Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR

Background The ongoing outbreak of the recently emerged novel coronavirus (2019-nCoV) poses a challenge for public health laboratories as virus isolates are unavailable while there is growing evidence that the outbreak is more widespread than initially thought, and international spread through travellers does already occur. Aim We aimed to develop and deploy robust diagnostic methodology for use in public health laboratory settings without having virus material available. Methods Here we present a validated diagnostic workflow for 2019-nCoV, its design relying on close genetic relatedness of 2019-nCoV with SARS coronavirus, making use of synthetic nucleic acid technology. Results The workflow reliably detects 2019-nCoV, and further discriminates 2019-nCoV from SARS-CoV. Through coordination between academic and public laboratories, we confirmed assay exclusivity based on 297 original clinical specimens containing a full spectrum of human respiratory viruses. Control material is made available through European Virus Archive – Global (EVAg), a European Union infrastructure project. Conclusion The present study demonstrates the enormous response capacity achieved through coordination of academic and public laboratories in national and European research networks.

• Publication year

  2020

• Venue

  Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin

• Publication date

  2020-01-01

• Fields of study

  Medicine, Computer Science

• Identifiers
  DOI 10.2807/1560-7917.es.2020.25.3.2000045PMID 31992387PMCID 6988269
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• A novel 2019: Coronavirus

  2020influential reference
• Novel coronavirus (2019-nCoV)

  2020cited by this paper
• Advancing preparedness for clinical research during infectious disease epidemics

  2019cited by this paper
• Attenuation of replication by a 29 nucleotide deletion in SARS-coronavirus acquired during the early stages of human-to-human transmission

  2018cited by this paper
• Need for additional capacity and improved capability for molecular detection of yellow fever virus in European Expert Laboratories: External Quality Assessment, March 2018

  2018cited by this paper
• The European Virus Archive goes global: A growing resource for research

  2018cited by this paper
• Hosts and Sources of Endemic Human Coronaviruses

  2018cited by this paper
• Origin and evolution of pathogenic coronaviruses

  2018cited by this paper
• Developing a framework to assess the costeffectiveness of COMPARE - a global platform for the exchange of sequence-based pathogen data.

  2017cited by this paper
• Family Coronaviridae

  2017cited by this paper
• Assay optimization for molecular detection of Zika virus

  2016cited by this paper
• First international external quality assessment of molecular diagnostics for Mers-CoV

  2015cited by this paper
• Ecology, evolution and classification of bat coronaviruses in the aftermath of SARS

  2013cited by this paper
• Coronavirus

  2013cited by this paper
• Specific detection by real-time reverse-transcription PCR assays of a novel avian influenza A(H7N9) strain associated with human spillover infections in China.

  2013cited by this paper
• Assays for laboratory confirmation of novel human coronavirus (hCoV-EMC) infections.

  2012cited by this paper
• Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction.

  2012cited by this paper
• Genomic Characterization of Severe Acute Respiratory Syndrome-Related Coronavirus in European Bats and Classification of Coronaviruses Based on Partial RNA-Dependent RNA Polymerase Gene Sequences

  2010cited by this paper
• Coordinated Implementation of Chikungunya Virus Reverse Transcription–PCR

  2009cited by this paper
• First past the post

  2003cited by this paper
• The Severe Acute Respiratory Syndrome

  2003cited by this paper
• Identification of a novel coronavirus in patients with severe acute respiratory syndrome.

  2003cited by this paper
• Virus taxonomy: classification and nomenclature of viruses. Seventh report of the International Committee on Taxonomy of Viruses.

  2000cited by this paper
• THE WORLD HEALTH ORGANIZATION

  1954cited by this paper

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