Targeted gene disruption in Candida albicans wild‐type strains: the role of the MDR1 gene in fluconazole resistance of clinical Candida albicans isolates

No abstract is available for this paper.

• Publication year

  2000

• Venue

  Molecular Microbiology

• Publication date

  2000-05-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1046/j.1365-2958.2000.01899.xPMID 10844673
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Activation of the Multiple Drug Resistance GeneMDR1 in Fluconazole-Resistant, Clinical Candida albicans Strains Is Caused by Mutations in atrans-Regulatory Factor

  2000cited by this paper
• Sequential gene disruption in Candida albicans by FLP‐mediated site‐specific recombination

  1999cited by this paper
• Host‐induced, stage‐specific virulence gene activation in Candida albicans during infection

  1999cited by this paper
• Isolation of a Putative Candida albicansTranscriptional Regulator Involved in Pleiotropic Drug Resistance by Functional Complementation of a pdr1 pdr3 Mutation inSaccharomyces cerevisiae

  1999cited by this paper
• Molecular aspects of fluconazole resistance development in Candida albicans

  1999cited by this paper
• Multiple Molecular Mechanisms Contribute to a Stepwise Development of Fluconazole Resistance in Clinical Candida albicans Strains

  1998cited by this paper
• Amino Acid Substitutions in the Cytochrome P-450 Lanosterol 14α-Demethylase (CYP51A1) from Azole-Resistant Candida albicans Clinical Isolates Contribute to Resistance to Azole Antifungal Agents

  1998cited by this paper
• Altered Expression of Selectable Marker URA3 in Gene-Disrupted Candida albicans Strains Complicates Interpretation of Virulence Studies

  1998cited by this paper
• Diethylcarbamazine-related antimicrobial activity in Mycobacterium tuberculosis-infected blood.

  1998cited by this paper
• Distinct Patterns of Gene Expression Associated with Development of Fluconazole Resistance in Serial Candida albicansIsolates from Human Immunodeficiency Virus-Infected Patients with Oropharyngeal Candidiasis

  1998cited by this paper
• Multidrug‐Resistant Transport Proteins in Yeast: Complete Inventory and Phylogenetic Characterization of Yeast Open Reading Frames within the Major Facilitator Superfamily

  1997cited by this paper
• Clustered amino acid substitutions in the yeast transcription regulator Pdr3p increase pleiotropic drug resistance and identify a new central regulatory domain

  1997cited by this paper
• Increased mRNA levels of ERG16, CDR, and MDR1 correlate with increases in azole resistance in Candida albicans isolates from a patient infected with human immunodeficiency virus

  1997cited by this paper
• Saccharomyces cerevisiae Basic Region-Leucine Zipper Protein Regulatory Networks Converge at the ATR1 Structural Gene*

  1997cited by this paper
• The presence of an R467K amino acid substitution and loss of allelic variation correlate with an azole-resistant lanosterol 14alpha demethylase in Candida albicans

  1997cited by this paper
• Molecular and phenotypic characterization of yeast PDR1 mutants that show hyperactive transcription of various ABC multidrug transporter genes

  1997cited by this paper
• Cloning of Candida albicans genes conferring resistance to azole antifungal agents: characterization of CDR2, a new multidrug ABC transporter gene.

  1997cited by this paper
• Multiple-drug-resistance phenomenon in the yeast Saccharomyces cerevisiae: involvement of two hexose transporters

  1997cited by this paper
• The yeast ATP binding cassette (ABC) protein genes PDR10 and PDR15 are novel targets for the Pdr1 and Pdr3 transcriptional regulators

  1997cited by this paper
• Overexpression of a cloned IMP dehydrogenase gene of Candida albicans confers resistance to the specific inhibitor mycophenolic acid

  1997cited by this paper
• AP1-mediated Multidrug Resistance in Saccharomyces cerevisiae Requires FLR1 Encoding a Transporter of the Major Facilitator Superfamily*

