Multiplex Genome Engineering Using CRISPR/Cas Systems

No abstract is available for this paper.

• Publication year

  2013

• Venue

  Science

• Publication date

  2013-02-01

• Fields of study

  Biology, Medicine, Engineering

• Identifiers
  DOI 10.1126/science.1231143PMID 23287718PMCID PMC3795411
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Ieee Transactions On Cybernetics

  2015cited by this paper
• RNA-Guided Human Genome Engineering via Cas9

  2013cited by this paper
• CRISPR: new horizons in phage resistance and strain identification.

  2012cited by this paper
• A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

  2012cited by this paper
• Cleavage of Phage DNA by the Streptococcus thermophilus CRISPR3-Cas System

  2012cited by this paper
• Cas9–crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

  2012cited by this paper
• FLASH Assembly of TALENs Enables High-Throughput Genome Editing

  2012cited by this paper
• Tracking genome engineering outcome at individual DNA breakpoints

  2011cited by this paper
• Evolution and classification of the CRISPR–Cas systems

  2011cited by this paper
• CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III

  2011cited by this paper
• The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli

  2011cited by this paper
• CRISPR-Cas systems in bacteria and archaea: versatile small RNAs for adaptive defense and regulation.

  2011cited by this paper
• Targeted Genome Editing Across Species Using ZFNs and TALENs

  2011cited by this paper
• A TALE nuclease architecture for efficient genome editing

  2011cited by this paper
• Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription.

  2011cited by this paper
• The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA

  2010cited by this paper
• CRISPR/Cas system and its role in phage-bacteria interactions.

  2010cited by this paper
• Selection-Free Zinc-Finger Nuclease Engineering by Context-Dependent Assembly (CoDA)

  2010cited by this paper
• Targeting DNA Double-Strand Breaks with TAL Effector Nucleases

  2010cited by this paper
• CRISPR/Cas, the Immune System of Bacteria and Archaea

  2010cited by this paper
• A rapid and general assay for monitoring endogenous gene modification.

  2010cited by this paper
• Small RNAs as Guardians of the Genome

  2009cited by this paper
• A three-dimensional view of the molecular machinery of RNA interference

  2009cited by this paper
• A Simple Cipher Governs DNA Recognition by TAL Effectors

  2009cited by this paper
• Genome engineering

  2009cited by this paper
• Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors

  2009cited by this paper
• Short motif sequences determine the targets of the prokaryotic CRISPR defence system.

  2009cited by this paper
• Small RNAs as guardians of the genome.

  2009cited by this paper
• Short motif sequences determine the targets of the prokaryotic CRISPR defence system.

  2009cited by this paper
• Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes

  2008cited by this paper
• Phage Response to CRISPR-Encoded Resistance in Streptococcus thermophilus

  2007cited by this paper
• An improved zinc-finger nuclease architecture for highly specific genome editing

  2007cited by this paper
• Homing endonuclease structure and function

  2005cited by this paper
• IL-13受体α2降低血吸虫病肉芽肿的炎症反应并延长宿主存活时间[英]/Mentink-Kane MM,Cheever AW，Thompson RW，et al//Proc Natl Acad Sci U S A

  2005cited by this paper
• Mechanisms of gene silencing by double-stranded RNA

  2004cited by this paper
• Chimeric Nucleases Stimulate Gene Targeting in Human Cells

  2003cited by this paper
• Genetics

  1981cited by this paper
• Materials and methods.

  1973cited by this paper
• Experiments in molecular genetics

  1972cited by this paper
• Microbiology

  1950cited by this paper
• FLAsH assembly of TALeNs for high-throughput genome editing

  year unknowncited by this paper

• RPA-independent activation of the ATR/CHK1 pathway.

  2026cites this paper
• Engineered Un1Cas12f1 for multiplex genome editing with enhanced activity and targeting scope.

  2026cites this paper
• Genome editing of medicinal plants: Advances, challenges, and prospects.

  2026cites this paper
• Mouse and human T cell Cas9-RNP/nucleofection-mediated gene-editing.

