Essential Oil Composition and Antibacterial Studies of Vitex negundo Linn. Extracts

Essential oils from fresh leaves, flowers and dried fruits of Vitex negundo were obtained by hydrodistillation. Using Soxhlet extractor five successive extracts from dried and powdered leaves were also taken. The chemical constituents of essential oil of leaves, flowers and dried fruits were analyzed by GC-FID and GC/MS techniques. Main constituents identified in leaves oil were δ-guaiene, carryophyllene epoxide and ethyl-hexadecenoate; in flowers oil - α-selinene, germacren-4-ol, carryophyllene epoxide and (E)-nerolidol while fruit oil showed β-selinene, α-cedrene, germacrene D and hexadecanoic acid as the main constituents. β-Caryophyllene was only the constituent identified as common to all three oils. α-Guaiene and guaia-3,7-diene were identified as common constituents in leaf and dried fruit oil while leaf and flower oils showed p -cymene, valencene, caryophyllene epoxide and (E)-nerolidol as common constituent. All the essential oils and successive extracts were evaluated for antibacterial potential against Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa bacterial strains. Each of the essential oils and extracts were found to give promising results against B. subtilis and E. coli. Ethyl acetate and ethanol extracts showed prominent antibacterial activity against all the tested strains. Fruits and leaves oil were found to be most active against E. coli and S. aureus, respectively. Only flowers oil was found to be active against P. aeruginosa.

• Publication year

  2008

• Venue

  Indian Journal of Pharmaceutical Sciences

• Publication date

  2008-07-01

• Fields of study

  Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.4103/0250-474X.44610PMID 20046787PMCID 2792549
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• 정유(Essential Oil)와 그 응용

  2008cited by this paper
• Prostate cancer prevention by silibinin.

  2004cited by this paper
• Anti-inflammatory and analgesic activities of mature fresh leaves of Vitex negundo.

  2003cited by this paper
• Volatile Constituents of Vitex negundo Leaves

  1999cited by this paper
• Volatile constituents of Vitex negundo leaves.

  1999cited by this paper
• CNS activity of Vitex negundo Linn. in mice.

  1999cited by this paper
• Analysis of the essential oils of the leaves of the medicinal plants Vitex negundo var. negundo and Vitex negundo var. purpurescens from India

  1998cited by this paper
• Composition of the Essential Oil of the Leaves of Vitex negundo

  1994cited by this paper
• Chemical investigation and anti-inflammatory activity of Vitex negundo seeds.

  1992cited by this paper
• Sesquiterpene hydrocarbons: from mass confusion to orderly line-up.

  1989cited by this paper

• Essential oil composition and synergistic bioefficacy of medicinal plant essential oils against Tribolium castaneum (Herbst,1797) (Coleoptera: Tenebrionidae)

  2026cites this paper
• Vitex negundo L.: A Comprehensive Exploration of its Phytochemistry, Pharmacological Attributes and Molecular Docking Insights

  2026cites this paper
• Chemical Characterization and In Vitro Bioactivities of Vitex negundo Leaf Extracts.

  2026cites this paper
• Screening of Plant Extracts from the Brazilian Atlantic Forest Reveals a Fraction Rich in Sesquiterpenes from Eugenia astringens as a Potential Antibacterial

  2025cites this paper
• Use of Botanical Extracts as Antiviral Agents Against Tiger Band Disease of Indian Oak Tasar Silkworm

  2025cites this paper
• Study of Antibiotic Resistance Potential of Spiders Bacteriota and Comparison With the Antibacterial Effect of Essential Oils

  2025cites this paper
• Therapeutic potential of Vitex negundo: Bioactive compounds and their role in disease

  2025cites this paper
• Unveiling the mystique of Kulisam (Amulet) with scientific insight

  2024cites this paper
• Fabric pre-treated with Vitex negundo L essential oil as a preventive tool against mosquito bite

  2023cites this paper
• Evaluation of the anti-inflammatory and antioxidant properties, and the isolation and characterization of a new bioactive compound, 3,4,9-trimethyl-7-propyldecanoic acid, from Vitex negundo.

