Determination of collagen content within picrosirius red stained paraffin-embedded tissue sections using fluorescence microscopy

Graphical abstract

• Publication year

  2015

• Venue

  MethodsX

• Publication date

  2015-02-21

• Fields of study

  Biology, Medicine, Materials Science

• Identifiers
  DOI 10.1016/j.mex.2015.02.007PMID 26150980PMCID 4487704
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Toll4 (TLR4) expression in cardiac myocytes in normal and failing myocardium.

  1999influential reference
• Elimination of autofluorescence in immunofluorescence microscopy with digital image processing.

  1995cited by this paper
• Quantitative assessment of myocardial collagen with picrosirius red staining and circularly polarized light

  1994cited by this paper
• Lipofuscin-induced autofluorescence of living neonatal rat cardiomyocytes in culture.

  1994cited by this paper
• Conventional and confocal fluorescence microscopy of collagen fibers in the heart.

  1993cited by this paper
• Polarization microscopic studies of connective tissue stained with picro-sirius red FBA.

  1973cited by this paper

• N-(2-Hydroxyphenyl)-2-propylpentanamide Modulates HDAC1 and GPER1 Expression in a Rodent Model of Triple-Negative Breast Cancer.

  2026cites this paper
• Segment Regeneration of an Earthworm I: Formation of the Body Wall Tissues from Injury to Recovery

  2026cites this paper
• Neutrophil terminal programming in the ischemic heart drives fibrosis after myocardial infarction

  2026cites this paper
• Cell‐Surface LAMP1 is a Senescence Marker in Aging and Idiopathic Pulmonary Fibrosis

  2025cites this paper
• Transversalis fascia collagen content and the risk of surgical complications: results of a prospective study

  2025influential citation
• Map2k6 is a potent genetic modifier of arterial rupture in vascular Ehlers-Danlos syndrome mice

  2025cites this paper
• Cardiac Troponin I Antibodies Induce Cardiomyocyte Damage and Alter Cell Morphology

  2025cites this paper
• Loss of Na+,HCO3 −‐Cotransporter NBCn1 Inhibits Net Acid Extrusion in the Atria and Causes Hypertension‐Associated Cardiac Hypertrophy

  2025cites this paper
• Factors Affecting the Evaluation of Collagen Deposition and Fibrosis In Vitro

  2024cites this paper
• Characterizing the immune response to myocardial infarction in pigs

  2024cites this paper
• Assessment of the nano-mechanical properties of healthy and atherosclerotic coronary arteries by atomic force microscopy

  2024cites this paper
• Application of histochemical stains in anatomical research: A brief overview of the methods

  2024cites this paper
• A CNA-35-based high-throughput fibrosis assay reveals ORAI1 as a regulator of collagen release from pancreatic stellate cells.

  2024cites this paper
• Vascular inflammaging: Endothelial CEACAM1 expression is upregulated by TNF‐α via independent activation of NF‐κB and β‐catenin signaling

  2024cites this paper
• Central and peripheral mechanisms underlying respiratory deficits in a mouse model of accelerated senescence

  2024influential citation
• Robotic Ultrasound and Novel Collagen Analyses for Polycystic Kidney Disease Research Using Mice

  2024cites this paper
• "Melatonin protects against diclofenac induced oxidative stress mediated myocardial toxicity in rats: a mechanistic insight".

  2024cites this paper
• Cardiomyocyte-specific RXFP1 overexpression protects against pressure overload-induced cardiac dysfunction independently of Relaxin.

  2024cites this paper
• Macro-based collagen quantification and segmentation in picrosirius red-stained heart sections using light microscopy

  2024cites this paper
• Effects of Saline-Alkali Stress on Sugar Metabolism of Jujube Fruit: A Metabolomic Analysis

  2023cites this paper
• A comparison of confocal and epifluorescence microscopy for quantification of RNAScope and immunohistochemistry fluorescent images.

  2023cites this paper
• Krüppel-like Factor 5 Plays an Important Role in the Pathogenesis of Chronic Pancreatitis

  2023cites this paper
• Construct and criterion validity of muscle ultrasonography for assessment of skeletal muscle in patients recovering from COVID-19

  2023cites this paper
• Effect of sequential release of sirolimus and rosuvastatin using silk fibroin microneedle to prevent intimal hyperplasia.

  2023cites this paper
• Dietary supplementation of cystinotic mice by lysine inhibits the megalin pathway and decreases kidney cystine content

  2023cites this paper
• An mTORC1‐Dependent Mouse Model for Cardiac Sarcoidosis

  2023cites this paper
• LiverQuant: An Improved Method for Quantitative Analysis of Liver Pathology

  2023cites this paper
• Determination of Interstitial Collagen Deposition and Related Topography Using the Second Harmonic Generation-Based HistoIndex Platform.

  2023cites this paper
• Encapsulated papillary carcinoma of the breast: does it have a native basement membrane?

