Expressing a human proinsulin cDNA in a mouse ACTH-secreting cell. Intracellular storage, proteolytic processing, and secretion on stimulation.

No abstract is available for this paper.

• Publication year

  1983

• Venue

  Cell

• Publication date

  1983-12-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/0092-8674(83)90187-3PMID 6317196
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Membrane traffic in neurons and peptide-secreting cells.

  1983cited by this paper
• Expression of the human insulin gene and cDNA in a heterologous mammalian system.

  1983influential reference
• Chloroquine diverts ACTH from a regulated to a constitutive secretory pathway in AtT-20 cells

  1983cited by this paper
• Expression and regulation of Escherichia coli lacZ gene fusions in mammalian cells.

  1983cited by this paper
• Identification of a 31,500 molecular weight islet cell protease as cathepsin B.

  1983cited by this paper
• A subclass of proteins and sulfated macromolecules secreted by AtT-20 (mouse pituitary tumor) cells is sorted with adrenocorticotropin into dense secretory granules

  1983cited by this paper
• Information within the mature LamB protein necessary for localization to the outer membrane of E coli K12.

  1983cited by this paper
• Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.

  1982cited by this paper
• Introduction of rat growth hormone gene into mouse fibroblasts via a retroviral DNA vector: expression and regulation.

  1982cited by this paper
• Regulated expression of human growth hormone genes in mouse cells.

  1982cited by this paper
• Characterization of pro-opiocortin-converting activity in purified secretory granules from rat pituitary neurointermediate lobe.

  1982cited by this paper
• Biosynthesis of lysosomal hydrolases: their synthesis in bound polysomes and the role of co- and post-translational processing in determining their subcellular distribution

  1982cited by this paper
• Expression from cloned cDNA of cell-surface secreted forms of the glycoprotein of vesicular stomatitis virus in eucaryotic cells.

  1982cited by this paper
• Use of recombinant DNA technology to program eukaryotic cells to synthesize rat proinsulin: a rapid expression assay for cloned genes.

  1982cited by this paper
• Two distinct intracellular pathways transport secretory and membrane glycoproteins to the surface of pituitary tumor cells.

  1982cited by this paper
• Construction of influenza haemagglutinin genes that code for intracellular and secreted forms of the protein

  1982cited by this paper
• Differential regulation of metallothionein-thymidine kinase fusion genes in transgenic mice and their offspring.

  1982cited by this paper
• Post-translational proteolysis in polypeptide hormone biosynthesis.

  1982cited by this paper
• Cell surface expression of the influenza virus hemagglutinin requires the hydrophobic carboxy-terminal sequences.

  1982cited by this paper
• Efficient infection of monkey cells with DNA of simian virus 40.

  1981cited by this paper
• Insulin synthesis in a clonal cell line of simian virus 40-transformed hamster pancreatic beta cells.

  1981cited by this paper
• Secretory granules of an anterior pituitary cell line, AtT-20, contain only mature forms of corticotropin and beta-lipotropin.

  1981cited by this paper
• Biochemical studies of the human thymocyte cell-surface antigens T6, T9 and T10.

  1981cited by this paper
• Secretion of acetylcholinesterase: relation to acetylcholine receptor metabolism.

  1980cited by this paper
• Intracellular transport of secretory and membrane proteins in hepatoma cells infected by vesicular stomatitis virus.

  1980cited by this paper
• Chloroquine inhibits lysosomal enzyme pinocytosis and enhances lysosomal enzyme secretion by impairing receptor recycling

  1980cited by this paper
• Intracellular protein topogenesis.

  1980cited by this paper
• A recognition marker required for uptake of a lysosomal enzyme by cultured fibroblasts.

  1974cited by this paper
• A new technique for the assay of infectivity of human adenovirus 5 DNA.

  1973cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970cited by this paper
• Porcine Proinsulin: Characterization and Amino Acid Sequence

  1968cited by this paper

• Glucose-activated switch regulating insulin analogue secretion enables long-term precise glucose control in type 1 diabetes mice.

  2023cites this paper
• Maturing secretory granules: Where secretory and endocytic pathways converge.

  2021cites this paper
• Insulin Gene Therapy for Treating Type I Diabetes Mellitus: History, Progress and Future Challenges

  2020cites this paper
• Optimasi Waktu Induksi dalam Mengekspresikan Gen Proinsulin secara Intraseluler Menggunakan Inang Pichia pastoris

  2020cites this paper
• Lentivirus mediated pancreatic beta-cell specific insulin gene therapy for STZ-induced Diabetes.

  2020cites this paper
• POMC: The Physiological Power of Hormone Processing.

  2018cites this paper
• III. ENZYMES INVOLVED IN PROCESSING POMC TO DIFFERENT PEPTIDES

  2018cites this paper
• The Prohormone Proinsulin as a Neuroprotective Factor: Past History and Future Prospects

  2018cites this paper
• V1b vasopressin receptor trafficking and signaling: Role of arrestins, G proteins and Src kinase

  2018cites this paper
• Functional Amyloids and their Possible Influence on Alzheimer Disease

  2017cites this paper
• &bgr; Cell Replacement Therapy: The Next 10 Years

  2017cites this paper
• Role of clathrin in dense core vesicle biogenesis

  2017cites this paper
• Determinants for selective transport of exogenously expressed cargo proteins into regulated and constitutive secretory pathways

  2017cites this paper
• TP . 0000000000001937 1 ß cell replacement therapy : the next 10 years

  2017cites this paper
• 60 YEARS OF POMC: Biosynthesis, trafficking, and secretion of pro-opiomelanocortin-derived peptides.

