The 70-kDa peroxisomal membrane protein is a member of the Mdr (P-glycoprotein)-related ATP-binding protein superfamily.

The 70-kDa peroxisomal membrane protein (PMP70) is one of the major integral membrane proteins of rat liver peroxisomes. cDNA clones for PMP70 were isolated and sequenced. The predicted amino acid sequence (659 amino acid residues) revealed that the carboxyl-terminal region of PMP70 has strong sequence similarities to a group of ATP-binding proteins such as MalK and Mdr. These proteins form a superfamily and are involved in various biological processes including membrane transport. Limited protease treatment of peroxisomes showed that the ATP-binding domain of PMP70 is exposed to the cytosol. The hydropathy profile, in comparison with those of several other members of the ATP-binding protein superfamily, suggests that PMP70 is a transmembrane protein possibly forming a channel. Based on these results, we propose that PMP70 is involved in active transport across the peroxisomal membrane.

• Publication year

  1990

• Venue

  Journal of Biological Chemistry

• Publication date

  1990-03-15

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/s0021-9258(19)39595-xPMID 1968461
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Light and electron microscopic localization of l-alpha-hydroxyacid oxidase in rat kidney revealed by immunocytochemical techniques

  2004cited by this paper
• Peroxisome targeting signal of rat liver acyl-coenzyme A oxidase resides at the carboxy terminus

  1989cited by this paper
• A conserved tripeptide sorts proteins to peroxisomes

  1989cited by this paper
• Peroxisomal membrane ghosts in Zellweger syndrome--aberrant organelle assembly.

  1988cited by this paper
• Detection of an ATPase activity in rat liver peroxisomes.

  1988cited by this paper
• A family of closely related ATP‐binding subunits from prokaryotic and eukaryotic cells

  1988cited by this paper
• The ndvA gene product of Rhizobium meliloti is required for beta-(1----2)glucan production and has homology to the ATP-binding export protein HlyB

  1988cited by this paper
• Identification of peroxisomal targeting signals located at the carboxy terminus of four peroxisomal proteins

  1988cited by this paper
• Secretion of cyclolysin, the calmodulin‐sensitive adenylate cyclase‐haemolysin bifunctional protein of Bordetella pertussis.

  1988cited by this paper
• Purification of the 170- to 180-kilodalton membrane glycoprotein associated with multidrug resistance. 170- to 180-kilodalton membrane glycoprotein is an ATPase.

  1988cited by this paper
• ras genes.

  1987cited by this paper
• Biosynthesis of membrane polypeptides of rat liver peroxisomes.

  1987cited by this paper
• Integral membrane polypeptides of rat liver peroxisomes: topology and response to different metabolic states.

  1987cited by this paper
• A proton-translocating adenosine triphosphatase is associated with the peroxisomal membrane of yeasts

  1987cited by this paper
• Identification of a peroxisomal targeting signal at the carboxy terminus of firefly luciferase

  1987cited by this paper
• Translocation of acyl-CoA oxidase into peroxisomes requires ATP hydrolysis but not a membrane potential

  1987cited by this paper
• Molecular characterization of the oligopeptide permease of Salmonella typhimurium.

  1987cited by this paper
• An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs.

  1987cited by this paper
• A small-scale five-hour procedure for isolating multiple samples of CsCl-purified DNA: application to isolations from mammalian, insect, higher plant, algal, yeast, and bacterial sources.

  1986cited by this paper
• A family of related ATP-binding subunits coupled to many distinct biological processes in bacteria

  1986cited by this paper
• Homology between P-glycoprotein and a bacterial haemolysin transport protein suggests a model for multidrug resistance

  1986cited by this paper
• Domainal evolution of a prokaryotic DNA repair protein and its relationship to active-transport proteins

  1986cited by this paper
• Bacterial periplasmic transport systems: structure, mechanism, and evolution.

  1986cited by this paper
• Internal duplication and homology with bacterial transport proteins in the mdr1 (P-glycoprotein) gene from multidrug-resistant human cells.

  1986cited by this paper
• Purification of membrane polypeptides of rat liver peroxisomes.

