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The Arabidopsis alkaline ceramidase TOD1 is a key turgor pressure regulator in plant cells

Li-yu Chen,Dongqiao Shi,Wen-Juan Zhang,Zuoshun Tang,Jie Liu,Wei-cai Yang

Published 2015 in Nature Communications

ABSTRACT

Turgor pressure plays pivotal roles in the growth and movement of walled cells that make up plants and fungi. However, the molecular mechanisms regulating turgor pressure and the coordination between turgor pressure and cell wall remodelling for cell growth remain poorly understood. Here, we report the characterization of Arabidopsis TurgOr regulation Defect 1 (TOD1), which is preferentially expressed in pollen tubes and silique guard cells. We demonstrate that TOD1 is a Golgi-localized alkaline ceramidase. tod1 mutant pollen tubes have higher turgor than wild type and show growth retardation both in pistils and in agarose medium. In addition, tod1 guard cells are insensitive to abscisic acid (ABA)-induced stomatal closure, whereas sphingosine-1-phosphate, a putative downstream component of ABA signalling and product of alkaline ceramidases, promotes closure in both wild type and tod1. Our data suggest that TOD1 acts in turgor pressure regulation in both guard cells and pollen tubes. Turgor pressure is critical for the growth of plant cells but the mechanisms regulating turgor are poorly understood. Here, Chen et al. identify TOD1, an alkaline ceramidase, involved in sphingosine metabolism that regulates turgor during pollen tube growth and stomatal closure.

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