The function of the T4 antigen, a marker for a differentiated T cell subset, is not well understood. Our previous observation that a chemoattractant human lymphokine, lymphocyte chemoattractant factor (LCF), which selectively induces motile responses in unactivated T4+ lymphocytes, led us to investigate whether LCF could also induce T4+ cell activation. Because LCF acts selectively on T4+ cells, we next determined whether the T4 antigen has a function in this LCF-induced cellular activation. A T4+ lymphocyte migratory response is induced by divalent anti-T4 antibody, but not by corresponding Fab fragments of the same antibody. Fab fragments of anti-T4 antibody, but not Fab fragments of anti-T3 antibody, block the migratory effect of both LCF and divalent anti-T4. Furthermore, LCF but not divalent anti-T4, evokes the expression of interleukin 2 (IL 2) receptors and HLA-DR antigen on T4+ lymphocytes in 24 hr. These effects are quantitatively similar to those observed by anti-T3 antibody activation. LCF-induced IL 2 receptor expression is blocked by co-incubation with anti-T4 antibody and anti-T4 Fab fragments, whereas anti-T3 activation is not inhibitable by anti-T4 Fab fragments. Because cultured monocytes express the T4 antigen, we investigated the action of LCF on cultured monocyte migration and HLA-DR expression. Induction of monocyte migration by LCF and anti-T4 antibody increases proportionally as T4 antigen expression increases in vitro. This enhanced migration is inhibitable by anti-T4 Fab fragments. Monocyte activation, as measured by augmented HLA-DR expression 24 hr after incubation with LCF, but not anti-T4 antibody, is quantitatively similar to the effects of interferon-gamma. Augmented HLA-DR expression is blocked by anti-T4 Fab fragments but not by antibody to interferon-gamma. These studies indicate that LCF interacts with T4+ lymphocytes and monocytes to induce migration and cellular activation.
Lymphokine activation of T4+ T lymphocytes and monocytes.
W. Cruikshank,J. Berman,A. Theodore,J. Bernardo,D. Center
Published 1987 in Journal of Immunology
ABSTRACT
PUBLICATION RECORD
- Publication year
1987
- Venue
Journal of Immunology
- Publication date
1987-06-01
- Fields of study
Biology, Medicine
- Identifiers
- External record
- Source metadata
Semantic Scholar, PubMed
CITATION MAP
EXTRACTION MAP
CLAIMS
CONCEPTS
- anti-t4 antibody
An antibody directed against the T4 antigen and tested in both divalent and Fab-fragment forms.
Aliases: anti-T4
- anti-t4 fab fragments
Monovalent antigen-binding fragments derived from anti-T4 antibody and used as blocking reagents.
Aliases: anti-T4 Fab
- hla-dr antigen
A class II major histocompatibility antigen used here as a readout of cellular activation.
Aliases: HLA DR antigen, HLA-DR
- interleukin 2 receptor
A lymphocyte activation marker measured after stimulation in the assay system.
Aliases: IL 2 receptor, IL2 receptor, IL-2 receptor
- lymphocyte chemoattractant factor (lcf)
A human lymphokine used in these assays as a chemoattractant stimulus for lymphocytes and monocytes.
Aliases: LCF, lymphokine chemoattractant factor
- monocytes
Cultured mononuclear phagocytes used here to test whether the same stimulus affects non-lymphoid cells expressing T4 antigen.
- t4 antigen
A cell-surface differentiation antigen associated with a T cell subset and detected here on cultured monocytes as well as lymphocytes.
Aliases: T4
- t4+ lymphocytes
Lymphocytes that express the T4 antigen and were examined for migratory and activation responses.
Aliases: T4-positive lymphocytes
REFERENCES
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