Gaa1p and gpi8p are components of a glycosylphosphatidylinositol (GPI) transamidase that mediates attachment of GPI to proteins.

No abstract is available for this paper.

• Publication year

  2000

• Venue

  Molecular Biology of the Cell

• Publication date

  2000-05-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1091/MBC.11.5.1523PMID 10793132PMCID PMC14864
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Phosphatidylinositol Glycan ( PIG ) Anchored Membrane Proteins

  2001cited by this paper
• Asparaginyl Endopeptidase of Jack Bean Seeds

  2001cited by this paper
• Developmental abnormalities of glycosylphosphatidylinositol-anchor-deficient embryos revealed by Cre/loxP system.

  1999cited by this paper
• A Cell-free Assay for Glycosylphosphatidylinositol Anchoring in African Trypanosomes

  1999cited by this paper
• Human and mouse GPAA1 (Glycosylphosphatidylinositol anchor attachment 1) genes: genomic structures, chromosome loci and the presence of a minor class intron

  1999cited by this paper
• DPM2 regulates biosynthesis of dolichol phosphate‐mannose in mammalian cells: correct subcellular localization and stabilization of DPM1, and binding of dolichol phosphate

  1998cited by this paper
• Molecular cloning of human homolog of yeast GAA1 which is required for attachment of glycosylphosphatidylinositols to proteins 1

  1998cited by this paper
• The hydrophobic domains in the carboxyl-terminal signal for GPI modification and in the amino-terminal leader peptide have similar structural requirements.

  1998cited by this paper
• The first step of glycosylphosphatidylinositol biosynthesis is mediated by a complex of PIG‐A, PIG‐H, PIG‐C and GPI1

  1998cited by this paper
• The affected gene underlying the class K glycosylphosphatidylinositol (GPI) surface protein defect codes for the GPI transamidase.

  1997influential reference
• Mutational analysis of the C-terminal signal peptide of bovine liver 5'-nucleotidase for GPI anchoring: a study on the significance of the hydrophilic spacer region.

  1997cited by this paper
• In silicio identification of glycosyl‐phosphatidylinositol‐anchored plasma‐membrane and cell wall proteins of Saccharomyces cerevisiae

  1997cited by this paper
• Tissue-specific knockout of the mouse Pig-a gene reveals important roles for GPI-anchored proteins in skin development.

  1997cited by this paper
• Architecture of the Yeast Cell Wall

  1997cited by this paper
• GPI-anchor deficient mice : implications for clonal dominance of mutant cells in paroxysmal nocturnal hemoglobinuria

  1996cited by this paper
• A defect in glycosylphosphatidylinositol (GPI) transamidase activity in mutant K cells is responsible for their inability to display GPI surface proteins.

  1996influential reference
• Cloning and characterization of the murine GPI anchor synthesis gene Pigf, a homologue of the human PIGF gene.

  1996cited by this paper
• Glycosylphosphatidylinositol-anchor-deficient mice: implications for clonal dominance of mutant cells in paroxysmal nocturnal hemoglobinuria.

  1996cited by this paper
• Yeast Gpi8p is essential for GPI anchor attachment onto proteins.

  1996influential reference
• Stable production of mutant mice from double gene converted ES cells with puromycin and neomycin.

  1995cited by this paper
• Glycosyl phosphatidylinositol-dependent cross-linking of alpha- agglutinin and beta 1,6-glucan in the Saccharomyces cerevisiae cell wall

  1995cited by this paper
• How glycosylphosphatidylinositol-anchored membrane proteins are made.

  1995influential reference
• An Active Carbonyl Formed during Glycosylphosphatidylinositol Addition to a Protein Is Evidence of Catalysis by a Transamidase (*)

  1995cited by this paper
• Cleavage without anchor addition accompanies the processing of a nascent protein to its glycosylphosphatidylinositol-anchored form.

  1995influential reference
• GPI-anchor biosynthesis.

  1995cited by this paper
• Temperature-sensitive Yeast GPI Anchoring Mutants gpi2 and gpi3 Are Defective in the Synthesis of N-Acetylglucosaminyl Phosphatidylinositol.

