Production of biologically active hirudin in plant seeds using oleosin partitioning

No abstract is available for this paper.

• Publication year

  1995

• Venue

  Plant Molecular Biology

• Publication date

  1995-12-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1007/BF00020460PMID 8616216
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Molecular Cloning: A Laboratory Manual

  2001cited by this paper
• Out with the Old, In with the New: Time for a New Drug Development Agency?

  1995cited by this paper
• The development of hirudin as an antithrombotic drug.

  1994cited by this paper
• Regulation of an Arabidopsis oleosin gene promoter in transgenic Brassica napus

  1994cited by this paper
• The disulfide folding pathway of hirudin elucidated by stop/go folding experiments.

  1993cited by this paper
• Anti-hirudin monoclonal antibodies directed toward discontinuous epitopes of the hirudin amino-terminal and epitopes involving the carboxy-terminal hirudin amino acids.

  1993cited by this paper
• Phytase-containing Transgenic Seeds as a Novel Feed Additive for Improved Phosphorus Utilization

  1993cited by this paper
• Nucleotide sequence of an Arabidopsis thaliana oleosin gene.

  1992cited by this paper
• Interaction of the N-terminal region of hirudin with the active-site cleft of thrombin.

  1992cited by this paper
• Nucleotide sequence of an Arabidopsis thaliana oleosin gene

  1992cited by this paper
• Regulation of accumulation of mRNAs encoding a 20-kDa oil-body protein in microspore-derived embryos of Brassica napus

  1992cited by this paper
• Oil bodies and oleosins in seeds

  1992cited by this paper
• Secretion of biologically active leech hirudin from baculovirus-infected insect cells.

  1991cited by this paper
• Effects of jasmonic Acid on embryo-specific processes in brassica and linum oilseeds.

  1991cited by this paper
• Genomic Nucleotide Sequence of a Brassica napus 20-Kilodalton Oleosin Gene.

  1991cited by this paper
• Isolation and physicochemical characterization of the half-unit membranes of oilseed lipid bodies

  1990cited by this paper
• Production of peptides in plant seeds.

  1990cited by this paper
• Production of Correctly Processed Human Serum Albumin in Transgenic Plants

  1990cited by this paper
• Synthesis and secretion of hirudin by Streptomyces lividans

  1990cited by this paper
• Improved binary vectors for Agrobacterium-mediated plant transformation

  1990cited by this paper
• Synthesis of the major oil-body membrane protein in developing rapeseed (Brassica napus) embryos. Integration with storage-lipid and storage-protein synthesis and implications for the mechanism of oil-body formation.

  1989cited by this paper
• Regulation of gene expression during plant embryogenesis.

  1989cited by this paper
• A small-scale procedure for the rapid isolation of plant RNAs.

  1989cited by this paper
• Production of antibodies in transgenic plants

  1989cited by this paper
• Enkephalins Prouduced in Transgenic Plants Using Modified 2S Seed Storage Proteins

  1989influential reference
• High efficiency transformation ofBrassica napus usingAgrobacterium vectors

  1989cited by this paper
• Purification and biochemical characterization of recombinant hirudin produced by Saccharomyces cerevisiae.

  1989cited by this paper
• Biosynthesis of Seed Storage Products during Embryogenesis in Rapeseed, Brassica napus

  1989cited by this paper
• High efficiency transformation of E. coli by high voltage electroporation

  1988cited by this paper
• A modified storage protein is synthesized, processed, and degraded in the seeds of transgenic plants

  1988cited by this paper
• Expression and Secretion in S. Cerevisiae of Biologically Active Leech Hirudin

  1988cited by this paper
• Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa.

  1987cited by this paper
• Expression, secretion and processing of hirudin in E. coli using the alkaline phosphatase signal sequence

  1986cited by this paper
• Cloning and Expression in Escherichia coli of a Synthetic DNA for Hirudin, the Blood Coagulation Inhibitor in the Leech

  1986cited by this paper
• The hypervirulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of T-DNA

  1986cited by this paper
• Cloning and expression of a cDNA coding for the anticoagulant hirudin from the bloodsucking leech, Hirudo medicinalis.

  1986cited by this paper
• A simple and general method for transferring genes into plants.

  1985cited by this paper
• The functional domain of hirudin, a thrombin‐specific inhibitor

  1983influential reference
• DNA sequencing with chain-terminating inhibitors.

  1977cited by this paper
• [69] Hirudin as an inhibitor of thrombin

  1970cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970cited by this paper

• Plant Lipid Droplets and Derived Lipidic Nano‐Assemblies: Structure, Biogenesis and Pharmaceutical Applications

  2025cites this paper
• Exploiting lipid droplet metabolic pathway to foster lipid production: oleosin in focus

  2024cites this paper
• Caleosin/Peroxygenases: multifunctional proteins in plants.

