Molecular cloning, characterization and expression analysis of C/EBP α, β and δ in adipose-related tissues and adipocyte of duck (Anas platyrhynchos).

CCAAT/enhancer binding protein α, β, δ (C/EBP α, β, δ) are essential transcriptional factors in regulating adipose development. However, information about their sequence characteristics and functions during adipocyte development still remains scarce in birds. In present study, we found that duck C/EBP α, β, δ differed in their phosphorylation sites and low complexity regions (LCRs) among their orthologs and paralogs. Phylogenetic analysis showed that C/EBP α, β, δ had different evolutionary patterns, and each of duck C/EBP α, β, δ was strikingly diverged from orthologs of other Aves. Results of quantitative real-time PCR exhibited that C/EBP α, β, δ were all highly expressed in duck adipose tissues. Indeed, investigations of changes in both their mRNA levels and lipid droplet content during duck adipocytes differentiation showed that their expression profiles were closely related to cellular lipid accumulation. Furthermore, hierarchical clustering analysis of the C/EBPs and lipid metabolism-related genes expression profiles showed that C/EBP α was clustered with genes related to lipolysis, lipogenesis and fatty acid desaturation, whereas C/EBP β, δ were clustered with genes related to de novo lipogenesis and fatty acid elongation, which were different from mammals. In summary, C/EBP α, β, δ of duck differ from other species in their structures and have different effects on lipid metabolism during adipocytes differentiation. This research serve as a foundation for further investigations about avian C/EBP α, β, δ in adipocytes differentiation and adipose development.

• Publication year

  2018

• Venue

  Comparative Biochemistry and Physiology Part B Comparative Biochemistry

• Publication date

  2018-04-21

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/j.cbpb.2018.04.004PMID 29684538
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Phosphorylation of the FUS low‐complexity domain disrupts phase separation, aggregation, and toxicity

  2017cited by this paper
• Hepatic De Novo Lipogenesis and Regulation of Metabolism

  2016cited by this paper
• Isolation, culture and differentiation of duck (Anas platyrhynchos) preadipocytes

  2015cited by this paper
• CK2 phosphorylation of C/EBPδ regulates its transcription factor activity.

  2015cited by this paper
• Chickens from lines selected for high and low body weight show differences in fatty acid oxidation efficiency and metabolic flexibility in skeletal muscle and white adipose tissue

  2014cited by this paper
• Transcriptional Regulation of Adipocyte Differentiation: A Central Role for CCAAT/Enhancer-binding Protein (C/EBP) β*

  2014cited by this paper
• Inhibition of α-helix-mediated protein-protein interactions using designed molecules.

  2013cited by this paper
• Comparison and characteristics of the formation of different adipose tissues in ducks during early growth.

  2012cited by this paper
• Adipogenesis: from stem cell to adipocyte.

  2012cited by this paper
• Dissecting the role of low-complexity regions in the evolution of vertebrate proteins

  2012cited by this paper
• The CCAAT/enhancer (C/EBP) family of basic-leucine zipper (bZIP) transcription factors is a multifaceted highly-regulated system for gene regulation.

  2011cited by this paper
• Transcriptional factors that promote formation of white adipose tissue.

  2010cited by this paper
• RSK-Mediated Phosphorylation in the C/EBPβ Leucine Zipper Regulates DNA Binding, Dimerization, and Growth Arrest Activity

  2010cited by this paper
• C/EBP transcription factors regulate SREBP1c gene expression during adipogenesis.

  2009cited by this paper
• Genome-Wide Analysis of Histidine Repeats Reveals Their Role in the Localization of Human Proteins to the Nuclear Speckles Compartment

  2009cited by this paper
• Fatty acids but not dexamethasone are essential inducers for chick adipocyte differentiation in vitro.

  2008cited by this paper
• Effect of phosphorylation and S–S bond-induced dimerization on DNA binding and transcriptional activation by C/EBPβ

  2007cited by this paper
• Adipocyte differentiation from the inside out

  2006cited by this paper
• Changes in mRNA expression of regulatory factors involved in adipocyte differentiation during fatty acid induced adipogenesis in chicken.

  2005cited by this paper
• In vitro analysis of partial loss-of-function ZIC2 mutations in holoprosencephaly: alanine tract expansion modulates DNA binding and transactivation.

  2005cited by this paper
• Regulation of stearoyl-CoA desaturases and role in metabolism.