  2026cites this paper
• Oridonin Ameliorates Alzheimer's Disease-Like Pathology in Male Mice Through Inhibition of Receptor-Interacting Protein Kinase 1.

  2026cites this paper
• Multiplex gene editing drives revolution in crop breeding: overlaid editing of multiple genes and customization of complex traits

  2026cites this paper
• Programmable adenine base editing in cyanobacteria using an engineered TadA-Cas9 fusion.

  2026cites this paper
• Generation of a Brachyury reporter cell line (BJNhem20 Brachyury (TBXT)-2A-EGFP) in human embryonic stem cells using CRISPR-Cas9 gene targeting.

  2026cites this paper
• Engineering the MmeFz2-ωRNA system for efficient genome editing through an integrated computational-experimental framework

  2026cites this paper
• Bio-orthogonal chemistry-based strategy to Turn-OFF CRISPR-Cas9 activity in solution and live cells

  2026cites this paper
• Generation of a SV2A knockout human embryonic stem cell line by CRISPR/Cas9 system.

  2026cites this paper
• Therapeutic Gene Editing: DNA Repair Pathways, Emerging Editors, and Clinical Progress

  2026cites this paper
• Potential of CRISPR/Cas9 Genome Engineering for Biofuel Production From Economically Relevant Algae

  2026cites this paper
• Viral genome editing methods and applications in the CRISPR era

  2026cites this paper
• Engineered miniature CRISPR activation system promotes dodecanedioic acid production in Candida viswanathii

  2026cites this paper
• The Zinc-Finger Protein POGZ Associates with Polycomb Repressive Complex 1 to Regulate Bone Morphogenetic Protein Signaling During Neuronal Differentiation

  2026cites this paper
• Engineering delivery platforms for CRISPR-Cas and their applications in healthcare, agriculture and beyond

  2026cites this paper
• Enhanced editing of Bifidobacterium lactis using the endogenous Type I-G CRISPR-Cas system

  2026cites this paper
• Emerging DNA- and RNA-based therapeutic strategies in hepatocellular carcinoma: A molecular approach to precision medicine

  2026cites this paper
• Gene Modification: Exploring the potential in treating kidney diseases.

  2026cites this paper
• New genomic and proteomic biomarker discovery in cancer: revolutionizing diagnosis and prognostication

  2026cites this paper
• CRISPR/Cas9 and reproductive failure: applications, ethical challenges, and future perspectives in human germline genome editing.

  2026cites this paper
• Strawberry Notch 1 Acts as a Transcriptional Regulator Driving Oncogenic Programs in Liver Carcinogenesis.

  2026cites this paper
• SETDB1/ATF7IP regulate the precise genome engineering of HUSH-regulated genes

  2026cites this paper
• The application of CRISPR-Cas9 system in brain diseases.

  2026cites this paper
• Evolution and advancements of techniques in generating transgenic animals: from random integration to targeted precision

  2026cites this paper
• Mechanisms linking cytoplasmic decay of translation-defective mRNA to transcriptional adaptation.

  2026cites this paper
• Biosensor Technologies for Avian Influenza Detection: A New Frontier in Rapid Diagnostics for HPAI.

  2026cites this paper
• Quantitative analysis of small RNA pseudouridylation reveals interplay of PUS enzymes in tRNA anticodon stem-loop.

  2026cites this paper
• CRISPR as a therapeutic tool for inherited retinal degenerations: Advances, challenges, and future directions.

  2026cites this paper
• Engineering Pichia pastoris Strains Using CRISPR/Cas9 Technologies: The Basic Protocol.

  2026cites this paper
• Programmable molecular microscopy: CRISPR/Cas fluorescent probes revolutionizing spatiotemporal genomic imaging

  2026cites this paper
• Stage- and tissue-specific gene editing using 4-OHT–inducible Cas9 in whole organism

  2026cites this paper
• Engineering Mutation Clones in Mammalian Cells with CRISPR/Cas9.

  2026cites this paper
• The Chimeric Nuclease SpRYc Exhibits Highly Variable Performance Across Biological Systems

  2026cites this paper
• CRISPR-Cas12a biosensing technology advances and applications in precision diagnostics and cancer research.

