Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations

No abstract is available for this paper.

• Publication year

  1990

• Venue

  Applied and Environmental Microbiology

• Publication date

  1990-06-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1128/aem.56.6.1919-1925.1990PMID 2200342PMCID PMC184531
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology

  1990influential reference
• Green photosynthetic bacteria associated with the deep chlorophyll maximum of the Sargasso sea

  1989cited by this paper
• Characterizing aquatic bacteria according to population, cell size, and apparent DNA content by flow cytometry.

  1989cited by this paper
• Natural relationships among sulfate-reducing eubacteria

  1989cited by this paper
• Phylogenetic stains: ribosomal RNA-based probes for the identification of single cells.

  1989cited by this paper
• Shifts in Methanogenic Subpopulations Measured with Antibody Probes in a Fixed-Bed Loop Anaerobic Bioreactor Treating Sulfite Evaporator Condensate

  1989cited by this paper
• Conversion of logarithmic channel numbers into relative linear fluorescence intensity.

  1988cited by this paper
• Flow cytometric detection of ribosomal RNA in suspended cells by fluorescent in situ hybridization.

  1988cited by this paper
• Use of phylogenetically based hybridization probes for studies of ruminal microbial ecology

  1988cited by this paper
• Vertical distribution of North Atlantic ultraphytoplankton: analysis by flow cytometry and epifluorescence microscopy

  1988cited by this paper
• Analysis of Synechococcus pigment types in the sea using single and dual beam flow cytometry

  1988cited by this paper
• Phylogenetic group-specific oligodeoxynucleotide probes for identification of single microbial cells

  1988cited by this paper
• Bacterial evolution

  1987cited by this paper
• Microbial ecology and evolution: a ribosomal RNA approach.

  1986cited by this paper
• Respiratory Quinones of Sulphate-Reducing and Sulphur-Reducing Bacteria: A Systematic Investigation

  1986cited by this paper
• Phylogenetic Relationships Among the Sulfate Respiring Bacteria, Myxobacteria and Purple Bacteria

  1985cited by this paper
• Marine Ammonia- and Nitrite-Oxidizing Bacteria: Serological Diversity Determined by Immunofluorescence in Culture and in the Environment

  1985cited by this paper
• Natural relationship between bacteroides and flavobacteria

  1985cited by this paper
• Sequence of the 16S Ribosomal RNA Gene from Methanococcus vannielii: Evolutionary Implications

  1985cited by this paper
• The phylogeny of archaebacteria

  1985cited by this paper
• A phylogenetic definition of the major eubacterial taxa.

  1985cited by this paper
• Nucleotide sequence of the Bacillus subtilis ribosomal RNA operon, rrnB.

  1985cited by this paper
• Identification and Enumeration of Marine Chroococcoid Cyanobacteria by Immunofluorescence

  1983cited by this paper
• Nucleotide sequence of the Dictyostelium discoideum small-subunit ribosomal ribonucleic acid inferred from the gene sequence: evolutionary implications

  1983cited by this paper
• The use of DAPI for identifying and counting aquatic microflora1

  1980cited by this paper
• The Immunofluorescence Approach in Microbial Ecology

  1980cited by this paper
• Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli.

  1978cited by this paper
• Use of nuclepore filters for counting bacteria by fluorescence microscopy

  1977cited by this paper
• Control of Macromolecular Synthesis

  1966cited by this paper

• First report of Aquitalea spp. From cave aquatic environments of Roraima Tepui, Guayana highlands

  2026cites this paper
• BEEP Learning: Multi-View Image Decomposition for Massively Multiplexed Biological Fluorescence Microscopy

  2026cites this paper
• A tumor-resident bacterium promotes cervical cancer progression through lysoPC-mediated c-Jun/c-Fos activation.

  2026cites this paper
• Anaerobic methane oxidation by ANME-2a at two molar chloride in Orca Basin

  2026cites this paper
• Setting a polyphasic approach to study Antarctic cryptoendolithic communities as a toolkit for astrobiology

  2026cites this paper
• Bacteroides intestinalis-Driven Arabinoxylan Fermentation Mitigates Inflammatory and Metabolic Dysfunction

  2026cites this paper
• The discovery of an external bacterial niche reconciles sequencing-based microbiomes in a freshwater sponge

  2026cites this paper
• Single-cell mass accumulation reveals bacterioplankton growth rate in native seawater

  2025cites this paper
• Symbiosis and horizontal gene transfer promote herbivory in the megadiverse leaf beetles.

