Click chemistry for targeted protein ubiquitylation and ubiquitin chain formation

No abstract is available for this paper.

• Publication year

  2015

• Venue

  Nature Protocols

• Publication date

  2015-09-24

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1038/nprot.2015.106PMID 26401915
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Dissecting ubiquitin signaling with linkage-defined and protease resistant ubiquitin chains.

  2014cited by this paper
• Nonenzymatic polyubiquitination of expressed proteins.

  2014cited by this paper
• Site-Specific Bioconjugation of a Murine Dihydrofolate Reductase Enzyme by Copper(I)-Catalyzed Azide-Alkyne Cycloaddition with Retained Activity

  2014cited by this paper
• Nonenzymatic rubylation and ubiquitination of proteins for structural and functional studies.

  2014cited by this paper
• Chemical protein polyubiquitination reveals the role of a noncanonical polyubiquitin chain in DNA damage tolerance.

  2014cited by this paper
• Minimal tags for rapid dual-color live-cell labeling and super-resolution microscopy.

  2014cited by this paper
• Evolved sequence contexts for highly efficient amber suppression with noncanonical amino acids.

  2014cited by this paper
• DUB-resistant ubiquitin to survey ubiquitination switches in mammalian cells.

  2013cited by this paper
• Naturally and synthetically linked lys48 diubiquitin: a QM/MM study

  2013cited by this paper
• A facile strategy for selective incorporation of phosphoserine into histones.

  2013cited by this paper
• Genomically Recoded Organisms Expand Biological Functions

  2013cited by this paper
• Synthetic polyubiquitinated α-Synuclein reveals important insights into the roles of the ubiquitin chain in regulating its pathophysiology

  2013cited by this paper
• Genetically encoded chemical probes in cells reveal the binding path of urocortin-I to CRF class B GPCR.

  2013cited by this paper
• Improving bioorthogonal protein ubiquitylation by click reaction.

  2013cited by this paper
• Nonenzymatic assembly of branched polyubiquitin chains for structural and biochemical studies.

  2013cited by this paper
• Chemoselective Immobilization of Proteins by Microcontact Printing and Bio‐orthogonal Click Reactions

  2013cited by this paper
• The ubiquitin code.

  2012cited by this paper
• Diels-Alder cycloaddition for fluorophore targeting to specific proteins inside living cells.

  2012cited by this paper
• Chemistry and biology of the ubiquitin signal.

  2012cited by this paper
• Fast, cell-compatible click chemistry with copper-chelating azides for biomolecular labeling.

  2012cited by this paper
• Exploiting Ligand-Protein Conjugates to Monitor Ligand-Receptor Interactions

  2012cited by this paper
• Click synthesis of ubiquitin dimer analogs to interrogate linkage-specific UBA domain binding.

  2012cited by this paper
• Chemical synthesis of ubiquitinated peptides with varying lengths and types of ubiquitin chains to explore the activity of deubiquitinases.

  2012cited by this paper
• Nonenzymatic polymerization of ubiquitin: single-step synthesis and isolation of discrete ubiquitin oligomers.

  2012cited by this paper
• Polymerization behavior of a bifunctional ubiquitin monomer as a function of the nucleophile site and folding conditions.

  2012cited by this paper
• Linear polyubiquitin chains

  2011cited by this paper
• SHARPIN forms a linear ubiquitin ligase complex regulating NF-κB activity and apoptosis

  2011cited by this paper
• Generation of a Mono‐ubiquitinated PCNA Mimic by Click Chemistry

  2011cited by this paper
• Total chemical synthesis of a 304 amino acid K48-linked tetraubiquitin protein.

  2011cited by this paper
• RF1 Knockout Allows Ribosomal Incorporation of Unnatural Amino Acids at Multiple Sites

  2011cited by this paper
• Acetylation of lysine 120 of p53 endows DNA-binding specificity at effective physiological salt concentration

  2011cited by this paper
• Expanded click conjugation of recombinant proteins with ubiquitin-like modifiers reveals altered substrate preference of SUMO2-modified Ubc9.

  2011cited by this paper
• Nonenzymatic assembly of natural polyubiquitin chains of any linkage composition and isotopic labeling scheme.

  2011cited by this paper
• An enhanced system for unnatural amino acid mutagenesis in E. coli.

