Sodium gradient-dependent calcium uptake in renal basolateral membrane vesicles. Effect of parathyroid hormone.

The Na+/Ca2+ exchange system in rat renal cortex basolateral membrane vesicles was studied. Uptake and efflux of Ca2+ in the membrane vesicles were stimulated by trans-Na+. The enhancement of Ca2+ uptake by the intravesicular greater than extravesicular Na+ gradient was inhibited by ionophores that dissipated the gradient, and was increased by an outside negative membrane potential. Na+-dependent Ca2+ uptake was saturable with respect to both Ca2+ and Na+. A [Ca2+]0.5 of 8 microM was calculated. The relationship between Na+ concentration and rate of Ca2+ efflux was sigmoidal; a [Na+]0.5 of 15 mM and a Hill coefficient of 2.5 were estimated. Removal of parathyroid glands from the rats resulted in a 40% decrease in the Na+-dependent Ca2+ uptake. Infusion of parathyroid hormone (the synthetic tetratriacontapeptide) into these animals fully restored the activity. The isolated basolateral membrane possessed parathyroid hormone-sensitive adenylate cyclase. These findings may suggest a mechanism by which parathyroid hormone regulates the reabsorption of Ca2+ in the kidney.

• Publication year

  1984

• Venue

  Journal of Biological Chemistry

• Publication date

  1984-09-10

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/s0021-9258(18)90587-9PMID 6469984
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Glutamine transport in renal basolateral vesicles from dogs with metabolic acidosis.

  1984cited by this paper
• Competitive interactions of sodium and calcium with the sodium-calcium exchange system of cardiac sarcolemmal vesicles.

  1983cited by this paper
• High-affinity Ca-Mg-ATPase along the rabbit nephron.

  1982cited by this paper
• Metabolic heterogeneity of the nephron.

  1982cited by this paper
• Cytosolic free calcium levels in rabbit proximal kidney tubules.

  1982cited by this paper
• Na+-Ca2+ exchange in inside-out cardiac sarcolemmal vesicles.

  1982cited by this paper
• Localization and properties of a high‐affinity (Ca2+ + Mg2+)‐ATPase in isolated kidney cortex plasma membranes

  1982cited by this paper
• A simple isolation method for basal-lateral plasma membranes from rat kidney cortex.

  1981cited by this paper
• Distribution of hormone-dependent adenylate cyclase in the nephron and its physiological significance.

  1981cited by this paper
• Calmodulin stimulation of renal (Ca2+ + Mg2+)‐ATPase

  1981cited by this paper
• Calcium transport mechanisms in membrane vesicles from guinea pig brain synaptosomes.

  1981cited by this paper
• Adenylate cyclase and cell cyclic AMP of rat cortical thick ascending limb of Henle.

  1981cited by this paper
• Stimulation of the efflux of L-glutamate from renal brush-border membrane vesicles by extravesicular potassium

  1981cited by this paper
• Sodium gradient- and sodium plus potassium gradient-dependentl-glutamate uptake in renal basolateral membrane vesicles

  1981cited by this paper
• Sodium-calcium interactions in the renal proximal convoluted tubule of the rabbit.

  1981cited by this paper
• Calcium transport in the thick ascending limb of Henle. Heterogeneity of function in the medullary and cortical segments.

  1980cited by this paper
• Cytosolic calcium ion activity in epithelial cells of Necturus kidney

  1980cited by this paper
• Effect of PTH on calcium transport across the cortical thick ascending limb of Henle's loop.

  1980cited by this paper
• Effects of PTH, ADH, and cyclic AMP on distal tubular Ca and Na reabsorption.

  1980cited by this paper
• Sodium-calcium exchange and sidedness of isolated cardiac sarcolemmal vesicles.

  1980cited by this paper
• Calcium ion transport across plasma membranes isolated from rat kidney cortex.

  1979cited by this paper
• Calcium transport in the nephron.

  1979cited by this paper
• Isolation of luminal and antiluminal membranes from dog kidney cortex.

  1979cited by this paper
• Stoichiometry of sodium-calcium exchange in cardiac sarcolemmal vesicles. Coupling to the sodium pump.