  2025cites this paper
• Oral Microbiome in Periodontal Disease: Where Are We Now?

  2025cites this paper
• Minisyncoccus archaeiphilus gen. nov., sp. nov., a mesophilic, obligate parasitic bacterium and proposal of Minisyncoccaceae fam. nov., Minisyncoccales ord. nov., Minisyncoccia class. nov. and Minisyncoccota phyl. nov. formerly referred to as Candidatus Patescibacteria or candidate phyla radiation

  2025cites this paper
• Multifunctional endophytic bacteria intimately associated within spores of arbuscular mycorrhizal fungi in a chernozem soil in Central Europe

  2025cites this paper
• A collection of archaeal 16S rRNA Clone-FISH cultures for probe validation in fluorescence in situ hybridization experiments

  2025cites this paper
• High-temporal resolution of microbial food web dynamics and structure during phytoplankton blooms in the Baltic Sea

  2025cites this paper
• Shining Light on Oral Biofilm Fluorescence In Situ Hybridization (FISH): Probing the Accuracy of In Situ Biogeography Studies

  2025influential citation
• Elucidating the factors that impact the abundance of aerobic and denitrifying polyphosphate‐accumulating organisms in an integrated fixed film activated sludge‐enhanced biological phosphorus removal process

  2025cites this paper
• Genomic and metabolic characterisation of a novel species Magnetominusculus dajiuhuensis DJH-1Ts sp. nov. from an acidic peatland.

  2025cites this paper
• Metagenomic analysis’ rhizomicrobiome’ Prunus mahaleb based on 16S RNA gene sequencing on the Illumina MiSeq

  2025cites this paper
• Newly Designed Fluorescence In Situ Hybridization Probes Reveal Previously Unknown Endophytic Abilities of Tuber magnatum in Herbaceous Plants

  2025cites this paper
• Structure of fungal community and culturable fungi on the discolored surfaces of pine storage boxes in the tropical region in Dong Nai, Vietnam.

  2025cites this paper
• Denitrifying Dephosphatation via Nitrite under Anoxic Conditions

  2025cites this paper
• Nitrosarchaeum haohaiensis sp. Nov. CL1T : Isolation and Characterisation of a Novel Ammonia‐Oxidising Archaeon From Aquatic Environments

  2025cites this paper
• Exploring the feasibility of high rate enhanced biological phosphorus removal system driven by diverse carbon source

  2025cites this paper
• Highly multiplexed spatial transcriptomics in bacteria.

  2025cites this paper
• Addition of microbial consortium to the rice straw biomethanization: effect on specific methanogenic activity, kinetic and bacterial community

  2025cites this paper
• Obtaining complete and canonical ammonia-oxidizing bacteria through specific labeling and cell sorting

  2025cites this paper
• Self-Immolative Arylcarbonates for Nucleic Acid Templated Reactions.

  2025cites this paper
• Laser-assisted microbial culturomics

  2025cites this paper
• Lactobacillus helveticus R0052 and Bifidobacterium longum R0175 Administration to Long-Evans Rats Has Interactive Effects with Sex and Diet on Anxiety-Related Feeding Behaviors and Specific Endocrine Outcomes.

  2025cites this paper
• Intracellular Localization of the Bacterial Endosymbiont Cardinium in the Ostracod Heterocypris spadix.

  2025cites this paper
• Protocol to investigate gene expression heterogeneity in cyanobacteria using mRNA CARD-FISH

  2025cites this paper
• Slicing overcomes the bacterial cell wall barrier to fluorescence in situ hybridization

  2025cites this paper
• Annual changes in the microeukaryotic community in intertidal sediments, Qingdao, China

  2025cites this paper
• Microbiome spatial scaling varies among members, hosts, and environments across model island ecosystems

  2025cites this paper
• Vertical transmission of Cardinium bacteria in parthenogenetic non-marine ostracods (Crustacea).

