Monitoring metabolites consumption and secretion in cultured cells using ultra-performance liquid chromatography quadrupole–time of flight mass spectrometry (UPLC–Q–ToF-MS)

No abstract is available for this paper.

• Publication year

  2011

• Venue

  Analytical and Bioanalytical Chemistry

• Publication date

  Unknown publication date

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1007/s00216-011-5556-4PMID 22159369
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Metabolome in schizophrenia and other psychotic disorders: a general population-based study

  2011cited by this paper
• Systems level studies of mammalian metabolomes: the roles of mass spectrometry and nuclear magnetic resonance spectroscopy.

  2011cited by this paper
• Metabolomic analysis via reversed-phase ion-pairing liquid chromatography coupled to a stand alone orbitrap mass spectrometer.

  2010cited by this paper
• A plasma signature of human mitochondrial disease revealed through metabolic profiling of spent media from cultured muscle cells

  2010cited by this paper
• Orotic acid quantification in dried blood spots and biological fluids by hydrophilic interaction liquid chromatography tandem mass spectrometry.

  2010cited by this paper
• Ultrahigh performance liquid chromatography-tandem mass spectrometry method for fast and robust quantification of anionic and aromatic metabolites.

  2010cited by this paper
• CE-MS for metabolic profiling of volume-limited urine samples: application to accelerated aging TTD mice.

  2010cited by this paper
• Highly sensitive matrix-assisted laser desorption ionization-mass spectrometry for high-throughput metabolic profiling.

  2010cited by this paper
• A protocol for generating a high-quality genome-scale metabolic reconstruction

  2010cited by this paper
• Metabolic profiling of ultrasmall sample volumes with GC/MS: from microliter to nanoliter samples.

  2010cited by this paper
• Development of a robust and repeatable UPLC-MS method for the long-term metabolomic study of human serum.

  2009cited by this paper
• Absolute Metabolite Concentrations and Implied Enzyme Active Site Occupancy in Escherichia coli

  2009cited by this paper
• Connecting extracellular metabolomic measurements to intracellular flux states in yeast

  2009cited by this paper
• Metabolomic Profiling of Drug Responses in Acute Myeloid Leukaemia Cell Lines

  2009cited by this paper
• Analysis of highly polar metabolites in human plasma by ultra-performance hydrophilic interaction liquid chromatography coupled with quadrupole-time of flight mass spectrometry.

  2009cited by this paper
• Blunting of AICAR-induced human skeletal muscle glucose uptake in type 2 diabetes is dependent on age rather than diabetic status

  2009cited by this paper
• Multiple ionization mass spectrometry strategy used to reveal the complexity of metabolomics.

  2008cited by this paper
• Mass spectrometry-based metabolomics: accelerating the characterization of discriminating signals by combining statistical correlations and ultrahigh resolution.

  2008cited by this paper
• Regulation of ERK1/2 by ouabain and Na-K-ATPase-dependent energy utilization and AMPK activation in parotid acinar cells.

  2008cited by this paper
• Current trends and future requirements for the mass spectrometric investigation of microbial, mammalian and plant metabolomes

  2008cited by this paper
• Evaluation of mobile phase, ion pairing, and temperature influence on an HILIC-MS/MS method for L-arginine and its dimethylated derivatives detection.

  2008cited by this paper
• Global metabolic profiling analysis on human urine by UPLC-TOFMS: issues and method validation in nutritional metabolomics.

  2008cited by this paper
• Global reconstruction of the human metabolic network based on genomic and bibliomic data

  2007cited by this paper
• Protein kinase A phosphorylation of the multifunctional protein CAD antagonizes activation by the MAP kinase cascade

  2007cited by this paper
• New uses for old drugs

  2007cited by this paper
• Within-day reproducibility of an HPLC-MS-based method for metabonomic analysis: application to human urine.

  2007cited by this paper
• NMR-based metabolomics: translational application and treatment of cancer.

  2007cited by this paper
• A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information

  2007cited by this paper
• Biomarkers for NeuroAIDS: The Widening Scope of Metabolomics

  2007cited by this paper
• Towards multidimensional genome annotation

  2006cited by this paper
• Metabolomics-edited transcriptomics analysis of Se anticancer action in human lung cancer cells

  2006cited by this paper
• Systems Biology: Properties of Reconstructed Networks

  2006cited by this paper
• Assessing responses to cancer therapy using molecular imaging.