  1979cited by this paper
• Calcium transport across segments of the rabbit distal nephron in vitro.

  1978cited by this paper
• Role of calcium in the decline of phosphate reabsorption during phosphate loading in acutely thyroparathyroidectomized rats

  1978cited by this paper
• Distal site of calcium reabsorption in the rat nephron

  1978cited by this paper
• Potentiation of GTP of hormone-responsive adenylate cyclase in renal cortex plasma membrane preparations.

  1978cited by this paper
• Preparation of renal cortex basal-lateral and bursh border membranes. Localization of adenylate cyclase and guanylate cyclase activities.

  1977cited by this paper
• Active Ca2+ reabsorption in the proximal tubule of the rat kidney

  1976cited by this paper
• The Na+ gradient-dependent transport of D-glucose in renal brush border membranes.

  1975cited by this paper
• Energetics of the Na+-dependent transport of D-glucose in renal brush border membrane vesicles.

  1975cited by this paper
• Further studies on the previously proposed saturable high affinity site for D-glucose in renal brush border membrane preparations.

  1974cited by this paper
• Thyrocalcitonin, EGTA, and urinary electrolyte excretion.

  1967cited by this paper
• STUDIES OF CALCIUM AND PHOSPHORUS METABOLISM: III. The Effects of the Thyroid Hormone and Thyroid Disease.

  year unknowncited by this paper

• The effect of calcineurin inhibition on the expression and activation of renal electrolyte transporters

  2017cites this paper
• Dilated Cardiomyopathy due to Hypocalcemia Secondary to Congenital Hypoparathyroidism in a Newborn with DiGeorge Syndrome

  2017cites this paper
• Regulation of Calcium Homeostasis and Genetic Disorders that Affect Calcium Metabolism∗∗Chapter titles shaded in green indicate chapters dedicated predominantly to pediatric endocrinology conten

  2016cites this paper
• exchanger expression in distal convoluted tubule cells

  2016cites this paper
• Distal convoluted tubule.

  2015cites this paper
• [A reversible cause of dilated cardiomyopathy: hypocalcemia].

  2014cites this paper
• Renal Calcium Metabolism

  2013cites this paper
• Use of recombinant human parathyroid hormone in hypocalcemic cardiomyopathy.

  2012cites this paper
• Newborn with Dilated Cardiomyopathy Secondary to Vitamin D Deficiency

  2012cites this paper
• Newborn with dilated cardiomyopathy secondary to vitamin d deficiency.

  2012cites this paper
• A case of hypocalcemia-induced dilated cardiomyopathy.

  2010cites this paper
• ENDOCRINE REGULATION OF CALCIUM TRANSPORT IN EPITHELIA

  2008cites this paper
• CHAPTER 65 – Renal Calcium Metabolism

  2008cites this paper
• PTH and PTHrP Actions on Kidney and Bone

  2008cites this paper
• Parathyroid hormone-induced changes of the brush border topography and cytoskeleton in cultured renal proximal tubular cells

  2005cites this paper
• Calcium absorption across epithelia.

  2005cites this paper
• Calcium absorption by fish intestine: The involvement of ATP-and sodium-dependent calcium extrusion mechanisms

  2005cites this paper
• Marinobufagenin may mediate the adverse impact of salty diets on renal calcium retention by impairing the efficiency of renal tubular sodium-calcium exchange.

  2005cites this paper
• Mechanisms of calcium transport across the basolateral membrane of the rabbit cortical thick ascending limb of Henle's loop

  2004cites this paper
• Mechanisms of Calcium Transport in Human Colonic Basolateral Membrane Vesicles

  2002cites this paper
• Plasma membrane Ca2+-ATPase and NCX1 Na+/Ca2+ exchanger expression in distal convoluted tubule cells.

  2002cites this paper
• Physiological characterization of the Na(+)/Ca(2+) exchanger (NCX) in hepatopancreatic and antennal gland basolateral membrane vesicles isolated from the freshwater crayfish Procambarus clarkii.

  2002cites this paper
• Characterization of Na/Ca exchange in plasmalemmal vesicles from zona fasciculata cells of the bovine adrenal gland.