  1995cited by this paper
• A Putative Vacuolar Processing Protease Is Regulated by Ethylene and also during Fruit Ripening in Citrus Fruit

  1995influential reference
• Yeast Gaa1p is required for attachment of a completed GPI anchor onto proteins

  1995cited by this paper
• Defective glycosyl phosphatidylinositol anchor synthesis and paroxysmal nocturnal hemoglobinuria.

  1995cited by this paper
• Blasticidin S deaminase gene from Aspergillus terreus (BSD): a new drug resistance gene for transfection of mammalian cells.

  1994cited by this paper
• 2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans

  1994cited by this paper
• Microsomal aldehyde dehydrogenase is localized to the endoplasmic reticulum via its carboxyl-terminal 35 amino acids

  1994influential reference
• Glycoinositol phospholipid anchor-defective K562 mutants with biochemical lesions distinct from those in Thy-1- murine lymphoma mutants.

  1994cited by this paper
• Asparaginyl endopeptidase of jack bean seeds. Purification, characterization, and high utility in protein sequence analysis.

  1993cited by this paper
• Deficiency of the GPI anchor caused by a somatic mutation of the PIG-A gene in paroxysmal nocturnal hemoglobinuria.

  1993cited by this paper
• Analysis of the sequence requirements for glycosylphosphatidylinositol anchoring of Saccharomyces cerevisiae Gas1 protein.

  1993cited by this paper
• Phosphatidylinositol glycan (PI-G) anchored membrane proteins. Amino acid requirements adjacent to the site of cleavage and PI-G attachment in the COOH-terminal signal peptide.

  1992cited by this paper
• Characterization of putative glycoinositol phospholipid anchor precursors in mammalian cells. Localization of phosphoethanolamine.

  1992influential reference
• Determinants for glycophospholipid anchoring of the Saccharomyces cerevisiae GAS1 protein to the plasma membrane

  1991cited by this paper
• Glycophospholipid membrane anchor attachment. Molecular analysis of the cleavage/attachment site.

  1991cited by this paper
• Biosynthesis of phosphatidylinositol glycan-anchored membrane proteins. Design of a simple protein substrate to characterize the enzyme that cleaves the COOH-terminal signal peptide.

  1991influential reference
• Neonatal lethality and lymphopenia in mice with a homozygous disruption of the c-abl proto-oncogene.

  1991cited by this paper
• Selectivity of the cleavage/attachment site of phosphatidylinositol-glycan-anchored membrane proteins determined by site-specific mutagenesis at Asp-484 of placental alkaline phosphatase.

  1990cited by this paper
• The glycosyl-phosphatidylinositol anchor of membrane proteins.

  1989influential reference
• Conversion of a PI-anchored protein to an integral membrane protein by a single amino acid mutation.

  1988cited by this paper
• Molecular cloning and sequence analysis of human placental alkaline phosphatase.

  1986cited by this paper

• Integrative analysis identifies PIGK as an oncogenic glycosylphosphatidylinositol transamidase subunit with prognostic, immunological, and therapeutic relevance in head and neck cancer

  2026cites this paper
• Structure and Function of the Glycosylphosphatidylinositol Transamidase, a Transmembrane Complex Catalyzing GPI Anchoring of Proteins.

  2024cites this paper
• Proteomic screens of SEL1L-HRD1 ER-associated degradation substrates reveal its role in glycosylphosphatidylinositol-anchored protein biogenesis

  2024cites this paper
• Towards a thorough understanding of mammalian glycosylphosphatidylinositol-anchored protein biosynthesis.

  2024cites this paper
• Glycosylation editing: an innovative therapeutic opportunity in precision oncology

  2024cites this paper
• Substrate-binding Glycine Residues are Major Determinants for Hydrolase and Ligase Activity of Plant Legumains.

  2023cites this paper
• Substrate-binding Glycine Residues are Major Determinants for Hydrolase and Ligase Activity of Plant Legumains

  2022cites this paper
• A Soluble, Minimalistic Glycosylphosphatidylinositol Transamidase (GPI-T) Retains Transamidation Activity.

  2022cites this paper
• Molecular insights into biogenesis of glycosylphosphatidylinositol anchor proteins

  2022cites this paper
• Structure of human glycosylphosphatidylinositol transamidase

  2022cites this paper
• Live imaging of retinotectal mapping reveals topographic map dynamics and a novel role for Contactin-2 in map sharpening.

