Fluorescently labeled ribosomes as a tool for analyzing antibiotic binding.

No abstract is available for this paper.

• Publication year

  2009

• Venue

  RNA: A publication of the RNA Society

• Publication date

  2009-08-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1261/rna.1681609PMID 19553343PMCID PMC2714759
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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• Potential New Antibiotic Sites in the Ribosome Revealed by Deleterious Mutations in RNA of the Large Ribosomal Subunit*

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  2005influential reference
• A protein component at the heart of an RNA machine: the importance of protein l27 for the function of the bacterial ribosome.

  2005cited by this paper
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  2002cited by this paper
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  2000cited by this paper
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  2000cited by this paper
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  2000cited by this paper
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  1997cited by this paper
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  1996cited by this paper
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  1996cited by this paper
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  1992cited by this paper
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  1991cited by this paper
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  1987cited by this paper
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  1987cited by this paper
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  1986cited by this paper
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  1985cited by this paper
• Photoaffinity labeling of the pactamycin binding site on eubacterial ribosomes.

  1985cited by this paper
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  1979cited by this paper
• Mechanism of action of aminoglycoside antibiotics. Binding studies of tobramycin and its 6'-N-acetyl derivative to the bacterial ribosome and its subunits.

  1979influential reference
• Interaction of kanamycin and related antibiotics with the large subunit of ribosomes and the inhibition of translocation.

  1978cited by this paper
• Fluorescent Assay for Estimating the Binding of Erythromycin Derivatives to Ribosomes

  1978cited by this paper
• Interaction of a fluorescent streptomycin derivative with Escherichia coli ribosomes.

  1977cited by this paper
• Paromomycin and dihydrostreptomycin binding to Escherichia coli ribosomes.

  1976cited by this paper
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  1973cited by this paper
• Binding of Dihydrostreptomycin to Escherichia coli Ribosomes: Characteristics and Equilibrium of the Reaction

  1972cited by this paper

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  2017cites this paper
• Fluorescence-switching RNA for detection of bacterial ribosomes.

  2017cites this paper
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  2017cites this paper
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  2016cites this paper
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