Overview of Protein Expression by Mammalian Cells

This unit reviews the stages involved in protein production in mammalian cells using a stable‐expression approach. Choice of cell type is discussed, as is transfection of the host cells, methods for selection and amplification of transformants, and growth of cells at appropriate scale for protein production. Since post‐transcriptional modification and intracellular protein transportation are important features of recombinant‐protein production in mammalian cells, some description of these mechanisms is included.

• Publication year

  1997

• Venue

  Current Protocols in Protein Science

• Publication date

  1997-05-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1002/0471140864.ps0509s10PMID 18429190PMCID 7162368
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Role of environmental conditions on the expression levels, glycoform pattern and levels of sialyltransferase for hFSH produced by recombinant CHO cells

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• The effect of protein synthesis inhibitors on the glycosylation site occupancy of recombinant human prolactin

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• Effect of lipid supplements on the production and glycosylation of recombinant interferon-γ expressed in CHO cells

  2004cited by this paper
• Nutrient optimization for high density biological production applications

  2004cited by this paper
• Super-CHO—A cell line capable of autocrine growth under fully defined protein-free conditions

  2004cited by this paper
• Ammonium ion transport—a cause of cell death

  2004cited by this paper
• CO2 in large-scale and high-density CHO cell perfusion culture

  2004cited by this paper
• Selection of Transfected Mammalian Cells

  2003cited by this paper
• Transient Expression of Proteins Using COS Cells

  2002cited by this paper
• Overview of the Vaccinia Virus Expression System

  2002cited by this paper
• Amplification Using CHO Cell Expression Vectors

  2002cited by this paper
• Genetic Engineering of Animal Cells

  2001cited by this paper
• Overview of the vaccinia virus expression system.

  2001cited by this paper
• - α 2 -Macroglobulin

  1998cited by this paper
• Transient Expression of Proteins Using COS Cells

  1998cited by this paper
• Mammalian expression vector with two multiple cloning sites for expression of two foreign genes.

  1997cited by this paper
• Replacement of transferrin by simple iron compounds for MDCK cells grown and subcultured in serum-free medium

  1996cited by this paper
• Cell cycle analysis of foreign gene (β‐galactosidase) expression in recombinant mouse cells under control of mouse mammary tumor virus promoter

  1996cited by this paper
• Getting the glycosylation right: Implications for the biotechnology industry

  1996cited by this paper
• N-linked glycosylation of tissue plasminogen activator in Namalwa cells.

  1995cited by this paper
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  1995cited by this paper
• Removal of Sialic Acid from a Glycoprotein in CHO Cell Culture Supernatant by Action of an Extracellular CHO Cell Sialidase

  1995cited by this paper
• Effect of different cell culture conditions on the polypeptide integrity and N‐glycosylation of a recombinant model glycoprotein

  1995cited by this paper
• Intracellular Folding of Tissue-type Plasminogen Activator

  1995cited by this paper
• Glycosylation and biological activity of CAMPATH-1H expressed in different cell lines and grown under different culture conditions.

  1995cited by this paper
• N-glycans of recombinant human interferon-gamma change during batch culture of chinese hamster ovary cells.

  1995cited by this paper
• Increased cell surface expression and enhanced folding in the endoplasmic reticulum of a mutant erythropoietin receptor.

  1995cited by this paper
• N‐glycans of recombinant human interferon‐γ change during batch culture of chinese hamster ovary cells

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  1994cited by this paper
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  1994cited by this paper
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  1994cited by this paper
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  1994cited by this paper
• Mechanisms of intracellular protein transport

  1994cited by this paper
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  1994cited by this paper
• Calnexin: a membrane-bound chaperone of the endoplasmic reticulum.

  1994cited by this paper
• Monosaccharide and oligosaccharide analysis of isoelectric focusing-separated and blotted granulocyte colony-stimulating factor glycoforms using high-pH anion-exchange chromatography with pulsed amperometric detection.

