Incorporation of reporter molecule-labeled nucleotides by DNA polymerases. II. High-density labeling of natural DNA.

No abstract is available for this paper.

• Publication year

  2003

• Venue

  Nucleic Acids Research

• Publication date

  2003-05-15

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1093/NAR/GKG371PMID 12736314PMCID PMC156052
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Progress towards single-molecule sequencing: enzymatic synthesis of nucleotide-specifically labeled DNA.

  2001influential reference
• Fluorescent high-density labeling of DNA: error-free substitution for a normal nucleotide.

  2001cited by this paper
• In vitro replication slippage by DNA polymerases from thermophilic organisms.

  2001cited by this paper
• Towards a general procedure for sequencing single DNA molecules.

  2001cited by this paper
• Enhancing the catalytic repertoire of nucleic acids. II. Simultaneous incorporation of amino and imidazolyl functionalities by two modified triphosphates during PCR.

  2001cited by this paper
• Enhancing the catalytic repertoire of nucleic acids: a systematic study of linker length and rigidity.

  2001cited by this paper
• Identification of hydrogen bonds between Escherichia coli DNA polymerase I (Klenow fragment) and the minor groove of DNA by amino acid substitution of the polymerase and atomic substitution of the DNA.

  2001cited by this paper
• DNA polymerase switching: II. Replication factor C abrogates primer synthesis by DNA polymerase alpha at a critical length.

  2000cited by this paper
• Fold-back structures at the distal end influence DNA slippage at the proximal end during mononucleotide repeat expansions.

  1999cited by this paper
• Fluorescence correlation spectroscopy of enzymatic DNA polymerization.

  1998cited by this paper
• DNA polymerases on the move

  1998cited by this paper
• Molecular mechanism controlling the incorporation of fluorescent nucleotides into DNA by PCR.

  1997cited by this paper
• Stability, specificity and fluorescence brightness of multiply-labeled fluorescent DNA probes.

  1997cited by this paper
• Template-directed dye-terminator incorporation (TDI) assay: a homogeneous DNA diagnostic method based on fluorescence resonance energy transfer.

  1997cited by this paper
• Fractals in Biology and Medicine

  1994cited by this paper
• Directly labeled DNA probes using fluorescent nucleotides with different length linkers.

  1994cited by this paper
• Cyanine dye dUTP analogs for enzymatic labeling of DNA probes.

  1994influential reference
• Assay of DNA denaturation by polymerase chain reaction-driven fluorescent label incorporation and fluorescence resonance energy transfer.

  1994cited by this paper
• Sensitive detection of RNAs in single cells by flow cytometry [published erratum appears in Nucleic Acids Res 1992 Oct 25;20(20): 5518]

  1992cited by this paper
• Detection of in vivo-induced IL-1 mRNA in murine cells by flow cytometry (FC) and fluorescent in situ hybridization (FISH).

  1992cited by this paper
• Localizing DNA and RNA within nuclei and chromosomes by fluorescence in situ hybridization.

  1991cited by this paper
• Producing single-stranded DNA probes with the Taq DNA polymerase: a high yield protocol.

  1991cited by this paper
• Non-radioactive labeling and detection of nucleic acids. II. Optimization of the digoxigenin system.

  1990cited by this paper
• Oligonucleotides, part 5+: synthesis and fluorescence studies of DNA oligomers d(AT)5 containing adenines covalently linked at C-8 with dansyl fluorophore.

  1990cited by this paper
• Detection of DNA targets with biotinylated and fluoresceinated RNA probes. Effects of the extent of derivitization on detection sensitivity.

  1989cited by this paper
• Telomeric DNA dimerizes by formation of guanine tetrads between hairpin loops

  1989cited by this paper
• High-speed DNA sequencing: an approach based upon fluorescence detection of single molecules.

  1989cited by this paper
• Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries

  1988cited by this paper
• A system for rapid DNA sequencing with fluorescent chain-terminating dideoxynucleotides.

  1987cited by this paper
• Novel biotinylated nucleotide--analogs for labeling and colorimetric detection of DNA.

  1987cited by this paper
• Fluorescence detection in automated DNA sequence analysis

  1986cited by this paper

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  2018influential citation
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  2018cites this paper
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  2018cites this paper
• Enzymatic Synthesis Using Polymerases of Modified Nucleic Acids and Genes

  2018cites this paper
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  2018cites this paper
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  2017cites this paper
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• The 5-chlorouracil:7-deazaadenine base pair as an alternative to the dT:dA base pair.

  2016cites this paper
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  2016cites this paper
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