High efficiency mutagenesis, repair, and engineering of chromosomal DNA using single-stranded oligonucleotides

No abstract is available for this paper.

• Publication year

  2001

• Venue

  Proceedings of the National Academy of Sciences of the United States of America

• Publication date

  2001-05-29

• Fields of study

  Biology, Medicine, Engineering

• Identifiers
  DOI 10.1073/pnas.121164898PMID 11381128PMCID PMC34423
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA.

  2001cited by this paper
• RecE/RecT and Redα/Redβ initiate double-stranded break repair by specifically interacting with their respective partners

  2000cited by this paper
• Rapid engineering of bacterial artificial chromosomes using oligonucleotides

  2000cited by this paper
• A Single Nuclease Active Site of the Escherichia coli RecBCD Enzyme Catalyzes Single-stranded DNA Degradation in Both Directions*

  2000cited by this paper
• The DNA damage response: putting checkpoints in perspective

  2000cited by this paper
• RecE/RecT and Redalpha/Redbeta initiate double-stranded break repair by specifically interacting with their respective partners.

  2000cited by this paper
• An efficient recombination system for chromosome engineering in Escherichia coli.

  2000cited by this paper
• Recombinational Repair of DNA Damage inEscherichia coli and Bacteriophage λ

  1999cited by this paper
• Recombinational repair of DNA damage in Escherichia coli and bacteriophage lambda.

  1999cited by this paper
• Rapid modification of bacterial artificial chromosomes by ET-recombination.

  1999cited by this paper
• Rings and filaments of beta protein from bacteriophage lambda suggest a superfamily of recombination proteins.

  1999cited by this paper
• Rings and filaments of β protein from bacteriophage λ suggest a superfamily of recombination proteins

  1999cited by this paper
• Binding of double-strand breaks in DNA by human Rad52 protein

  1999cited by this paper
• Use of Bacteriophage λ Recombination Functions To Promote Gene Replacement in Escherichia coli

  1998cited by this paper
• The beta protein of phage lambda binds preferentially to an intermediate in DNA renaturation.

  1998cited by this paper
• Sequence Analysis of Tn10 Insertion Sites in a Collection of Escherichia coli Strains Used for Genetic Mapping and Strain Construction

  1998cited by this paper
• A new logic for DNA engineering using recombination in Escherichia coli

  1998cited by this paper
• The beta protein of phage lambda promotes strand exchange.

  1998cited by this paper
• Characterization of the DNA-binding domain of beta protein, a component of phage lambda red-pathway, by UV catalyzed cross-linking.

  1996cited by this paper
• Escherichia coli and Salmonella.

  1996cited by this paper
• A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae.

  1993cited by this paper
• Strand-specificity in the transformation of yeast with synthetic oligonucleotides.

  1992cited by this paper
• Parameters affecting the frequencies of transformation and co‐transfromation with synthetic oligonucleotides in yeast

  1992cited by this paper
• Lambda Gam protein inhibits the helicase and chi-stimulated recombination activities of Escherichia coli RecBCD enzyme

  1991cited by this paper
• Interaction between the sbcC gene of Escherichia coli and the gam gene of phage lambda.

  1989cited by this paper
• Transformation of yeast with synthetic oligonucleotides.

  1988cited by this paper
• The homologous recombination system of phage lambda. Pairing activities of beta protein.

  1986cited by this paper
• Deletion of an essential gene in Escherichia coli by site-specific recombination with linear DNA fragments

  1984cited by this paper
• Beta protein of bacteriophage lambda promotes renaturation of DNA.

  1981cited by this paper
• The gamma protein specified by bacteriophage gamma. Structure and inhibitory activity for the recBC enzyme of Escherichia coli.

  1975cited by this paper
• Transfection of Escherichia coli spheroplasts. V. Activity of recBC nuclease in rec+ and rec minus spheroplasts measured with different forms of bacteriophage DNA

  1975cited by this paper
• An exonuclease induced by bacteriophage lambda. II. Nature of the enzymatic reaction.