  2010cited by this paper
• Chemically ubiquitylated PCNA as a probe for eukaryotic translesion DNA synthesis.

  2010cited by this paper
• Site-specific incorporation of p-propargyloxyphenylalanine in a cell-free environment for direct protein-protein click conjugation.

  2010cited by this paper
• Nonhydrolyzable ubiquitin-isopeptide isosteres as deubiquitinating enzyme probes.

  2010cited by this paper
• Synthesis of defined ubiquitin dimers.

  2010cited by this paper
• Assembly of k11-linked ubiquitin chains by the anaphase-promoting complex.

  2010cited by this paper
• Engineered diubiquitin synthesis reveals Lys29-isopeptide specificity of an OTU deubiquitinase.

  2010cited by this paper
• Disulfide-directed histone ubiquitylation reveals plasticity in hDot1L activation.

  2010cited by this paper
• Functional ubiquitin conjugates with lysine-epsilon-amino-specific linkage by thioether ligation of cysteinyl-ubiquitin peptide building blocks.

  2009cited by this paper
• Synthesis and microcontact printing of dual end-functionalized mucin-like glycopolymers for microarray applications.

  2009cited by this paper
• Genetic encoding and labeling of aliphatic azides and alkynes in recombinant proteins via a pyrrolysyl-tRNA Synthetase/tRNA(CUA) pair and click chemistry.

  2009cited by this paper
• RAD6-Mediated transcription-coupled H2B ubiquitylation directly stimulates H3K4 methylation in human cells.

  2009cited by this paper
• An in vivo covalent TMP-tag based on proximity-induced reactivity.

  2009cited by this paper
• Analysis and optimization of copper-catalyzed azide-alkyne cycloaddition for bioconjugation.

  2009cited by this paper
• An engineered protein tag for multiprotein labeling in living cells.

  2008cited by this paper
• Chemically ubiquitylated histone H2B stimulates hDot1L-mediated intranucleosomal methylation

  2008cited by this paper
• Synthesis of modified DNA by PCR with alkyne-bearing purines followed by a click reaction.

  2008cited by this paper
• Click Chemistry, A Powerful Tool for Pharmaceutical Sciences

  2008cited by this paper
• HaloTag: a novel protein labeling technology for cell imaging and protein analysis.

  2008cited by this paper
• Site-specific modification of Candida antarctica lipase B via residue-specific incorporation of a non-canonical amino acid.

  2008cited by this paper
• The growing applications of click chemistry.

  2007cited by this paper
• Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators

  2006cited by this paper
• Ubiquitin and ubiquitin-like proteins in cancer pathogenesis

  2006cited by this paper
• Click Chemistry with Polysaccharides

  2006cited by this paper
• Click chemistry as a route to cyclic tetrapeptide analogues: synthesis of cyclo-[Pro-Val-psi(triazole)-Pro-Tyr].

  2006cited by this paper
• A ubiquitin ligase complex assembles linear polyubiquitin chains

  2006cited by this paper
• Ubiquitylation and cell signaling

  2005cited by this paper
• 1,2,3‐Triazole as a Peptide Surrogate in the Rapid Synthesis of HIV‐1 Protease Inhibitors

  2005cited by this paper
• Chemistry in living systems

  2005cited by this paper
• Click to fit: versatile polyvalent display on a peptidomimetic scaffold.

  2005cited by this paper
• Ubiquitin and ubiquitin-like proteins as multifunctional signals

  2005cited by this paper
• Controlled synthesis of polyubiquitin chains.

  2005cited by this paper
• Profiling enzyme activities in vivo using click chemistry methods.

  2004cited by this paper
• Heterocyclic peptide backbone modifications in an alpha-helical coiled coil.

  2004cited by this paper
• Heterocyclic Peptide Backbone Modifications in an α-Helical Coiled Coil

  2004cited by this paper
• Interaction of human DNA polymerase eta with monoubiquitinated PCNA: a possible mechanism for the polymerase switch in response to DNA damage.

  2004cited by this paper
• A heterocyclic peptide nanotube.

  2003cited by this paper
• Bioconjugation by copper(I)-catalyzed azide-alkyne [3 + 2] cycloaddition.

  2003cited by this paper
• The growing impact of click chemistry on drug discovery.

  2003cited by this paper
• Mono- Versus Polyubiquitination: Differential Control of p53 Fate by Mdm2

  2003cited by this paper
• The ubiquitin proteasome system in neurodegenerative diseases: sometimes the chicken, sometimes the egg.

