Functional Characterization of a Higher Plant Sphingolipid Δ4-Desaturase: Defining the Role of Sphingosine and Sphingosine-1-Phosphate in Arabidopsis1[W][OA]

The role of Δ4-unsaturated sphingolipid long-chain bases such as sphingosine was investigated in Arabidopsis (Arabidopsis thaliana). Identification and functional characterization of the sole Arabidopsis ortholog of the sphingolipid Δ4-desaturase was achieved by heterologous expression in Pichia pastoris. A P. pastoris mutant disrupted in the endogenous sphingolipid Δ4-desaturase gene was unable to synthesize glucosylceramides. Synthesis of glucosylceramides was restored by the expression of Arabidopsis gene At4g04930, and these sphingolipids were shown to contain Δ4-unsaturated long-chain bases, confirming that this open reading frame encodes the sphingolipid Δ4-desaturase. At4g04930 has a very restricted expression pattern, transcripts only being detected in pollen and floral tissues. Arabidopsis insertion mutants disrupted in the sphingolipid Δ4-desaturase At4g04930 were isolated and found to be phenotypically normal. Sphingolipidomic profiling of a T-DNA insertion mutant indicated the absence of Δ4-unsaturated sphingolipids in floral tissue, also resulting in the reduced accumulation of glucosylceramides. No difference in the response to drought or water loss was observed between wild-type plants and insertion mutants disrupted in the sphingolipid Δ4-desaturase At4g04930, nor was any difference observed in stomatal closure after treatment with abscisic acid. No differences in pollen viability between wild-type plants and insertion mutants were detected. Based on these observations, it seems unlikely that Δ4-unsaturated sphingolipids and their metabolites such as sphingosine-1-phosphate play a significant role in Arabidopsis growth and development. However, Δ4-unsaturated ceramides may play a previously unrecognized role in the channeling of substrates for the synthesis of glucosylceramides.

• Publication year

  2008

• Venue

  Plant Physiology

• Publication date

  2008-10-31

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1104/pp.108.129411PMID 18978071PMCID PMC2613699
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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• Sphingolipid Long-Chain Base Hydroxylation Is Important for Growth and Regulation of Sphingolipid Content and Composition in Arabidopsis[W]

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• The very-long-chain hydroxy fatty acyl-CoA dehydratase PASTICCINO2 is essential and limiting for plant development

  2008influential reference
• Involvement of sphingosine kinase in plant cell signalling.

  2008cited by this paper
• Serine Palmitoyltransferase, a Key Enzyme for de Novo Synthesis of Sphingolipids, Is Essential for Male Gametophyte Development in Arabidopsis1[W][OA]

  2008cited by this paper
• A Higher Plant Δ8 Sphingolipid Desaturase with a Preference for (Z)-Isomer Formation Confers Aluminum Tolerance to Yeast and Plants[C][OA]

  2007cited by this paper
• An “Electronic Fluorescent Pictograph” Browser for Exploring and Analyzing Large-Scale Biological Data Sets

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• Involvement of sphingoid bases in mediating reactive oxygen intermediate production and programmed cell death in Arabidopsis

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• Rapid measurement of sphingolipids from Arabidopsis thaliana by reversed-phase high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry.

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• The COMATOSE ATP-Binding Cassette Transporter Is Required for Full Fertility in Arabidopsis1[W][OA]

  2007influential reference
• Faculty Opinions recommendation of Genome-wide identification and testing of superior reference genes for transcript normalization in Arabidopsis.

  2006cited by this paper
• A trial of phenome analysis using 4000 Ds-insertional mutants in gene-coding regions of Arabidopsis.

