The central role of protein S12 in organizing the structure of the decoding site of the ribosome

Structural studies on the ribosome have provided tremendous insights into how cognate codon–anticodon interactions in the decoding center lead to tRNA selection and, ultimately, to peptide chain elongation. Here, in the genetically and structurally tractable organism Thermus thermophilus, the authors isolate and identify streptomycin-dependent (SmD) ribosomal variants, as well as streptomycin-independent (SmI) revertants, with mutations affecting ribosomal protein S12 and the 16S rRNA, respectively. Single-molecule FRET and structural studies reveal here how these decoding-site proximal mutations can impact the ability of ribosomes to reach the GTPase-activated state required for progression in tRNA selection.

• Publication year

  2013

• Venue

  RNA: A publication of the RNA Society

• Publication date

  2013-12-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1261/rna.040030.113PMID 24152548PMCID 3884664
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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