Binding of anionic lipids to at least three nonannular sites on the potassium channel KcsA is required for channel opening.

No abstract is available for this paper.

• Publication year

  2008

• Venue

  Biophysical Journal

• Publication date

  2008-03-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1529/BIOPHYSJ.107.117507PMID 18024500PMCID PMC2242745
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  2006influential reference
• Lipid interactions with ion channels

  2006cited by this paper
• Anionic phospholipid interactions with the potassium channel KcsA: simulation studies.

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  2003cited by this paper
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  2001cited by this paper
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  2001influential reference
• Amino acid distributions in integral membrane protein structures.

  2001cited by this paper
• Single Streptomyces lividans K+ Channels

  1999cited by this paper
• Functional Asymmetries and Sidedness of Proton Activation

  1999influential reference
• Modulation of Lipid Metabolism and Spiramycin Biosynthesis in Streptomyces ambofaciens Unstable Mutants

  1999cited by this paper
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  1998cited by this paper
• pH-dependent gating in the Streptomyces lividans K+ channel.

  1998cited by this paper
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  1995cited by this paper
• Non-random distribution of amino acids in the transmembrane segments of human type I single span membrane proteins.

  1993cited by this paper
• Quenching of tryptophan fluorescence by brominated phospholipid.

  1990cited by this paper
• Highly cooperative opening of calcium channels by inositol 1,4,5-trisphosphate.

  1988cited by this paper
• Lipids in plants and microbes

  1984cited by this paper
• Annular and non-annular binding sites on the (Ca2+ + Mg2+)-ATPase.

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