How the ribosome hands the A-site tRNA to the P site during EF-G–catalyzed translocation

No abstract is available for this paper.

• Publication year

  2014

• Venue

  Science

• Publication date

  2014-09-05

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1126/science.1255030PMID 25190797PMCID PMC4242719
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• GTP hydrolysis by EF‐G synchronizes tRNA movement on small and large ribosomal subunits

  2014cited by this paper
• Supplemental Information

  2013cited by this paper
• Dual use of GTP hydrolysis by elongation factor G on the ribosome

  2013cited by this paper
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  1995cited by this paper
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  1989cited by this paper
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  1978cited by this paper
• Supporting Online Material Materials and Methods Figs. S1 to S3 Tables S1 and S2 References Movie S1 the Structure of the Ribosome with Elongation Factor G Trapped in the Posttranslocational State

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