  1994cited by this paper
• Regulation of UDP-GlcNAc:Gal beta 1-3GalNAc-R beta 1-6-N-acetylglucosaminyltransferase (GlcNAc to GalNAc) in Chinese hamster ovary cells.

  1993influential reference
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  1993influential reference
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• Glycosidase Activities in Chinese Hamster Ovary Cell Lysate and Cell Culture Supernatant

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• Analysis of sequence requirements for protein tyrosine sulfation

  1993cited by this paper
• Foreign gene expression (β‐galactosidase) during the cell cycle phases in recombinant CHO cells

  1993cited by this paper
• Oxidized redox state of glutathione in the endoplasmic reticulum.

  1992influential reference
• High-Level Expression of a Recombinant Antibody from Myeloma Cells Using a Glutamine Synthetase Gene as an Amplifiable Selectable Marker

  1992cited by this paper
• Post-translational chemical modification(s) of proteins.

  1992cited by this paper
• Protein folding in the cell

  1992cited by this paper
• Golgi retention of a trans-Golgi membrane protein, galactosyltransferase, requires cysteine and histidine residues within the membrane-anchoring domain.

  1992cited by this paper
• Hepatitis B surface antigen assembles in a post-ER, pre-Golgi compartment

  1992influential reference
• Molecular dissection of the secretory pathway

  1992cited by this paper
• Glucose‐limited chemostat culture of chinese hamster ovary cells producing recombinant human interferon‐γ

  1992cited by this paper
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  1991cited by this paper
• Purification and characterization of three forms of differently glycosylated recombinant human granulocyte-macrophage colony-stimulating factor.

  1991cited by this paper
• High Level Expression of the Humanized Monoclonal Antibody CAMPATH-1H in Chinese Hamster Ovary Cells

  1991cited by this paper
• Transient Expression of Proteins Using COS Cells

  1991cited by this paper
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  1991cited by this paper
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  1990cited by this paper
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  1990cited by this paper
• Sparged Animal Cell Bioreactors: Mechanism of Cell Damage and Pluronic F‐68 Protection

  1990influential reference
• Expression of Human Glycoproteins in Recombinant Manmalian cells

  1990cited by this paper
• Identification of a consensus motif for retention of transmembrane proteins in the endoplasmic reticulum.

  1990cited by this paper
• The fats of life: the importance and function of protein acylation.

  1990cited by this paper
• Adaptation to non-ammoniagenic medium and selective substrate feeding lead to enhanced yields in animal cell cultures.

  1990cited by this paper
• Use of recombinant DNA technology for engineering mammalian cells to produce proteins.

  1990influential reference
• Perturbation of cellular calcium blocks exit of secretory proteins from the rough endoplasmic reticulum.

  1990cited by this paper
• Effect of ammonium ion and extracellular pH on hybridoma cell metabolism and antibody production

  1990cited by this paper
• Selection and coamplification of heterologous genes in mammalian cells.

  1990influential reference
• Proteolytic processing and physiological regulation.

  1989cited by this paper
• Chinese hamster ovary cell mutants with multiple glycosylation defects for production of glycoproteins with minimal carbohydrate heterogeneity

  1989cited by this paper
• Peroxisome targeting signal of rat liver acyl-coenzyme A oxidase resides at the carboxy terminus

  1989cited by this paper
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  1989cited by this paper
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  1989cited by this paper
• Protein oligomerization in the endoplasmic reticulum.

  1989cited by this paper
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  1989cited by this paper
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  1989cited by this paper
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  1988cited by this paper
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  1988cited by this paper
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  1987cited by this paper
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  1987influential reference
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  1987cited by this paper
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  1986influential reference
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  1986influential reference
• Transcriptional selectivity of viral genes in mammalian cells.

  1986cited by this paper
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  1986influential reference
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  1986influential reference
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  1986influential reference
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  1985cited by this paper
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  1985influential reference
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  1984cited by this paper
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  1984cited by this paper
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  1984cited by this paper
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  1984cited by this paper
• Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus.

  1983cited by this paper

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