  2003cited by this paper
• Activity-based protein profiling in vivo using a copper(i)-catalyzed azide-alkyne [3 + 2] cycloaddition.

  2003cited by this paper
• A proteomics approach to understanding protein ubiquitination

  2003cited by this paper
• Peptidotriazoles on solid phase: [1,2,3]-triazoles by regiospecific copper(i)-catalyzed 1,3-dipolar cycloadditions of terminal alkynes to azides.

  2002cited by this paper
• A stepwise huisgen cycloaddition process: copper(I)-catalyzed regioselective "ligation" of azides and terminal alkynes.

  2002cited by this paper
• RAD6-dependent DNA repair is linked to modification of PCNA by ubiquitin and SUMO

  2002cited by this paper
• The ubiquitin-proteasome proteolytic pathway: destruction for the sake of construction.

  2002cited by this paper
• C-Terminal Ubiquitination of p53 Contributes to Nuclear Export

  2001cited by this paper
• Click Chemistry: Diverse Chemical Function from a Few Good Reactions.

  2001cited by this paper
• Two RING finger proteins mediate cooperation between ubiquitin‐conjugating enzymes in DNA repair

  2000cited by this paper
• Ubiquitin-activating/conjugating activity of TAFII250, a mediator of activation of gene expression in Drosophila.

  2000cited by this paper
• Recognition of the polyubiquitin proteolytic signal

  2000cited by this paper
• A function for monoubiquitination in the internalization of a G protein-coupled receptor.

  1998cited by this paper
• The ubiquitin system.

  1998cited by this paper
• Protein ubiquitination involving an E1–E2–E3 enzyme ubiquitin thioester cascade

  1995cited by this paper
• High efficiency transformation of E. coli by high voltage electroporation

  1988cited by this paper
• Kinetics and Mechanism of 1,3‐Dipolar Cycloadditions

  1963cited by this paper

• Construction of homologous branched oligomer megamolecules based on linker-directed protein assembly.

  2024cites this paper
• Cellular and Biophysical Applications of Genetic Code Expansion.

  2024cites this paper
• Click Chemistry Methodology: The Novel Paintbrush of Drug Design

  2024cites this paper
• Single-molecule analysis reveals the mechanism of chromatin ubiquitylation by variant PRC1 complexes

  2024cites this paper
• A Site‐Specific Click Chemistry Approach to Di‐Ubiquitylate H1 Variants Reveals Position‐Dependent Stimulation of the DNA Repair Protein RNF168

  2024cites this paper
• Genetic Code Expansion Approaches to Decipher the Ubiquitin Code

  2024cites this paper
• Getting to the Root of Branched Ubiquitin Chains: A Review of Current Methods and Functions.

  2023cites this paper
• Interactome of intact chromatosome variants with site-specifically ubiquitylated and acetylated linker histone H1.2

  2023cites this paper
• Targeted Preparation and NMR Spectroscopic Characterization of Lys11‐Linked Ubiquitin Trimers

  2023cites this paper
• Specifying conformational heterogeneity of multi-domain proteins at atomic resolution.

  2023cites this paper
• Semisynthetic Approach to the Analysis of Tumor Suppressor PTEN Ubiquitination.

  2023cites this paper
• Functional analysis of protein post‐translational modifications using genetic codon expansion

  2023cites this paper
• Generation and Characterization of Site‐Specifically Mono‐Ubiquitylated p53

  2022cites this paper
• Emerging Roles of Non-proteolytic Ubiquitination in Tumorigenesis

  2022cites this paper
• Efficient Protein-Protein Couplings Mediated by Small Molecules under Mild Conditions.