  2023cites this paper
• Optimising expression and extraction of recombinant proteins in plants

  2022cites this paper
• Wolffia arrhiza as a promising producer of recombinant hirudin

  2021cites this paper
• Structure, assembly and application of novel peanut oil body protein extracts nanoparticles.

  2021cites this paper
• Microparticles and Nanoparticles from Plants—The Benefits of Bioencapsulation

  2021influential citation
• Recovery of enzyme byproducts during recombinant protein recovery from transgenic plants

  2020cites this paper
• Reconfiguring Plant Metabolism for Biodegradable Plastic Production

  2020influential citation
• MOLECULAR FARMING AS AN APPROACH FOR PRODUCTION OF USEFUL METABOLITES

  2020cites this paper
• Expression of a functional recombinant vascular endothelial growth factor 165 (VEGF 165 ) in Arabidopsis thaliana

  2019cites this paper
• Biotechnological Production of the Cell Penetrating Antifungal PAF102 Peptide in Pichia pastoris

  2019cites this paper
• Prospects for the Production of Recombinant Therapeutic Proteins and Peptides in Plants: Special Focus on Angiotensin I-Converting Enzyme Inhibitory (ACEI) Peptides

  2019cites this paper
• An interstitial peptide is readily processed from within seed proteins.

  2019cites this paper
• Rice Seeds as Biofactories of Rationally Designed and Cell-Penetrating Antifungal PAF Peptides

  2019cites this paper
• Enhanced Production of Therapeutic Proteins in Plants: Novel Expression Strategies

  2019cites this paper
• Agrobacterium-Mediated Transformation of Lemna minor L. with Hirudin and β-Glucuronidase Genes

  2019cites this paper
• Plant Transformation Strategies

  2018cites this paper
• Focalization of thrombosis and therapeutic perspectives: a memoir

  2018cites this paper
• Modifying seeds to produce proteins

  2018cites this paper
• Redox sensitive protein droplets from recombinant oleosin.

  2018cites this paper
• Plant Lipid Droplets

  2018cites this paper
• Expression of a functional recombinant vascular endothelial growth factor 165 (VEGF165) in Arabidopsis thaliana

  2018cites this paper
• Oil Body-Bound Oleosin-rhFGF-10: A Novel Drug Delivery System that Improves Skin Penetration to Accelerate Wound Healing and Hair Growth in Mice

  2017cites this paper
• Plant-Based Edible Vaccines: Issues and Advantages

  2017cites this paper
• Extraction and purification methods in downstream processing of plant-based recombinant proteins.

  2016cites this paper
• Plants as Bioreactors- A Review

  2016cites this paper
• Transgenic plants as bioreactors to produce substances for medical and veterinary uses

  2016cites this paper
• Molecular Farming Using Transgenic Approaches

  2016cites this paper
• Production of Biologically Active Cecropin A Peptide in Rice Seed Oil Bodies

  2016influential citation
• Amino-Terminus of Oleosin Protein Defines the Size of Oil Bodies -Topological Model of Oleosin-Oil Body Complex

  2015cites this paper
• Expression of bioactive recombinant human fibroblast growth factor 9 in oil bodies of Arabidopsis thaliana.

  2015cites this paper
• The characteristics and potential applications of structural lipid droplet proteins in plants.

  2015cites this paper
• Rice seeds as biofactories of the production of antimicrobial peptides

  2014cites this paper
• Seed-Based Expression Strategies

  2014cites this paper
• Feasibility Study of Rice Growth in Plant Factories

  2014cites this paper
• Molecular Farming: A biotechnological approach in agriculture for production of useful metabolites

  2014cites this paper
• Plant molecular pharming for the treatment of chronic and infectious diseases.

  2014cites this paper
• Glycoengineering in plants for the development of N-glycan structures compatible with biopharmaceuticals

  2014cites this paper
• Molecular Farming in Plants

  2014cites this paper
• Rice Research: Open Access

  2013cites this paper
• Plant Molecular Pharming, Pharmaceuticals for Human Health plant molecular pharming pharmaceuticals for human health

  2013cites this paper
• Induction of recombinant proteins in wheat seed and vegetative tissues

  2013cites this paper
• Production of Pharmaceutical Proteins in Solanaceae Food Crops

  2013cites this paper
• Heterologous production of peptides in plants: fusion proteins and beyond.

  2013cites this paper
• Comparison of Membrane Targeting Strategies for the Accumulation of the Human Immunodeficiency Virus p24 Protein in Transgenic Tobacco

  2013cites this paper
• Molecular Farming in the Decades of Omics

  2013cites this paper
• Engineering lipases and solvents for trans/-esterification of used vegetable oils

  2013cites this paper
• Production of human erythropoietin in transgenic canola employing the technology of oleosin fusion

  2013cites this paper
• Extraction et protéolyse de napines de tourteau de colza : influence de l'état structural de la protéine sur la cinétique de protéolyse, la composition et les fonctionnalités des hydrolysats

  2013cites this paper
• Cyclic Peptides Arising by Evolutionary Parallelism via Asparaginyl-Endopeptidase–Mediated Biosynthesis[C][W]

  2012influential citation
• Species-specific size expansion and molecular evolution of the oleosins in angiosperms.

