural characterization and immunomodulatory activity of a new heteropolysaccharide from Prunella vulgaris.

  2015cites this paper
• Activation of RAW 264.7 cells by a polysaccharide isolated from Antarctic bacterium Pseudoaltermonas sp. S-5.

  2015cites this paper
• Characterization, antioxidant and immunomodulatory activities of polysaccharides from Prunella vulgaris Linn.

  2015cites this paper
• Inflammatory Modulation Effect of Glycopeptide from Ganoderma capense (Lloyd) Teng

  2014cites this paper
• Immunomodulatory Effects of Polysaccharide from Marine Fungus Phoma herbarum YS4108 on T Cells and Dendritic Cells

  2014cites this paper
• Preparation, optimization and application of affinity absorbent with a polysaccharide YCP as the ligand.

  2014cites this paper
• Preparation and immunomodulating activities of a library of low-molecular-weight α-glucans.

  2014cites this paper
• Toll-Like Receptor 2 and Toll-Like Receptor 4-Dependent Activation of B Cells by a Polysaccharide from Marine Fungus Phoma herbarum YS4108

  2013cites this paper
• Developing Phytocompounds from Medicinal Plants as Immunomodulators

  2012cites this paper
• A polysaccharide from Strongylocentrotus nudus eggs protects against myelosuppression and immunosuppression in cyclophosphamide-treated mice.

  2011cites this paper
• Enzymatic degradation products from a marine polysaccharide YCP with different immunological activity and binding affinity to macrophages, hydrolyzed by alpha-amylases from different origins.

  2010cites this paper