Split-inteins and their bioapplications

No abstract is available for this paper.

• Publication year

  2015

• Venue

  Biotechnology Letters

• Publication date

  2015-07-08

• Fields of study

  Biology, Medicine, Materials Science

• Identifiers
  DOI 10.1007/s10529-015-1905-2PMID 26153348
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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• Manipulating L-type calcium channels in cardiomyocytes using split-intein protein transsplicing

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• Two- and three-input TALE-based AND logic computation in embryonic stem cells

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  2012cited by this paper
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• Protein Trans-Splicing of an Atypical Split Intein Showing Structural Flexibility and Cross-Reactivity

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• Unprecedented Rates and Efficiencies Revealed for New Natural Split Inteins from Metagenomic Sources*

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• Circular Permutation Prediction Reveals a Viable Backbone Disconnection for Split Proteins: An Approach in Identifying a New Functional Split Intein

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• Solution structure of DnaE intein from Nostoc punctiforme: Structural basis for the design of a new split intein suitable for site‐specific chemical modification

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• The naturally split Npu DnaE intein exhibits an extraordinarily high rate in the protein trans‐splicing reaction

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• Segmental isotopic labeling of a central domain in a multidomain protein by protein trans-splicing using only one robust DnaE intein.

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• In Vivo and In Vitro Protein Ligation by Naturally Occurring and Engineered Split DnaE Inteins

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• Protein C-Terminal Labeling and Biotinylation Using Synthetic Peptide and Split-Intein

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• Novel Split Intein for trans-Splicing Synthetic Peptide onto C Terminus of Protein*

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• Site‐Specific Chemical Modification of Proteins with a Prelabelled Cysteine Tag Using the Artificially Split Mxe GyrA Intein

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• Segmental Isotopic Labelling of a Multidomain Protein by Protein Ligation by Protein Trans‐Splicing

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• Activation of protein splicing with light in yeast

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• Activation of protein splicing by protease- or light-triggered O to N acyl migration.

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• Semisynthetic murine prion protein equipped with a GPI anchor mimic incorporates into cellular membranes.

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• A genetic method to identify mitochondrial proteins in living mammalian cells.

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• Expression of split dnaE genes and trans-splicing of DnaE intein in the developmental cyanobacterium Anabaena sp. PCC 7120.

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• Highly efficient protein trans‐splicing by a naturally split DnaE intein from Nostoc punctiforme

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