  1986cited by this paper
• The nodI gene product of Rhizobium leguminosarum is closely related to ATP-binding bacterial transport proteins; nucleotide sequence analysis of the nodI and nodJ genes.

  1986cited by this paper
• Peroxisomal disorders: A newly recognised group of genetic diseases

  1986cited by this paper
• Mammalian multidrug resistance gene: complete cDNA sequence indicates strong homology to bacterial transport proteins.

  1986cited by this paper
• A new cell division operon inEscherichia coli

  1986cited by this paper
• Partial disassembly of peroxisomes

  1985cited by this paper
• Biogenesis of peroxisomes.

  1985cited by this paper
• Rapid and sensitive protein similarity searches.

  1985cited by this paper
• DNA cloning : a practical approach

  1985cited by this paper
• Improved isolation and purification of rat liver peroxisomes by combined rate zonal and equilibrium density centrifugation.

  1985cited by this paper
• A simple and rapid procedure for high-yield isolation of essentially undegraded free and membrane-bound polysomes from rat liver.

  1985cited by this paper
• Nucleotide binding by membrane components of bacterial periplasmic binding protein‐dependent transport systems.

  1985cited by this paper
• Nucleotide sequence of an Escherichia coli chromosomal hemolysin

  1985cited by this paper
• Identification of human glucocorticoid receptor complementary DNA clones by epitope selection.

  1985cited by this paper
• Phosphate-specific transport system of Escherichia coli: nucleotide sequence and gene-polypeptide relationships

  1985cited by this paper
• ATP-binding sites in the membrane components of histidine permease, a periplasmic transport system.

  1984cited by this paper
• Expression of functional acetylcholine receptor from cloned cDNAs

  1984cited by this paper
• DNA sequence of the white locus of Drosophila melanogaster.

  1984cited by this paper
• Synthesis of a major integral membrane polypeptide of rat liver peroxisomes on free polysomes.

  1984cited by this paper
• A simple and very efficient method for generating cDNA libraries.

  1983cited by this paper
• The amino acid sequence of the beta-subunit of ATP synthase from bovine heart mitochondria.

  1983cited by this paper
• Complete nucleotide sequence and identification of membrane components of the histidine transport operon of S. typhimurium

  1982cited by this paper
• Sequence of the malK gene in E.coli K12.

  1982cited by this paper
• Polypeptide and phospholipid composition of the membrane of rat liver peroxisomes: comparison with endoplasmic reticulum and mitochondrial membranes

  1982cited by this paper
• A simple method for displaying the hydropathic character of a protein.

  1982cited by this paper
• Purification and properties of the uvrA protein from Escherichia coli.

  1982cited by this paper
• Distantly related sequences in the alpha‐ and beta‐subunits of ATP synthase, myosin, kinases and other ATP‐requiring enzymes and a common nucleotide binding fold.

  1982cited by this paper
• The bacteriorhodopsin gene.

  1981cited by this paper
• Factors influencing palmitoyl-CoA oxidation by rat liver peroxisomal fractions. Substrate concentration, organelle integrity and ATP.

  1980cited by this paper
• Sequence of the lactose permease gene

  1980cited by this paper
• DNA sequencing with chain-terminating inhibitors.

  1977cited by this paper
• The amino-acid sequence of sarcine adenylate kinase from skeletal muscle.

  1974cited by this paper
• Peroxisomal and Mitochondrial Defects in the Cerebro-Hepato-Renal Syndrome

  1973cited by this paper
• Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.

  1972cited by this paper

• Metabolic phenotypes and fatty acid profiles associated with histopathology of primary aldosteronism

  2025cites this paper
• ATM deficiency differentially affects expression of proteins related to fatty acid oxidation and oxidative stress in a sex-specific manner in response to Western-type diet prior to and following myocardial infarction.

