Performance, microbial community and fluorescent characteristic of microbial products in a solid-phase denitrification biofilm reactor for WWTP effluent treatment.

Microbial products, i.e. extracellular polymeric substance (EPS) and soluble microbial product (SMP), have a significant correlation with microbial activity of biologically based systems. In present study, the spectral characteristics of two kinds of microbial products were comprehensively evaluated in a solid-phase denitrification biofilm reactor for WWTP effluent treatment by using poly (butylene succinate) (PBS) as carbon source. After the achievement of PBS-biofilm, nitrate and total nitrogen removal efficiencies were high of 97.39 ± 1.24% and 96.38 ± 1.1%, respectively. The contents of protein and polysaccharide were changed different degrees in both LB-EPS and TB-EPS. Excitation-emission matrix (EEM) implied that protein-like substances played a significant role in the formation of PBS-biofilm. High-throughput sequencing result implied that the proportion of denitrifying bacteria, including Simplicispira, Dechloromonas, Diaphorobacter, Desulfovibrio, increased to 9.2%, 7.4%, 4.8% and 3.6% in PBS-biofilm system, respectively. According to EEM-PARAFAC, two components were identified from SMP samples, including protein-like substances for component 1 and humic-like and fulvic acid-like substances for component 2, respectively. Moreover, the fluorescent scores of two components expressed significant different trends to reaction time. Gas chromatography-mass spectrometer (GC-MS) implied that some new organic matters were produced in the effluent of SP-DBR due to biopolymer degradation and denitrification processes. The results could provide a new insight about the formation and stability of solid-phase denitrification PBS-biofilm via the point of microbial products.

• Publication year

  2018

• Venue

  Journal of Environmental Management

• Publication date

  2018-12-01

• Fields of study

  Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1016/j.jenvman.2018.09.002PMID 30212684
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Formation of soluble microbial products and their contribution as electron donors for denitrification

  2017cited by this paper
• Qualitative and quantitative analysis of extracellular polymeric substances in partial nitrification and full nitrification reactors.

  2017cited by this paper
• Applying fermentation liquid of food waste as carbon source to a pilot-scale anoxic/oxic-membrane bioreactor for enhancing nitrogen removal: Microbial communities and membrane fouling behaviour.

  2017cited by this paper
• Advanced treatment of municipal secondary effluent by catalytic ozonation using Fe3O4-CeO2/MWCNTs as efficient catalyst

  2017cited by this paper
• Effect of carbon source type on intracellular stored polymers during endogenous denitritation (ED) treating landfill leachate.

  2016cited by this paper
• A Fluorescence Approach to Assess the Production of Soluble Microbial Products from Aerobic Granular Sludge Under the Stress of 2,4-Dichlorophenol

  2016cited by this paper
• Spectroscopic characterization of extracellular polymeric substances from a mixed culture dominated by ammonia-oxidizing bacteria.

  2015cited by this paper
• Protein and polysaccharide content of tightly and loosely bound extracellular polymeric substances and the development of a granular activated sludge floc.

  2015cited by this paper
• Biological denitrification using poly(butylene succinate) as carbon source and biofilm carrier for recirculating aquaculture system effluent treatment.

  2015cited by this paper
• Single stage treatment of saline wastewater with marine bacterial-microalgae consortia in a fixed-bed photobioreactor.

  2015cited by this paper
• Microbial Products of Activated Sludge in Biological Wastewater Treatment Systems: A Critical Review

  2012cited by this paper
• Nitrite accumulation under constant temperature in anoxic denitrification process: The effects of carbon sources and COD/NO(3)-N.

  2012cited by this paper
• Denitrification using PBS as carbon source and biofilm support in a packed-bed bioreactor

  2012cited by this paper
• Comparison of Denitrification Between Paracoccus sp. and Diaphorobacter sp.

  2011cited by this paper
• Biological Denitrification of High Nitrate Processing Wastewaters from Explosives Production Plant

  2011cited by this paper
• Characterization of the size-fractionated biomacromolecules: tracking their role and fate in a membrane bioreactor.

  2011cited by this paper
• Evolution of organic matter in a full-scale composting plant for the treatment of sewage sludge and biowaste by respiration techniques and pyrolysis-GC/MS.

  2011cited by this paper
• Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample

  2010cited by this paper
• Fractionating soluble microbial products in the activated sludge process.

  2010cited by this paper
• Role of extracellular polymeric substances in bioflocculation of activated sludge microorganisms under glucose-controlled conditions.