  2021cites this paper
• Loss of PIGK function causes severe infantile encephalopathy and extensive neuronal apoptosis

  2021cites this paper
• Potential Physiological Relevance of ERAD to the Biosynthesis of GPI-Anchored Proteins in Yeast

  2021cites this paper
• Subunits of the GPI transamidase complex localize to the endoplasmic reticulum and nuclear envelope in Drosophila

  2021cites this paper
• Computational prediction of the effects of non-synonymous single nucleotide polymorphisms on the GPI-anchor transamidase subunit GPI8p of Plasmodium falciparum

  2021cites this paper
• Glycans of the Pathogenic Yeast Cryptococcus neoformans and Related Opportunities for Therapeutic Advances

  2021cites this paper
• Hrd1-dependent Degradation of the Unassembled PIGK Subunit of the GPI Transamidase Complex

  2021cites this paper
• Functional Analysis of the GPI Transamidase Complex by Screening for Amino Acid Mutations in Each Subunit

  2021cites this paper
• CELL STRUCTURE AND FUNCTION Advance Publication by J-STAGE

  2021cites this paper
• The Glycosylphosphatidylinositol Anchor: A Linchpin for Cell Surface Versatility of Trypanosomatids

  2021cites this paper
• The consensus Nglyco-X-S/T motif and a previously unknown Nglyco-N -linked glycosylation are necessary for growth and pathogenicity of Phytophthora

  2020cites this paper
• Activity-dependent refinement of nasal retinal projections drives topographic map sharpening in the teleost visual system

  2020influential citation
• Glycosylphosphatidylinositol Anchors and Lipids

  2020cites this paper
• Selecting cells expressing high levels of recombinant proteins using the GPI-anchored protein with selenocysteine system.

  2020cites this paper
• The caspase-like Gpi8 subunit of Candida albicans GPI transamidase is a metal-dependent endopeptidase.

  2020cites this paper
• The mammalian cytosolic thioredoxin reductase pathway acts via a membrane protein to reduce ER-localised proteins

  2019cites this paper
• Generating anchors only to lose them: The unusual story of glycosylphosphatidylinositol anchor biosynthesis and remodeling in yeast and fungi

  2018cites this paper
• Investigation Of The Saccharomyces Cerevisiae Gpi Transamidase: Insights Into Its Activity And Subunit-Subunit Interactions

  2018influential citation
• Molecular switching system using glycosylphosphatidylinositol to select cells highly expressing recombinant proteins

  2017cites this paper
• Synthesis, Genetics, and Congenital Diseases of GPI-Anchored Proteins

  2017cites this paper
• Disulfide Bond Formation and N-Glycosylation Modulate Protein-Protein Interactions in GPI-Transamidase (GPIT)

  2017cites this paper
• Pathogenesis of Clonal Dominance in PNH: Growth Advantage in PNH

  2017cites this paper
• Mutations in GPAA1, Encoding a GPI Transamidase Complex Protein, Cause Developmental Delay, Epilepsy, Cerebellar Atrophy, and Osteopenia.

  2017cites this paper
• Biosynthesis of GPI-anchored proteins: special emphasis on GPI lipid remodeling

  2016cites this paper
• Structure and function of legumain in health and disease.

  2016cites this paper
• Biochemical And Structural Characterization Of The Core Subunits Of Gpi Transamidase

  2015cites this paper
• Structural changes of GPI anchor after its attachment to proteins: functional significance.

  2015cites this paper
• Nanoparticle formulation enhanced protective immunity provoked by PYGPI8p-transamidase related protein (PyTAM) DNA vaccine in Plasmodium yoelii malaria model.

