Ku-Dependent Nonhomologous DNA End Joining inXenopus Egg Extracts

No abstract is available for this paper.

• Publication year

  1999

• Venue

  Molecular and Cellular Biology

• Publication date

  1999-04-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1128/MCB.19.4.2585PMID 10082524PMCID PMC84051
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Implication of DNA-dependent protein kinase in an early, essential, local phosphorylation event during end-joining of DNA double-strand breaks in vitro.

  1998cited by this paper
• Interaction of Ku protein and DNA-dependent protein kinase catalytic subunit with nucleic acids.

  1998cited by this paper
• Yeast Ku as a regulator of chromosomal DNA end structure.

  1998cited by this paper
• mRNA encoding the catalytic subunit of DNA-dependent protein kinase is widely expressed in Xenopus cells.

  1997cited by this paper
• V(D)J recombination: in vitro coding joint formation

  1997cited by this paper
• Interaction of DNA‐dependent protein kinase with DNA and with Ku: biochemical and atomic‐force microscopy studies

  1997cited by this paper
• Double strand break repair.

  1997cited by this paper
• Ku proteins join DNA fragments as shown by atomic force microscopy.

  1997cited by this paper
• End-joining of Free Radical-mediated DNA Double-strand Breaks in Vitro Is Blocked by the Kinase Inhibitor Wortmannin at a Step Preceding Removal of Damaged 3′ Termini*

  1996cited by this paper
• In vitro V(D)J recombination: signal joint formation.

  1996cited by this paper
• The DNA-activated protein kinase -- DNA-PK.

  1996influential reference
• Identification of a Saccharomyces cerevisiae Ku80 homologue: roles in DNA double strand break rejoining and in telomeric maintenance.

  1996cited by this paper
• Dramatic changes in the ratio of homologous recombination to nonhomologous DNA-end joining in oocytes and early embryos of Xenopus laevis.

  1996cited by this paper
• Mechanisms of nonhomologous DNA end-joining in frogs, mice and men.

  1996cited by this paper
• DNA double-strand break repair and V(D)J recombination: involvement of DNA-PK.

  1995cited by this paper
• The Xenopus 9 bp ribosomal terminator (T3 box) is a pause signal for the RNA polymerase I elongation complex.

  1995cited by this paper
• Defective DNA-dependent protein kinase activity is linked to V(D)J recombination and DNA repair defects associated with the murine scid mutation.

  1995cited by this paper
• DNA-dependent protein kinase catalytic subunit: a relative of phosphatidylinositol 3-kinase and the ataxia telangiectasia gene product.

  1995cited by this paper
• DNA-dependent kinase (p350) as a candidate gene for the murine SCID defect

  1995cited by this paper
• Ku80: product of the XRCC5 gene and its role in DNA repair and V(D)J recombination.

  1994cited by this paper
• Characterization of DNA end joining in a mammalian cell nuclear extract: junction formation is accompanied by nucleotide loss, which is limited and uniform but not site specific

  1994cited by this paper
• Nonhomologous recombination in human cells

  1994cited by this paper
• Mechanisms of overlap formation in nonhomologous DNA end joining

  1994cited by this paper
• Restoration of X-ray resistance and V(D)J recombination in mutant cells by Ku cDNA.

  1994cited by this paper
• The DNA-dependent protein kinase: requirement for DNA ends and association with Ku antigen.

  1993cited by this paper
• Homologous and illegitimate recombination in developing Xenopus oocytes and eggs

  1993cited by this paper
• Use of DNA end joining activity of a Xenopus laevis egg extract for construction of deletions and expression vectors for HIV-1 Tat and Rev proteins.

  1993influential reference
• Antibodies to the p70/p80 (Ku) antigens in systemic lupus erythematosus.

  1992cited by this paper
• Antibodies to the p70/p80 (Ku) antigens in systemic lupus erythematosus.

  1992cited by this paper
• The nuclear serine/threonine protein kinase DNA-PK.

  1992influential reference
• Antigenic determinants of the 70-kDa subunit of the Ku autoantigen.

  1992influential reference
• Activation of a system for the joining of nonhomologous DNA ends during Xenopus egg maturation

  1992cited by this paper
• Epitopes of the p70 and p80 (Ku) lupus autoantigens.

  1991cited by this paper
• Purification and characterization of proximal sequence element-binding protein 1, a transcription activating protein related to Ku and TREF that binds the proximal sequence element of the human U1 promoter.

  1990cited by this paper
• A DNA-activated protein kinase from HeLa cell nuclei

  1990cited by this paper
• A novel pathway of DNA end-to-end joining.

  1990cited by this paper
• The defect in murine severe combined immune deficiency: joining of signal sequences but not coding segments in V(D)J recombination.

  1988cited by this paper
• Joining of nonhomologous DNA double strand breaks in vitro.

  1988cited by this paper
• Mechanism of interaction between Ku protein and DNA.