  2004cited by this paper
• Phosphorylation of C/EBPα Inhibits Granulopoiesis

  2004cited by this paper
• Phosphorylation of C/EBPalpha inhibits granulopoiesis.

  2004cited by this paper
• Dominant-negative C/EBP disrupts mitotic clonal expansion and differentiation of 3T3-L1 preadipocytes

  2003cited by this paper
• Evolution of a transcriptional repression domain in an insect Hox protein

  2002cited by this paper
• CCAAT/enhancer-binding proteins: structure, function and regulation.

  2002cited by this paper
• Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.

  2001cited by this paper
• Cytokine induction of Fas gene expression in insulin-producing cells requires the transcription factors NF-kappaB and C/EBP.

  2001cited by this paper
• ADD-1/SREBP-1 is a major determinant of tissue differential lipogenic capacity in mammalian and avian species.

  2001cited by this paper
• The ovine CCAAT-enhancer binding protein delta gene: cloning, characterization, and species-specific autoregulation.

  2000cited by this paper
• Biological Role of the CCAAT/Enhancer-binding Protein Family of Transcription Factors*

  1998cited by this paper
• The Role of C/EBP Genes in Adipocyte Differentiation*

  1998cited by this paper
• Identification of Glycerol-3-phosphate Acyltransferase as an Adipocyte Determination and Differentiation Factor 1- and Sterol Regulatory Element-binding Protein-responsive Gene*

  1997cited by this paper
• Defective adipocyte differentiation in mice lacking the C/EBPβ and/or C/EBPδ gene

  1997cited by this paper
• Defective adipocyte differentiation in mice lacking the C/EBPbeta and/or C/EBPdelta gene.

  1997cited by this paper
• Increased Hepatic Cell Proliferation and Lung Abnormalities in Mice Deficient in CCAAT/Enhancer Binding Protein α*

  1996cited by this paper
• Impaired energy homeostasis in C/EBP alpha knockout mice

  1995cited by this paper
• Cascade regulation of terminal adipocyte differentiation by three members of the C/EBP family of leucine zipper proteins.

  1995cited by this paper
• CCAAT/enhancer binding protein alpha is sufficient to initiate the 3T3-L1 adipocyte differentiation program.

  1994cited by this paper
• Transcriptional activation modulated by homopolymeric glutamine and proline stretches.

  1994cited by this paper
• Molecular cloning of a human cDNA encoding a novel protein, DAD1, whose defect causes apoptotic cell death in hamster BHK21 cells

  1993cited by this paper
• CCAAT/enhancer-binding protein mRNA is translated into multiple proteins with different transcription activation potentials.

  1993cited by this paper
• Reciprocal Regulation of Adipogenesis by Myc and C/EBPα

  1992cited by this paper
• Regulated expression of three C/EBP isoforms during adipose conversion of 3T3-L1 cells.

  1991cited by this paper
• Induction of fatty acid synthetase synthesis in differentiating 3T3-L1 preadipocytes.

  1980cited by this paper
• Evolution illustrated by waterfowl

  1974cited by this paper
• In vitro lipogenesis in the liver and adipose tissues of the female Aylesbury duck at different ages.

  1972cited by this paper
• Theory A Mechanism for the Evolution of Phosphorylation Sites

  year unknowncited by this paper

• Identification of genes and long non-coding RNAs for intramuscular and subcutaneous fat deposition in ducks by transcriptome analysis

  2025cites this paper
• CircADAMTS16 Inhibits Differentiation and Promotes Proliferation of Bovine Adipocytes by Targeting miR-10167-3p

  2023cites this paper
• Expression of Oocyte Vitellogenesis Receptor Was Regulated by C/EBPα in Developing Follicle of Wanxi White Goose

  2022cites this paper
• The Full-Length Transcriptome Provides New Insights Into the Transcript Complexity of Abdominal Adipose and Subcutaneous Adipose in Pekin Ducks

  2021cites this paper
• Genome-Wide Phylogenetic Analysis, Expression Pattern, and Transcriptional Regulatory Network of the Pig C/EBP Gene Family

  2020cites this paper
• Investigation of the CEBPA gene expression pattern and association analysis of its polymorphisms with meat quality traits in chickens

  2020cites this paper
• α, β-Amyrin, a pentacyclic triterpenoid from Protium heptaphyllum suppresses adipocyte differentiation accompanied by down regulation of PPARγ and C/EBPα in 3T3-L1 cells.

  2019cites this paper