  2023cites this paper
• Ablation of C-type natriuretic peptide/cGMP signaling in fibroblasts exacerbates adverse cardiac remodeling in mice

  2023cites this paper
• Assessment of Collagen in Translational Models of Lung Research.

  2023cites this paper
• Combined transcriptomic and metabolomic analyses reveal the mechanism of debagged ‘Fuji’ apple sunburn

  2023cites this paper
• Desmosomal protein degradation as an underlying cause of arrhythmogenic cardiomyopathy

  2023cites this paper
• Mechanistic Insights of the LEMD2 p.L13R Mutation and Its Role in Cardiomyopathy

  2023cites this paper
• Wnt11 in regulation of physiological and pathological cardiac growth

  2022cites this paper
• Rapid histological quantification of muscle fibrosis and lysosomal activity using the HSB colour space

  2022cites this paper
• The direct effect of fibroblast growth factor 23 on vascular smooth muscle cell phenotype and function

  2022cites this paper
• Time-Dependent Collagen Fibered Structure in the Early Distraction Callus: Imaging Characterization and Mathematical Modeling

  2022cites this paper
• Altered type I collagen networking in osteoporotic human femoral head revealed by histomorphometric and Fourier transform infrared imaging correlated analyses

  2022cites this paper
• PM2.5 Exposure Lowers Mitochondrial Endurance During Cardiac Recovery in a Rat Model of Myocardial Infarction

  2022cites this paper
• Melatonin ameliorates chronic copper-induced lung injury

  2022cites this paper
• Lack of food intake during shift work alters the heart transcriptome and leads to cardiac tissue fibrosis and inflammation in rats

  2022cites this paper
• Sex-Dependent Responses to Maternal Exposure to PM2.5 in the Offspring

  2022cites this paper
• Dynamic dentin: A quantitative microscopic assessment of age and spatial changes to matrix architecture, peritubular dentin, and collagens types I and III.

  2022cites this paper
• Construction of Artificial Ovaries with Decellularized Porcine Scaffold and Its Elicited Immune Response after Xenotransplantation in Mice

  2022cites this paper
• Non-destructive, continuous monitoring of biochemical, mechanical, and structural maturation in engineered tissue

  2022cites this paper
• Comparative efficacy of levosimendan, ramipril, and sacubitril/valsartan in isoproterenol induced experimental heart failure: a hemodynamic and molecular approach.

  2022cites this paper
• Nano-material utilization in stem cells for regenerative medicine

  2022cites this paper
• Lack of food intake during shift work alters the heart transcriptome and leads to cardiac tissue fibrosis and inflammation in rats

  2021cites this paper
• In Vitro Liver Toxicity Testing of Chemicals: A Pragmatic Approach

  2021cites this paper
• FABP3 deficiency exacerbates metabolic derangement in cardiac hypertrophy via PPARα pathway

  2021cites this paper
• Differences in synovial fibrosis relative to range of motion in knee osteoarthritis patients

  2021cites this paper
• Solanum xanthocarpum fruit extract promotes chondrocyte proliferation in vitro and protects cartilage damage in collagenase induced osteoarthritic rats (Article reference number: JEP 114028).

  2021cites this paper
• A simple method for quantitating confocal fluorescent images

  2021cites this paper
• Chronic photoperiodic manipulation induced chronodisruption upregulates HSP60 during early pro-atherogenic remodeling in thoracic aorta of C57BL/6J mice

  2021cites this paper
• Concomitant control of mechanical properties and degradation in resorbable elastomer-like materials using stereochemistry and stoichiometry for soft tissue engineering

  2021cites this paper
• MiR-185-5p regulates the development of myocardial fibrosis.

  2021cites this paper
• Beyond muscles: Role of intramuscular connective tissue elasticity and passive stiffness in the octopus arm muscle function.

  2021cites this paper
• Boswellia serrata Extract Containing 30% 3-Acetyl-11-Keto-Boswellic Acid Attenuates Inflammatory Mediators and Preserves Extracellular Matrix in Collagen-Induced Arthritis

  2021cites this paper
• Architecture and Composition Dictate Viscoelastic Properties of Organ-Derived Extracellular Matrix Hydrogels

  2021cites this paper
• A tumor-derived type III collagen-rich ECM niche regulates tumor cell dormancy

  2021cites this paper
• New Mechanistic Insights to PLOD1-mediated Human Vascular Disease.

  2021cites this paper
• Nile tilapia skin (Oreochromis niloticus) for burn treatment: ultrastructural analysis and quantitative assessment of collagen.

  2021cites this paper
• 5-HTT Deficiency in Male Mice Affects Healing and Behavior after Myocardial Infarction

  2021cites this paper
• Suppression of Inflammatory Cardiac Cytokine Network in Rats with Untreated Obesity and Pre-Diabetes by AT2 Receptor Agonist NP-6A4

  2021cites this paper
• The healing myocardium mobilises a distinct B-cell subset through a CXCL13-CXCR5-dependent mechanism.