  1986cited by this paper
• Use of monoclonal antibodies for the characterization of novel DNA- binding proteins recognized by human autoimmune sera

  1985cited by this paper
• The use of Xenopus oocytes for the expression of cloned genes.

  1983cited by this paper

• Measuring protein stoichiometry with single-molecule imaging in Xenopus egg extracts.

  2024cites this paper
• Holding it together: DNA end synapsis during non-homologous end joining.

  2023cites this paper
• Structural role for DNA Ligase IV in promoting the fidelity of non-homologous end joining

  2022cites this paper
• Repair of DNA Double-Strand Breaks by the Nonhomologous End Joining Pathway.

  2021cites this paper
• Novel gross deletion at the LHX4 gene locus in a child with growth hormone deficiency.

  2021cites this paper
• Mechanistic Insights From Single-Molecule Studies of Repair of Double Strand Breaks

  2021cites this paper
• Polθ promotes the repair of 5′-DNA-protein crosslinks by microhomology-mediated end-joining

  2021influential citation
• A mechanism to minimize errors during non-homologous end joining

  2019cites this paper
• A Mechanism to Minimize Errors during Non-homologous End Joining.

  2019influential citation
• A Single XLF Dimer Bridges DNA Ends During Non-Homologous End Joining

  2018cites this paper
• The Human T-Cell Leukemia Virus Type 1 Basic Leucine Zipper Factor Attenuates Repair of Double-Stranded DNA Breaks via Nonhomologous End Joining

  2018cites this paper
• The capacity of oocytes for DNA repair

  2018cites this paper
• Single-Molecule Imaging of DNA Organization and Repair

  2017influential citation
• Ensemble and Single-Molecule Analysis of Non-Homologous End Joining in Frog Egg Extracts.

  2017influential citation
• Xenopus egg extract: A powerful tool to study genome maintenance mechanisms.

  2017cites this paper
• Cip29 is phosphorylated following activation of the DNA damage response in Xenopus egg extracts

  2017cites this paper
• Two-Stage Synapsis of DNA Ends during Non-homologous End Joining.

  2016influential citation
• The structure of ends determines the pathway choice and Mre11 nuclease dependency of DNA double-strand break repair

  2016cites this paper
• Non-homologous end joining repair in Xenopus egg extract

  2016cites this paper
• Award Number : W 81 XWH-13-1-0063 TITLE : A New Cell-Free System to Study BRCA 1 Function

  2016cites this paper
• Functional assessment for elimination of mismatches in nuclear and whole cell extracts obtained from mouse and human blastocysts

  2016cites this paper
• Analysis of double-strand break repair by nonhomologous DNA end joining in cell-free extracts from mammalian cells.

  2014cites this paper
• The down-regulation of Ku70, DNA-PKcs, and Parp-1 in mammalian cell lines

  2012cites this paper
• Mechanistic analysis of Xenopus EXO1's function in 5′-strand resection at DNA double-strand breaks

  2011cites this paper
• Replication protein A promotes 5′→3′ end processing during homology-dependent DNA double-strand break repair

  2011cites this paper
• The Mre11/Rad50/Nbs1 complex functions in resection-based DNA end joining in Xenopus laevis

  2009cites this paper
• In vitro non-homologous DNA end joining assays--the 20th anniversary.

  2009cites this paper
• Origin-dependent initiation of DNA replication within telomeric sequences

  2009cites this paper
• Ku80 removal from DNA through double strand break–induced ubiquitylation

  2008influential citation
• Is NHEJ a tumor suppressor or an aging suppressor?

  2008cites this paper
• Functional analysis of two novel DNA repair factors, Metnase and Pso4

  2008cites this paper
• Ku Heterodimer-Independent End Joining in Trypanosoma brucei Cell Extracts Relies upon Sequence Microhomology

  2007influential citation
• The mutagenic potential of non-homologous end joining in the absence of the NHEJ core factors Ku70/80, DNA-PKcs and XRCC4-LigIV.

  2007cites this paper
• Probing the nanoscale viscoelasticity of intracellular fluids in living cells.

  2007cites this paper
• Saccharomyces cerevisiae Sae2- and Tel1-Dependent Single-Strand DNA Formation at DNA Break Promotes Microhomology-Mediated End Joining

  2007cites this paper
• DNA end joining activity is reduced in Alzheimer's disease.

  2006cites this paper
• Assembly of nuclear matrix–bound protein complexes involved in non‐homologous end joining is induced by inhibition of DNA topoisomerase II

  2006influential citation
• Mechanistic Analysis of a DNA End Processing Pathway Mediated by the Xenopus Werner Syndrome Protein*

  2006cites this paper
• Pathways of DNA Double-Strand Break Repair in Mammalian Cells after Ionizing Radiation

  2005cites this paper
• Mechanisms of Non-Homologous DNA End Joining:Aspects of In Vitro Assays

  2005cites this paper
• Structural Aspects of Ku and the DNA-Dependent Protein Kinase Complex

  2005cites this paper
• The Role of the BRCA1 Tumor Suppressor in DNA Double-Strand Break Repair

  2005cites this paper
• Analysis of the Xenopus Werner syndrome protein in DNA double-strand break repair

  2005influential citation
• Ionizing radiation and genetic risks XIV. Potential research directions in the post-genome era based on knowledge of repair of radiation-induced DNA double-strand breaks in mammalian somatic cells and the origin of deletions associated with human genomic disorders.