  2010cited by this paper
• Extraction and structural characteristics of extracellular polymeric substances (EPS), pellets in autotrophic nitrifying biofilm and activated sludge.

  2010cited by this paper
• Formation of soluble microbial products by activated sludge under anoxic conditions

  2010cited by this paper
• PARAFAC modeling of fluorescence excitation-emission spectra for rapid assessment of compost maturity.

  2010cited by this paper
• Gel-forming exopolysaccharides explain basic differences between structures of aerobic sludge granules and floccular sludges.

  2009cited by this paper
• Spectroscopic study of Zn2+ and Co2+ binding to extracellular polymeric substances (EPS) from aerobic granules.

  2009cited by this paper
• Assessing the efficiency of urban waste biocomposting by analytical pyrolysis (Py-GC/MS).

  2009cited by this paper
• The use of crab-shell chitin for biological denitrification: batch and column tests.

  2009cited by this paper
• Removal of nitrate from groundwater by heterotrophic denitrification using the solid carbon source

  2009cited by this paper
• A review on anaerobic-aerobic treatment of industrial and municipal wastewater.

  2009cited by this paper
• Impact of wastewater treatment processes on organic carbon, organic nitrogen, and DBP precursors in effuent organic matter.

  2009cited by this paper
• Extracellular polymeric substances (EPS) properties and their effects on membrane fouling in a submerged membrane bioreactor.

  2009cited by this paper
• Characterizing the interactions between trace metals and dissolved organic matter using excitation-emission matrix and parallel factor analysis.

  2008cited by this paper
• Significance of Chloroflexi in performance of submerged membrane bioreactors (MBR) treating municipal wastewater.

  2007cited by this paper
• Influence of loosely bound extracellular polymeric substances (EPS) on the flocculation, sedimentation and dewaterability of activated sludge.

  2007cited by this paper
• The effects of intermittent aeration on the characteristics of bio-cake layers in a membrane bioreactor.

  2007cited by this paper
• Activity and Community Composition of Denitrifying Bacteria in Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)-Using Solid-phase Denitrification Processes

  2007cited by this paper
• Characterization of extracellular polymeric substances of aerobic and anaerobic sludge using three-dimensional excitation and emission matrix fluorescence spectroscopy.

  2006cited by this paper
• Pyrolytic study of compost and waste organic matter

  2005cited by this paper
• High nitrate removal from synthetic wastewater with the mixed bacterial culture.

  2005cited by this paper
• Application of polyhydroxyalkanoates for denitrification in water and wastewater treatment

  2003cited by this paper
• A unified theory for extracellular polymeric substances, soluble microbial products, and active and inert biomass.

  2002cited by this paper
• Drinking water denitrification by a membrane bio-reactor.

  2002cited by this paper
• Anaerobic benzene oxidation coupled to nitrate reduction in pure culture by two strains of Dechloromonas

  2001cited by this paper
• Influence of carbon source on nitrate removal of contaminated groundwater in a denitrifying submerged filter.

  2000cited by this paper
• Biodegradable polymers as solid substrate and biofilm carrier for denitrification in recirculated aquaculture systems

  2000cited by this paper
• Physico-chemistry of initial microbial adhesive interactions--its mechanisms and methods for study.

  1999cited by this paper
• Denitrification kinetics of high nitrate concentration water: pH effect on inhibition and nitrite accumulation

  1998cited by this paper
• PARAFAC. Tutorial and applications

  1997cited by this paper
• APHA, A. P. H. A. : Standard Methods for the Examination of water and Wastewater.

  1995cited by this paper
• Characteristics and energy requirements of an alpha-aminoisobutyric acid transport system in Streptococcus lactis

  1976cited by this paper

• Food waste treatment by black soldier fly larvae (BSFL) and pilot-scale aerobic composting of the BSFL frass using horizontal mechanical reactor

  2026cites this paper
• Impact of batch seeding on the development of biological activated carbon filter for the simultaneous removal of organics, nitrogen, and emerging contaminants from secondary effluents

  2026cites this paper
• Oxygen stress drives overcompensation of carbon sources for enhanced polymer denitrification.

  2026cites this paper
• Synergistic mechanisms of polypyrrole in enhancing phosphate bioreduction and mitigating the toxicity of generated phosphine in wastewater

  2025cites this paper
• In situ formed colloidal manganese dioxide enhances permanganate oxidation for sulfide control in sewers

  2025cites this paper
• Influence of biodegradable microplastic in biological treatment of wastewater: performance evaluation of poly(butylene succinate) microparticle degradation in MBBR

  2025cites this paper
• Unraveling synergistic cascade inhibition of methane production in anaerobic digestion system by polyethylene microplastics and domestic sewage: Physical adsorption, metabolic disruption, and microbial community decoupling.