  2014cites this paper
• Transamidase subunit GAA1/GPAA1 is a M28 family metallo-peptide-synthetase that catalyzes the peptide bond formation between the substrate protein’s omega-site and the GPI lipid anchor’s phosphoethanolamine

  2014cites this paper
• Biosynthesis and deficiencies of glycosylphosphatidylinositol

  2014cites this paper
• Transcriptome and Biochemical Analysis Reveals That Suppression of GPI-Anchor Synthesis Leads to Autophagy and Possible Necroptosis in Aspergillus fumigatus

  2013cites this paper
• Structural and biochemical characterisation of enzymes involved in mannan biosynthesis

  2013cites this paper
• Development And Optimization Of The First High Throughput In Vitro Fret Assay To Characterize The Saccharomyces Cerevisiae Gpi-T

  2013influential citation
• Characterization of a glycosyphosphatidylinositol anchor transamidase in Arabidopsis thaliana and the function of GPI anchored proteins in stomatal development

  2013influential citation
• Glycosylphosphatidylinositol: Protein Transamidase

  2013cites this paper
• GPI Transamidase and GPI anchored proteins: Oncogenes and biomarkers for cancer

  2013cites this paper
• Low-resolution structure of the soluble domain GPAA1 (yGPAA170–247) of the glycosylphosphatidylinositol transamidase subunit GPAA1 from Saccharomyces cerevisiae

  2013cites this paper
• Architecture and Biosynthesis of the Saccharomyces cerevisiae Cell Wall

  2012cites this paper
• Insights into the distribution and functions of the eukaryotic GPI-like anchored genes among Mycobacterium from a comparative genomic perspective.

  2012cites this paper
• Thy-1 Signaling in T cells is Weaker and Has Delayed Signaling Kinetics, Promotes Delayed Acquisition and Triggering of Cytotoxic Effector Function, and Preferentially Promotes IL-17A and IL-4 Production in Comparison to TcR Signaling

  2012cites this paper
• Defining the boundaries of species specificity for the Saccharomyces cerevisiae glycosylphosphatidylinositol transamidase using a quantitative in vivo assay

  2012cites this paper
• Proteomic Identification of Glycosylphosphatidylinositol Anchor-dependent Membrane Proteins Elevated in Breast Carcinoma*

  2012cites this paper
• A forward genetic screen to study mammalian RNA interference – essential role of RNase IIIa domain of Dicer1 in 3′ strand cleavage of dsRNA in vivo

  2012cites this paper
• Efficient Glycosylphosphatidylinositol (GPI) Modification of Membrane Proteins Requires a C-terminal Anchoring Signal of Marginal Hydrophobicity*

  2012cites this paper
• Protein translocation across the ER membrane.

  2011cites this paper
• Protein Glycosylation in Aspergillus fumigatus Is Essential for Cell Wall Synthesis and Serves as a Promising Model of Multicellular Eukaryotic Development

  2011cites this paper
• Structural remodeling, trafficking and functions of glycosylphosphatidylinositol-anchored proteins.

  2011cites this paper
• Structural insight into the glycosylphosphatidylinositol transamidase subunits PIG-K and PIG-S from yeast.

  2011cites this paper
• transamidase, Touch response Biogenesis of GPI-anchored proteins is essential for surface expression of sodium channels in zebrafish Rohon-Beard neurons to respond

  2010cites this paper
• Methods to Study GPI Anchoring of Proteins

  2010cites this paper
• Biogenesis of GPI-anchored proteins is essential for surface expression of sodium channels in zebrafish Rohon-Beard neurons to respond to mechanosensory stimulation

  2010cites this paper
• transamidase, Touch response Biogenesis of GPI-anchored proteins is essential for surface expression of sodium channels in zebrafish Rohon-Beard neurons to respond

  2010cites this paper
• The Biochemistry Department of the University of Geneva: Understanding the Molecular Basis and Function of Intracellular Organization.

  2009cites this paper
• Chapter 7 Attachment of a GPI Anchor to Protein

  2009cites this paper
• Trypanosome glycosylphosphatidylinositol biosynthesis.

  2009cites this paper
• Characterization of GPIT-1 and GPIT-2, two auxiliary components of the Neurospora crassa GPI transamidase complex

  2009cites this paper
• Chapter 1 Overview of GPI Biosynthesis

  2009cites this paper
• Proteinas de superficie de Paracoccidioides brasiliensis

  2008influential citation
• Exploring the fungal wall proteome by mass spectrometry

  2008cites this paper
• Profiling the expression pattern of GPI transamidase complex subunits in human cancer

  2008cites this paper
• Dynamic Regulation of p 53 Subnuclear Localization and Senescence by MORC 3 □

  2007cites this paper
• UvA-DARE ( Digital Academic Repository ) Exploring the fungal wall proteome by mass spectrometry

  2007cites this paper
• Mass spectrometric quantitation of covalently bound cell wall proteins in Saccharomyces cerevisiae

  2007cites this paper
• Glycosylphosphatidylinositol (GPI) anchor is required in Aspergillus fumigatus for morphogenesis and virulence

  2007cites this paper
• Dynamic regulation of p53 subnuclear localization and senescence by MORC3.