  2000cites this paper
• Mammalian distal tubule: physiology, pathophysiology, and molecular anatomy.

  2000cites this paper
• Sodium/calcium exchange: its physiological implications.

  1999cites this paper
• Energized Ca2+ transport by hepatopancreatic basolateral plasma membranes of Homarus americanus.

  1998cites this paper
• Na+/Ca2+ exchanger in epithelial cells of the porcine cortical thick ascending limb.

  1996cites this paper
• Role of secondary hyperparathyroidism in the development of post-transplant acute tubular necrosis.

  1996cites this paper
• Metabolic and energetic properties of isolated nerve ending particles (synaptosomes).

  1996cites this paper
• Ca2+, Mg2+, and Phosphate Metabolism

  1996cites this paper
• Vitamin D and intestinal calcium transport: facts, speculations and hypotheses.

  1995cites this paper
• Basic Concepts of Renal Physiology

  1995cites this paper
• Na/Ca exchange in the basolateral membrane of the A6 cell monolayer: role in Cai homeostasis

  1995cites this paper
• Cellular calcium transport in renal epithelia: measurement, mechanisms, and regulation.

  1995cites this paper
• Na+/Ca2+ Exchange, Ca2+ Binding, and Electrogenic Ca2+ Transport in Plasma Membranes of Human Placental Syncytiotrophoblast

  1994cites this paper
• Functional expression of human renal Na+/Ca2+ exchanger in insect cells.

  1994cites this paper
• Immunocytochemical localization of sodium-calcium exchanger in canine nephron.

  1993cites this paper
• Immunolocalization of the Na+/Ca2+ exchanger in rabbit kidney.

  1993cites this paper
• Decrease in Gs protein expression may impair adenylate cyclase activation in old kidneys.

  1993cites this paper
• Calcium transport by basolateral membranes of diabetic rats.

  1993cites this paper
• ATP-dependent strontium uptake by basolateral membrane vesicles from rat renal cortex in the absence or presence of calcium

  1992cites this paper
• Cellular Calcium Transport Systems

  1992cites this paper
• The renal sodium-calcium exchanger.

  1992cites this paper
• Parathyroid hormone stimulates ATP-dependent calcium pump activity by a different mode in proximal and distal tubules of the rat.

  1992cites this paper
• Na(+)-Ca2+ exchanger of rat proximal tubule: gene expression and subcellular localization.

  1992cites this paper
• Rapid Ca2+ extrusion via the Na+/Ca2+ exchanger of the human platelet

  1992cites this paper
• Identification and localization of renal Na(+)-Ca2+ exchanger by polymerase chain reaction.

  1992cites this paper
• Potassium channels in renal epithelial transport regulation.

  1992cites this paper
• The Effects of Parathyroid Hormone (PTH) on Ca Transport

  1991cites this paper
• Na(+)-Ca2+ exchange and Ca2+ depletion in rat proximal tubules.

  1991cites this paper
• The mechanism of parathyroid hormone action on calcium reabsorption by the distal tubule.

  1991cites this paper
• Mechanism of sodium transport inhibition by epidermal growth factor in cortical collecting ducts.

  1991cites this paper
• Intrinsic regulation of apical sodium entry in epithelia.

  1991cites this paper
• The Role of Na ‐ Ca Exchange in Renal Epithelia

  1991cites this paper
• Role of Ca2+/H+ antiporter in the kidney.

  1991cites this paper
• Renal Tubular Transport of Calcium

  1991cites this paper
• Renal Acidification: Cellular Mechanisms of Tubular Transport and Regulation

  1991cites this paper
• Endocrine Function and Aging

  1990cites this paper
• cAMP-stimulated rise of [Ca2+]i in rabbit connecting tubules: role of peritubular Ca.

  1990cites this paper
• Downregulation of parathyroid hormone receptors in renal membranes from aged rats.

  1990cites this paper
• Renal control of extracellular calcium.

  1990cites this paper
• Stimulation of ATP-driven Ca2+ pump in the basal-lateral plasma membranes of kidney cortex during compensatory renal growth.

  1990cites this paper
• Regulation of renal sodium calcium exchange by PTH: alteration with age.

