miR-490-3p Modulates Cell Growth and Epithelial to Mesenchymal Transition of Hepatocellular Carcinoma Cells by Targeting Endoplasmic Reticulum-Golgi Intermediate Compartment Protein 3 (ERGIC3)*

Background: The role of miR-490-3p in hepatocellular carcinoma (HCC) has not been well clarified. Results: miR-490-3p is up-regulated in HCC and enhances the cell proliferation, migration, invasion abilities, and stimulates the epithelial to mesenchymal transition (EMT) through targeting ERGIC3. Conclusion: miR-490-3p may play an important role in promoting tumor growth and metastasis in HCC. Significance: miR-490-3p may provide beneficial therapeutic strategy for HCC patients. MicroRNAs (miRNAs) are considered to be regulators of various biological processes in cancers, including the epithelial to mesenchymal transition (EMT), which is a key factor in cancer metastasis. In this study, we aimed to clarify the potential roles of miR-490-3p in hepatocellular carcinoma (HCC) cells. Using real-time quantitative RT-PCR, we discovered that miR-490-3p was up-regulated in HCC tissues and cells compared with the adjacent non-tumor tissues and normal cells. We also found that overexpression of miR-490-3p led to an increase in cell proliferation, migration, and invasion abilities and that it contributed to EMT. The inhibition of miR-490-3p had the opposite effect on the cells. We identified ERGIC3 (endoplasmic reticulum-Golgi intermediate compartment protein 3) as a direct target gene for miR-490-3p. Unlike most miRNA-mRNA interactions, miR-490-3p increased ERGIC3 mRNA and protein levels as well as the intensity of expression of the EGFP reporter gene controlled by the 3′-UTR of ERGIC3 mRNA. The up-regulation by miR-490-3p also required the participation of Ago2. The inhibition of miR-490-3p reduced the expression of ERGIC3. Overexpression of ERGIC3 led to the same effect on HCC cells as miR-490-3p overexpression, including EMT. Importantly, silencing ERGIC3 reversed the cellular responses mediated by miR-490-3p overexpression. In conclusion, our study indicated for the first time that miR-490-3p functioned like an oncogenic miRNA in HCC cells and that the inhibition of miR-490-3p might provide an potential treatment approach for HCC patients.

• Publication year

  2012

• Venue

  Journal of Biological Chemistry

• Publication date

  2012-12-04

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1074/jbc.M112.410506PMID 23212913PMCID PMC3567655
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Histone deacetylases activate hepatocyte growth factor signaling by repressing microRNA-449 in hepatocellular carcinoma cells.

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  2012cited by this paper
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  2012cited by this paper
• MicroRNA-214 downregulation contributes to tumor angiogenesis by inducing secretion of the hepatoma-derived growth factor in human hepatoma.

  2012cited by this paper
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  2011cited by this paper
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  2011cited by this paper
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  2011cited by this paper
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  2011cited by this paper
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  2011cited by this paper
• MicroRNA-423 promotes cell growth and regulates G(1)/S transition by targeting p21Cip1/Waf1 in hepatocellular carcinoma.

  2011cited by this paper
• miR-125b is methylated and functions as a tumor suppressor by regulating the ETS1 proto-oncogene in human invasive breast cancer.

  2011cited by this paper
• MicroRNA Control of Invasion and Metastasis Pathways

  2011cited by this paper
• Upregulation of Cyclin B1 by miRNA and its implications in cancer

  2011cited by this paper
• Vimentin in cancer and its potential as a molecular target for cancer therapy

  2011cited by this paper
• MicroRNA-based classification of hepatocellular carcinoma and oncogenic role of miR-517a.

  2011cited by this paper
• Regulation of placenta growth factor by microRNA-125b in hepatocellular cancer.

  2011cited by this paper
• A Parsimonious Model for Gene Regulation by miRNAs

  2011cited by this paper
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  2011cited by this paper
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  2010cited by this paper
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  2010cited by this paper
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  2010cited by this paper
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  2009cited by this paper
• The RNA-binding Protein KSRP Promotes the Biogenesis of a Subset of miRNAs

  2009cited by this paper
• Faculty Opinions recommendation of The RNA-binding protein KSRP promotes the biogenesis of a subset of microRNAs.

  2009cited by this paper
• Regulating the regulators: posttranslational modifications of RNA silencing factors.

  2009cited by this paper
• Biogenesis of small RNAs in animals

  2009cited by this paper
• The miR-200 family and miR-205 regulate epithelial to mesenchymal transition by targeting ZEB1 and SIP1

  2008cited by this paper
• Sorafenib in advanced hepatocellular carcinoma.

  2008cited by this paper
• MicroRNA-10a binds the 5'UTR of ribosomal protein mRNAs and enhances their translation.

  2008cited by this paper
• Sorafenib in advanced hepatocellular carcinoma.

  2008cited by this paper
• Most mammalian mRNAs are conserved targets of microRNAs.

  2008cited by this paper
• MicroRNAs as regulators of epithelial-mesenchymal transition

  2008cited by this paper
• Identification and characterization of endoplasmic reticulum-associated protein, ERp43.

  2007cited by this paper
• AU-rich-element-mediated upregulation of translation by FXR1 and Argonaute 2.

  2007cited by this paper
• Hepatocellular carcinoma pathogenesis: from genes to environment

  2006cited by this paper
• Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs

  2005cited by this paper
• Primary liver cancer: worldwide incidence and trends.

  2004cited by this paper
• Intensified Cloning Efforts Have Revealed Numer

  2004cited by this paper
• The functions of animal microRNAs

  2004cited by this paper
• The nuclear RNase III Drosha initiates microRNA processing

  2003cited by this paper
• MicroRNA maturation: stepwise processing and subcellular localization

  2002cited by this paper
• Epithelial–mesenchymal transitions in tumour progression

  2002cited by this paper
• Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C. elegans developmental timing.

  2001cited by this paper
• [Primary liver cancer].

  1989cited by this paper
• Hepatocellular carcinoma.

  1986cited by this paper

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  2024cites this paper
• miRNAs in HCC, pathogenesis, and targets.

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  2019cites this paper
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  2018cites this paper
• Potential targets and clinical value of miR-490-5p in hepatocellular carcinoma: a study based on TCGA, qRT-PCR and bioinformatics analyses.

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  2018cites this paper
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  2018cites this paper
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  2017cites this paper
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