  1997cited by this paper
• Susceptibilities of Candida albicans multidrug transporter mutants to various antifungal agents and other metabolic inhibitors

  1996cited by this paper
• The ATP‐binding cassette multidrug transporter Snq2 of Saccharomyces cerevisiae: a novel target for the transcription factors Pdr1 and Pdr3

  1996cited by this paper
• Susceptibility testing of fungi: current status of correlation of in vitro data with clinical outcome

  1996cited by this paper
• Resistance of Candida species to fluconazole

  1995cited by this paper
• Evidence implicating phospholipase as a virulence factor of Candida albicans

  1995cited by this paper
• Expression of an ATP-binding cassette transporter-encoding gene (YOR1) is required for oligomycin resistance in Saccharomyces cerevisiae

  1995cited by this paper
• Mechanisms of resistance to azole antifungal agents in Candida albicans isolates from AIDS patients involve specific multidrug transporters

  1995influential reference
• Cadmium tolerance mediated by the yeast AP-1 protein requires the presence of an ATP-binding cassette transporter-encoding gene, YCF1.

  1994cited by this paper
• Fluconazole-resistant recurrent oral candidiasis in human immunodeficiency virus-positive patients: persistence of Candida albicans strains with the same genotype

  1994cited by this paper
• Emergence of fluconazole-resistant strains of Candida albicans in patients with recurrent oropharyngeal candidosis and human immunodeficiency virus infection

  1994influential reference
• PDR5, a novel yeast multidrug resistance conferring transporter controlled by the transcription regulator PDR1.

  1994cited by this paper
• Transcriptional control of the yeast PDR5 gene by the PDR3 gene product

  1994cited by this paper
• Candida albicans gene encoding resistance to benomyl and methotrexate is a multidrug resistance gene

  1994cited by this paper
• Expression of seven members of the gene family encoding secretory aspartyl proteinases in Candida albicans

  1994cited by this paper
• Molecular mechanisms of drug resistance in fungi.

  1994cited by this paper
• Isogenic strain construction and gene mapping in Candida albicans.

  1993cited by this paper
• High-frequency phenotypic switching in Candida albicans.

  1993cited by this paper
• Interaction of the yeast pleiotropic drug resistance genes PDR1 and PDR5

  1992cited by this paper
• Adherence and receptor relationships of Candida albicans.

  1991cited by this paper
• Analysis of a Candida albicans gene that encodes a novel mechanism for resistance to benomyl and methotrexate

  1991cited by this paper
• Proteinase Production and Pathogenicity of Candida albicans

  1987cited by this paper
• Proteinase Production and Pathogenicity of Candida albicans

  1987cited by this paper
• A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

  1984cited by this paper
• Candida and candidosis

  1979cited by this paper

• Gene expression profiling of the carbon pathways and virulence factors of Candida albicans in different carbon sources.

  2026cites this paper
• Exporter Mdr1 as an importer is an achilles' heel for combating drug-resistant Candida.

  2026cites this paper
• Candida albicans: Insights into the Biology and Experimental Innovations of a Commonly Isolated Human Fungal Pathogen.

  2025cites this paper
• Mechanisms of resistance to cell wall and plasma membrane targeting antifungal drugs in Candida species isolated in Africa

  2025cites this paper
• Agrochemical fungicides as environmental drivers of antifungal resistance.

  2025cites this paper
• Sub-Therapeutic Concentrations of Hexetidine Induce Virulence Gene Expression in Candida albicans

  2025cites this paper
• In vitro inhibitory activity of Riparins against Candida spp. strains and in silico interaction with multi-drug-resistance proteins

  2024cites this paper
• Adaptation of Candida albicans to specific host environments by gain-of-function mutations in transcription factors

  2024cites this paper
• Azole Combinations and Multi-Targeting Drugs That Synergistically Inhibit Candidozyma auris

  2024cites this paper
• Role of ERG11 and MDR1 genes in cycloheximide and multidrug resistance in Candida species

  2024cites this paper
• Aneuploidy Mediates Rapid Adaptation to a Subinhibitory Amount of Fluconazole in Candida albicans

  2023cites this paper
• Role of bacterial efflux pump proteins in antibiotic resistance across microbial species.