  2024cites this paper
• Pathophysiology of X-Linked Adrenoleukodystrophy: Updates on Molecular Mechanisms

  2024cites this paper
• Fluorescent fatty acid conjugates for live cell imaging of peroxisomes

  2024influential citation
• Structural insights into human ABCD3-mediated peroxisomal acyl-CoA translocation

  2024cites this paper
• Abcd1 deficiency accelerates cuprizone-induced oligodendrocyte loss and axonopathy in a demyelinating mouse model of X-linked adrenoleukodystrophy

  2023cites this paper
• Structural and functional insights of the human peroxisomal ABC transporter ALDP

  2022cites this paper
• Structural basis of substrate recognition and translocation by human very long-chain fatty acid transporter ABCD1

  2022cites this paper
• The Cys-N-degron pathway modulates pexophagy through the N-terminal oxidation and arginylation of ACAD10

  2022cites this paper
• Plastic structures for diverse substrates: A revisit of human ABC transporters

  2022cites this paper
• The lysosomal protein ABCD4 can transport vitamin B12 across liposomal membranes in vitro

  2021cites this paper
• The peroxisomal transporter ABCD3 plays a major role in dicarboxylic fatty acid metabolism

  2021cites this paper
• Acyl-CoA thioesterase activity of peroxisomal ABC protein ABCD1 is required for the transport of very long-chain acyl-CoA into peroxisomes

  2021cites this paper
• Structure insights of the human peroxisomal ABC transporter ALDP

  2021cites this paper
• Structures of the human peroxisomal fatty acid transporter ABCD1 in a lipid environment

  2021cites this paper
• The peroxisomal transporter ABCD3 plays a major role in hepatic dicarboxylic fatty acid metabolism and lipid homeostasis

  2021cites this paper
• Structural basis of substrate recognition and translocation by human ABCD1

  2021cites this paper
• Peroxisomal ABC Transporters: An Update

  2021cites this paper
• The transferred translocases: An old wine in a new bottle

  2021cites this paper
• Peroxisomal Cofactor Transport

  2020cites this paper
• Evolution of adrenoleukodystrophy model systems

  2020cites this paper
• Lysosomal inhibition attenuates peroxisomal gene transcription via suppression of PPARA and PPARGC1A levels

  2019cites this paper
• Biogenesis and Function of Peroxisomes in Human Disease with a Focus on the ABC Transporter.

  2019cites this paper
• The Function of the Peroxisome

  2019cites this paper
• Structural snapshot of the cholesterol-transport ATP-binding cassette proteins 1.

  2019cites this paper
• Plant peroxisomal solute transporter proteins

  2019cites this paper
• Peroxisome Biogenesis

  2019cites this paper
• Unsymmetric dihydropyridines bearing 2-pyridyl methyl carboxylate as modulators of P-glycoprotein; synthesis and biological evaluation in resistant and non-resistant cancer cells

  2019cites this paper
• Using Pull Down Strategies to Analyze the Interactome of Peroxisomal Membrane Proteins in Human Cells.

  2018cites this paper
• Predictive Structure and Topology of Peroxisomal ATP-Binding Cassette (ABC) Transporters

  2017cites this paper
• Clinical or ATPase domain mutations in ABCD4 disrupt the interaction between the vitamin B12-trafficking proteins ABCD4 and LMBD1

  2017cites this paper
• Peroxisomal ATP-binding cassette transporters form mainly tetramers

  2017cites this paper
• The peroxisomal AAA ATPase complex prevents pexophagy and development of peroxisome biogenesis disorders

  2017cites this paper
• Determining the Topology of Peroxisomal Proteins Using Protease Protection Assays.

  2017cites this paper
• Nrf2 and rip140 as new therapeutic targets for x-linked adrenoleukodystrophy (x-ald): control of redox/metabolic homeostasis and inflammation

  2017influential citation
• UvA-DARE ( Digital Academic Repository ) Lipotoxicity in adrenoleukodystrophy Size matters !

