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Single-Cell Omics Approaches in Plants

R. Kambale,M. H. Siddiqui,R. Muthurajan,H. Rahman

Published 2019 in Single-Cell Omics

ABSTRACT

Abstract Gene expression analysis in bulk samples containing different types of cells masks the properties of individual cell types because of averaging artifacts of the different cell types. With the advances in techniques for single-cell isolation, omics analysis of single-cells has become possible which helps in revealing the inherent properties of single-cells at high resolution. With the advent of next-generation sequencing platforms, single-cell RNA-sequencing protocols have been developed and are evolving day by day. Single-cell omics helps to identify expressed genes, proteins, and metabolites in individual cells of a given population. Emerging technologies for single-cell omics holds the promise not only to revolutionize our understanding of biological processes but also to help in determining the role of different cell types in plants. This will elucidate very precisely the molecular basis of plant growth and development, as well as the role of different cell types in providing tolerance against various environmental stresses.

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