  2023cites this paper
• Clorgyline Analogs Synergize with Azoles against Drug Efflux in Candida auris

  2023cites this paper
• Transcriptional Response of Candida auris to the Mrr1 Inducers Methylglyoxal and Benomyl

  2022cites this paper
• The Role of Glycoside Hydrolases in S. gordonii and C. albicans Interactions

  2022cites this paper
• Genomic Data Mining Reveals Abundant Uncharacterized Transporters in Coccidioides immitis and Coccidioides posadasii

  2022cites this paper
• Drug resistance in Candida albicans isolates and related changes in the structural domain of Mdr1 protein.

  2021cites this paper
• CRISPR-Based Genetic Manipulation of Candida Species: Historical Perspectives and Current Approaches

  2021cites this paper
• Novel ERG11 and TAC1b Mutations Associated with Azole Resistance in Candida auris

  2021cites this paper
• Fructose Induces Fluconazole Resistance in Candida albicans through Activation of Mdr1 and Cdr1 Transporters

  2021cites this paper
• A biological and genomic comparison of a drug-resistant and a drug-susceptible strain of Candida auris isolated from Beijing, China

  2021cites this paper
• Correction to: Germination of a Field: Women in Candida albicans Research

  2021cites this paper
• Germination of a Field: Women in Candida albicans Research

  2021cites this paper
• Emergence of Antifungal Azole Resistance in the Fungal Strains of Tinea corporis, Tinea capitis, Tinea cruris and Tinea pedis from the Locality of Southern Punjab, Pakistan

  2021cites this paper
• Bio- and Nanotechnology as the Key for Clinical Application of Salivary Peptide Histatin: A Necessary Advance

  2020cites this paper
• Early Onset and Late Onset of Neonatal Sepsis in a Tertiary Hospital, South-South, Nigeria

  2020cites this paper
• Análisis cuantitativo de la expresión de genes de resistencia a fluconazol en cepas de Candida albicans aisladas al ingreso de adultos mayores a una unidad de cuidados intensivos de Manizales, Colombia

  2020cites this paper
• Bio- and Nanotechnology as the Key for Clinical Application of Salivary Peptide Histatin: A Necessary Advance.

  2020cites this paper
• Antifungal Drug Resistance: Molecular Mechanisms in Candida albicans and Beyond.

  2020cites this paper
• Berberine reverses multidrug resistance in Candida albicans by hijacking the drug efflux pump Mdr1p

  2020influential citation
• An Investigation into Clinically Relevant Determinants of Azole Resistance in Candida albicans

  2019cites this paper
• Molecular and genetic basis of azole antifungal resistance in the opportunistic pathogenic fungus Candida albicans.

  2019cites this paper
• Trichophyton rubrum Azole Resistance Mediated by a New ABC Transporter, TruMDR3

  2019cites this paper
• Study of HST6 function in Candida albicans through the application of a CRISPR-Cas9 gene editing system

  2019cites this paper
• Pichia pastoris protease‐deficient and auxotrophic strains generated by a novel, user‐friendly vector toolbox for gene deletion

  2019cites this paper
• Targeting Candida spp. to develop antifungal agents.

  2018cites this paper
• Host-Pathogen Interactions Mediated by MDR Transporters in Fungi: As Pleiotropic as it Gets!