  2006influential reference
• Separation and Identification of Major Plant Sphingolipid Classes from Leaves*

  2006influential reference
• The Essential Nature of Sphingolipids in Plants as Revealed by the Functional Identification and Characterization of the Arabidopsis LCB1 Subunit of Serine Palmitoyltransferase[W]

  2006cited by this paper
• Identification of Fungal Sphingolipid C9-methyltransferases by Phylogenetic Profiling*

  2006cited by this paper
• Arabidopsis Sphingosine Kinase and the Effects of Phytosphingosine-1-Phosphate on Stomatal Aperture1[w]

  2005cited by this paper
• Genome-Wide Identification and Testing of Superior Reference Genes for Transcript Normalization in Arabidopsis1[w]

  2005cited by this paper
• Analysis of Detergent-Resistant Membranes in Arabidopsis. Evidence for Plasma Membrane Lipid Rafts1

  2005cited by this paper
• Characterization of two GL8 paralogs reveals that the 3-ketoacyl reductase component of fatty acid elongase is essential for maize (Zea mays L.) development.

  2005cited by this paper
• Disruptions of the Arabidopsis Enoyl-CoA Reductase Gene Reveal an Essential Role for Very-Long-Chain Fatty Acid Synthesis in Cell Expansion during Plant Morphogenesis

  2005cited by this paper
• Fatty acid desaturases from the microalga Thalassiosira pseudonana

  2005cited by this paper
• Drought tolerance established by enhanced expression of the CC-NBS-LRR gene, ADR1, requires salicylic acid, EDS1 and ABI1.

  2004cited by this paper
• An introduction to plant sphingolipids and a review of recent advances in understanding their metabolism and function.

  2004cited by this paper
• Point-Counterpoint of Sphingosine 1-Phosphate Metabolism

  2004cited by this paper
• Death and taxis: what non-mammalian models tell us about sphingosine-1-phosphate.

  2004cited by this paper
• A post-genomic approach to understanding sphingolipid metabolism in Arabidopsis thaliana.

  2004cited by this paper
• Lipid Metabolism and Membrane Biogenesis

  2004cited by this paper
• Sphingolipids, new players in plant signaling.

  2003influential reference
• Sphingosine-1-phosphate: an enigmatic signalling lipid

  2003cited by this paper
• Genome-Wide Insertional Mutagenesis of Arabidopsis thaliana

  2003influential reference
• Ceramides modulate programmed cell death in plants.

  2003cited by this paper
• The dihydroceramide desaturase is not essential for cell viability in Schizosaccharomyces pombe

  2003cited by this paper
• Sphingolipid signalling in Arabidopsis guard cells involves heterotrimeric G proteins

  2003influential reference
• Plant sphingolipids: structural diversity, biosynthesis, first genes and functions.

  2003cited by this paper
• A new class of lipid desaturase central to sphingolipid biosynthesis and signalling.

  2002cited by this paper
• Identification and Characterization of a Sphingolipid Δ4-Desaturase Family*

  2002influential reference
• Identification and characterization of a sphingolipid delta 4-desaturase family.

  2002cited by this paper
• Functional characterization of sphingolipid C4‐hydroxylase genes from Arabidopsis thaliana

  2001cited by this paper
• Modulation of abscisic acid signal transduction and biosynthesis by an Sm-like protein in Arabidopsis.

  2001cited by this paper
• Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.

  2001cited by this paper
• Intracellular signalling: sphingosine-1-phosphate branches out.

  2001influential reference
• Drought-induced guard cell signal transduction involves sphingosine-1-phosphate

  2001influential reference
• Structure determination of soybean and wheat glucosylceramides by tandem mass spectrometry.

  2000cited by this paper
• Sphingosine 1-phosphate signalling in mammalian cells.

  2000cited by this paper
• Sphingoid Base Composition of Cerebrosides from Plant Leaves

  1997cited by this paper
• Characterization of Ceramide Synthesis

  1997cited by this paper
• The Critical Requirement for Linolenic Acid Is Pollen Development, Not Photosynthesis, in an Arabidopsis Mutant.

  1996cited by this paper
• Isolation and characterization of abscisic acid-deficient Arabidopsis mutants at two new loci.

