Efficient nitrogen removal in separate coupled-system of anammox and sulfur autotrophic denitrification with a nitrification side-branch under substrate fluctuation.

In order to achieve efficient nitrogen removal, a separate coupled-system of anaerobic ammonia oxidation (anammox) and sulfur autotrophic denitrification (S0-SADN) was established. In this study, the operational feasibility and stability of the coupled-system under substrate fluctuations were investigated. Results showed that the coupled-system improved the total nitrogen removal efficiency (TNRE) to 99.15 ± 0.68%. The tryptophan-like substances in anammox effluent positively impacted the growth of the S0-SADN biofilm. This positive cooperativity boosted the S0-SADN to achieve rapid 12-day startup and stable operation thereafter. The TNRE was determined at 95.27 ± 1.51% and 93.44 ± 0.96% under excessive nitrite and ammonium, respectively. The coupled-system recovered quickly after 21 days of starvation deterioration. To further treat the excessive ammonium, the nitrification side-branch of the coupled-system improved the TNRE to 99.08 ± 0.68%. Extracellular polymeric substances analysis revealed that the anammox and S0-SADN bacteria secreted protein-like substances to resist substrate fluctuation. Microbial community analysis indicated that the stability of bacterial community supported the stability of the coupled-system. These results collectively suggested that the separate coupled-system exhibited excellent performance and provided a platform for practical wastewater treatment in future.

• Publication year

  2019

• Venue

  Science of the Total Environment

• Publication date

  2019-12-15

• Fields of study

  Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1016/j.scitotenv.2019.133929PMID 31442718
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• The revolution of performance, sludge characteristics and microbial community of anammox biogranules under long-term NiO NPs exposure.

  2019cited by this paper
• Coupling of sulfur(thiosulfate)-driven denitratation and anammox process to treat nitrate and ammonium contained wastewater.

  2019cited by this paper
• Roles of sulfite and internal recirculation on organic compound removal and the microbial community structure of a sulfur cycle-driven biological wastewater treatment process.

  2019cited by this paper
• Electron donors for autotrophic denitrification

  2019cited by this paper
• An efficient way to enhance the total nitrogen removal efficiency of the Anammox process by S0-based short-cut autotrophic denitrification.

  2019cited by this paper
• Extraction and characterization of stratified extracellular polymeric substances in Geobacter biofilms.

  2019cited by this paper
• Roles and correlations of functional bacteria and genes in the start-up of simultaneous anammox and denitrification system for enhanced nitrogen removal.

  2019cited by this paper
• Rapid of cultivation dissimilatory perchlorate reducing granular sludge and characterization of the granulation process.

  2019cited by this paper
• Insights into the interactions between triclosan (TCS) and extracellular polymeric substance (EPS) of activated sludge.

  2019cited by this paper
• Sulfur disproportionation tendencies in a sulfur packed bed reactor for perchlorate bio-autotrophic reduction at different temperatures and spatial distribution of microbial communities.

  2019cited by this paper
• Bioreactor performance and microbial community analysis of autotrophic denitrification under micro-aerobic condition.

  2019cited by this paper
• Characterization of EPS compositions and microbial community in an Anammox SBBR system treating landfill leachate.

  2018cited by this paper
• Simultaneous nitrification, denitrification and phosphorus removal in an aerobic granular sequencing batch reactor with mixed carbon sources: reactor performance, extracellular polymeric substances and microbial successions

  2018cited by this paper
• Elemental sulfur-based autotrophic denitrification and denitritation: microbially catalyzed sulfur hydrolysis and nitrogen conversions.

  2018cited by this paper
• A critical review of one-stage anammox processes for treating industrial wastewater: Optimization strategies based on key functional microorganisms.

  2018cited by this paper
• A feasibility study on biological nitrogen removal (BNR) via integrated thiosulfate-driven denitratation with anammox.

  2018cited by this paper
• Performance, microbial community and fluorescent characteristic of microbial products in a solid-phase denitrification biofilm reactor for WWTP effluent treatment.

  2018cited by this paper
• Substrate inhibition and concentration control in an UASB-Anammox process.

  2017cited by this paper
• Effect of thiosulfate on rapid start-up of sulfur-based reduction of high concentrated perchlorate: A study of kinetics, extracellular polymeric substances (EPS) and bacterial community structure.

  2017cited by this paper
• Fluorescence spectroscopy and parallel factor analysis as a dissolved organic monitoring tool to assess treatment performance in drinking water trains.

