The role of 5HT3 in nociceptive processing in the rat spinal cord: results from behavioural and electrophysiological studies.

Recent studies have suggested that activity at different 5HT receptor subtypes in the spinal cord either inhibits or facilitates nociceptive processing in the spinal cord. The present study has examined the role of 5HT3 receptors in nociceptive processing in the dorsal horn of the rat spinal cord. Using both behavioural and electrophysiological studies, 5HT3 ligands have been applied by a common route (i.e. intrathecal microinjection). In addition, only noxious heat has been used as a form of stimulation. Intrathecal injection of mCPBG (0.02-0.2 nmol) increased the responsiveness of dorsal horn neurones to noxious stimulation. In contrast, two 5HT3 antagonists (ICS 205-930 (0.015-0.15 nmol) and GR 380032F (ondansetron 34 nmol)) reduced nociceptive responses. Using doses which influenced nociceptive neuronal responses no significant change in TFL was recorded. These findings suggest that activation at 5HT3 receptor facilitates nociceptive responses of some dorsal horn neurones; these results are discussed in relation to earlier reports concerning alternative effects.

• Publication year

  1996

• Venue

  Neuroscience Letters

• Publication date

  1996-04-26

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/0304-3940(95)12600-7PMID 8733305
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Serotonin and pain: evidence that activation of 5‐HT1A receptors does not elicit antinociception against noxious thermal, mechanical and chemical stimuli in mice

  1994cited by this paper
• The actions of 5-HT1 agonists and antagonists on nociceptive processing in the rat spinal cord: results from behavioural and electrophysiological studies.

  1994cited by this paper
• 5-HT3 receptors in the rat central nervous system are mainly located on nerve fibres and terminals.

  1993cited by this paper
• 5-Hydroxytryptamine receptors.

  1992cited by this paper
• Attenuation of defensive analgesia in male mice by 5-HT3 receptor antagonists, ICS 205-930, MDL 72222, MDL 73147EF and MDL 72699.

  1992cited by this paper
• Analgesic effects of S and R isomers of the novel 5-HT3 receptor antagonists ADR-851 and ADR-882 in rats.

  1991cited by this paper
• Spinal 5-HT3 receptor-mediated antinociception: possible release of GABA

  1991cited by this paper
• Analgesic profile of centrally administered 2-methylserotonin against acute pain in rats.

  1991cited by this paper
• Evidence that spinal 5-HT1, 5-HT2 and 5-HT3 receptor subtypes modulate responses to noxious colorectal distension in the rat.

  1991cited by this paper
• Analgesic effects of serotonin and receptor-selective serotonin agonists in the rat spinal cord.

  1991cited by this paper
• 5-hydroxytryptamine (5-HT)1A receptors and the tail-flick response. I. 8-hydroxy-2-(di-n-propylamino) tetralin HBr-induced spontaneous tail-flicks in the rat as an in vivo model of 5-HT1A receptor-mediated activity.

  1990cited by this paper
• 1-(m-chlorophenyl)-biguanide, a potent high affinity 5-HT3 receptor agonist.

  1990cited by this paper
• The concept of selectivity in 5-HT receptor research.

  1990cited by this paper
• 5-HT3 receptors modulate spinal nociceptive reflexes.

  1990cited by this paper
• Distribution of Serotonin Receptors

  1990cited by this paper
• Influence of a specific 5‐HT3 antagonist on carrageenan‐induced hyperalgesia in rats

  1989cited by this paper
• 5-HT3 receptors are membrane ion channels

  1989cited by this paper
• Reversal of the antinociceptive effects of intrathecally administered serotonin in the rat by a selective 5-HT3 receptor antagonist.

  1988cited by this paper
• Serotonin innervation of physiologically identified lamina I projection neurons.

  1986cited by this paper
• Identification of serotonin M-receptor subtypes and their specific blockade by a new class of drugs

  1985cited by this paper
• Descending serotonergic, peptidergic and cholinergic pathways from the raphe nuclei: a multiple transmitter complex.

  1983cited by this paper
• Immunocytochemical analysis of serotonergic axons in laminae I and II of the lumbar spinal cord of the cat

  1982cited by this paper
• The topographical distribution of serotoninergic terminals in the spinal cord of the cat: biochemical mapping by the combined use of microdissection and microassay procedures.

  1977cited by this paper
• EVIDENCE FOR THE EXISTENCE OF MONOAMINE-CONTAINING NEURONS IN THE CENTRAL NERVOUS SYSTEM. I. DEMONSTRATION OF MONOAMINES IN THE CELL BODIES OF BRAIN STEM NEURONS.

  1964cited by this paper
• DYNAMIC RESPONSE OF CHEST WALL AND LUNG INJURIES IN RABBITS EXPOSED TO AIR SHOCK WAVES OF SHORT DURATION.