  1967cited by this paper

• Transcription terminators can be strong promoters

  2026cites this paper
• Exploration of Retron Elements for the Genetic Engineering of Acidithiobacillus ferrooxidans.

  2025cites this paper
• An Undergraduate Laboratory on Recombineering and CRISPR/Cas9‐Assisted Gene Editing in Escherichia coli

  2025cites this paper
• Evolutionary trajectories determine feasibility of collateral sensitivity-based antibiotic treatment strategies in critical bacterial pathogens

  2025cites this paper
• Crystal Structure and Functional Characterization of YjgK From Salmonella Typhimurium

  2025cites this paper
• Efficient high-precision transgene knock-in by Recombinases (Redα/β)-enhanced DNA integration-CRISPR-Cas9 (RED-CRISPR)

  2025cites this paper
• A diverse single-stranded DNA–annealing protein library enables efficient genome editing across bacterial phyla

  2025cites this paper
• Inducible genome-wide mutagenesis for improvement of pDNA production by E. coli

  2025cites this paper
• Genome-Editing Tools for Lactic Acid Bacteria: Past Achievements, Current Platforms, and Future Directions

  2025cites this paper
• An automated platform for accelerating and focusing adaptive laboratory evolution.

  2025cites this paper
• Harnessing CRISPR-Cas9 for Lactobacillus improvement in silage production: current knowledge and future perspectives

  2025cites this paper
• Unraveling N-Terminal Proteoform Interactomes via Multiplexed Recombineering in Salmonella.

  2025cites this paper
• iCASRED, a scarless DNA editing tool in E. coli for high-efficiency engineering of natural product biosynthetic gene clusters

  2025cites this paper
• A comprehensive review of genomic-scale genetic engineering as a strategy to improve bacterial productivity

  2025cites this paper
• Engineering a genomically recoded organism with one stop codon

  2025cites this paper
• Genome editing of phylogenetically distinct bacteria using portable retron-mediated recombineering

  2025cites this paper
• Genomic Modifications of Lactic Acid Bacteria and Their Applications in Dairy Fermentation.

  2024cites this paper
• In vivo assembly of genetic constructs in filamentous fungus Talaromyces cellulolyticus.

  2024cites this paper
• The metabolic slowdown caused by the deletion of pspA accelerates protein aggregation during stationary phase facilitating antibiotic persistence

  2024cites this paper
• Cracking the Code: Reprogramming the Genetic Script in Prokaryotes and Eukaryotes to Harness the Power of Noncanonical Amino Acids

  2024cites this paper
• The herpes simplex virus alkaline nuclease is required to maintain replication fork progression

  2024cites this paper
• The recombinant vaccine of Lactobacillus plantarum elicits immune protection against H1N1 and H9N2 influenza virus infection.

  2024cites this paper
• Development of a Recombineering System for the Acetogen Eubacterium limosum with Cas9 Counterselection for Markerless Genome Engineering

  2024cites this paper
• Structural basis for the interaction of Redβ single strand annealing protein with Escherichia coli single-stranded DNA binding protein.

  2024cites this paper
• The design and engineering of synthetic genomes

  2024cites this paper
• Reducing competition between msd and genomic DNA improves retron editing efficiency

  2024influential citation
• Increasing the efficiency of gene editing with CRISPR-Cas9 via concurrent expression of the Beta protein.

  2024cites this paper
• Proof of ssDNA degraded from dsDNA for ET recombination

  2024cites this paper
• Reducing competition between msd and genomic DNA significantly improved the editing efficiency of the retron editing system

  2024cites this paper
• Engineered bacteriophages: A panacea against pathogenic and drug resistant bacteria

  2024cites this paper
• Coselection of BAC for Escherichia coli chromosomal DNA multiplex automated genome engineering

  2024influential citation
• ReaL-MGE is a tool for enhanced multiplex genome engineering and application to malonyl-CoA anabolism

  2024cites this paper
• Employing Synthetic Biology to Expand Antibiotic Discovery.