  2026cites this paper
• Single-cell multiplex approaches deeply map ON-target CRISPR-genotoxicity and reveal its mitigation by palbociclib and long-term engraftment

  2026cites this paper
• Salinity responses and tolerance mechanisms in sugarcane (Saccharum officinarum L.): Recent updates and future perspectives

  2026cites this paper
• nf-core/crisprseq: a versatile pipeline for comprehensive analysis of CRISPR gene editing and screening assays

  2026cites this paper
• Modular gene tagging in C. elegans

  2026cites this paper
• Spatially confined genome editing enables localized combination immunotherapy

  2026cites this paper
• Persistent enhancement of intrinsic neuronal excitability induced by transient divalent cation depletion

  2026cites this paper
• Prokaryotic Molecular Defense Mechanisms and Their Potential Applications in Cancer Biology: A Special Consideration for Cyanobacterial Systems

  2026cites this paper
• GenAI-Net: A Generative AI Framework for Automated Biomolecular Network Design

  2026cites this paper
• Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications

  2026cites this paper
• Identification of MKRN1 as a key modulator of the p53-MDM2 feedback loop.

  2026cites this paper
• Atlas-guided discovery of transcription factors for T cell programming.

  2026cites this paper
• Programmable genome editing in human cells using RNA-guided bridge recombinases.

  2026cites this paper
• Amplified ferroptosis and immunomodulation triggered by NIR-II photothermal-controllable CRISPR/Cas9 nanoplatform to treat osteosarcoma and prevent postsurgical implant-associated infections.

  2026cites this paper
• Pharmaceutical protein production by transgenic chickens: several viewpoints towards the next stage.

  2026cites this paper
• Proteomics and phosphoproteomics of human colorectal cancer cells lacking a specific kinase activity reveal kinase-specific compensatory responses

  2026cites this paper
• Engineering high-efficiency mutants of the transposase ISDra2 TnpB via protein-generative models and energy-optimized design

  2026cites this paper
• Comparison of CRISPR-Cas-based knockdown of endogenous mRNA in sensory neurons

  2026cites this paper
• The Algal Carbon Diet: Repurposing Volatile Fatty Acids into Sustainable Lipids

  2026cites this paper
• dHyperCas12a enables multiplexed CRISPRi screens.

  2026cites this paper
• Evolution of Prime Editing: Enhancing Efficiency and Expanding Capacity.

  2026cites this paper
• A CRISPR/Cas9-regulated dual-ring topological allosteric probe for detection of the EGFR L858R resistance mutation in CTCs.

  2026cites this paper
• An orthogonal CRISPR/Cpf1 platform for precise spatiotemporal gene regulation and osteoporotic fracture repair.

  2026cites this paper
• Advances in rapid on-site detection techniques for food safety and authenticity.

  2026cites this paper
• A highly sensitive universal modularized CRISPR/Cas14a system for antibiotic sensing

  2026cites this paper
• Split technology in CRISPR-Cas system for precision genome manipulation and diagnostics

  2026cites this paper
• CRISPR genome editing in plants without tissue culture.

  2026cites this paper
• Metabolic basis of the astrocyte-synapse interaction governs dopaminergic-motor connection

  2026cites this paper
• Enhancing CRISPR Homology Directed Repair in IAL-PiD2 Insect Cells via Reagent Delivery Optimization and Cell Synchronization.

  2026cites this paper
• Cloning-Free Genome Editing by CRISPR/T7RNAP/Cas9 in Trypanosoma cruzi.

  2026cites this paper
• Emerging Targeted Therapies for Inherited Cardiomyopathies and Arrhythmias.

  2026cites this paper
• Divalent cation depletion enhances neuronal excitability through CaSR-dependent modulation of threshold channels

  2026cites this paper
• Efficient Fluorescent Labeling of Human Trophoblast Stem Cells via a CRISPR/Cas9-Mediated Knock-In Approach in a Safe Harbor Locus

  2026cites this paper
• Generation of IL17RB Knockout Cell Lines Using CRISPR/Cas9-Based Genome Editing.