  2016cites this paper
• Beta Cell Therapies for Type 1 Diabetes

  2016cites this paper
• Gene Therapy for Diabetes

  2016cites this paper
• Biosynthesis, Trafficking and Secretion of Pro-opiomelanocortin-derived

  2016cites this paper
• Genetic Engineering of Surrogate β Cells for Treatment of Type 1 Diabetes Mellitus

  2015cites this paper
• Reversal of diabetes following transplantation of an insulin-secreting human liver cell line: Melligen cells

  2015cites this paper
• Cell-penetrating peptides : Uptake mechanism and the role of receptors

  2015cites this paper
• Development of a pancreatic substitute based on genetically engineered intestinal endocrine cells

  2014cites this paper
• Chapter 42 – Bioartificial Pancreas

  2014cites this paper
• Insulin gene therapy from design to beta cell generation

  2012cites this paper
• The Clathrin Adaptor AP-1 and Type II Phosphatidylinositol 4-Kinase are Required for Glue Granule Biogenesis in Drosophila

  2012cites this paper
• Gene and Cell Therapies for Diabetes Mellitus

  2012cites this paper
• Artificial Organs | Pancreas

  2011cites this paper
• Gene therapy in diabetes.

  2010cites this paper
• Mutant proinsulin proteins associated with neonatal diabetes are retained in the endoplasmic reticulum and not efficiently secreted.

  2010cites this paper
• Use of Extra-Pancreatic Tissues for Cell Replacement Therapy for Diabetes

  2008cites this paper
• Gene transfer into the nervous system

  2008cites this paper
• Sorting mechanism of peptide hormones and biogenesis mechanism of secretory granules by secretogranin III, a cholesterol-binding protein, in endocrine cells.

  2008cites this paper
• Etude de l'adressage des protéines GRAs transmembranaires de Toxoplasma gondii aux granules denses et de leur insertion membranaire post-sécrétoire.

  2007cites this paper
• Sequestration of mutated alpha1-antitrypsin into inclusion bodies is a cell-protective mechanism to maintain endoplasmic reticulum function.

  2007cites this paper
• Chapter Forty-Two – Bioartificial Pancreas

  2007cites this paper
• Multiple role of histamine H1-receptor-PKC-MAPK signalling pathway in histamine-stimulated nerve growth factor synthesis and secretion.

  2006cites this paper
• Perspectives in Diabetes Cellular Engineering and Gene Therapy Strategies for Insulin Replacement in Diabetes

  2006cites this paper
• Exogenous expression of glucagon-like peptide 1 receptor and human insulin in AtT-20 corticotrophs confers cAMP-mediated gene transcription and insulin secretion.

  2005cites this paper
• Insulinsezernierende Zellen zur Therapie des Diabetes mellitus

  2005cites this paper
• Use of the Regulated Secretory Pathway to Ease Protein Product Recovery in Animal Cell Culture

  2005cites this paper
• Approaches for the cure of type 1 diabetes by cellular and gene therapy.

  2005cites this paper
• Partial Redirection of Transgenic Human Growth Hormone Secretion from Rat Salivary Glands

  2005cites this paper
• Determination of the Prorelaxin Nucleotide Sequence and Expression of Prorelaxin Messenger Ribonucleic Acid in the Golden Hamster 1

  2004cites this paper
• Processing of mutated human proinsulin to mature insulin in the non-endocrine cell line, CHO

  2004cites this paper
• Towards the development of a bioartificial pancreas: Immunoisolation and NMR monitoring of mouse insulinomas

  2004cites this paper
• Monoclonal anti-vasopressin (VP) antibodies penetrate into VP neurons, in vivo

  2004cites this paper
• Pituitary cell lines and their endocrine applications.

  2004cites this paper
• Cultivation of recombinant, insulin-secreting AtT-20 cells as free and entrapped spheroids

  2004cites this paper
• The Exocrine Protein Trypsinogen into the Secretory Granules of an Studies by Gene Transfer Is Targeted Endocrine Cell Line :

  2003influential citation
• studies by transfer.

  2003influential citation
• Critical independent regions in the VWF propeptide and mature VWF that enable normal VWF storage.

  2003cites this paper
• The P‐selectin cytoplasmic domain directs the cellular storage of a recombinant chimeric factor IX

  2003cites this paper
• Pharmacological Agents That Directly Modulate Insulin Secretion

  2003cites this paper
• Production of recombinant human relaxin 3 in AtT20 cells.

  2003cites this paper
• Gene therapy technology applied to disorders of glucose metabolism: promise, achievements, and prospects.

