An essential role for the C-terminal domain of a dragline spider silk protein in directing fiber formation.

No abstract is available for this paper.

• Publication year

  2006

• Venue

  Biomacromolecules

• Publication date

  2006-05-13

• Fields of study

  Biology, Medicine, Materials Science, Chemistry

• Identifiers
  DOI 10.1021/BM060120KPMID 16768399
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• The conserved C-termini contribute to the properties of spider silk fibroins.

  2005cited by this paper
• Wet spinning of Bombyx mori silk fibroin dissolved in N-methyl morpholine N-oxide and properties of regenerated fibres.

  2005cited by this paper
• Thermally induced alpha-helix to beta-sheet transition in regenerated silk fibers and films.

  2005cited by this paper
• Analysis of the conserved N-terminal domains in major ampullate spider silk proteins.

  2005cited by this paper
• Spider Silk Proteins – Mechanical Property and Gene Sequence

  2005cited by this paper
• Mapping domain structures in silks from insects and spiders related to protein assembly.

  2004cited by this paper
• Conserved C-termini of Spidroins are secreted by the major ampullate glands and retained in the silk thread.

  2004cited by this paper
• Novel assembly properties of recombinant spider dragline silk proteins.

  2004cited by this paper
• Structural characterization and artificial fiber formation of Bombyx mori silk fibroin in hexafluoro‐iso‐propanol solvent system

  2003cited by this paper
• Mechanism of silk processing in insects and spiders

  2003cited by this paper
• The molecular structure of spider dragline silk: Folding and orientation of the protein backbone

  2002cited by this paper
• Comparative architecture of silks, fibrous proteins and their encoding genes in insects and spiders.

  2002cited by this paper
• Liquid crystalline spinning of spider silk

  2001cited by this paper
• Synthetic spider silk: a modular fiber.

  2000cited by this paper
• Molecular biology of spider silk.

  2000cited by this paper
• Biology of spider silk.

  1999cited by this paper
• Swelling and dissolution of silk fibroin (Bombyx mori) in N-methyl morpholine N-oxide.

  1999cited by this paper
• The mechanical design of spider silks: from fibroin sequence to mechanical function.

  1999cited by this paper
• Hypotheses that correlate the sequence, structure, and mechanical properties of spider silk proteins.

  1999cited by this paper
• Molecular Orientation and Two-Component Nature of the Crystalline Fraction of Spider Dragline Silk

  1996cited by this paper
• Silk Properties Determined by Gland-Specific Expression of a Spider Fibroin Gene Family

  1996cited by this paper
• Sequence conservation in the C-terminal region of spider silk proteins (Spidroin) from Nephila clavipes (Tetragnathidae) and Araneus bicentenarius (Araneidae).

  1994cited by this paper
• Isolation of a clone encoding a second dragline silk fibroin. Nephila clavipes dragline silk is a two-protein fiber.

  1992cited by this paper
• Structure of a protein superfiber: spider dragline silk.

  1990cited by this paper
• Translational pauses during a spider fibroin synthesis.

  1983cited by this paper
• Comparative studies of fibroins. II. The crystal structures of various fibroins.

  1960cited by this paper

• Exploring the self-assembly of silk proteins through liquid-liquid phase separation

  2025cites this paper
• Negative stain TEM imaging of native spider silk protein superstructures.

  2025cites this paper
• Distinct Dimerization Mechanisms in Silkworm and Spider Silk Proteins Revealed by Mass Photometry.

  2025cites this paper
• Prey-wrapping spider silk as a model for tough and adaptable protein materials

  2025cites this paper
• Improved alginate bio-ink by recombinant self-assembled cell-sized spider-silk inspired-biopolymer

  2025cites this paper
• Production of recombinant coiled coil silk proteins for materials synthesis.

  2025cites this paper
• Fiber Processing of Recombinant Spider Silk Proteins

  2025cites this paper
• Dimerization and liquid-liquid phase separation of the nonrepetitive domains of pyriform spidroin 1 controls the pyriform silk formation.

  2024cites this paper
• High-strength and ultra-tough supramolecular polyamide spider silk fibers assembled via specific covalent and reversible hydrogen bonds.

  2024cites this paper
• SVX Spider Silk-Inspired Biopolymer and Enhanced Cosmetics Efficacy

  2024cites this paper
• Spider Silk: Rapid, Bottom‐Up Self‐Assembly of MaSp1 into Hierarchically Structured Fibers Through Biomimetic Processing

  2024cites this paper
• Disentangling the Web: An Interdisciplinary Review on the Potential and Feasibility of Spider Silk Bioproduction

  2024cites this paper
• Structural conversion of the spidroin C-terminal domain during assembly of spider silk fibers

  2024cites this paper
• Protein-Based Biological Materials: Molecular Design and Artificial Production

  2023cites this paper
• Disabling spidroin N-terminal homologs' reverse reaction unveils why its intermolecular disulfide bonds have not evolved for 380 million years.

