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Abscisic acid positively regulates L-Arabinose metabolism to inhibit seed germination through ABI4-mediated transcriptional promotions of MUR4 in Arabidopsis thaliana.

Jingwei Yan,Lin Fang,Lan Yang,Huan He,Yun Huang,Ya Liu,Aying Zhang

Published 2019 in New Phytologist

ABSTRACT

L-Ara is a major monosaccharide in plant polysaccharides and glycoproteins, and functions in plant growth and development. However, the potential role of L-Ara during abscisic acid (ABA)-mediated seed germination has been largely ignored. Here, our results showed a function of L-Ara during ABA-mediated seed germination. ABA slowed down the reduction of L-Ara in seed cell wall, and exogenous L-Ara aggravated the inhibition of ABA on germination. We further found that MUR4, encoding UDP-D-XYLOSE 4-EPIMERASE 1, played a vital role in ABA-mediated germination. MUR4 was highly expressed in embryo and induced by ABA in both seeds and seedlings. Overexpression of MUR4 conferred hypersensitive seed germination and early post-germination growth to ABA. Further analysis revealed that ABSCISIC ACID INSENSITIVE (ABI4) positively modulated the MUR4 expression by directly binding the CE1 motif of MUR4 promoter. Consistently, abi4-1 mutant had a lower L-Ara content in seed cell wall, while a higher L-Ara content in seed cell wall was observed in ABI4 overexpressors. Genetic analysis suggested that overexpression of MUR4 in abi4-1 partly restored the ABA sensitivity of abi4-1. We established the link between ABA and L-Ara during ABA-mediated seed germination and cotyledon greening in Arabidopsis, and revealed the potential molecular mechanism. This article is protected by copyright. All rights reserved.

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