  2005cites this paper
• Repair of double-strand breaks by nonhomologous end joining in the absence of Mre11

  2005influential citation
• The Role of the BRCA 1 Tumor Suppressor in DNA Double-Strand Break Repair

  2005cites this paper
• DNA ligase III as a candidate component of backup pathways of nonhomologous end joining.

  2005cites this paper
• Inhibition of Non-Homologous End Joining and integration of DNA upon transformation of Rhizopus oryzae

  2005cites this paper
• A Rad50-dependent pathway of DNA repair is deficient in Fanconi anemia fibroblasts.

  2004cites this paper
• DNA double strand break repair in human bladder cancer is error prone and involves microhomology-associated end-joining.

  2004cites this paper
• High frequency of nucleotide misincorporations upon the processing of double-strand breaks.

  2004cites this paper
• Backup pathways of NHEJ are suppressed by DNA‐PK

  2004cites this paper
• Xenopus Cds1 Is Regulated by DNA-Dependent Protein Kinase and ATR during the Cell Cycle Checkpoint Response to Double-Stranded DNA Ends

  2004cites this paper
• High G/C content of cohesive overhangs renders DNA end joining Ku-independent.

  2004cites this paper
• Nonhomologous end-joining in a cell-free extract from the cultured silkworm cell line BmN4

  2004cites this paper
• The non‐homologous end‐joining pathway is not involved in the radiosensitization of mammalian cells by heat shock

  2003cites this paper
• Non-homologous DNA end joining.

  2003cites this paper
• Regulation and mechanisms of mammalian double-strand break repair

  2003cites this paper
• DNA end sequestration by DNA‐dependent protein kinase and end joining of sterically constrained substrates in whole‐cell extracts

  2003influential citation
• Biochemical evidence for Ku-independent backup pathways of NHEJ.

  2003influential citation
• Yeast Mre11 and Rad1 Proteins Define a Ku-Independent Mechanism To Repair Double-Strand Breaks Lacking Overlapping End Sequences

  2003cites this paper
• Joining of DNA ends bearing non-matching 3'-overhangs.

  2002cites this paper
• Ku and the Stability of the Genome

  2002cites this paper
• DNA‐Dependent Protein Kinase (DNA‐PK)

  2002cites this paper
• Reconstitution of the mammalian DNA double-strand break end-joining reaction reveals a requirement for an Mre11/Rad50/NBS1-containing fraction.

  2002cites this paper
• Non‐homologous DNA end joining in the mature rat brain

  2002influential citation
• Accurate in Vitro End Joining of a DNA Double Strand Break with Partially Cohesive 3′-Overhangs and 3′-Phosphoglycolate Termini

  2001cites this paper
• The role of DNA polymerase activity in human non-homologous end joining.

  2001cites this paper
• RPA facilitates rejoining of DNA double-strand breaks in an in vitro assay utilizing genomic DNA as substrate

  2001cites this paper
• Expression of ATM in ataxia telangiectasia fibroblasts rescues defects in DNA double‐strand break repair in nuclear extracts

  2001cites this paper
• Direct transfer of Ku between DNA molecules with nonhomologous ends.

  2001cites this paper
• Nonhomologous DNA end joining in cell-free systems.

  2001cites this paper
• The influence of DNA double-strand break structure on end-joining in human cells.

  2001cites this paper
• Growing dictyate oocytes, but not early preimplantation embryos, of the mouse display high levels of DNA homologous recombination by single-strand annealing and lack DNA nonhomologous end joining.

  2001influential citation
• DNA Ligase IV and XRCC4 Form a Stable Mixed Tetramer That Functions Synergistically with Other Repair Factors in a Cell-free End-joining System*

  2000cites this paper
• Response of Xenopus Cds1 in cell-free extracts to DNA templates with double-stranded ends.

  2000cites this paper
• DNA double-strand break repair in cell-free extracts from Ku80-deficient cells: implications for Ku serving as an alignment factor in non-homologous DNA end joining

  2000cites this paper
• Ionizing radiation and genetic risks. XIII. Summary and synthesis of papers VI to XII and estimates of genetic risks in the year 2000.

  2000cites this paper
• DNA damage-induced cell cycle checkpoints and DNA strand break repair in development and tumorigenesis

  1999cites this paper
• Geometry of a complex formed by double strand break repair proteins at a single DNA end: recruitment of DNA-PKcs induces inward translocation of Ku protein.

  1999cites this paper