  2022cites this paper
• Deciphering the Structure and Formation of Amyloids in Neurodegenerative Diseases With Chemical Biology Tools

  2022cites this paper
• Site-specific ubiquitylation acts as a regulator of linker histone H1

  2021cites this paper
• Die Länge einer Ubiquitinkette: ein genereller Faktor für die selektive Erkennung durch Ubiquitin‐bindende Proteine

  2020cites this paper
• Die Länge spezifischer Ubiquitin-ketten beeinflusst ihre Erkennung

  2020cites this paper
• Site‐specific ubiquitination of the E3 ligase HOIP regulates apoptosis and immune signaling

  2020cites this paper
• Deciphering protein post-translational modifications using chemical biology tools

  2020cites this paper
• The Ubiquitin Enigma: Progress in the Detection and Chemical Synthesis of Branched Ubiquitin Chains

  2020cites this paper
• The Length of a Ubiquitin Chain: A General Factor for Selective Recognition by Ubiquitin‐Binding Proteins

  2020cites this paper
• Branched Ubiquitination: Detection Methods, Biological Functions and Chemical Synthesis

  2020cites this paper
• Protein Engineering in the Ubiquitin System: Tools for Discovery and Beyond

  2020cites this paper
• Activity-Based Ubiquitin Probes for Investigation of Deubiquitinases

  2020cites this paper
• Cracking the Ubiquitin Code: The Ubiquitin Toolbox.

  2019cites this paper
• Targeted expression and purification of fluorine labelled cold shock protein B by using an auxotrophic strategy.

  2019cites this paper
• Synthetic ubiquitinated proteins meet the proteasome: Distinct roles of ubiquitin in a chain

  2019cites this paper
• Artificially Linked Ubiquitin Dimers Characterised Structurally and Dynamically by NMR Spectroscopy

  2019cites this paper
• Ubiquitin Designer Proteins as a New Additive Generation towards Controlling Crystallization.

  2019cites this paper
• Assembly of branched ubiquitin oligomers by click chemistry.

  2019cites this paper
• SUMOylation and Ubiquitination

  2019cites this paper
• Conformational and functional characterization of artificially conjugated non-canonical ubiquitin dimers

  2019cites this paper
• Chemical biology approaches for studying posttranslational modifications

  2018cites this paper
• Hydrazide Mimics for Protein Lysine Acylation To Assess Nucleosome Dynamics and Deubiquitinase Action.

  2018cites this paper
• Building Peptide Bonds in Haifa: The Seventh Chemical Protein Synthesis (CPS) Meeting

  2018cites this paper
• Advancing the Frontiers of Chemical Protein Synthesis-The 7th CPS Meeting, Haifa, Israel.

  2018cites this paper
• Proteome-wide identification of ubiquitin interactions using UbIA-MS

  2018cites this paper
• The ABCs of PTMs.

  2018cites this paper
• Bioconjugation and Active Site Design of Enzymes Using Non-natural Amino Acids

  2017cites this paper
• Identification of Proteins Interacting with Ubiquitin Chains.

  2017cites this paper
• Site-specific monoubiquitination downregulates Rab5 by disrupting effector binding and guanine nucleotide conversion

  2017cites this paper
• Site-specific conjugation of fibroblast growth factor 2 (FGF2) based on incorporation of alkyne-reactive unnatural amino acid.

  2017cites this paper
• Nonradioactive quantification of autophagic protein degradation with L-azidohomoalanine labeling

  2017cites this paper
• Inverse electron demand Diels-Alder reactions in chemical biology.

  2017cites this paper
• How Chemical Synthesis of Ubiquitin Conjugates Helps To Understand Ubiquitin Signal Transduction.

  2017cites this paper
• Identifizierung von Interaktoren von Ubiquitinketten

  2017cites this paper
• Cu2O spheres as an efficient source of catalytic Cu(I) species for performing azide-alkyne click reactions

  2017cites this paper
• Anionic surfactants enhance click reaction-mediated protein conjugation with ubiquitin.

  2016cites this paper
• Chemical Methods for Encoding and Decoding of Posttranslational Modifications.

  2016cites this paper
• Chemical ubiquitylation of linker histone H1.2 by combining unnatural amino acids with click chemistry

  2016cites this paper
• Chemical Protein Ubiquitylation with Preservation of the Native Cysteine Residues

  2016cites this paper
• Synthetic and semi-synthetic strategies to study ubiquitin signaling

  2016cites this paper
• A Rapid and Versatile Method to Generate Proteins with Defined Ubiquitin Chains

  2016cites this paper
• Chemical ubiquitination for decrypting a cellular code

  2016cites this paper
• Templated self-assembly of the bacterial flagellar motor torque ring in vitro

  2016cites this paper
• A Rapid and Versatile Method for Generating Proteins with Defined Ubiquitin Chains.

  2016cites this paper
• Structural insights into the catalysis and regulation of E3 ubiquitin ligases

  2016cites this paper