  2017cites this paper
• Assembly of Peroxisomal Membrane Proteins via the Direct Pex19p‐Pex3p Pathway

  2016cites this paper
• Identification and Characterization of a Large Protein Essential for Degradation of the Crystalline Region of Cellulose by Cytophaga hutchinsonii

  2016cites this paper
• Translocation of the ABC transporter ABCD4 from the endoplasmic reticulum to lysosomes requires the escort protein LMBD1

  2016cites this paper
• Estudios de biomarcadores y de posibles terapias en la deficiencia del transportador de creatina y en la enfermedad de niemann-pick tipo c

  2016cites this paper
• ABC Transporter Subfamily D: Distinct Differences in Behavior between ABCD1–3 and ABCD4 in Subcellular Localization, Function, and Human Disease

  2016cites this paper
• Brain endothelial dysfunction in cerebral adrenoleukodystrophy.

  2015cites this paper
• Peroxisomal ABC transporters: functions and mechanism

  2015cites this paper
• ABCD1 deletion‐induced mitochondrial dysfunction is corrected by SAHA: implication for adrenoleukodystrophy

  2015cites this paper
• Structure-Function Analysis of Peroxisomal ATP-binding Cassette Transporters Using Chimeric Dimers*

  2014cites this paper
• Pathophysiology of X-linked adrenoleukodystrophy☆

  2014cites this paper
• LXR antagonists induce ABCD2 expression.

  2014cites this paper
• A role for the human peroxisomal half-transporter ABCD3 in the oxidation of dicarboxylic acids.

  2014cites this paper
• Proteomic analysis of the peroxisomal membrane of Penicillium chrysogenum

  2014cites this paper
• ABC transporters as therapeutic targets for liver fibrosis

  2014cites this paper
• Impaired Very Long-chain Acyl-CoA β-Oxidation in Human X-linked Adrenoleukodystrophy Fibroblasts Is a Direct Consequence of ABCD1 Transporter Dysfunction*

  2013cites this paper
• X-linked Adrenoleukodystrophy

  2013cites this paper
• Etude structure/fonction du demi-transporteur ABCD2 dans le contexte de l'Adrénoleucodystrophie liée à l'X

  2013cites this paper
• Peroxisomal ABC transporters: structure, function and role in disease.

  2012cites this paper
• UvA-DARE ( Digital Academic Repository ) Molecular biology and pharmacogenetics of x-linked adrenoleukodystrophy

  2012cites this paper
• UvA-DARE ( Digital Academic Repository ) Molecular biology and pharmacogenetics of x-linked adrenoleukodystrophy

  2012cites this paper
• Very Long Chain Fatty Acid β-Oxidation in Astrocytes : Contribution of the ABCD 1-Dependent and-Independent Pathways

  2012cites this paper
• A novel mutation in the ABCD1 gene of a Korean boy diagnosed with X-linked adrenoleukodystrophy.

  2012cites this paper
• Translational studies in X-linked adrenoleukodystrophy

  2012cites this paper
• Very long chain fatty acid β-oxidation in astrocytes: contribution of the ABCD1-dependent and -independent pathways.

  2012cites this paper
• Pex5p Imports Folded Tetrameric Catalase by Interaction with Pex13p

  2012cites this paper
• Substrate Specificity Overlap and Interaction between Adrenoleukodystrophy Protein (ALDP/ABCD1) and Adrenoleukodystrophy-related Protein (ALDRP/ABCD2)*

  2011cites this paper
• ATP binding cassette transporter G1 (ABCG1) is an intracellular sterol transporter

  2011cites this paper
• THE ABSENCE OF ABCD2 REVEALS A NOVEL ROLE FOR PEROXISOMES IN THE PROTECTION FROM METABOLIC SYNDROME

  2011cites this paper
• Differential substrate specificities of human ABCD1 and ABCD2 in peroxisomal fatty acid β-oxidation.

  2011cites this paper
• Identification of a New Fatty Acid Synthesis-Transport Machinery at the Peroxisomal Membrane*

  2011cites this paper
• Glycosomal ABC transporters of Trypanosoma brucei: characterisation of their expression, topology and substrate specificity.

  2011cites this paper
• Mammalian peroxisomal ABC transporters: from endogenous substrates to pathology and clinical significance

  2011influential citation
• ABC subfamily D proteins and very long chain fatty acid metabolism as novel targets in adrenoleukodystrophy.