  1964cited by this paper

• Diffuse Noxious Inhibitory Controls in Chronic Pain States: Insights from Pre-Clinical Studies

  2025cites this paper
• A Comparative Study of Lignocaine with Ondansetron as Pretreatment to Prevent Pain on Injection of Propofol

  2022cites this paper
• Tropisetron attenuates neuroinflammation and chronic neuropathic pain via α7nAChR activation in the spinal cord in rats

  2022cites this paper
• Comparing the Effectiveness of Metoclopramide, Low Dose of Propofol, Ondansetron, and Magnesium Sulfate on Propofol Injection Pain: A Double-Blind Clinical Trial

  2022cites this paper
• The development of descending serotonergic modulation of the spinal nociceptive network: a life span perspective

  2021cites this paper
• Computational Approach Reveals Pronociceptive Potential of Cannabidiol in Osteoarthritis: Role of Transient Receptor Potential Channels

  2021cites this paper
• Serotonin—pain modulation

  2020cites this paper
• Rostral ventromedial medulla‐mediated descending facilitation following P2X7 receptor activation is involved in the development of chronic post‐operative pain

  2019influential citation
• Ondansetron attenuates bone cancer pain through the inhibition of Fos in the descending facilitation related nuclei

  2018cites this paper
• Priming with different doses of metoclopramide preceded by tourniquet alleviates propofol induced pain: A comparative study with lidocaine

  2018cites this paper
• Schmerzentstehung, Bildgebung und Schmerzmessung

  2018cites this paper
• Comparing palonosetron and lignocaine pretreatment with venous occlusion on reducing rocuronium associated withdrawal movements. A double blinded randomized control trial

  2018cites this paper
• The plasticity of descending controls in pain: translational probing

  2017cites this paper
• Le yin et le yang de la sérotonine : un Janus dans les contrôles de la douleur

  2017cites this paper
• An investigation into the inhibitory function of serotonin in diffuse noxious inhibitory controls in the neuropathic rat

  2017cites this paper
• Review: 5-HT1, 5-HT2, 5-HT3 and 5-HT7 Receptors and their Role in the Modulation of Pain Response in the Central Nervous System

  2017cites this paper
• An investigation into the inhibitory function of serotonin in diffuse noxious inhibitory controls in the neuropathic rat The inhibitory action of spinal serotonin in neuropathy

  2017cites this paper
• Instructions for use Title Endogenously released 5-HT inhibits A and C fiber-evoked synaptic transmission in the rat spinal cord by the facilitation of GABA / glycine and 5-HT release via 5-HT 2 A and 5-HT 3 receptors

  2016cites this paper
• What the brain tells the spinal cord.

  2016cites this paper
• Instructions for use Title Endogenously released 5-HT inhibits A and C fiber-evoked synaptic transmission in the rat spinal cord by the facilitation of GABA/glycine and 5-HT release via 5-HT2A and 5-HT3 receptors

  2016cites this paper
• Instructions for use Title Endogenously released 5-HT inhibits A and C fiber-evoked synaptic transmission in the rat spinal cord by the facilitation of GABA / glycine and 5-HT release via 5-HT 2 A and 5-HT 3 receptors

  2016cites this paper
• The electroacupuncture-induced analgesic effect mediated by 5-HT1, 5-HT3 receptor and muscarinic cholinergic receptors in rat model of collagenase-induced osteoarthritis

  2016cites this paper
• ONDANSETRON VERSUS LIDOCAINE 2% FOR THE PREVENTION OF PROPOFOL INJECTION INDUCED PAIN

  2016cites this paper
• Spinal 5-HT3 receptor mediates nociceptive effect on central neuropathic pain; possible therapeutic role for tropisetron

  2016cites this paper
• Clinical Spectrum of Flank Pain and ITS Association with Urolithiasis

  2015cites this paper
• Galanin-Mediated Behavioural Hyperalgesia from the Dorsomedial Nucleus of the Hypothalamus Involves Two Independent Descending Pronociceptive Pathways

  2015cites this paper
• Antinociceptive effects of AS1069562, the (+)-isomer of indeloxazine, on spinal hypersensitivity induced by intrathecal injection of prostaglandin in mice: comparison with duloxetine and amitriptyline.