  2007cites this paper
• The Role of Cell‑Free Rabbit Reticulocyte Expression Systems in Functional Proteomics

  2007cites this paper
• Identification of a novel functional specificity signal within the GPI anchor signal sequence of carcinoembryonic antigen

  2007cites this paper
• Truncation of the caspase-related subunit (Gpi8p) of Saccharomyces cerevisiae GPI transamidase: dimerization revealed.

  2007cites this paper
• Biosynthesis and function of GPI proteins in the yeast Saccharomyces cerevisiae.

  2007cites this paper
• Alterations of GPI transamidase subunits in head and neck squamous carcinoma

  2007cites this paper
• Thematic review series: Lipid Posttranslational Modifications. GPI anchoring of protein in yeast and mammalian cells, or: how we learned to stop worrying and love glycophospholipids Published, JLR Papers in Press, March 14, 2007.

  2007influential citation
• Recent developments in the molecular, biochemical and functional characterization of GPI8 and the GPI-anchoring mechanism [Review]

  2006influential citation
• Ethanolamine Phosphate Linked to the First Mannose Residue of Glycosylphosphatidylinositol (GPI) Lipids Is a Major Feature of the GPI Structure That Is Recognized by Human GPI Transamidase*

  2006cites this paper
• TbGPI16 is an essential component of GPI transamidase in Trypanosoma brucei

  2006cites this paper
• Mutational Analysis of the Glycosylphosphatidylinositol (GPI) Anchor Pathway Demonstrates that GPI-Anchored Proteins Are Required for Cell Wall Biogenesis and Normal Hyphal Growth in Neurospora crassa

  2006cites this paper
• A new locus for autosomal dominant amelogenesis imperfecta on chromosome 8q24.3

  2006cites this paper
• CHO glycosylation mutants: GPI anchor.

  2006cites this paper
• New mutant Chinese hamster ovary cell representing an unknown gene for attachment of glycosylphosphatidylinositol to proteins.

  2005cites this paper
• Neurospora crassa Biogenesis and Normal Hyphal Growth in Proteins Are Required for Cell Wall Pathway Demonstrates that GPI-Anchored Glycosylphosphatidylinositol ( GPI ) Anchor Mutational Analysis of the

  2005cites this paper
• Screening for glycosylphosphatidylinositol-anchored proteins in the Paracoccidioides brasiliensis transcriptome.

  2005cites this paper
• Gpi17p does not stably interact with other subunits of glycosylphosphatidylinositol transamidase in Saccharomyces cerevisiae.

  2005cites this paper
• Protein posttranslational modifications: the chemistry of proteome diversifications.

  2005cites this paper
• GPI7 Is the Second Partner of PIG-F and Involved in Modification of Glycosylphosphatidylinositol*

  2005cites this paper
• Posttranslationale Proteinmodifikation: die Chemie der Proteomdiversifizierung

  2005cites this paper
• Deficiencies in the essential Smp3 mannosyltransferase block glycosylphosphatidylinositol assembly and lead to defects in growth and cell wall biogenesis in Candida albicans.

  2004influential citation
• Role of Herp in the endoplasmic reticulum stress response

  2004cites this paper
• A Conserved Proline in the Last Transmembrane Segment of Gaa1 Is Required for Glycosylphosphatidylinositol (GPI) Recognition by GPI Transamidase*

  2004cites this paper
• Proprotein interaction with the GPI transamidase

  2003influential citation
• Two Subunits of Glycosylphosphatidylinositol Transamidase, GPI8 and PIG-T, Form a Functionally Important Intermolecular Disulfide Bridge*

  2003cites this paper
• PIG-W is critical for inositol acylation but not for flipping of glycosylphosphatidylinositol-anchor.

  2003cites this paper