  2024cites this paper
• Markerless bacterial artificial chromosome manipulation method by red proteins of phage λ mediated homologous recombination utilizing fluorescent proteins for both positive and counter selection

  2023cites this paper
• High-resolution landscape of an antibiotic binding site

  2023cites this paper
• The emerging role of recombineering in microbiology

  2023cites this paper
• A study on L-threonine and L-serine uptake in Escherichia coli K-12

  2023cites this paper
• Continuous Multiplexed Phage Genome Editing Using Recombitrons

  2023cites this paper
• DNA Recombineering Applications.

  2023cites this paper
• Recent advances in biological approaches towards anode biofilm engineering for the improvement of extracellular electron transfer in the microbial fuel cell

  2023cites this paper
• Half a century after their discovery: Structural insights into exonuclease and annealase proteins catalyzing recombineering

  2023cites this paper
• Scarless DNA Recombineering.

  2023cites this paper
• ORBIT for E. coli: kilobase-scale oligonucleotide recombineering at high throughput and high efficiency

  2023cites this paper
• Comparison of phage-derived recombinases for genetic manipulation of Pseudomonas species

  2023cites this paper
• CRISPR/Cas9-assisted ssDNA recombineering for site-directed mutagenesis and saturation mutagenesis of plasmid-encoded genes

  2023cites this paper
• An expanded CRISPR–Cas9-assisted recombineering toolkit for engineering genetically intractable Pseudomonas aeruginosa isolates

  2023cites this paper
• Metagenomics harvested genus-specific single-stranded DNA-annealing proteins improve and expand recombineering in Pseudomonas species

  2023cites this paper
• Unpredictability of the Fitness Effects of Antimicrobial Resistance Mutations Across Environments in Escherichia coli

  2023cites this paper
• Recombineering: Genetic Engineering in Escherichia coli Using Homologous Recombination

  2023cites this paper
• CRISPR for Rewriting Genetic Code

  2022cites this paper
• MIT Open Access

  2022cites this paper
• Enhancement of RecET-mediated in vivo linear DNA assembly by a xonA mutation

  2022cites this paper
• High fidelity one‐pot DNA assembly using orthogonal serine integrases

  2022cites this paper
• Recombineering in Non‐Model Bacteria

  2022cites this paper
• Homologs of the Escherichia coli F Element Protein TraR, Including Phage Lambda Orf73, Directly Reprogram Host Transcription

  2022cites this paper
• Past, Present, and Future of Genome Modification in Escherichia coli

  2022cites this paper
• CRISPR/Cas9‐enhanced Targetron Insertion for Delivery of Heterologous Sequences into the Genome of Gram‐Negative Bacteria

  2022cites this paper
• Redβ177 annealase structure reveals details of oligomerization and λ Red-mediated homologous DNA recombination

  2022cites this paper
• A simple approach for random genomic insertion–deletions using ambiguous sequences in Escherichia coli

  2022cites this paper
• Advanced genome-editing technologies enable rapid and large-scale generation of genetic variants for strain engineering and synthetic biology.

  2022cites this paper
• High-Efficiency Multi-site Genomic Editing (HEMSE) Made Easy.

  2022cites this paper
• Generating Single Nucleotide Point Mutations in E. coli with the No-SCAR System.

  2022cites this paper
• Robust counterselection and advanced λRed recombineering enable markerless chromosomal integration of large heterologous constructs

  2022cites this paper
• Recombineering in Staphylococcus aureus.

  2022cites this paper
• SURE editing: combining oligo-recombineering and programmable insertion/deletion of selection markers to efficiently edit the Mycoplasma pneumoniae genome

  2022influential citation
• Efficient and iterative retron-mediated in vivo recombineering in Escherichia coli

  2022influential citation
• Strategies to identify and edit improvements in synthetic genome segments episomally

  2022cites this paper
• Genome engineering of the Corynebacterium glutamicum chromosome by the Extended Dual-In/Out strategy.