  2014cites this paper
• Analgesic effects of palonosetron in the intravenous propofol injection

  2014cites this paper
• Effects of pretreatment with intravenous palonosetron for propofol-remifentanil-based anesthesia in breast and thyroid cancer surgery: a double-blind, randomized, controlled study

  2014cites this paper
• An altered spinal serotonergic system contributes to increased thermal nociception in an animal model of depression

  2014cites this paper
• Pronociception in experimental monoarthritis: the role of galanin in descending pathways

  2014cites this paper
• Spinal cord plasticity in peripheral inflammatory pain

  2014cites this paper
• Ondansetron versus Amitriptyline in the Treatment of Peripheral Neuropathy: A Randomized Double Blind Prospective Clinical Study

  2014cites this paper
• Tropisetron alleviate early post‐operative pain after gynecological laparoscopy in sevoflurane based general anaesthesia: A randomized, parallel‐group, factorial study

  2014influential citation
• Endogenously released 5-HT inhibits A and C fiber-evoked synaptic transmission in the rat spinal cord by the facilitation of GABA/glycine and 5-HT release via 5-HT(2A) and 5-HT(3) receptors.

  2013cites this paper
• DESCENDING CONTROL IN SENSITIZATION OF REFLEXES IN THE RAT

  2013cites this paper
• Peripheral and spinal 5-HT receptors participate in the pronociceptive and antinociceptive effects of fluoxetine in rats.

  2013cites this paper
• Effects of Palonosetron, a 5-HT3 Receptor Antagonist, on Mechanical Allodynia in a Rat Model of Postoperative Pain

  2013cites this paper
• Ondansetron pretreatment reduces pain on injection of propofol.

  2012cites this paper
• Role of peripheral and spinal 5-HT(3) receptors in development and maintenance of formalin-induced long-term secondary allodynia and hyperalgesia.

  2012cites this paper
• GABA, but not opioids, mediates the anti-hyperalgesic effects of 5-HT7 receptor activation in rats suffering from neuropathic pain.

  2012cites this paper
• α2-ADRENOCEPTOR AND 5-HT3 SEROTONIN RECEPTOR LIGANDS AS POTENTIAL ANALGESIC ADJUVANTS

  2012cites this paper
• Propofol reduces early post‐operative pain after gynecological laparoscopy

  2012cites this paper
• Differential adaptive changes on serotonin and noradrenaline transporters in a rat model of peripheral neuropathic pain.

  2012cites this paper
• Two methods of biomarker discovery: applications in neuropathic pain and pharmacotherapy.

  2011cites this paper
• Effect of Intravenous Ondansetron on Sensory and Motor Block after Spinal Anaesthesia with Hyperbaric Bupivacaine

  2011cites this paper
• The complex role of serotonin and 5-HT receptors in chronic pain

  2011cites this paper
• Pregabalin Suppresses Spinal Neuronal Hyperexcitability and Visceral Hypersensitivity in the Absence of Peripheral Pathophysiology

  2011cites this paper
• Further analyses of mechanisms underlying the antinociceptive effect of the triterpene 3β, 6β, 16β-trihydroxylup-20(29)-ene in mice.

  2011cites this paper
• Comparison among the effect of ondansetron, lidocaine and combination of ondansetron and lidocaine on microemulsion propofol injection pain

  2011cites this paper
• Lack of analgesic efficacy of spinal ondansetron on thermal and mechanical hypersensitivity following spinal nerve ligation in the rat.

  2010cites this paper
• Serotonin receptors are involved in the spinal mediation of descending facilitation of surgical incision-induced increase of Fos-like immunoreactivity in rats

  2010cites this paper
• 5-HT-induced depression of the spinal monosynaptic reflex potential utilizes different types of 5-HT receptors depending on Mg^2+ availability

  2009cites this paper
• Ondansetron added to lidocaine for intravenous regional anaesthesia

  2009cites this paper
• Responses to 5-HT in morphologically identified neurons in the rat substantia gelatinosa in vitro.

  2009cites this paper
• NK‐1 receptors in the rostral ventromedial medulla contribute to hyperalgesia produced by intraplantar injection of capsaicin

  2008cites this paper
• REVIEWARTICLES Spinal cord mechanisms of pain

  2008influential citation
• Spinal cord mechanisms of pain.

  2008cites this paper
• High Affinity Binding of Epibatidine to Serotonin Type 3 Receptors*

  2008cites this paper
• Analgesia mediated by the TRPM8 cold receptor in neuropathic pain

  2007cites this paper
• The neuronal 5-HT3 receptor network after 20 years of research--evolving concepts in management of pain and inflammation.

  2007cites this paper
• The Interaction between Intrathecal NMDA Receptor Antagonist and 5-HT3 Receptor Agonist in the Rat Formalin Test

  2007cites this paper
• Effects of the 5‐HT3 receptor antagonist, alosetron, in a rat model of somatic and visceral hyperalgesia

  2006cites this paper
• New treatment options using 5-HT3 receptor antagonists in rheumatic diseases.

  2006cites this paper
• Contribution of peripheral 5-HT2A or 5-HT3 receptors to Fos expression in the trigeminal spinal nucleus produced by acute injury to the masseter muscle during persistent temporomandibular joint inflammation in rats.