  2006cited by this paper
• Closed-loop, multiobjective optimization of analytical instrumentation: gas chromatography/time-of-flight mass spectrometry of the metabolomes of human serum and of yeast fermentations.

  2005cited by this paper
• Investigation of retention on bare silica using reversed-phase mobile phases at elevated temperatures.

  2004cited by this paper
• Reconstruction and validation of Saccharomyces cerevisiae iND750, a fully compartmentalized genome-scale metabolic model.

  2004cited by this paper
• Bioanalytical liquid chromatography tandem mass spectrometry methods on underivatized silica columns with aqueous/organic mobile phases.

  2003cited by this paper
• Roles of 5'-AMP-activated protein kinase (AMPK) in mammalian glucose homoeostasis.

  2003cited by this paper
• High-throughput classification of yeast mutants for functional genomics using metabolic footprinting

  2003cited by this paper
• Cellular environment of metabolites and a metabonomic study of tamoxifen in endometrial cells using gradient high resolution magic angle spinning 1H NMR spectroscopy.

  2003cited by this paper
• Combining Genomics, Metabolome Analysis, and Biochemical Modelling to Understand Metabolic Networks

  2001cited by this paper
• Role of AMP-activated protein kinase in mechanism of metformin action.

  2001cited by this paper
• Activation of glycogen phosphorylase and glycogenolysis in rat skeletal muscle by AICAR — an activator of AMP‐activated protein kinase

  1996cited by this paper
• 5-aminoimidazole-4-carboxamide ribonucleoside. A specific method for activating AMP-activated protein kinase in intact cells?

  1995cited by this paper

• Bacterial pathogen deploys the iminosugar glycosyrin to manipulate plant glycobiology.

  2025cites this paper
• Temozolomide-Derived AIC Is Incorporated into Purine Synthesis in Glioblastoma

  2025cites this paper
• Plant seedlings of peas, tomatoes, and cucumbers exude compounds that are needed for growth and chemoattraction of Rhizobium leguminosarum bv. viciae 3841 and Azospirillum brasilense Sp7.

  2024cites this paper
• Comprehensive Coverage of Glycolysis and Pentose Phosphate Metabolic Pathways by Isomer-Selective Accurate Targeted Hydrophilic Interaction Liquid Chromatography-Tandem Mass Spectrometry Assay.

  2024cites this paper
• Cell Metabolomics Study on Synergistic anti-Hepatocellular Carcinoma Effect of Aidi Injection Combined with Doxorubicin.

  2022cites this paper
• Metabolic Response in Endothelial Cells to Catecholamine Stimulation Associated with Increased Vascular Permeability

  2022cites this paper
• COVID-19 Rehabilitation With Herbal Medicine and Cardiorespiratory Exercise: Protocol for a Clinical Study

  2021cites this paper
• Microbial production of small peptide: pathway engineering and synthetic biology

  2021cites this paper
• Toxicometabolomics: Small Molecules to Answer Big Toxicological Questions

  2021cites this paper
• A High-Throughput HILIC-MS-Based Metabolomic Assay for the Analysis of Polar Metabolites.

  2021cites this paper
• Mechanistic model-driven exometabolomic characterisation of human dopaminergic neuronal metabolism

  2021cites this paper
• Hydrophilic interaction liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry for determination of nuclear and cytoplasmatic contents of nucleotides, nucleosides and their nucleobases in food yeasts

  2021cites this paper
• Ion mobility mass spectrometry in the omics era: Challenges and opportunities for metabolomics and lipidomics.

  2021cites this paper
• Mass spectrometry-based quantification and spatial location of small organic acids exudate in plant roots under phosphorous deficiency and aluminium toxicity.

  2021cites this paper
• AICAr, a Widely Used AMPK Activator with Important AMPK-Independent Effects: A Systematic Review

  2021cites this paper
• A novel expressed prostatic secretion (EPS)-urine metabolomic signature for the diagnosis of clinically significant prostate cancer

  2021cites this paper
• Metabolic and innate immune cues merge into a specific inflammatory response via unfolded protein response (UPR)

  2020cites this paper
• COVID-19 Rehabilitation With Herbal Medicine and Cardiorespiratory Exercise: Protocol for a Clinical Study (Preprint)

  2020cites this paper
• Fructose Metabolism Contributes to the Warburg effect

  2020cites this paper
• Non-dietary Fructose Metabolism Contributes to the Warburg effect in Cancers

  2020cites this paper
• Development of a prototype device for near real-time surface-enhanced Raman scattering monitoring of biological samples.