  1996cited by this paper
• degenerative spermatocyte, a novel gene encoding a transmembrane protein required for the initiation of meiosis in Drosophila spermatogenesis

  1996cited by this paper
• Literature cited

  1924cited by this paper
• Sphingolipid Mediators in Cardiovascular Cell Biology and Pathology

  year unknowncited by this paper

• Membranes, Metabolism, and Messages: How Plant Lipids Drive Growth and Climate Resilience

  2026cites this paper
• Genome-wide characterization of sphingolipid metabolism pathway under abiotic stresses reveals BnaCERK playing a positive role in drought resistance in Brassica napus.

  2025cites this paper
• Functional characterization of a plastidial cytochrome b5-fused Δ4-desaturase from Ostreococcus tauri in higher plants.

  2025cites this paper
• Multi-omics insights into the molecular basis of powdery mildew resistance and root metabolic variation in Astragalus membranaceus var. mongholicus

  2025cites this paper
• Sphingolipid metabolism and signaling in plant stress responses and crop improvement.

  2025cites this paper
• Characterisation of Unique Eukaryotic Sphingolipids with Temperature-Dependent Δ8-Unsaturation from the Picoalga Ostreococcus Tauri.

  2024cites this paper
• Engineering of Saccharomyces cerevisiae as a platform strain for microbial production of sphingosine-1-phosphate

  2024cites this paper
• Sphingolipid homeostasis--how do cells know when enough is enough? Implications for plant pathogen responses.

  2024cites this paper
• Microbiology Combined with the Root Metabolome Reveals the Responses of Root Microorganisms to Maize Cultivars under Different Forms of Nitrogen Supply

  2024cites this paper
• Genetic and molecular basis of carotenoid metabolism in cereals

  2023cites this paper
• Complex sphingolipid profiling and identification of an inositol-phosphorylceramide synthase in Dictyostelium discoideum

  2023cites this paper
• Alternative PCR-Based Approaches for Generation of Komagataella phaffii Strains

  2023cites this paper
• Diversity in Sphingolipid Metabolism across Land Plants.

  2022influential citation
• Metabolism of crown tissue is crucial for drought tolerance and recovery after stress cessation in Lolium/Festuca forage grasses.

  2022cites this paper
• Sphingolipids in plant immunity

  2022cites this paper
• Diversity in sphingolipid metabolism across land plants

  2022influential citation
• Characterization of Ceramides and Glucosylceramides of the Satsuma Mandarin(Citrus unshiu) Fruit.

  2022cites this paper
• Genome-wide identification of FAD gene family and their contributions to the temperature stresses and mutualistic and parasitic fungi colonization responses in banana.

  2022cites this paper
• Plant monounsaturated fatty acids: Diversity, biosynthesis, functions and uses.

  2021cites this paper
• Sphingolipid metabolism, transport, and functions in plants: Recent progress and future perspectives

  2021cites this paper
• Disruption of long‐chain base hydroxylation alters growth and impacts sphingolipid synthesis in Physcomitrella patens

  2021cites this paper
• Sphingolipid Δ4-desaturation is an important metabolic step for glycosylceramide formation in Physcomitrium patens

  2021influential citation
• Link between Lipid Second Messengers and Osmotic Stress in Plants

  2021cites this paper
• Komagataella phaffii as Emerging Model Organism in Fundamental Research

  2021cites this paper
• Arabidopsis MHP1, a homologue of yeast Mpo1, is involved in ABA signaling.

  2020cites this paper
• LARP6C regulates selective mRNA translation to promote pollen tube guidance in Arabidopsis thaliana

  2020cites this paper
• Sphingolipids in plants: a guidebook on their function in membrane architecture, cellular processes, and environmental or developmental responses

  2020cites this paper
• Integral Membrane Fatty Acid Desaturases: A Review of Biochemical, Structural, and Biotechnological Advances

  2020cites this paper
• Dissecting the regulatory roles of ORM proteins in the sphingolipid pathway of plants

  2020cites this paper
• Phytosphingosine induces systemic acquired resistance through activation of sphingosine kinase

  2020cites this paper
• Genome-wide identification and comparative analysis of alternative splicing across four legume species

  2019cites this paper
• Whole-genome mining and in silico analysis of FAD gene family in Brassica juncea

  2019cites this paper
• Biosynthesis of long chain base in sphingolipids in animals, plants and fungi

  2019cites this paper
• Prediction of regulatory long intergenic non-coding RNAs acting in trans through base-pairing interactions

  2019cites this paper
• Sphingolipids: towards an integrated view of metabolism during the plant stress response

  2019cites this paper
• Genome-wide identification, characterization and in-silico profiling of genes encoding FAD (fatty acid desaturase) proteins in chickpea (Cicer arietinum L.)