  2021cites this paper
• Nasal Chondrocyte–Based Engineered Grafts for the Repair of Articular Cartilage “Kissing” Lesions: A Pilot Large-Animal Study

  2021cites this paper
• Histology of human teeth: Standard and specific staining methods revisited.

  2021cites this paper
• HSP60 knockdown exerts differential response in endothelial cells and monocyte derived macrophages during atherogenic transformation

  2021cites this paper
• Immune Reconstitution and Thymic Involution in the Acute and Delayed Hematopoietic Radiation Syndromes

  2020cites this paper
• Accelerated liver recovery after acute CCl4 poisoning in rats treated with sodium phthalhydrazide.

  2020cites this paper
• Elastic, Persistently Moisture-Retentive and Wearable Biomimetic Film Inspired by Fetal Scarless Repair for Promoting Skin Wound Healing.

  2020cites this paper
• Aggregation-induced emission luminogen for specific identification of malignant tumour in vivo

  2020cites this paper
• Collagenase‐Cleavable Peptide Amphiphile Micelles as a Novel Theranostic Strategy in Atherosclerosis

  2020cites this paper
• Injectable Enzyme-Based Hydrogel Matrix with Precisely Oxidative Stress Defense for Promoting Dermal Repair of Burn Wound.

  2020cites this paper
• Systemic blockade of ACVR2B ligands attenuates muscle wasting in ischemic heart failure without compromising cardiac function

  2020cites this paper
• Impaired Gut–Systemic Signaling Drives Total Parenteral Nutrition-Associated Injury

  2020cites this paper
• Heparanase Promotes Syndecan-1 Expression to Mediate Fibrillar Collagen and Mammographic Density in Human Breast Tissue Cultured ex vivo

  2020cites this paper
• Reexploring picrosirius red: A review

  2020cites this paper
• Cardiac morphological and functional changes induced by C-type natriuretic peptide are different in normotensive and spontaneously hypertensive rats

  2020cites this paper
• Evidence of stage progression in a novel, validated fluorescence-navigated and microsurgical-assisted secondary lymphedema rodent model

  2020cites this paper
• Influence of Obesity on the Organization of the Extracellular Matrix and Satellite Cell Functions After Combined Muscle and Thorax Trauma in C57BL/6J Mice

  2020cites this paper
• Antiosteoarthritic effect of Punica granatum L. peel extract on collagenase induced osteoarthritis rat by modulation of COL‐2, MMP‐3, and COX‐2 expression

  2020cites this paper
• The heterogeneous morphology of networked collagen in distal colon and rectum of mice quantified via nonlinear microscopy.

  2020cites this paper
• PDE5 inhibition improves cardiac morphology and function in SHR by reducing NHE1 activity: Repurposing sildenafil for the treatment of hypertensive cardiac hypertrophy.

  2020cites this paper
• Comparison of the structural and ultrastructural characteristics of the female prostate between pregnant and non-pregnant plains viscacha (Lagostomus maximus).

  2020cites this paper
• Digital Image Analysis of Picrosirius Red Staining: A Robust Method for Multi-Organ Fibrosis Quantification and Characterization

  2020cites this paper
• An investigation into the critical role of fibre orientation in the ultimate tensile strength and stiffness of human carotid plaque caps

  2020cites this paper
• Extracellular matrix in ascending aortic aneurysms and dissections – What we learn from decellularization and scanning electron microscopy

  2019cites this paper
• Collagen Peptide Presenting Nanofibrous Scaffold for Intervertebral Disc Regeneration.

  2019cites this paper
• Molecular and structural insights into skin collagen reveals several factors that influence its architecture.

  2019cites this paper
• Transgenic Expression of miR-133a in the Diabetic Akita Heart Prevents Cardiac Remodeling and Cardiomyopathy

  2019cites this paper
• Evaluation of Collagen and Elastin Content in Skin of Multiparous Minks Receiving Feed Contaminated with Deoxynivalenol (DON, vomitoxin) with or without Bentonite Supplementation

  2019cites this paper
• Quantitative nanohistology : collagen disorders of connective and mineralised tissues

  2019cites this paper
• SPRED2 deficiency elicits cardiac arrhythmias and premature death via impaired autophagy.

  2019cites this paper
• ИММУНОМОДУЛИРУЮЩЕЕ ВЛИЯНИЕ АМИНОФТАЛГИДРАЗИДА НА КЛЕТКИ ПЕЧЕНИ ПРИ ДИФФУЗНОМ ТОКСИЧЕСКОМ ПОВРЕЖДЕНИИ

  2019cites this paper
• Hypothermic and cryogenic preservation of tissue‐engineered human bone

  2019cites this paper
• Targetable cellular signaling events mediate vascular pathology in vascular Ehlers-Danlos syndrome.

  2019cites this paper
• Interstitial fibroblasts in donor kidneys predict late posttransplant anemia

  2019cites this paper
• Strong Signs for a Weak Wall in Tricuspid Aortic Valve Associated Aneurysms and a Role for Osteopontin in Bicuspid Aortic Valve Associated Aneurysms

  2019cites this paper