  2023cites this paper
• Valencene, Nootkatone and Their Liposomal Nanoformulations as Potential Inhibitors of NorA, Tet(K), MsrA, and MepA Efflux Pumps in Staphylococcus aureus Strains

  2023cites this paper
• Formulation of Antimicrobial Mouthwash and Comparison with Commercial Mouthwashes

  2023cites this paper
• Preliminary Study on the Antibacterial Activities and Antibacterial Guided Fractionation of Some Common Medicinal Plants Practices in Itum Bahal, Kathmandu Valley of Nepal

  2023cites this paper
• A comprehensive study to explore tyrosinase inhibitory medicinal plants and respective phytochemicals for hyperpigmentation; molecular approach and future perspectives.

  2022cites this paper
• SOME TERPENOID, FLAVONOID, AND MONOCYCLIC AROMATIC COMPOUNDS FROM LEAVES OF VITEX NEGUNDO

  2022cites this paper
• Phytochemical Profile, In Vitro Bioactivity Evaluation, In Silico Molecular Docking and ADMET Study of Essential Oils of Three Vitex Species Grown in Tarai Region of Uttarakhand

  2022cites this paper
• In Silico Study and Excito-Repellent Activity of Vitex negundo L. Essential Oil against Anopheles gambiae

  2022cites this paper
• Specific analytical methods for the extraction of common phytochemical constituents of Vitex negundo Linn: A mini-review

  2021influential citation
• Formulation and evaluation of polyherbal human health amiable hand sanitizer: A need to fight COVID 19

  2021cites this paper
• Discovery of a "Cocktail" of Potential SARS-COV-2 Main Protease Inhibitors through Virtual Screening of Known Chemical Components of Vitex negundo L. ("Lagundi").

  2021cites this paper
• Phyto-pharmacological and Biological Aspects of Vitex negundo Medicinal Plant - A Review

  2021influential citation
• Flavonoids containing Vitex negundo extract displayed calcium channels blocking property underlying its anti-diarrheal and anti-spasmodic activities

  2021cites this paper
• Traditional uses, phytochemistry, and pharmacology of genus Vitex (Lamiaceae)

  2021cites this paper
• A SCANNING ELECTRON MICROSCOPIC EVALUATION OF THE CANAL CLEANLINESS USING ENDOACTIVATOR AND ULTRAX WITH TWO DIFFERENT IRRIGANT SOLUTIONS

  2021cites this paper
• Vitex negundo L. Lamiaceae

  2021cites this paper
• Herbal Therapy for the Management of Seborrheic Dermatitis: A Narrative Review.

  2021cites this paper
• Toxicity of Suaeda maritima (L) against the Scirpophaga incertulas (W) and Xanthomonas oryzae pv. oryzae (Xoo) disease and its non-target effect on earthworm, Eisenia fetida Savigny

  2021cites this paper
• Post-inflammatory hyperpigmentation vs. progressive macular hypomelanosis and their solutions from natural products

  2020cites this paper
• In vitro antimicrobial activity and preliminary phytochemical screening of some plant extracts

  2020cites this paper
• Medicinal Plants with Potent Anti-inflammatory and Anti-arthritic Properties found in Eastern Parts of the Himalaya: An Ethnomedicinal Review

  2020cites this paper
• ANTI-CARIOGENIC POTENTIALS OF VITEX NEGUNDO LINN

  2020influential citation
• Vitex negundo L. Lamiaceae

  2020cites this paper
• Vitex negundo L. leaf extract inhibits IL-6 and TNF-α secretion and phagocytosis in human leukocytes

  2020cites this paper
• Anticancer Screening of the Phytochemicals Present in the Medicinal Plant Vitex Negundo Against Mutant Anaplastic Lymphoma Kinase (ALK) Protein: An In-Silico Approach

  2019cites this paper
• Effect of NAA and BAP on In-Vitro Micropropagation of Vitex negundo L.-A Significant Medicinal Plant

  2019cites this paper
• Identification of new secondary metabolites in Persea americana Miller variety Drymifolia

  2019cites this paper
• ANTI OXIDANT ACTIVITY BY DPPH ASSAY FROM CARICA PAPAYA AQUEOUS LEAVES EXTRACT

  2018cites this paper
• Vitex negundo and its medicinal value

  2018cites this paper
• Antifeedant and ovipositional deterrent activity of medicinal plants of Western Himalaya on Plutella xylostella

  2018cites this paper
• Antibacterial activities of Diospyros blancoi, Phoenix dactylifera and Morus nigra against dental caries causing pathogens: An in vitro study.