  2006cites this paper
• Tricyclic Antidepressants, SSRIs and Descending Serotinergic Facilitatory Pathways

  2006cites this paper
• Treatment options for chronic abdominal pain in children and adolescents

  2006cites this paper
• Serotonergic modulation of spinal sensory circuits.

  2006cites this paper
• Chapter 14 Descending excitatory systems.

  2006cites this paper
• Sérotonine et processus douloureux

  2006cites this paper
• Evidence of a role for descending serotonergic facilitation in a rat model of cancer-induced bone pain.

  2006cites this paper
• Do 5HT3 Antagonists Antinociceptive Have an Effect

  2005cites this paper
• The Use of the 5-HT3 Receptor Antagonist Tropisetron in Trigger Point Therapy: A Pilot Study

  2005cites this paper
• Antinociception: mechanistic studies on the action of MD-354 and clonidine. Part 1. The 5-HT3 component.

  2005cites this paper
• Central serotonin 3 receptors play an important role in the modulation of nociceptive neural activity of trigeminal subnucleus caudalis and nocifensive orofacial behavior in rats with persistent temporomandibular joint inflammation.

  2005cites this paper
• The 5HT3 Receptor Antagonist Ondansetron Can Have an Analgesic Effect in Chronic Human Neuropathic Pain

  2005cites this paper
• Ondansetron does not block tramadol-induced analgesia in mice.

  2005cites this paper
• Spinal and Supraspinal Contributions to Central Sensitization in Peripheral Neuropathy

  2005cites this paper
• Tricyclic Antidepressants, SSRIs and Descending Serotinergic Facilitatory Pathways: Could 5HT3 Antagonists Prevent Paradoxical Pain? A Hypothesis

  2005cites this paper
• Stimulation of the lateral hypothalamus produces antinociception mediated by 5-HT1A, 5-HT1B and 5-HT3 receptors in the rat spinal cord dorsal horn.

  2005cites this paper
• Anti-CD11d Integrin Antibody Treatment Restores Normal Serotonergic Projections to the Dorsal, Intermediate, and Ventral Horns of the Injured Spinal Cord

  2005cites this paper
• Possible involvement of opioidergic and serotonergic mechanisms in antinociceptive effect of paroxetine in acute pain.

  2004cites this paper
• Physiology and pathophysiology of the 5‐HT3 receptor

  2004cites this paper
• Asean Journal of Anaesthesiology

  2004cites this paper
• Serotonin type 3 receptor antagonist tropisetron in the treatment of chronic inflammatory rheumatic conditions – preliminary clinical experience

  2004cites this paper
• Blockade of peripheral 5HT3 receptor attenuates the formalin-induced nocifensive behavior in persistent temporomandibular joint inflammation of rat.

  2004cites this paper
• Descending facilitatory control of mechanically evoked responses is enhanced in deep dorsal horn neurones following peripheral nerve injury.

  2004cites this paper
• Local treatment of tendinopathies and myofascial painsyndromes with the 5‐HT3 receptor antagonist tropisetron

  2004cites this paper
• The influence of the 5‐HT3 receptor antagonist tropisetron on pain in fibromyalgia: a functional magnetic resonance imaging pilot study

  2004cites this paper
• Descending serotonergic facilitation mediated through rat spinal 5HT3 receptors is unaltered following carrageenan inflammation.

  2004cites this paper
• Effect of Ondansetron and Lidocaine on Vascular Pain Associated with Intravenous Propofol Injection

  2004cites this paper
• Analgesic effects of 5‐HT3 receptor antagonists

  2004cites this paper
• L-type calcium channel blockers enhance 5-HTP-induced antinociception in mice.

  2004cites this paper
• Does a Single Intravenous Injection of the 5HT3 Receptor Antagonist Ondansetron Have an Analgesic Effect in Neuropathic Pain? A Double-Blinded, Placebo-Controlled Cross-Over Study

  2003cites this paper
• The activation of 5-HT(3) receptors evokes GABA release in the spinal cord.

  2003cites this paper
• Absence of Thermal Hyperalgesia in Serotonin Transporter-Deficient Mice

  2003cites this paper
• Characterisation of axon terminals in the rat dorsal horn that are immunoreactive for serotonin 5-HT3A receptor subunits

  2003cites this paper
• Synergistic enhancement of glutamate-mediated responses by serotonin and forskolin in adult mouse spinal dorsal horn neurons.

  2002cites this paper
• Olanzapine in the management of cancer pain.

  2002cites this paper
• Replicate high-density rat genome oligonucleotide microarrays reveal hundreds of regulated genes in the dorsal root ganglion after peripheral nerve injury.

  2002cites this paper