  2025cites this paper
• Microplastic Diversity as a Potential Driver of Soil Denitrification Shifts.

  2025cites this paper
• Anammox Biofilm Reactor Inoculated With Denitrification-anaerobic Sludge to Achieve Rapid Start-up and Efficient Treatment of Low Concentration Wastewater

  2025cites this paper
• Ultrahigh carbon utilization in symbiotic Biofilm-Sludge denitrification systems using polymers as sole electron donors.

  2024cites this paper
• The effect of carbon source produced by modified corncob fermentation on denitrification

  2024cites this paper
• The Effect of Electricity Generation on the Performance of Microbial Fuel Cells for Anammox

  2024cites this paper
• A novel derivative synchronous fluorescence method for the rapid, non-destructive and intuitive differentiation of denitrifying bacteria.

  2024cites this paper
• Pilot study on the filling rate of parallel AA‐MBBR fillers in plateau areas

  2024cites this paper
• Rhamnolipid-assisted solid-phase denitrification process for enhanced nitrate removal of recirculating mariculture system water

  2024cites this paper
• Research Progress on Solid-phase Electron Donors for the Denitrification of Wastewater: A Review

  2024cites this paper
• Unveiling the Coupling Mechanism between Central Carbon and Nitrogen Metabolism of Pseudomonas stutzeri: Polyhydroxyalkanoate-Enhanced Electron Supply for Denitrification

  2024cites this paper
• Revealing the Viable Microbial Community of Biofilm in a Sewage Treatment System Using Propidium Monoazide Combined with Real-Time PCR and Metagenomics

  2024cites this paper
• Achieving high nitrogen and antibiotics removal efficiency by nZVI-C in partial nitritation/anammox system with a single-stage membrane-aerated biofilm reactor.

  2024cites this paper
• Multi-omics analysis of nitrifying sludge under carbon disulfide stress: Nitrification performance and molecular mechanisms.

  2024cites this paper
• Groundwater denitrification enhanced by a hydrogel immobilized iron/solid carbon source: impact on denitrification and substrate release performance.

  2024cites this paper
• High nitrogen removal and electricity generation of anaerobic fluidized bed coupled microbial fuel cell for Anammox

  2024cites this paper
• The clean nitrogen removal process based on solid carbon sources: Research progress and outlook

  2023cites this paper
• Insights into solid phase denitrification in wastewater tertiary treatment:the role of solid carbon source in carbon biodegradation and heterotrophic denitrification.

  2023cites this paper
• Effects of adding different ratios of residual sludge and food waste co-anaerobic fermentation liquid to AAO wastewater treatment process

  2023cites this paper
• Simultaneous Removal of Nitrate and Phosphate in a Pyrrhotite and Sulfur-Circulating Packed Bed Reactor

  2023cites this paper
• Sustainable technology: potential of biomass (Bambusa tuldoides) for biological denitrification of wastewater generated in shrimp farming

  2023cites this paper
• Effect of antibiotics on the performance of moving bed biofilm reactor for simultaneous removal of nitrogen, phosphorus and copper(II) from aquaculture wastewater.

  2023cites this paper
• A novel sulfur autotrophic denitrification in-situ coupled sequencing batch reactor system to treat low carbon to nitrogen ratio municipal wastewater: Performance, niche equilibrium and pollutant removal mechanisms.

  2023cites this paper
• Insights into the relationship between denitrification and organic carbon release of solid-phase denitrification systems: mechanism and microbial characteristics.

  2022cites this paper
• Removal performance and dissolved organic matter biodegradation characteristics in advection ecological permeable dam reactor

  2022cites this paper
• Te(IV) bioreduction in the sulfur autotrophic reactor: Performance, kinetics and synergistic mechanism.

  2022cites this paper
• Formation of anaerobic granular sludge (AnGS) to treat high-strength perchlorate wastewater via anaerobic baffled reactor (ABR) system: Electron transfer characteristic, bacterial community and positive feedback mechanism.

  2022cites this paper
• Impacts of bio-carriers on the characteristics of cake layer and membrane fouling in a novel hybrid membrane bioreactor for treating mariculture wastewater.

  2022cites this paper
• Effect of biofilm colonization on Pb(II) adsorption onto poly(butylene succinate) microplastic during its biodegradation.