  1990cites this paper
• Effect of Age on PTH-Stimulated Sodium Calcium Exchange Activity: Alteration at Cellular and Molecular Levels

  1990cites this paper
• Parathyroid hormone regulation of cytosolic Ca2+ in rat proximal tubules.

  1990cites this paper
• Desensitization to parathyroid hormone in renal cells from aged rats is associated with alterations in G-protein activity.

  1989cites this paper
• PTH Increases Ca++ Transport in Rat Brain Synaptosomes in Uremia

  1989cites this paper
• Polyamine transport systems in the LLC-PK1 renal epithelial established cell line.

  1989cites this paper
• The renal Na+/Ca2+ exchange system is located exclusively in the distal tubule.

  1989cites this paper
• Na/Ca exchangers in collecting cells of rat kidney

  1989cites this paper
• Na+ electrochemical gradient and Na+-Ca2+ exchange in rat proximal tubule.

  1989cites this paper
• Parathyroid hormone mediates changes in calcium transport in uremic rat brain synaptosomes.

  1988cites this paper
• Characterization of calcium transport by basolateral membrane vesicles of human small intestine.

  1988cites this paper
• ATP-driven Ca2+ pump in the basolateral membrane of rat kidney cortex catalyzes an electroneutral Ca2+/H+ antiport.

  1988cites this paper
• Calcium transport mechanisms in epithelial cell membranes.

  1988cites this paper
• NaCl Transport in the Kidney

  1988cites this paper
• Mechanism of action of thiazide diuretics.

  1988cites this paper
• NaCl Transport in Epithelia

  1988cites this paper
• Na+-Ca2+ exchange and calcium permeability in canine basolateral membrane vesicles: the effects of dibutyryl cAMP and specific inhibitors.

  1988cites this paper
• Potential role of cytoplasmic calcium ions in the regulation of sodium transport in renal tubules.

  1988cites this paper
• Dichlorobenzamil inhibits stimulated bone resorption in vitro.

  1988cites this paper
• Na+-Ca2+ exchange in plasma membranes of crayfish striated muscle.

  1987cites this paper
• Transcellular calcium transport in intestinal and renal epithelial cells.

  1987cites this paper
• Partial characterization of two (Ca+2 + Mg+2)-dependent ATPase activities from bovine brain synaptic membrane homogenates.

  1987cites this paper
• Renal basolateral membrane Na-Ca exchange is electrogenic.

  1987cites this paper
• Angiotensin converting enzyme inhibition and acute tubular necrosis.

  1987cites this paper
• Transcellular calcium transport in intestinal and renal epithelial cells.

  1987cites this paper
• Renal electrolyte excretion and renin release during calcium and parathormone infusions in conscious rabbits.

  1986cites this paper
• Ca2+-dependent ATPases in the basolateral membrane of rat kidney cortex.

  1986cites this paper
• Renal membrane transport of calcium.

  1986cites this paper
• Liver plasma membrane calcium transport. Evidence for a Na+-dependent Ca2+ flux.

  1986cites this paper
• Intact parathyroid hormone (1-84) in primary hypertension.

  1986cites this paper
• ATP-dependent calcium transport in rat parotid basolateral membrane vesicles. Modulation by agents which elevate cyclic AMP.

  1986cites this paper
• Transport studies in plasma membrane vesicles isolated from renal cortex.

  1986cites this paper
• Inositol 1,4,5-trisphosphate releases Ca2+ from a nonmitochondrial store site in permeabilized rat cortical kidney cells.

  1986cites this paper
• Parathyroid hormone increases sodium/calcium exchange activity in renal cells and the blunting of the response in aging.

  1986cites this paper
• Hormonal control of kidney functions at the cell level.

  1986cites this paper
• Calcium transport abnormality in uremic rat brain synaptosomes.

  1985cites this paper
• ATP-dependent Ca2+ transport and Na+/Ca2+ exchange system in renal basolateral plasmamembranes of spontaneously hypertensive rats.

  1985cites this paper
• Characterization of ATP-driven calcium uptake in renal basal-lateral and renal endoplasmic reticulum membrane vesicles.

  1985cites this paper