  2019cites this paper
• Identification of Fatty Acid Desaturases in Maize and Their Differential Responses to Low and High Temperature

  2019cites this paper
• Plant-Unique cis/trans Isomerism of Long-Chain Base Unsaturation is Selectively Required for Aluminum Tolerance Resulting from Glucosylceramide-Dependent Plasma Membrane Fluidity

  2019cites this paper
• Emerging Roles of Sphingolipid Signaling in Plant Response to Biotic and Abiotic Stresses.

  2018cites this paper
• Modulation of sphingolipid long-chain base composition and gene expression during early olive-fruit development, and putative role of brassinosteroid.

  2018cites this paper
• Genome-Wide Survey and Characterization of Fatty Acid Desaturase Gene Family in Brassica napus and Its Parental Species

  2018cites this paper
• The role of phytosphingosine-1-phosphate (Phyto-S1P) and its relationships with cytosolic pH and hydrogen peroxide (H2O2) during stomatal closure by darkness in broad bean

  2017cites this paper
• Synthesis and degradation of long-chain base phosphates affect fumonisin B1-induced cell death in Arabidopsis thaliana

  2017cites this paper
• Isolation and functional analysis of fatty acid desaturase genes from peanut (Arachis hypogaea L.)

  2017cites this paper
• Classification and substrate head-group specificity of membrane fatty acid desaturases

  2016cites this paper
• Genes encoding Δ8-sphingolipid desaturase from various plants: identification, biochemical functions, and evolution

  2016cites this paper
• Substrate specificity, kinetic properties and inhibition by fumonisin B1 of ceramide synthase isoforms from Arabidopsis.

  2016cites this paper
• Sphingolipids as targets for treatment of fungal infections.

  2016cites this paper
• Plant sphingolipids: Their importance in cellular organization and adaption

  2016cites this paper
• Stomatal closure induced by phytosphingosine-1-phosphate and sphingosine-1-phosphate depends on nitric oxide and pH of guard cells in Pisum sativum

  2016cites this paper
• The sphingolipid biosynthetic enzyme Sphingolipid delta8 desaturase is important for chilling resistance of tomato

  2016cites this paper
• Lipids in pollen - They are different.

  2016cites this paper
• Lipids in Plant and Algae Development

  2016cites this paper
• Plant Sphingolipid Metabolism and Function.

  2016cites this paper
• Molecular characterization and targeted quantitative profiling of the sphingolipidome in rice.

  2016cites this paper
• Studies on the structure and function of fatty acid desaturase

  2016cites this paper
• Bioinformatics-based approaches to engineer the transmembrane Δ6 desaturase from Micromonas pusilla

  2016cites this paper
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  2015cites this paper
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  2015cites this paper
• Ethylene Modulates Sphingolipid Synthesis in Arabidopsis

  2015cites this paper
• Sphingolipid metabolism is strikingly different between pollen and leaf in Arabidopsis as revealed by compositional and gene expression profiling.

  2015cites this paper
• The Arabidopsis alkaline ceramidase TOD1 is a key turgor pressure regulator in plant cells

  2015cites this paper
• Long-chain bases, phosphatidic acid, MAPKs, and reactive oxygen species as nodal signal transducers in stress responses in Arabidopsis

  2015cites this paper
• Investigation of Pathways for Complex Sphingolipid Biosynthesis in Arabidopsis thaliana (L.) Heynh

  2015influential citation
• Physiological significance of glycolipid catabolism in cryptococcus neoformans

  2015cites this paper
• Development of an LC–MS/MS Method for the Analysis of Free Sphingoid Bases Using 4-Fluoro-7-nitrobenzofurazan (NBD-F)

  2014cites this paper
• Molecular characterization of rice sphingosine-1-phosphate lyase gene OsSPL1 and functional analysis of its role in disease resistance response

  2014cites this paper
• SPHINGOLIPID VARIETY, BIOSYNTHESIS AND REGULATION

  2014cites this paper
• Sterylglucoside Catabolism in Cryptococcus neoformans with Endoglycoceramidase-related Protein 2 (EGCrP2), the First Steryl-β-glucosidase Identified in Fungi*

  2014cites this paper
• Long-chain base phosphates modulate pollen tube growth via channel-mediated influx of calcium.

