Quantitative real-time RT-PCR--a perspective.

No abstract is available for this paper.

• Publication year

  2005

• Venue

  Journal of Molecular Endocrinology

• Publication date

  2005-06-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1677/JME.1.01755PMID 15956331
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  2002influential reference
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