  2025cites this paper
• Urea use drives niche separation between dominant marine ammonia oxidizing archaea

  2025cites this paper
• Abundance of bacteria and histopathologic findings in the small intestinal mucosa of dogs with chronic inflammatory enteropathies

  2025cites this paper
• OligoN-design: A simple and versatile tool to design specific probes and primers from large heterogeneous datasets

  2025cites this paper
• The contribution of pelagic fungi to ocean biomass.

  2025influential citation
• Vertical transfer of bacterial symbionts via a placental analogue in the cyclostome bryozoan Patinella verrucaria (Stenolaemata): Ultrastructural and molecular evidence.

  2025cites this paper
• Epidemiology of bacterial biofilms on polyps and normal tissues in a screening colonoscopy cohort

  2025cites this paper
• The quest for environmental analytical microbiology: absolute quantitative microbiome using cellular internal standards

  2025cites this paper
• Immobilization and Monitoring of Clostridium carboxidivorans and Clostridium kluyveri in Synthetic Biofilms

  2025cites this paper
• Molecular piracy in deep-sea hydrothermal vent: phage-plasmid interactions revealed by phage-FISH in Marinitoga piezophila

  2025influential citation
• Bacterial colonization contributes to pathological scar formation via the regulation of inflammatory response

  2025cites this paper
• Far‐red light acclimation in Phaeophila dendroides (Ulvales, Ulvophyceae) isolated from a coral skeleton

  2025cites this paper
• Pseudoalteromonas is a novel symbiont of marine invertebrates that exhibits broad patterns of phylosymbiosis

  2025cites this paper
• Uncultivable and cultivable gut symbiotic bacteria of the jewel stinkbug Scutellera amethystina (Hemiptera: Scutelleridae)

  2025influential citation
• Can infectious omphalitis in piglets be clinically diagnosed during the first three days of life?

  2025cites this paper
• Selective pressure of various levels of erythromycin on the development of antibiotic resistance.

  2025cites this paper
• Environmental diversity of Candidatus Babelota and their relationships with protists

  2025cites this paper
• In situ imaging and microbiome analysis of calculus‐like deposits at the root apex: A case report of refractory apical periodontitis

  2025cites this paper
• Applicability of live-fluorescence in situ hybridization (Live-FISH) on soil microbiomes

  2025cites this paper
• Paenibacillus zeirhizosphaerae sp. nov., isolated from surface of the maize (Zea mays) roots in a horticulture field, Hungary.

  2025cites this paper
• Single-cell quantification of the microbiota by flow cytometry: MicFLY

  2025cites this paper
• Biofilm-associated Achromobacter xylosoxidans prosthetic valve infective endocarditis.

  2025cites this paper
• Development of a High-Rate Biological System for the Complete Treatment (Nitrification) of Low Strength Wastewater

  2025cites this paper
• Legionella Survives and Elongates in Algal Consortia Containing Bacteria in Alkaline Oligotrophic Conditions

  2025cites this paper
• Complete and stable enhanced biological phosphorus removal at 30 °C using glutamate and acetate as alternating carbon sources.

  2025cites this paper
• GenomeFISH: genome-based fluorescence in situ hybridization for strain-level visualization of microbial communities

  2025cites this paper
• Identification of a surface protein in the extracellular polymeric substances of seawater-adapted aerobic granular sludge

  2025cites this paper
• Bacterial Dynamics in Newly Settled Acropora kenti: Insights From Inoculations With Individual Probiotic Candidates

  2025cites this paper
• Recruiting high-efficiency denitrifying consortia using Pseudomonas aeruginosa.

  2025cites this paper
• Amino acid competition shapes Acinetobacter baumannii gut carriage

  2025cites this paper
• Co-substrate utilisation in "Candidatus Accumulibacter" enhances metabolic fitness in dynamic environments.

  2025cites this paper
• Distinct localization patterns of Treponematales (Spirochaetota) lineages in the gut microbiome of Coptotermes formosanus

  2025cites this paper
• Sequence-Structure Combination Strategy Enables Discovery of a Novel Transglutaminase from Krasilnikovia cinnamomea and Computational Analysis.