  2014cites this paper
• Advanced LC-MS/MS Techniques Dissecting Diverse Isomers of Plant Sphingolipid Species

  2014cites this paper
• Association Mapping of Seed Oil and Protein Content in Sesamum indicum L. Using SSR Markers

  2014cites this paper
• Arabidopsis mutants in sphingolipid synthesis as tools to understand the structure and function of membrane microdomains in plasmodesmata

  2013cites this paper
• Acyl-Lipid Metabolism

  2013cites this paper
• Cytochrome b5 Reductase Encoded by CBR1 Is Essential for a Functional Male Gametophyte in Arabidopsis[C][W]

  2013cites this paper
• Abnormal Glycosphingolipid Mannosylation Triggers Salicylic Acid–Mediated Responses in Arabidopsis[W][OA]

  2013cites this paper
• Regulatory role of membrane fluidity in gene expression and physiological functions

  2013cites this paper
• Overexpression of a rice long-chain base kinase gene OsLCBK1 in tobacco improves oxidative stress tolerance

  2013cites this paper
• Plant sphingolipids: function follows form.

  2013cites this paper
• Overexpression of rice sphingosine-1-phoshpate lyase gene OsSPL1 in transgenic tobacco reduces salt and oxidative stress tolerance.

  2012cites this paper
• Characterization of bifunctional sphingolipid Δ4-desaturases/C4-hydroxylases of trypanosomatids by liquid chromatography-electrospray tandem mass spectrometry.

  2012cites this paper
• Evidence that sphingolipid signaling is involved in responding to low temperature.

  2012cites this paper
• Dihydroceramide desaturase and dihydrosphingolipids: debutant players in the sphingolipid arena.

  2012cites this paper
• Sphingosine-1-phosphate (S1P) mediates darkness-induced stomatal closure through raising cytosol pH and hydrogen peroxide (H2O2) levels in guard cells in Vicia faba

  2012cites this paper
• Lipids of plant membrane rafts.

  2012cites this paper
• Isolation and functional characterisation of the genes encoding Δ(8)-sphingolipid desaturase from Brassica rapa.

  2012cites this paper
• Connections between Sphingosine Kinase and Phospholipase D in the Abscisic Acid Signaling Pathway in Arabidopsis*

  2012cites this paper
• Arabidopsis mutants of sphingolipid fatty acid α-hydroxylases accumulate ceramides and salicylates.

  2012cites this paper
• Sphingosine in plants--more riddles from the Sphinx?

  2012cites this paper
• Phytosphingosine-phosphate is a signal for AtMPK6 activation and Arabidopsis response to chilling.

  2012cites this paper
• Lipid profiling of the model temperate grass, Brachypodium distachyon

  2012influential citation
• Sphingolipids and Plant Defense/Disease: The “Death” Connection and Beyond

  2012cites this paper
• Crosstalk between Phospholipase D and Sphingosine Kinase in Plant Stress Signaling

  2012cites this paper
• Sphingolipid Δ8 unsaturation is important for glucosylceramide biosynthesis and low-temperature performance in Arabidopsis.

  2012influential citation
• New insights into cell death induced by long chain bases in Arabidopsis.

  2011cites this paper
• Isolation and characterization of fatty acid desaturase genes from peanut (Arachis hypogaea L.)

  2011cites this paper
• Phosphatidic Acid Binds and Stimulates Arabidopsis Sphingosine Kinases*

  2011cites this paper
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  2011cites this paper
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  2011cites this paper