  2003cites this paper
• Expression of human factor VIII under control of the platelet-specific alphaIIb promoter in megakaryocytic cell line as well as storage together with VWF.

  2003cites this paper
• The insulin-dependent diabetes mellitus, potential of gene therapy

  2003cites this paper
• Biosynthesis and Secretion of Pituitary Hormones: Dynamics and Regulation

  2002cites this paper
• Alteration of the Cytoplasmic Domain of the Membrane-spanning Glycoprotein p 62 of Semliki Forest Virus Does Not Affect Its Polar Distribution in Established Lines of Madin-Darby Canine Kidney Cells

  2002cites this paper
• Gene and cell therapies for diabetes mellitus: strategies and clinical potential.

  2002cites this paper
• Table L Ability to Stimulate Discharge of Pulse-labeled Protein and Unlabeled Amylase from Pancreatic Tissue In Vitro

  2002cites this paper
• PSelectin, A Granule Membrane Protein of Platelets and Endothelial Cells, Follows the Regulated Secretory Pathway in AtT20 Cells

  2002cites this paper
• The role of the D1 domain of the von Willebrand factor propeptide in multimerization of VWF.

  2002cites this paper
• Biosynthesis and post-translational processing of site-directed endoproteolytic cleavage mutants of Pro-CCK in AtT-20 cells.

  2002cites this paper
• Therapeutic strategies for Type 1 and Type 2 diabetes mellitus.

  2002cites this paper
• Calnuc, an EF-hand Ca(2+)-binding protein, is stored and processed in the Golgi and secreted by the constitutive-like pathway in AtT20 cells.

  2002cites this paper
• Trafficking of the Vasopressin and Oxytocin Prohormone Through the Regulated Secretory Pathway

  2001cites this paper
• Gene therapy for diabetes.

  2001cites this paper
• [Cellular engineering and diabetes mellitus].

  2001cites this paper
• Dopamine β-Monooxygenase Signal/Anchor Sequence Alters Trafficking of Peptidylglycine α-Hydroxylating Monooxygenase*

  2001cites this paper
• From stem cells to beta cells: new strategies in cell therapy of diabetes mellitus

  2001cites this paper
• Gene and cell-replacement therapy in the treatment of type 1 diabetes: how high must the standards be set?

  2001cites this paper
• revisão Terapia Gênica para o Diabetes

  2001cites this paper
• Development of β-cell Lines for Transplantation in Type 1 Diabetes Mellitus

  2001cites this paper
• Terapia gênica para o diabetes

  2001cites this paper
• Gene Therapeutic Approaches for β-Cell Replacement

  2001cites this paper
• Association of prohormone convertase 3 with membrane lipid rafts.

  2001cites this paper
• Ingeniería celular y diabetes mellitus

  2001cites this paper
• Basic mechanisms of secretion: sorting into the regulated secretory pathway.

  2000cites this paper
• Regulation of insulin gene transcription.

  2000cites this paper
• Development and Characterization of Pituitary GH3 Cell Clones Stably Transfected with a Human Proinsulin cDNA

  2000cites this paper
• Engineering pancreatic islets

  2000cites this paper
• Calcium homeostasis and nerve growth factor secretion from vascular and bladder smooth muscle cells

  2000cites this paper
• Neurotrophin-3 Sorts to the Constitutive Secretory Pathway of Hippocampal Neurons and Is Diverted to the Regulated Secretory Pathway by Coexpression with Brain-Derived Neurotrophic Factor

  2000cites this paper
• Current perspectives of biotechnological replacement of insulin secreting cells.

  2000cites this paper
• Processing of Frameshifted Vasopressin Precursors

  2000cites this paper
• Trafficking of the Vasopressin and Oxytocin Prohormone Through the Regulated Secretory Pathway

  2000cites this paper
• Cell Type-specific Storage of Dopamine β-Monooxygenase*

  2000cites this paper
• Targeting of the zymogen-granule protein syncollin in AR42J and AtT-20 cells.

  2000cites this paper
• Proinsulin endoproteolysis confers enhanced targeting of processed insulin to the regulated secretory pathway.

  2000cites this paper
• Proinsulin Processing and Insulin Storage

  1999cites this paper
• Differential Sorting of Nerve Growth Factor and Brain-Derived Neurotrophic Factor in Hippocampal Neurons

  1999cites this paper
• Differentiation-induced insulin secretion from nonendocrine cells with engineered human proinsulin cDNA.

  1999cites this paper
• Gene therapy in diabetes mellitus: promises and piffalls.

  1999cites this paper
• Distinct renin isoforms generated by tissue-specific transcription initiation and alternative splicing.

  1999cites this paper
• Engineering cultured insulin-secreting pancreatic B-cell lines

  1999cites this paper
• [Development of insulinoma cells as therapy in insulin-dependent diabetes mellitus].

  1999cites this paper
• Present and potential future use of gene therapy for the treatment of non-insulin dependent diabetes mellitus (Review).

  1999cites this paper
• Prospects for insulin delivery by ex-vivo somatic cell gene therapy

  1999cites this paper
• Cloning from Insulinoma Cells of Synapsin I Associated with Insulin Secretory Granules*

  1999cites this paper