  2017cited by this paper
• Quantitative and qualitative characterization of extracellular polymeric substances from Anammox enrichment

  2017cited by this paper
• Continuous removal and recovery of tellurium in an upflow anaerobic granular sludge bed reactor.

  2017cited by this paper
• Rapid start-up of the anammox process: Effects of five different sludge extracellular polymeric substances on the activity of anammox bacteria.

  2016cited by this paper
• Rapid start-up of the anammox process by denitrifying granular sludge and the mechanism of the anammox electron transport chain

  2016cited by this paper
• Sulfur-based autotrophic denitrification of drinking water using a membrane bioreactor

  2015cited by this paper
• The starvation tolerance of anammox bacteria culture at 35°C.

  2015cited by this paper
• Comparison of endogenous metabolism during long-term anaerobic starvation of nitrite/nitrate cultivated denitrifying phosphorus removal sludges.

  2015cited by this paper
• Detection of nitrifiers and evaluation of partial nitrification for wastewater treatment: A review.

  2015cited by this paper
• The effect of substrate concentration fluctuation on the performance of high-rate denitrifying reactor.

  2014cited by this paper
• Rapid evolution of adaptive niche construction in experimental microbial populations

  2014cited by this paper
• The effect of sulfide inhibition on the ANAMMOX process.

  2012cited by this paper
• Performance of a pilot‐scale packed bed reactor for perchlorate reduction using a sulfur oxidizing bacterial consortium

  2012cited by this paper
• Characterization of denitrifying granular sludge with and without the addition of external carbon source.

  2012cited by this paper
• Autotrophic Ammonia Removal Processes: Ecology to Technology

  2012cited by this paper
• Component analysis of extracellular polymeric substances (EPS) during aerobic sludge granulation using FTIR and 3D-EEM technologies.

  2012cited by this paper
• Effect of different extraction methods on bound EPS from MBR sludges. Part I: Influence of extraction methods over three-dimensional EEM fluorescence spectroscopy fingerprint

  2010cited by this paper
• Co-existence of anammox and denitrification for simultaneous nitrogen and carbon removal--Strategies and issues.

  2010cited by this paper
• Identifying fouling events in a membrane-based drinking water treatment process using principal component analysis of fluorescence excitation-emission matrices.

  2010cited by this paper
• Extracellular polymeric substances (EPS) of microbial aggregates in biological wastewater treatment systems: a review.

  2010cited by this paper
• Effect of long term anaerobic and intermittent anaerobic/aerobic starvation on aerobic granules.

  2009cited by this paper
• The membrane bioreactor: A novel tool to grow anammox bacteria as free cells

  2008cited by this paper
• Chemolithotrophic denitrification with elemental sulfur for groundwater treatment.

  2007cited by this paper
• Sulfide oxidation under chemolithoautotrophic denitrifying conditions

  2006cited by this paper
• Biodegradability of biofilm extracellular polymeric substances.

  2003cited by this paper
• Fluorescence excitation-emission matrix regional integration to quantify spectra for dissolved organic matter.

  2003cited by this paper
• QUANTIFYING VARIATION IN THE STRENGTHS OF SPECIES INTERACTIONS

  1999cited by this paper
• Programmed cell death in bacterial populations

  1995cited by this paper
• Standard methods for the examination of water and wastewater : supplement to the sixteenth edition

  1988cited by this paper
• Autotrophic denitrification using elemental sulfur

  1987cited by this paper
• Inhibition of nitrification by ammonia and nitrous acid.

  1976cited by this paper
• A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

  1976cited by this paper
• Bergey's Manual of Determinative Bacteriology.

  1975cited by this paper
• Improvement in Anthrone Method for Determination of Carbohydrates

  1952cited by this paper
• Bergey's Manual of Determinative Bacteriology

  1939cited by this paper
• Physiological animal geography

  1911cited by this paper

• Hydroxylamine-Assisted Reactivation of Salinity-Inhibited Partial Denitrification/Anammox Systems: Performance Recovery, Functional Microbial Shifts, and Mechanistic Insights

  2026cites this paper
• Two-stage partial nitritation/Anammox (PN/A) for mainstream wastewater treatment: Achieving high nitrogen removal at elevated loading rates under low-temperature conditions.