  2017cites this paper
• Antifungal response of oral-associated candidal reference strains (American Type Culture Collection) by supercritical fluid extract of nutmeg seeds for geriatric denture wearers: An in vitro screening study

  2017cites this paper
• Estimation of uronic acids using diverse approaches and monosaccharide composition of alkali soluble polysaccharide from Vitex negundo Linn.

  2017cites this paper
• EFFECTIVENESS OF NEEM OIL (Azadirachta indica) AS AN ANTIBACTERIAL AGENT TOWARDS DENTAL CARIES BACTERIA ( Streptococcus mutans )

  2017cites this paper
• NUTRACEUTICALS: A REVIEW

  2017cites this paper
• Coordination behaviours of new (bidentate N,O-chelating) Schiff bases towards copper(II) and nickel(II) metal ions: synthesis, characterization, antimicrobial, antioxidant, and DFT studies

  2017cites this paper
• PHYTOCHEMICAL ANALYSIS AND POWDER MICROSCOPY OF FLOWERS OF VITEX NEGUNDO L – VERBENACEAE

  2017cites this paper
• Himalayan Aromatic Medicinal Plants: A Review of their Ethnopharmacology, Volatile Phytochemistry, and Biological Activities

  2016cites this paper
• Negundo Chastetree (Vitex negundo) Oils

  2016cites this paper
• STUDIES ON ANTIOXIDANT ACTIVITY OF SOME MEDICINAL PLANTS

  2016cites this paper
• Phytochemical Diversity in Essential Oil of Vitex negundo L.Populations from India

  2016cites this paper
• Protective Effect of a Standardized Fraction from Vitex negundo Linn. AgainstAcetaminophen and Galactosamine Induced Hepatotoxicity in Rodents

  2016cites this paper
• Study on essential oils from the leaves of two Vietnamese plants: Jasminum subtriplinerve C.L. Blume and Vitex quinata (Lour) F.N. Williams

  2016cites this paper
• An Overview of Phytotherapeutic Approaches for the Treatment of Tuberculosis.

  2016cites this paper
• Chemical composition of essential oil from the leaves of Gmelina asiatica L.

  2016cites this paper
• Phytochemical and Pharmacological Profile of Vitex negundo

  2015cites this paper
• Antibacterial activity of Vitex negundo L. against a multidrug resistant pathogenic bacterium.

  2015cites this paper
• An Update on Plant Originated Remedies for the Medication of Hyperlipidemia

  2015cites this paper
• IN SILICO ANTIMICROBIAL ACTIVITY OF ACTIVE PHYTOCOMPOUNDS FROM THE LEAF EXTRACT OF VITEX NEGUNDO LINN. AGAINST GLUCOSAMINE 6 PHASPHATE SYNTHASE

  2015cites this paper
• Comparative Evaluation of Antibacterial Efficacy of Six Indian Plant Extracts against Streptococcus Mutans.

  2015cites this paper
• Identification and quantification of phenolic compounds in Vitex negundo L. var. cannabifolia (Siebold et Zucc.) Hand.-Mazz. using liquid chromatography combined with quadrupole time-of-flight and triple quadrupole mass spectrometers.

  2015cites this paper
• In vitro evaluation of six different mouthwashes against six oral pathogens

  2015cites this paper
• Bioassay Guided Fractionation and Hepatoprotective Activity of Oleanolic Acid Acetate Isolated from Vitex negundo Linn.

  2014cites this paper
• Molecular docking study of active phytocompounds from the Molecular docking study of active phytocompounds from the Molecular docking study of active phytocompounds from the Molecular docking study of active phytocompounds from the methanolic leaf extract of

  2014cites this paper
• Anticandidal potential of Crinum asiaticum leaves extract against selected oral and vaginal Candida pathogens

  2014cites this paper
• ANTIBACTERIAL ACTIVITY OF JUNIPERUS COMMUNIS L. AND VITEX NEGUNDO AGAINST XANTHOMONAS AXONOPODIS PV PUNICAE IN VITRO

  2014cites this paper
• Antifungal Properties and Chemical Analysis of Essential Oil from Vitex negundo Seeds

  2014influential citation
• AntifungalPropertiesand Chemical Analysis of Essential Oilfrom Vitex negundo Seeds