  2020cites this paper
• Fructose fuels tumor growth through the polyol pathway and GLUT 8 transporter

  2020cites this paper
• Metabolomics applied to cultured human and animal cells

  2020cites this paper
• Metabolic and Innate Immune Cues Merge into a Specific Inflammatory Response via the UPR.

  2019cites this paper
• Metabolic programming determines the lineage-differentiation fate of murine bone marrow stromal progenitor cells

  2019cites this paper
• The ribonucleoside AICAr induces differentiation of myeloid leukemia by activating the ATR/Chk1 via pyrimidine depletion

  2019cites this paper
• Derailing the aspartate pathway of Mycobacterium tuberculosis to eradicate persistent infection

  2019cites this paper
• Hydrophilic interaction chromatography for the analysis of biopharmaceutical drugs and therapeutic peptides: A review based on the separation characteristics of the hydrophilic interaction chromatography phases.

  2019cites this paper
• Machine Learning Methods for Analysis of Metabolic Data and Metabolic Pathway Modeling

  2018cites this paper
• Fast and accurate quantitative organic acid analysis with LC-QTOF/MS facilitates screening of patients for inborn errors of metabolism

  2018cites this paper
• A Consensus Genome-scale Reconstruction of Chinese Hamster Ovary ( CHO ) Cell Metabolism

  2018influential citation
• Quantitative Analysis of Central Energy Metabolism in Cell Culture Samples.

  2018cites this paper
• Untargeted Metabolomics Reveal Lipid Alterations upon 2-Deoxyglucose Treatment in Human HaCaT Keratinocytes.

  2018cites this paper
• LC‐MS analysis of key components of the glutathione cycle in tissues and body fluids from mice with myocardial infarction

  2018cites this paper
• Quantitative NMR analysis of intra- and extracellular metabolism of mammalian cells: A tutorial.

  2017cites this paper
• Germline BAP1 mutations induce a Warburg effect

  2017cites this paper
• The Capacity of Mycobacterium tuberculosis To Survive Iron Starvation Might Enable It To Persist in Iron-Deprived Microenvironments of Human Granulomas

  2017cites this paper
• Time-Resolved Pharmacological Studies using Automated, On-line Monitoring of Five Parallel Suspension Cultures

  2017cites this paper
• Pre-analytic evaluation of volumetric absorptive microsampling and integration in a mass spectrometry-based metabolomics workflow

  2017cites this paper
• Collection and Preparation of Clinical Samples for Metabolomics.

  2017cites this paper
• Comprehensive optimization of LC–MS metabolomics methods using design of experiments (COLMeD)

  2016cites this paper
• Photoperiod Affects the Phenotype of Mitochondrial Complex I Mutants1[OPEN]

  2016cites this paper
• Unbiased Metabolomic Investigation of Alzheimer's Disease Brain Points to Dysregulation of Mitochondrial Aspartate Metabolism.

  2016cites this paper
• Dextromethorphan and its matabolites in rat bone tissues by gas chromatography-mass spectrometry analysis following differential microclimate decomposition

  2016cites this paper
• Liquid chromatography–high-resolution mass spectrometry-based cell metabolomics: Experimental design, recommendations, and applications

  2016cites this paper
• Extending metabolome coverage for untargeted metabolite profiling of adherent cultured hepatic cells

  2016cites this paper
• Targeted cofactor quantification in metabolically engineered E. coli using solid phase extraction and hydrophilic interaction liquid chromatography-mass spectrometry.

  2016cites this paper
• A Consensus Genome-scale Reconstruction of Chinese Hamster Ovary Cell Metabolism.

  2016cites this paper
• MetaboTools: A Comprehensive Toolbox for Analysis of Genome-Scale Metabolic Models

  2016cites this paper
• HILIC-MS for metabolomics: An attractive and complementary approach to RPLC-MS.

  2016cites this paper
• Metabolomics Analysis Reveals that AICAR Affects Glycerolipid, Ceramide and Nucleotide Synthesis Pathways in INS-1 Cells

  2015cites this paper
• Constraint-based modeling of host-microbe and microbe-microbe metabolic interactions in the human gut

  2015cites this paper
• Development and validation of an ultra-performance liquid chromatography quadrupole time of flight mass spectrometry method for rapid quantification of free amino acids in human urine

  2015cites this paper
• A simple coculture system shows mutualism between anaerobic faecalibacteria and epithelial Caco-2 cells

  2015cites this paper
• Extraction parameters for metabolomics from cultured cells.