  2022cites this paper
• Development and optimization of Lysis gene E as a counter‐selection marker with high stringency

  2022cites this paper
• Development and optimization of Lysis gene E as a counter-selection marker with high selection stringency

  2021cites this paper
• Approaches to genetic tool development for rapid domestication of non-model microorganisms

  2021cites this paper
• High-throughput genetic engineering tools for regulating gene expression in a microbial cell factory

  2021cites this paper
• Expression of the qepA1 gene is induced under antibiotic exposure

  2021cites this paper
• Oligo Pools as an Affordable Source of Synthetic DNA for Cost‐Effective Library Construction in Protein‐ and Metabolic Pathway Engineering

  2021cites this paper
• Microfluidic screening and genomic mutation identification for enhancing cellulase production in Pichia pastoris

  2021cites this paper
• Construction of Novel Live Genetically Modified BCG Vaccine Candidates Using Recombineering Tools.

  2021cites this paper
• Improved dsDNA recombineering enables versatile multiplex genome engineering of kilobase-scale sequences in diverse bacteria

  2021cites this paper
• Engineering Wired Life: Synthetic Biology for Electroactive Bacteria.

  2021cites this paper
• Investigation of the Phenotypic Effect of Mutating a Highly-Conserved Cysteine Residue in the RNA Polymerase Beta Prime Subunit of E. Coli RNA Polymerase

  2021cites this paper
• Bacteriophage: Red Recombination System and the Development of Recombineering Technologies

  2021cites this paper
• ReScribe: An Unrestrained Tool Combining Multiplex Recombineering and Minimal-PAM ScCas9 for Genome Recoding Pseudomonas putida

  2021cites this paper
• Mutational Analysis of Redβ Single Strand Annealing Protein: Roles of the 14 Lysine Residues in DNA Binding and Recombination In Vivo

  2021cites this paper
• Host-parasite coevolution promotes innovation through deformations in fitness landscapes

  2021cites this paper
• Rapid Genome Modification in Serratia marcescens Through Red Homologous Recombination

  2021cites this paper
• Recombineering and MAGE

  2021cites this paper
• Expanding Diversity of Firmicutes Single-Strand Annealing Proteins: A Putative Role of Bacteriophage-Host Arms Race

  2021cites this paper
• Chemical-genetic interrogation of RNA polymerase mutants reveals structure-function relationships and physiological tradeoffs.

  2021cites this paper
• Engineered Bacteriophage Therapeutics: Rationale, Challenges and Future

  2021cites this paper
• Directed Evolution: Methodologies and Applications.

  2021cites this paper
• Metabolic Engineering of Lactic Acid Bacteria

  2021cites this paper
• Phenotypic and genetic barriers to establishment of horizontally transferred genes encoding ribosomal protection proteins

  2021cites this paper
• Optimizing recombineering in Corynebacterium glutamicum

  2021cites this paper
• Oligomeric complexes formed by Redβ single strand annealing protein in its different DNA bound states

  2021cites this paper
• Herpes ICP8 protein stimulates homologous recombination in Herpes ICP8 protein stimulates homologous recombination in human cells [preprint] human cells [preprint] how benefits you.

  2021cites this paper
• Comparing treatment strategies to reduce antibiotic resistance in an in vitro epidemiological setting

  2021cites this paper
• A multifunctional system for genome editing and large-scale interspecies gene transfer

  2021cites this paper
• A navigation guide of synthetic biology tools for Pseudomonas putida.

  2021cites this paper
• Mismatch Intolerance of 5′-Truncated sgRNAs in CRISPR/Cas9 Enables Efficient Microbial Single-Base Genome Editing

  2021cites this paper
• Improved bacterial recombineering by parallelized protein discovery

  2020cites this paper
• Characterizing the portability of phage-encoded homologous recombination proteins

  2020cites this paper