  2026cites this paper
• Establishment of a Novel CRISPR/Cas9-Based Multiplex Editing System in the Citrus Postharvest Pathogen Penicillium digitatum.

  2026cites this paper
• Rising Star Engineering the Genome for Curative Futures.

  2026cites this paper
• Remote control of bacterial gene expression: Optogenetics, electrogenetics, sonogenetics and magnetogenetics

  2026cites this paper
• Stem cell-based and gene editing strategies in the treatment of congenital and acquired sensorineural hearing loss: a narrative review.

  2026cites this paper
• Intrathecal CRISPR-edited allogeneic IL-13Rα2 CAR T Cells for recurrent high-grade Glioma: preclinical characterization and phase I trial

  2026cites this paper
• Identification of novel inhibitors of Mycobacterium smegmatis growth through genome-wide overexpression of Cluster P3 mycobacteriophage Xavia genes

  2026cites this paper
• The role of bacteriophages and CRISPR-Cas in combating multidrug-resistant bacteria

  2026cites this paper
• CRISPR-Cas9 technology: a breakthrough in cancer gene therapy

  2026cites this paper
• Hyaluronic Acid‐Coated DNA Nanoflowers as Smart Nanocarriers for Deep Tumor Penetration and Enhanced Gene Therapy in Pancreatic Cancer

  2026cites this paper
• A Bst-driven Cas12a cascade amplification strategy for microRNA detection.

  2026cites this paper
• Application of CRISPR/Cas9 in Reconstructing Bacterial Deoxyribonucleic Acid for the Production of Biologically Active Compounds

  2026cites this paper
• Mapping the science–technology translational landscape in biomedicine

  2026cites this paper
• Non-Viral CRISPR carriers: transient delivery with lasting effects

  2026cites this paper
• Advances in lipid nanoparticles delivering genetic medicines for solid cancers

  2026cites this paper
• Chronic In Vivo CRISPR-Cas Genome Editing: Challenges, Long-Term Safety, and Outlook

  2026cites this paper
• TriCON: A Carbon-Based Triple-Modal Nanoplatform for Pancreatic Cancer Therapy.

  2026cites this paper
• Plant natural product biosynthesis through metabolon engineering.

  2026cites this paper
• Exploiting the Endogenous Type II‐A CRISPR‐Cas System for Functional Engineering of Probiotic Lacticaseibacillus rhamnosus GG

  2026cites this paper
• HLA‐Knockout: Enabling Allele‐Specific Knockout of HLA Class I Genes for Immunogenic Engineering

  2026cites this paper
• Comprehensive assessment of activity, specificity, and safety of hypercompact TnpB systems for gene editing

  2026cites this paper
• Implication of plant growth promoting microorganisms and nanofertilizer in millets: a review on growth promotion and crop improvement

  2026cites this paper
• PEGylated DNA Helper Molecule-Assisted Self-Assembly of Polyelectrolyte Complex Micelles.

  2026cites this paper
• Gene Editing and Transformation Strategies for Engineering Drought‐Tolerant Legumes

  2026cites this paper
• [Research progress and prospects in CRISPR-Cas9 gene editing in sorghum].

  2026cites this paper
• Precise probiotic therapy: Advances, bottlenecks, and the road to microbiome-informed nutrition

  2026cites this paper
• Impacts of DNA Supercoiling on the Sequence-Dependent Nuclease Activity of CRISPR-Cas9 with Truncated Guides.

  2026cites this paper
• Safe and Efficient CRISPR Genome Editing of Primary Human T Cells Using a Droplet-Based Cell Mechanoporation Platform.

  2026cites this paper
• Optimizing prime editing: Advances in efficiency enhancement.

  2026cites this paper
• Abasic CRISPR RNAs inherently harness fidelity of SpCas9 for genome editing.

  2026cites this paper
• LncRNAs in Ovarian Cancer: Emerging Insights and Future Perspectives in Tumor Biology and Clinical Applications

  2026cites this paper
• Conformation-programmed DNA computing.

  2026cites this paper