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Timing of Heparin Addition to the Biomolecular Corona Influences the Cellular Uptake of Nanocarriers.

C. Champanhac,J. Simon,K. Landfester,V. Mailänder

Published 2019 in Biomacromolecules

ABSTRACT

Few studies have considered the interaction of nanocarriers with drugs and the implications for their individual efficiency. Here, we demonstrate that heparin, a common anticoagulant, interacts with nanocarriers. Hence, nanocarriers, pre-coated with heparin and plasma in different conditions, were incubated with cancer cells, as well as primary cells from human blood. The relation between the timing of the heparin's addition to the nanocarrier and the cellular uptake extent was assessed by flow cytometry. Through proteomics the effect of heparin on the biomolecular corona composition was determined. We found that HeLa cells, monocytes and macrophages reacted differently to the presence of heparin: the uptake of the pre-coated nanoparticles decreased for HeLa and primary monocytes, while it increased for macrophages. Heparin induced no obvious change in the protein corona composition; thus, we suggest that heparin itself, through its adsorption on the nanoparticle, was responsible for the change of uptake.

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