  2023cites this paper
• Engineered spidroin-derived high-performance fibers for diverse applications

  2023cites this paper
• Complexity of Spider Dragline Silk

  2022cites this paper
• Customized Flagelliform Spidroins Form Spider Silk-like Fibers at pH 8.0 with Outstanding Tensile Strength

  2021cites this paper
• Optimized Loopable Translation as a Platform for the Synthesis of Repetitive Proteins

  2021cites this paper
• Progress in the application of spider silk protein in medicine

  2021cites this paper
• Large Scale production of Synthetic Spider Silk Proteins in Escherichia Coli.

  2021cites this paper
• Native-like Flow Properties of an Artificial Spider Silk Dope

  2021cites this paper
• Properties of two spliceoforms of major ampullate spidroin 1 reveal unique functions of N-linker region.

  2020cites this paper
• Advances in Plant-Derived Scaffold Proteins

  2020cites this paper
• Host Systems for the Production of Recombinant Spider Silk.

  2020cites this paper
• Structural characterization and mechanical properties of chimeric Masp1/Flag minispidroins.

  2019cites this paper
• [Functionalized genetic engineered silk-based biomaterials and their applications].

  2019cites this paper
• Production and Purification of Synthetic Minor Ampullate Silk Proteins

  2018cites this paper
• Conservation of a pH-sensitive structure in the C-terminal region of spider silk extends across the entire silk gene family

  2018cites this paper
• Genomic perspectives of spider silk genes through target capture sequencing: Conservation of stabilization mechanisms and homology-based structural models of spidroin terminal regions.

  2018cites this paper
• Using hydrodynamic focusing to predictably alter the diameter of synthetic silk fibers

  2018cites this paper
• Recursive Directional Ligation Approach for Cloning Recombinant Spider Silks.

  2018cites this paper
• Production and Characterization of Artificial Spider Silk Fibers with the Same Toughness as Natural Dragline Silk Fibers

  2018cites this paper
• Characterization of Regenerated Silk Material for Biomimetic Spinning and Film Casting

  2018cites this paper
• Self-assembly of macromolecules in a long and narrow tube

  2018cites this paper
• Production of Synthetic Spider Silk

  2018cites this paper
• Conformation and dynamics of soluble repetitive domain elucidates the initial β-sheet formation of spider silk

  2018cites this paper
• Structural and Mechanical Roles for the C-Terminal Nonrepetitive Domain Become Apparent in Recombinant Spider Aciniform Silk.

  2017cites this paper
• Polyelectrolyte Fiber Assembly of Plant-Derived Spider Silk-like Proteins.

  2017cites this paper
• Duplication and concerted evolution of MiSp-encoding genes underlie the material properties of minor ampullate silks of cobweb weaving spiders

  2017cites this paper
• Silk Materials Functionalized via Genetic Engineering for Biomedical Applications

  2017cites this paper
• Mechanisms of native and artificial spinning of spider silk

  2016cites this paper
• The Structural Characterization Of Argiope Trifasciata Spider Wrapping Silk By Solution-State NMR

  2016cites this paper
• Spider silk as a blueprint for greener materials: a review

  2016cites this paper
• Electrostatics analysis of the mutational and pH effects of the N-terminal domain self-association of the major ampullate spidroin.

  2016cites this paper
• Functionalized silk assembled from a recombinant spider silk fusion protein (Z-4RepCT) produced in the methylotrophic yeast Pichia pastoris.

  2016cites this paper
• Textile cell-free scaffolds for in situ tissue engineering applications

  2016cites this paper
• Chimeric spider silk proteins mediated by intein result in artificial hybrid silks

  2016cites this paper
• Nanomechanics of silk: the fundamentals of a strong, tough and versatile material

  2016cites this paper
• Spider silk-like proteins derived from transgenic Nicotiana tabacum

  2016cites this paper
• Creating Biomaterials from Plant-derived Recombinant Spider Silk-like Proteins

  2016influential citation
• Recombinant Silk Production in Bacteria

  2016cites this paper
• Advances in understanding the properties of spider silk

  2015cites this paper
• Physical and biological regulation of neuron regenerative growth and network formation on recombinant dragline silks.

  2015cites this paper
• Dual Thermosensitive Hydrogels Assembled from the Conserved C-Terminal Domain of Spider Dragline Silk.

  2015cites this paper
• Spider Silk Biocomposites: From Recombinant Protein to Fibers

  2015cites this paper
• Dragline silk: a fiber assembled with low-molecular-weight cysteine-rich proteins.