  2025cites this paper
• Sulfur-driven partial denitrification coupled with anammox: Advances, optimization, and engineering prospects for sustainable nitrogen removal

  2025cites this paper
• Enhancing Anammox-sulfur autotrophic denitrification coupling with sludge/coal gangue biochar: Nitrogen removal performance and microbial community dynamics

  2025cites this paper
• Deciphering Nitrogen and Sulfur Metabolic Pathways in Thiosulfate-Driven Denitrification Coupled with Anammox for Enhanced Nitrogen Removal

  2025cites this paper
• A critical review of sulfur autotrophic denitrification coupled with anammox.

  2025cites this paper
• Simultaneously ammonium and perchlorate remove via the partial nitrification-anammox coupled sulfur autotrophic system.

  2025cites this paper
• Sulfur autotrophic denitrification as a sustainable nitrogen removal technology to achieve carbon neutrality: Recent advances and optimization strategies

  2025cites this paper
• Deciphering the interactions and metabolic pathways in a two-stage Anammox-SAD coupled system for efficient profound nitrogen removal

  2025cites this paper
• Biochar’s role to achieve multi-pathway nitrogen removal in anammox systems: Insights from electron donation and selective microbial enrichment

  2024cites this paper
• Research on intensive nitrogen removal of municipal sewage by mainstream anaerobic ammonia oxidation process.

  2024cites this paper
• Uncovering pathway and mechanism of simultaneous thiocyanate detoxicity and nitrate removal through anammox and denitrification

  2024cites this paper
• Investigating Potential Microbial Contributors to Enhanced Metabolite Production under Oxygen Perturbations: An Integrated Metagenomic and Metabolomic Approach

  2024cites this paper
• Effect of elemental sulfur on anaerobic ammonia oxidation: Performance and Mechanism.

  2024cites this paper
• Comparative effects of ammonium nitrogen on perchlorate degradation performance under heterotrophic condition with different carbon sources.

  2024cites this paper
• Regulation of nitrogen removal pathways in sulfuric autotrophic denitrification combined with ANAMMOX

  2024cites this paper
• Microbial community structure characteristics and gene distribution of sulfur-siderite/limestone autotrophic denitrification

  2024cites this paper
• A critical review of improving mainstream anammox systems: Based on macroscopic process regulation and microscopic enhancement mechanisms.

  2023cites this paper
• Responses of anammox and sulfur/pyrite autotrophic denitrification in one-stage system to high nitrogen load: Performance, metabolic and bacterial community.

  2023cites this paper
• Coupling sulfur-based denitrification with anammox for effective and stable nitrogen removal: A review.

  2022cites this paper
• A novel completely autotrophic biological treatment process coupled of anammox and sulfur autotrophic (CAS) reactors to remove nitrogen and perchlorate simultaneously

  2022cites this paper
• Efficient nitrogen removal from leachate by coupling Anammox and sulfur-siderite-driven denitrification.

  2022cites this paper
• Biochar enhances partial denitrification/anammox by sustaining high rates of nitrate to nitrite reduction.

  2022cites this paper
• Progresses and challenges in sulfur autotrophic denitrification-enhanced Anammox for low carbon and efficient nitrogen removal

  2022cites this paper
• The feasibility and mechanism of redox-active biochar for promoting anammox performance.

  2022cites this paper
• Semi-starvation fluctuation driving rapid partial denitrification granular sludge cultivation in situ by microorganism exudate metabolites feedbacks.

  2021cites this paper
• Hydroxylamine addition enhances fast recovery of anammox activity suffering Cr(VI) inhibition.

  2021cites this paper
• Perturbation of clopyralid on bio-denitrification and nitrite accumulation: Long-term performance and biological mechanism

  2021cites this paper
• Evaluation of nitrogen removal and N2O emission in a novel anammox coupled with sulfite-driven autotrophic denitrification system: Influence of pH

  2021cites this paper
• Novel biotechnologies for nitrogen removal and their coupling with gas emissions abatement in wastewater treatment facilities.

  2021cites this paper
• Enhanced proteins and amino acids production based on ammonia nitrogen assimilation and sludge increment by the integration of bioadsorption with anaerobic-anoxic-oxic (AAO) process.

  2021cites this paper
• Development of a kinetic model to evaluate thiosulfate-driven denitrification and anammox (TDDA) process.

  2021cites this paper
• Advances and challenges of sulfur-driven autotrophic denitrification (SDAD) for nitrogen removal

  2020cites this paper
• Feasibility of partial denitrification and anammox for removing nitrate and ammonia simultaneously in situ through synergetic interactions.

  2020cites this paper
• Faster removal of nitrite than nitrate in sulfur-based autotrophic denitrification coupled with anammox, affected by the anammox effluent

  2020cites this paper