  2015cites this paper
• Influence of HEK293 metabolism on the production of viral vectors and vaccine.

  2015cites this paper
• Metabolomic analysis of platelets during storage: a comparison between apheresis‐ and buffy coat–derived platelet concentrates

  2015cites this paper
• Development and validation of a HILIC‐MS/MS multitargeted method for metabolomics applications

  2015cites this paper
• Analytical methodology for metabolomics study of adherent mammalian cells using NMR, GC-MS and LC-HRMS

  2015cites this paper
• Extraction parameters for metabolomics from cell extracts

  2015cites this paper
• Reconstruction and systems analysis of metabolism in apicomplexan parasites Toxoplasma gondii and Plasmodium falciparum

  2015cites this paper
• Application and appraisal procedure of the vitamin B1 in egg nutrient value is assessed

  2015cites this paper
• Interaction between the gut and its microbiota in inflammatory bowel disease

  2015cites this paper
• The p60 and NamA autolysins from Listeria monocytogenes contribute to host colonization and induction of protective memory

  2015cites this paper
• Prediction of intracellular metabolic states from extracellular metabolomic data

  2014influential citation
• Analysis and pharmacokinetic study of curdione in Rhizoma Curcumae by UPLC/QTOF/MS.

  2014cites this paper
• Hydrophilic interaction chromatography of polar and ionizable compounds by UHPLC

  2014cites this paper
• UNIVERSIDADE FEDERAL DO RIO GRANDE DO NORTE CENTRO DE CIÊNCIAS DA SAÚDE PROGRAMA DE PÓS-GRADUAÇÃO EM CIÊNCIAS FARMACÊUTICAS DESENVOLVIMENTO E VALIDAÇÃO DE MÉTODOS ANALÍTICOS PARA DETERMINAÇÃO DE DIFERENTES GUANILHIDRAZONAS

  2014cites this paper
• Ion Mobility Derived Collision Cross Sections to Support Metabolomics Applications

  2014cites this paper
• Hydrophilic interaction ultra-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (HILIC-UPLC-TQ-MS/MS) in multiple-reaction monitoring (MRM) for the determination of nucleobases and nucleosides in ginkgo seeds.

  2014cites this paper
• Metabolomics in hypertension.

  2014cites this paper
• Comprehensive metabolomic study of platelets reveals the expression of discrete metabolic phenotypes during storage

  2014cites this paper
• Succinate Dehydrogenase is the Regulator of Respiration in Mycobacterium tuberculosis

  2014cites this paper
• Functional Metabolic Map of Faecalibacterium prausnitzii, a Beneficial Human Gut Microbe

  2014influential citation
• Ultra high performance liquid chromatography as a tool for the discovery and the analysis of biomarkers of diseases: a review.

  2013cites this paper
• Equipment and metabolite identification (ID) strategies for mass-based metabolomic analysis

  2013cites this paper
• Metabolomics of colorectal cancer: past and current analytical platforms

  2013cites this paper
• A community-driven global reconstruction of human metabolism

  2013cites this paper
• Metabolomics Study of Resina Draconis on Myocardial Ischemia Rats Using Ultraperformance Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry Combined with Pattern Recognition Methods and Metabolic Pathway Analysis

  2013cites this paper
• A marriage of two “Methusalem” drugs for the treatment of psoriasis?

  2013cites this paper
• Cell Metabolomics

  2013cites this paper
• Functional genomics of Plasmodium falciparum using metabolic modelling and analysis

  2013cites this paper
• Mammalian cell metabolomics: Experimental design and sample preparation

  2013cites this paper
• Hydrophilic interaction ultra-high performance liquid chromatography coupled with triple quadrupole mass spectrometry for determination of nucleotides, nucleosides and nucleobases in Ziziphus plants.

  2013cites this paper
• The importance of experimental design and QC samples in large-scale and MS-driven untargeted metabolomic studies of humans.

  2012cites this paper
• Intracellular metabolite profiling of platelets: evaluation of extraction processes and chromatographic strategies.

  2012cites this paper
• Separation Technique for the Determination of Highly Polar Metabolites in Biological Samples

  2012cites this paper
• Analytical method development in liquid chromatography-mass spectrometry based metabolomics

  year unknowncites this paper