  2018cites this paper
• Using Yeast to Discover Inhibitors of Multidrug Efflux in Candida albicans

  2017cites this paper
• Parasex Generates Phenotypic Diversity de Novo and Impacts Drug Resistance and Virulence in Candida albicans

  2017cites this paper
• MDR1 and Its Regulation

  2017cites this paper
• Structure–Function Analyses of Multidrug Transporters

  2017cites this paper
• Isolation and characterisation of antifungal and antibacterial compounds from Combretum molle (Combretaceae) leaf extracts

  2017cites this paper
• Fluconazole resistance in Candida species: a current perspective

  2017cites this paper
• The development of fluconazole resistance in Candida albicans – an example of microevolution of a fungal pathogen

  2016cites this paper
• Quinone derivatives isolated from the endolichenic fungus Phialocephala fortinii are Mdr1 modulators that combat azole resistance in Candida albicans

  2016cites this paper
• A new rapid and efficient system with dominant selection developed to inactivate and conditionally express genes in Candida albicans

  2016influential citation
• A Combination Fluorescence Assay Demonstrates Increased Efflux Pump Activity as a Resistance Mechanism in Azole-Resistant Vaginal Candida albicans Isolates

  2016cites this paper
• Clotrimazole Drug Resistance in Candida glabrata Clinical Isolates Correlates with Increased Expression of the Drug:H+ Antiporters CgAqr1, CgTpo1_1, CgTpo3, and CgQdr2

  2016cites this paper
• Epidemiology and molecular mechanisms of antifungal resistance in Candida and Aspergillus

  2016cites this paper
• MFS transporters of Candida species and their role in clinical drug resistance.

  2016cites this paper
• Chemical Transformation of Candida albicans

  2015cites this paper
• Candida albicans mutant construction and characterization of selected virulence determinants.

  2015cites this paper
• MFS multidrug transporters in pathogenic fungi: do they have real clinical impact?

  2014cites this paper
• Antifungal Compound Mdr1p-Mediated Uptake of a Novel Multidrug-Resistant Transporter

  2014cites this paper
• Phenotypic and molecular genetic profiles of fluconazole and voriconazole sensitive versus resistant candida spp

  2014cites this paper
• Molecular genetic techniques for gene manipulation in Candida albicans

  2014cites this paper
• Multidrug resistance in fungi: regulation of transporter-encoding gene expression

  2014cites this paper
• Berberine inhibits fluphenazine-induced up-regulation of CDR1 in Candida albicans.

  2014cites this paper
• SAGA/ADA Complex Subunit Ada2 Is Required for Cap1- but Not Mrr1-Mediated Upregulation of the Candida albicans Multidrug Efflux Pump MDR1

  2014cites this paper
• New Clox Systems for Rapid and Efficient Gene Disruption in Candida albicans

  2014cites this paper
• Chromosome 5 Monosomy of Candida albicans Controls Susceptibility to Various Toxic Agents, Including Major Antifungals

  2013influential citation
• Candida parapsilosis Is a Significant Neonatal Pathogen: A Systematic Review and Meta-analysis

  2013cites this paper
• Multidrug-Resistant Transporter Mdr1p-Mediated Uptake of a Novel Antifungal Compound

  2013cites this paper
• Signal Transduction and Transcriptional Regulation Pathways Essential for Azole Resistance in Candida albicans

  2013influential citation
• Hijacking the Candida albicans Multidrug Resistant Transporter MDR1 to Potentiate Antifungal Activity

  2013cites this paper
• Functional Studies on the Histidine Kinase CaNik1p from Candida albicans

  2013cites this paper
• Gene deletion in Candida albicans wild-type strains using the SAT1-flipping strategy.

  2012cites this paper
• Deus ex Candida genetics: overcoming the hurdles for the development of a molecular toolbox in the CTG clade.

  2012cites this paper
• Curcumin acts synergistically with fluconazole to sensitize a clinical isolate of Candida albicans showing a MDR phenotype.

  2012cites this paper
• Population dynamics and the evolution of antifungal drug resistance in Candida albicans.