  2025cites this paper
• Globally occurring pelagiphage infections create ribosome-deprived cells

  2024cites this paper
• Blood-borne immune cells carry low biomass DNA remnants of microbes in patients with colorectal cancer or inflammatory bowel disease

  2024cites this paper
• ANTIMICROBIAL ACTIVITY OF SPECIALISED METABOLITES OF SOIL STREPTOMYCETES-CHITINOLYTIC

  2024cites this paper
• Coral-infecting parasites in cold marine ecosystems.

  2024cites this paper
• Association of Acidotolerant Cyanobacteria to Microbial Mats below pH 1 in Acidic Mineral Precipitates in Río Tinto River in Spain

  2024cites this paper
• Zoothamnium mariella sp. nov., a marine, colonial ciliate with an atypcial growth pattern, and its ectosymbiont Candidatus Fusimicrobium zoothamnicola gen. nov., sp. nov.

  2024cites this paper
• Extracellular host DNA contributes to pathogenic biofilm formation during periodontitis

  2024cites this paper
• Mechanisms of extracellular electron transfer in anaerobic methanotrophic archaea

  2024cites this paper
• The Microbiome Modulates the Immune System to Influence Cancer Therapy

  2024cites this paper
• CRB1-associated retinal degeneration is dependent on bacterial translocation from the gut.

  2024cites this paper
• Comparison of Prebiotic Candidates in Ulcerative Colitis using an in vitro Fermentation Model.

  2024cites this paper
• Continental scientific drilling and microbiology: (extremely) low biomass in bedrock of central Sweden

  2024cites this paper
• MUC17 is an essential small intestinal glycocalyx component that is disrupted in Crohn’s disease

  2024cites this paper
• Genome concentration limits cell growth and modulates proteome composition in Escherichia coli

  2024cites this paper
• Methanobrevibacter massiliense and Pyramidobacter piscolens Co-Culture Illustrates Transkingdom Symbiosis

  2024cites this paper
• Anionic extracellular polymeric substances extracted from seawater-adapted aerobic granular sludge

  2024cites this paper
• Biomass attachment and microbiota shifts during porcine faecal in vitro fermentation of almond and macadamia nuts differing in particle sizes.

  2024cites this paper
• Precision enzyme discovery through targeted mining of metagenomic data

  2024cites this paper
• Late-stage borreliosis and substance abuse

  2024cites this paper
• Fecal-adherent mucus is a non-invasive source of primary human MUC2 for structural and functional characterization in health and disease

  2024cites this paper
• Rapid and Accurate Quantification of Viable Lactobacillus Cells in Infant Formula by Flow Cytometry Combined with Propidium Monoazide and Signal-Enhanced Fluorescence In Situ Hybridization.

  2024cites this paper
• Ecology of Legionella pneumophila biofilms: The link between transcriptional activity and the biphasic cycle

  2024cites this paper
• Organ structure and bacterial microbiogeography in a reproductive organ of the Hawaiian bobtail squid reveal dimensions of a defensive symbiosis

  2024cites this paper
• Association of intratumoral microbiome diversity with hepatocellular carcinoma prognosis

  2024cites this paper
• Specificity of benthic invertebrate gill-associated microbiome contributes to host fitness to localized heterogeneous environment in the cold seep.

  2024cites this paper
• Diversity and physiology of abundant Rhodoferax species in global wastewater treatment systems.

  2024cites this paper
• Symbiont Acquisition Strategies in Post‐Settlement Stages of Two Co‐Occurring Deep‐Sea Rimicaris Shrimp

  2024cites this paper
• Exploring interspecific interaction variability in microbiota: A review

  2024cites this paper
• The adaptation of chlamydiae to facultative host multicellularity

  2024cites this paper
• Intratumoral microbiota as cancer therapeutic target

  2024cites this paper
• Prevotella timonensis degrades the vaginal epithelial glycocalyx through high fucosidase and sialidase activities

  2024cites this paper
• Stable isotopes and nanoSIMS single-cell imaging reveals soil plastisphere colonizers able to assimilate sulfamethoxazole

  2024influential citation
• High accuracy meets high throughput for near full-length 16S ribosomal RNA amplicon sequencing on the Nanopore platform

  2024cites this paper