  2014cites this paper
• Novel Thin-Layer Chromatographic—Densitometric Method for the Quantification of Betulinic Acid in Nagod (Vitex negundo L.) and Its Herbal Formulations

  2014cites this paper
• Acute and subchronic dermal toxicity of Vitex negundo essential oil

  2014cites this paper
• Available online at www.jibresearch.com

  2014cites this paper
• Isolation of antitrypanosomal compounds from Myanmar medicinal plants [an abstract of dissertation and a summary of dissertation review]

  2014cites this paper
• Protective Effect of p-Cymene on Lipopolysaccharide-Induced Acute Lung Injury in Mice

  2014cites this paper
• Anti-nephrotoxic activity of some medicinal plants from tribal rich pockets of Odisha

  2014cites this paper
• Review on Medicinal Usefulness of Vitex negundo Linn

  2014cites this paper
• The genus Vitex: A review

  2013cites this paper
• Development of standardized formulation of mono herbal (250 mg) capsule.

  2013cites this paper
• In vitro and in vivo bactericidal activity of Vitex negundo leaf extract against diverse multidrug resistant enteric bacterial pathogens.

  2013cites this paper
• Antitumor and antibacterial activity of a crude methanol leaf extract of Vitex negundo L.

  2013cites this paper
• Total phenolic compounds and scavenging activity in Clitoria ternatea and Vitex negundo Linn.

  2013cites this paper
• BIOLOGICAL ACTIVITIES, CHEMICAL CONSTITUENTS AND MEDICINAL VALUE OF VITEX NEGUNDO LINN.

  2013cites this paper
• Informant consensus factor and antimicrobial activity of ethno medicines used by the tribes of wayanad district kerala

  2013cites this paper
• A New Triterpenoid Saponin and Antimicrobial Activity of Ethanolic Extract from Sapindus mukorossi Gaertn.

  2013cites this paper
• Antibacterial activity and composition of essential oils from Pelargonium graveolens L'Her and Vitex agnus-castus L

  2012cites this paper
• p-Cymene Modulates In Vitro and In Vivo Cytokine Production by Inhibiting MAPK and NF-κB Activation

  2012cites this paper
• Influence of gibberellin A3 application, pH of the medium, photoperiod and temperature on the enhancement of in vitro flowering in Vitex negundo L.

  2012cites this paper
• Chemical investigation of Iphigenia stellata Blatter by GC-MS

  2012cites this paper
• The effect of Tamarix ramosissima and Cinnamomum zeylanicum extracts on the growth of Candida spp. isolated from the saliva of type II diabetes mellitus patients

  2012cites this paper
• EXTRACTION AND PHARMACOLOGICAL EVALUATION OF SOME EXTRACTS OF VITEX NEGUNDO LINN.

  2012cites this paper
• PHYTOCHEMICAL AND BIOLOGICAL EVALUATION OF VITEX NEGUNDO LINN: A REVIEW

  2012cites this paper
• Antimicrobial activity and chemical composition of essential oils from Aloysia polystachya (Griseb.) Moldenke grown in Brazil

  2012cites this paper
• Effect of plant compounds on induced activities of defense-related enzymes and pathogenesis related protein in bacterial blight disease susceptible rice plant

  2012cites this paper
• Vitex negundo: - A Chinese Chaste Tree

  2011cites this paper
• Anticonvulsant activity of essential oils isolated from Vitex negundo Linn

  2011cites this paper
• Antibacterial, phytotoxic, insecticidal and cytotoxic potential of Vitex agnus-castus

  2011cites this paper
• Studies on the effect of Vitex negundo leaves against the bacteria isolated from marine ornamental fish

  2011cites this paper
• Insecticidal activity of compounds from the leaves of Vitex negundo (Verbenaceae) against Tribolium castaneum (Coleoptera: Tenebrionidae)

  2011cites this paper
• Xanthine oxidase inhibition of selected Philippine medicinal plants

  2011influential citation
• Antimicrobial Activity of Syzygium aromaticum and Its Bud Oil Against Dental Cares Causing Microorganisms

  2010cites this paper
• The antimicrobial potential of ten often used mouthwashes against four dental caries pathogens

  2010cites this paper
• Correlation of metabolites in the leaf and berries of selected black pepper varieties.

  2010cites this paper