  2014cites this paper
• Effect of pH on the structure of the recombinant C-terminal domain of Nephila clavipes dragline silk protein.

  2014cites this paper
• Structure and function of C-terminal domain of aciniform spidroin.

  2014cites this paper
• Biotechnology of Silk

  2014cites this paper
• The Power of Recombinant Spider Silk Proteins

  2014cites this paper
• Silk Fiber — Molecular Formation Mechanism, Structure- Property Relationship and Advanced Applications

  2014cites this paper
• Hydrodynamical properties of recombinant spider silk proteins: Effects of pH, salts and shear, and implications for the spinning process.

  2013cites this paper
• Structure-function-property-design interplay in biopolymers: spider silk.

  2013cites this paper
• Mosaic Evolution of Silk Genes in Aliatypus Trapdoor Spiders (Mygalomorphae, Antrodiaetidae)

  2013cites this paper
• Spider silks from plants – a challenge to create native‐sized spidroins

  2013influential citation
• Recombinant production of spider silk proteins.

  2013cites this paper
• Biodegradowalne jedwabne bionanokompozyty (SBC) do terapii celowanej i diagnostyki nowotworów

  2013influential citation
• Minireview Recombinant DNA production of spider silk proteins

  2013cites this paper
• Nanoscale investigations of synthetic spider silk fibers modified by physical and chemical processes

  2013cites this paper
• Recombinant DNA production of spider silk proteins

  2013cites this paper
• The molecular structures of major ampullate silk proteins of the wasp spider, Argiope bruennichi: a second blueprint for synthesizing de novo silk.

  2013cites this paper
• Recombinant Minimalist Spider Wrapping Silk Proteins Capable of Native-Like Fiber Formation

  2012cites this paper
• Molecular mechanisms of amyloid self-regulation

  2012cites this paper
• Ancient Properties of Spider Silks Revealed by the Complete Gene Sequence of the Prey-Wrapping Silk Protein (AcSp1)

  2012cites this paper
• Molecular Evolution of Silk Genes in Mesothele and Mygalomorph Spiders, With Implications for the Early Evolution and Functional Divergence of Silk

  2012cites this paper
• 9.04 – Silks

  2012cites this paper
• A structural view on spider silk proteins and their role in fiber assembly

  2012cites this paper
• Conserved C-terminal domain of spider tubuliform spidroin 1 contributes to extensibility in synthetic fibers.

  2012cites this paper
• [Engineered spider silk: the intelligent biomaterial of the future. Part I].

  2011cites this paper
• Recombinant Dragline Silk-Like Proteins-Expression and Purification.

  2011cites this paper
• Comparison of fibroin cDNAs from webspinning insects: insight into silk formation and function.

  2011cites this paper
• The role of salt and shear on the storage and assembly of spider silk proteins.

  2010cites this paper
• Spider silk proteins: recent advances in recombinant production, structure–function relationships and biomedical applications

  2010cites this paper
• SPIDROIN N-TERMINAL DOMAIN: A PH SENSOR IN THE SPIDER SILK ASSEMBLY PROCESS

  2010cites this paper
• Quantification of the physiochemical constraints on the export of spider silk proteins by Salmonella type III secretion

  2010cites this paper
• Molecular Evolution of Lepidopteran Silk Proteins: Insights from the Ghost Moth, Hepialus californicus

  2010cites this paper
• Untangling spider silk evolution with spidroin terminal domains

  2010cites this paper
• A proposed model for dragline spider silk self-assembly: insights from the effect of the repetitive domain size on fiber properties.

  2010cites this paper
• Self-assembly of spider silk proteins is controlled by a pH-sensitive relay

  2010cites this paper
• A conserved spider silk domain acts as a molecular switch that controls fibre assembly

  2010cites this paper
• Characterization of silk spun by the embiopteran, Antipaluria urichi.

  2009cites this paper
• A Novel Model System for Design of Biomaterials Based on Recombinant Analogs of Spider Silk Proteins

  2009cites this paper
• Left helix of polyproline II type and genesis of β-structures in spidroins 1 and 2 and their recombinant analogs

  2009cites this paper
• Engineered disulfides improve mechanical properties of recombinant spider silk

  2009cites this paper
• Identification and characterization of multiple Spidroin 1 genes encoding major ampullate silk proteins in Nephila clavipes.

  2008cites this paper
• Structural properties of recombinant nonrepetitive and repetitive parts of major ampullate spidroin 1 from Euprosthenops australis: implications for fiber formation.

  2008cites this paper
• Identification and characterization of multiple Spidroin 1 genes encoding major ampullate silk proteins in Nephila clavipes

  2008cites this paper
• A model for the structure of the C-terminal domain of dragline spider silk and the role of its conserved cysteine.

  2007cites this paper
• Spider dragline silk

  2007cites this paper