  2011cites this paper
• The Arabidopsis Peroxisomal ABC Transporter, Comatose, Complements the Saccharomyces cerevisiae pxa1 pxa2Δ Mutant for Metabolism of Long-chain Fatty Acids and Exhibits Fatty Acyl-CoA-stimulated ATPase Activity*

  2010cites this paper
• Characterizing the Interactomes of ABC Transporters PXA1 and PXA2 using the Integrated Split-Ubiquitin Membrane Yeast Two-hybrid System

  2010cites this paper
• Identification of a Substrate-binding Site in a Peroxisomal β-Oxidation Enzyme by Photoaffinity Labeling with a Novel Palmitoyl Derivative*

  2010cites this paper
• Visualization of subcellular NAD pools and intra-organellar protein localization by poly-ADP-ribose formation

  2010cites this paper
• Untersuchungen zum Fettsäuretransport durch zelluläre und peroxisomale Membranen

  2010cites this paper
• Peroxisomal Dysfunction in Inflammatory Childhood White Matter Disorders: An Unexpected Contributor to Neuropathology

  2009cites this paper
• Gemfibrozil, stretching arms beyond lipid lowering

  2009cites this paper
• Peroxisomes, Myelination, and Axonal Integrity in the CNS

  2009cites this paper
• 70-kDa peroxisomal membrane protein related protein (P70R/ABCD4) localizes to endoplasmic reticulum not peroxisomes, and NH2-terminal hydrophobic property determines the subcellular localization of ABC subfamily D proteins.

  2009cites this paper
• Peroxisomal proteomics: biomonitoring in mussels after the Prestige's oil spill.

  2009cites this paper
• Adrenoleukodystrophy: Molecular, Metabolic, Pathologic, and Therapeutic Aspects

  2009cites this paper
• Induction of the adrenoleukodystrophy-related gene (ABCD2) by thyromimetics.

  2009cites this paper
• A key role for the peroxisomal ABCD2 transporter in fatty acid homeostasis.

  2009cites this paper
• The human peroxisomal ABC half transporter ALDP functions as a homodimer and accepts acyl–CoA esters

  2008cites this paper
• Effect of dietary polyunsaturated fatty acids on the expression of peroxisomal ABC transporters.

  2008cites this paper
• Cholesterol-deprivation increases mono-unsaturated very long-chain fatty acids in skin fibroblasts from patients with X-linked adrenoleukodystrophy.

  2008cites this paper
• 48-ALD TRANSDUCTION OF ALD CD 34 + CELLS 1027

  2008cites this paper
• UvA-DARE ( Digital Academic Repository ) Metabolite transport across the peroxisomal membrane

  2008cites this paper
• Mutational analysis of X-linked adrenoleukodystrophy gene

  2007cites this paper
• Adrenoleukodystrophy--a new mutation identified.

  2007cites this paper
• Gene expression profiling in the lung and liver of PFOA-exposed mouse fetuses.

  2007cites this paper
• The 70-kDa peroxisomal membrane protein (PMP70) an ATP-binding cassette transporter

  2007cites this paper
• Hydrophobic Regions Adjacent to Transmembrane Domains 1 and 5 Are Important for the Targeting of the 70-kDa Peroxisomal Membrane Protein*

  2007cites this paper
• The peroxisomal ABC transporter family

  2007cites this paper
• Peroxisomal lipid degradation via β-and α-oxidation in Mammals

  2007cites this paper
• Peroxisome biogenesis and molecular defects in peroxisome assembly disorders

  2007cites this paper
• X-linked adrenoleukodystrophy: very long-chain fatty acid metabolism, ABC half-transporters and the complicated route to treatment.

  2007cites this paper
• Very-long-chain fatty acid metabolism in adrenoleukodystrophy protein-deficient mice

  2007cites this paper
• Live Cell FRET Microscopy

  2007cites this paper
• Dehydroepiandrosterone up-regulates the Adrenoleukodystrophy-related gene (ABCD2) independently of PPARalpha in rodents.

  2007cites this paper
• Acyl-CoA thioesterases - auxiliary enzymes in peroxisomal lipid metabolism

  2007cites this paper