Production of d-lactate from glucose using Klebsiella pneumoniae mutants

Backgroundd-Lactate is a valued chemical which can be produced by some bacteria including Klebsiella pneumoniae. However, only a few studies have focused on K. pneumoniae for d-lactate production with a significant amount of by-products, which complicated the purification process and decreased the yield of d-lactate.ResultsBased on the redirection of carbon towards by-product formation, the effects of single-gene and multiple-gene deletions in K. pneumoniae on d-lactate production from glucose via acetolactate synthase (budB), acetate kinase (ackA), and alcohol dehydrogenase (adhE) were tested. Klebsiella pneumoniae mutants had different production behaviours. The accumulation of the main by-products was decreased in the mutants. The triple mutant strain had the most powerful ability to produce optically pure d-lactate from glucose, and was tested with xylose and arabinose as carbon sources. Fed-batch fermentation was also carried out under various aeration rates, and the strain accumulated 125.1 g/L d-lactate with a yield of 0.91 g/g glucose at 2.5 vvm.ConclusionsKnocking out by-product synthesis genes had a remarkable influence on the production and yield of d-lactate. This study demonstrated, for the first time, that K. pneumoniae has great potential to convert monosaccharides into d-lactate. The results provide new insights for industrial production of d-lactate by K. pneumoniae.

• Publication year

  2017

• Venue

  Microbial Cell Factories

• Publication date

  2017-11-21

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1186/s12934-017-0822-6PMID 29162110PMCID 5697408
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• Fed-batch fermentation of the triple mutant at 2.5 vvm produced 125.1 g/L d-lactate with a yield of 0.91 g/g glucose.

  Confidence 0.98

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review
• The triple mutant strain showed the strongest ability to produce optically pure d-lactate from glucose.

  Confidence 0.97

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review
• Deleting budB, ackA, and adhE in Klebsiella pneumoniae reduced accumulation of the main by-products.

  Confidence 0.96

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review

• acka

  gene

  A gene encoding acetate kinase that was deleted as part of the metabolic engineering strategy.

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review
• adhe

  gene

  A gene encoding alcohol dehydrogenase that was deleted as part of the metabolic engineering strategy.

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review
• budb

  gene

  A gene encoding acetolactate synthase that was deleted to alter by-product formation.

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review
• d-lactate

  compound

  The D-enantiomer of lactate targeted as the desired fermentation product in this work.

  Aliases: d-lactic acid

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review
• fed-batch fermentation

  process

  A cultivation mode with stepwise substrate feeding used to evaluate lactate production under controlled aeration.

  Aliases: fed batch fermentation

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review
• klebsiella pneumoniae

  organism

  The bacterial host strain engineered and evaluated for lactate production.

  Aliases: K. pneumoniae

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review
• triple mutant strain

  strain

  A K. pneumoniae strain carrying deletions in budB, ackA, and adhE.

  Aliases: triple mutant

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review

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