  2012cites this paper
• The fungicide fludioxonil antagonizes fluconazole activity in the human fungal pathogen Candida albicans.

  2012cites this paper
• In Vitro Evolutionary Dynamics of C. albicans during Adaptation to Fluocnazole

  2012cites this paper
• Inducible and Constitutive Activation of Two Polymorphic Promoter Alleles of the Candida albicans Multidrug Efflux Pump MDR1

  2012cites this paper
• The cellular and molecular defense mechanisms of the Candida yeasts against azole antifungal drugs.

  2012cites this paper
• Comparative Lipidomics of Azole Sensitive and Resistant Clinical Isolates of Candida albicans Reveals Unexpected Diversity in Molecular Lipid Imprints

  2011cites this paper
• Cassettes for PCR‐mediated gene tagging in Candida albicans utilizing nourseothricin resistance

  2011cites this paper
• Evolutionary Dynamics of Candida albicans during In Vitro Evolution

  2011cites this paper
• Regulation of Efflux Pump Expression and Drug Resistance by the Transcription Factors Mrr1, Upc2, and Cap1 in Candida albicans

  2011cites this paper
• Milestones in Candida albicans gene manipulation.

  2011influential citation
• Resistance to Antifungal Drugs

  2011cites this paper
• An MDR1 promoter allele with higher promoter activity is common in clinically isolated strains of Candida albicans

  2011cites this paper
• Difference in virulence between fluconazole‐susceptible and fluconazole‐resistant Candida albicans in a mouse model

  2011cites this paper
• Effect of fatty acids on hyphal growth in the pathogenic yeast Candida albicans

  2011cites this paper
• Identificação e análise de mutações no gene ERG11 de isolados de Candida susceptíveis e resistentes ao fluconazol

  2011cites this paper
• Functional analysis of Candida albicans genes encoding SH3-domain-containing proteins.

  2010cites this paper
• Antifungal curcumin induces reactive oxygen species and triggers an early apoptosis but prevents hyphae development by targeting the global repressor TUP1 in Candida albicans.

  2010cites this paper
• Genetic Dissection of Azole Resistance Mechanisms in Candida albicans and Their Validation in a Mouse Model of Disseminated Infection

  2010cites this paper
• The use of FLP-mediated recombination for the functional analysis of an effector gene family in the biotrophic smut fungus Ustilago maydis.

  2010cites this paper
• Application of the Saccharomyces cerevisiae FLP/FRT Recombination System in Filamentous Fungi for Marker Recycling and Construction of Knockout Strains Devoid of Heterologous Genes

  2010cites this paper
• Identification des réseaux transcriptionnnels de résistance aux antifongiques chez Candida albicans

  2010influential citation
• Regulation of multidrug resistance in pathogenic fungi.

  2010cites this paper
• The white cell pheromone response pathway in Candida albicans provides insights into the evolution of new signal transduction pathways

  2010cites this paper
• A CUG codon adapted two-hybrid system for the pathogenic fungus Candida albicans

  2010cites this paper
• Use of the Aspergillus oryzae actin gene promoter in a novel reporter system for exploring antifungal compounds and their target genes

  2010cites this paper
• Candida albicans AGE3, the Ortholog of the S. cerevisiae ARF-GAP-Encoding Gene GCS1, Is Required for Hyphal Growth and Drug Resistance

  2010cites this paper
• Efflux in Fungi: La Pièce de Résistance

  2009cites this paper
• Genetic transformation of Candida albicans.

  2009cites this paper
• Identification of Nile red as a fluorescent substrate of the Candida albicans ATP-binding cassette transporters Cdr1p and Cdr2p and the major facilitator superfamily transporter Mdr1p.

  2009cites this paper
• Efflux-Mediated Antifungal Drug Resistance

  2009cites this paper
• Novel subfamily of mitochondrial HMG box-containing proteins: functional analysis of Gcf1p from Candida albicans.

  2009cites this paper