  2022cites this paper
• Sustainable Plant Growth Promotion and Chemical Composition of Pyroligneous Acid When Applied with Biochar as a Soil Amendment

  2022cites this paper
• A new LDH based sustained-release carbon source filter media to achieve advanced denitrogenation of low C/N wastewater at low temperature.

  2022cites this paper
• Influence mechanisms of macro‐infrastructure on micro‐environments in the recirculating aquaculture system and biofloc technology system

  2022cites this paper
• Solid slow-release carbon source assembled microbial fuel cell for promoting superior nitrogen removal in an aerobic granular sludge bioreactor.

  2022cites this paper
• The potent effects of polyoxometalates (POMs) on controlling sulfide and methane production from sewers

  2022cites this paper
• The research progress, hotspots, challenges and outlooks of solid-phase denitrification process.

  2022cites this paper
• The effect and mechanism of molybdenum electron shuttle on perchlorate bioreduction

  2022cites this paper
• A designed moderately thermophilic consortia with a better performance for leaching high grade fine lead-zinc sulfide ore.

  2021cites this paper
• Impact of light on anoxic/oxic reactors: performance, quorum sensing, and metagenomic characteristics.

  2021cites this paper
• Solid-phase denitrification in high salinity and low-temperature wastewater treatment.

  2021cites this paper
• Effect of hydraulic retention time on solid-phase denitrification reactor in recirculating aquaculture system

  2021cites this paper
• Enhanced simultaneous removal of nitrate and phosphate using novel solid carbon source/zero-valent iron composite

  2021cites this paper
• Study on the electron transfer capability of porphyrin ring and the mechanisms in the catalytic denitrification

  2021cites this paper
• Research progress in solid carbon source-based denitrification technologies for different target water bodies.

  2021cites this paper
• Nitrogen removal performance and microbial diversity of bioreactor packed with cellulosic carriers in recirculating aquaculture system

  2021cites this paper
• Multifaceted synergistic electron transfer mechanism for enhancing denitrification by clay minerals.

  2021cites this paper
• Biodegradation of carbon tetrachloride from groundwater in an upflow solid-phase biofilm system

  2020cites this paper
• Heterotrophic denitrification strategy for marine recirculating aquaculture wastewater treatment using mariculture solid wastes fermentation liquid as carbon source: Optimization of COD/NO3--N ratio and hydraulic retention time.

  2020cites this paper
• Fate and role of fluorescence moieties in extracellular polymeric substances during biological wastewater treatment: A review.

  2020cites this paper
• Effective control of the carbon release of starch/polyvinyl alcohol based on a polyamide coating in solid-phase denitrification

  2020cites this paper
• Effect of solid-liquid separation on food waste fermentation products as external carbon source for denitrification

  2020cites this paper
• Process safety and environmental protection: An optimized solid phase denitrification filter by using activated carbon fibers for secondary effluent treatment

  2020cites this paper
• Distinct rhizobacterial functional assemblies assist two Sedum alfredii ecotypes to adopt different survival strategies under lead stress.

  2020cites this paper
• Simultaneous nitrate and dissolved organic matter removal from wastewater treatment plant effluent in a solid-phase denitrification biofilm reactor.

  2020cites this paper
• Influence of salinity stress on bacterial community composition and β-glucosidase activity in a tropical estuary: Elucidation through microcosm experiments.

  2020cites this paper
• The influence mechanism of temperature on solid phase denitrification based on denitrification performance, carbon balance, and microbial analysis.

  2020cites this paper
• Aerobic biodegradation of p-nitrophenol in a nitrifying sludge bioreactor: System performance, sludge property and microbial community shift.

  2020cites this paper
• Biological treatment of high strength ammonia wastewater containing 2,4-dichlorophenol in a membrane bioreactor: System performance and microbial community

  2019cites this paper
• Efficient nitrogen removal in separate coupled-system of anammox and sulfur autotrophic denitrification with a nitrification side-branch under substrate fluctuation.

  2019cites this paper
• Insights into the effects of carbon source on sequencing batch reactors: Performance, quorum sensing and microbial community.

  2019cites this paper
• Effect of plant-based carbon source supplements on denitrification of synthetic wastewater: focus on the microbiology

  2019cites this paper
• Effects of carbon availability in a woody carbon source on its nitrate removal behavior in solid-phase denitrification.

  2019cites this paper
• Nitrogen removal in a combined aerobic granular sludge and solid-phase biological denitrification system: System evaluation and community structure.

  2019cites this paper