  2012cites this paper
• Molecular Farming in Plants: A Current Perspective

  2012cites this paper
• Robust preparative-scale extracellular production of hirudin in Escherichia coli and its purification and characterization

  2012cites this paper
• Plant-Based Vaccine Delivery Strategies

  2012cites this paper
• Production of active single-chain antibodies in seeds using trimeric polyoleosin fusion.

  2012cites this paper
• Molecular Farming in Plants: An Overview

  2012cites this paper
• Innovation in Vaccinology

  2012cites this paper
• Strategies for Controlling Plant Diseases and Mycotoxin Contamination Using Antimicrobial Synthetic Peptides

  2012cites this paper
• Plant Biotechnology for Industrial Production

  2012cites this paper
• Using storage organelles for the accumulation and encapsulation of recombinant proteins

  2012cites this paper
• Extraction, isolation and characterisation of oil bodies from pumpkin seeds for therapeutic use.

  2012cites this paper
• Protein body-inducing fusions for high-level production and purification of recombinant proteins in plants.

  2011cites this paper
• Genetic Engineering Approaches for Trait Development in Brassica Oilseed Species

  2011cites this paper
• PLAT MADE PHARMACEUTICALS (PMP's)A PROTEIFACTOR Y: AOVERVIEW

  2011cites this paper
• Oleosin di-or tri-meric fusions with GFP undergo correct targeting and provide advantages for recombinant protein production.

  2011cites this paper
• Expression of biologically active human insulin‐like growth factor 1 in Arabidopsis thaliana seeds via oleosin fusion technology

  2011cites this paper
• 1.32 – Glycomics

  2011cites this paper
• Green Biofactories: Recombinant Protein Production in Plants.

  2010cites this paper
• BIOFACTORIES: PRODUCCIÓ DE PROTEÏNES RECOMBINANTS EN PLANTES

  2010cites this paper
• Use of oil bodies and oleosins in recombinant protein production and other biotechnological applications.

  2010cites this paper
• Biotech Crops for Ecology and Environment

  2010cites this paper
• Strategies for the plant‐based expression of dengue subunit vaccines

  2010cites this paper
• Seed-based expression systems for plant molecular farming.

  2010cites this paper
• Review article: commercialization of whole-plant systems for biomanufacturing of protein products: evolution and prospects.

  2010cites this paper
• Plants as bioreactors: Recent developments and emerging opportunities

  2009influential citation
• Investigation of highly regulated promoters for the production of recombinant proteins in plants

  2009cites this paper
• Dual Chain Avidins – Useful Tools for Fusion Protein Applications

  2009cites this paper
• The production of biopharmaceuticals in plant systems.

  2009cites this paper
• Transgenic Plants Expressing Vaccine and Therapeutic Proteins

  2009cites this paper
• Production de protéines recombinantes par des plantes carnivores génétiquement transformées : application à Drosera rotundifolia et transfert de la technologie à Nepenthes alata

  2009cites this paper
• Structure and function of seed lipid-body-associated proteins.

  2008cites this paper
• Purification of an acidic recombinant protein from transgenic tobacco

  2008cites this paper
• Production of human osteogenic protein-1 in tobacco seeds

  2008cites this paper
• Transgenic plants for animal health: plant-made vaccine antigens for animal infectious disease control

  2008cites this paper
• Production de l’insuline humanisée A partir des graines de carthame Génétiquement modifié

  2008cites this paper
• Large‐scale Protein Production in Plants: Host Plants, Systems and Expression

  2008cites this paper
• The nucleoprotein of Tomato spotted wilt virus as protein tag for easy purification and enhanced production of recombinant proteins in plants.

  2007cites this paper
• Advances in Safflower Biotechnology

  2007cites this paper
• Pharming and transgenic plants.

  2007cites this paper
• Expression of single-chain antibody-barstar fusion in plants.

  2007cites this paper
• Authentic seed-specific activity of the Perilla oleosin 19 gene promoter in transgenic Arabidopsis

  2007cites this paper
• Oleosin fusion expression systems for the production of recombinant proteins

  2007influential citation
• Improving and assessing viral vectors for recombinant protein production in plants

  2006cites this paper
• Highly efficient production of human interferon-α by transgenic cultured rice cells

  2006cites this paper
• GM Issues ORGANIC FARMING AND GENE TRANSFER FROM GENETICALLY MODIFIED CROPS

  2006cites this paper
• Production of biologically active human interferon-α in transgenic rice

  2006cites this paper
• Transgenic Plants for Food Use

  2006cites this paper
• Extraction aqueuse d'huile de colza assistée par hydrolyse enzymatique : optimisation de la réaction, caractérisation de l'émulsion et étude de procédés de déstabilisation

  2006cites this paper
• Plant‐based Expression of Biopharmaceuticals

  2006cites this paper