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Combined Infection Control and Enhanced Osteogenic Differentiation Capacity on Additive Manufactured Ti‐6Al‐4V are Mediated via Titania Nanotube Delivery of Novel Biofilm Inhibitors

Jun Li,I. Mutreja,G. Hooper,K. Clinch,K. Lim,G. Evans,T. Woodfield

Published 2020 in Advanced Materials Interfaces

ABSTRACT

Additive manufacturing (AM) of titanium alloys offers the capacity to fabricate patient‐specific implants with defined porous architecture to enhance bone‐implant fixation. However, clinical challenges associated with orthopedic implants include inconsistent osseointegration and biofilm‐associated peri‐implant infection, leading to implant failure. Here, a strategy is developed to reduce infection and promote osteogenesis simultaneously on AM Ti‐6Al‐4V implants by delivering biofilm inhibitor molecules via titania nanotube surface modification. Electrochemical anodization is performed on polished and as‐manufactured Ti‐6Al‐4V to generate nanotubes, which are utilized for delivery of a novel methylthioadenosine nucleosidase inhibitor (MTANi) that targets MTAN—a key enzyme in bacterial metabolism involved in biofilm formation—thereby offering biofilm inhibition capacity combined with surface nano‐topography for promoting osteogenesis. Clinical isolates of staphylococcus cohnii formed firm biofilms on polished and AM Ti‐6Al‐4V controls whereas modified implants loaded with MTANi inhibit biofilm formation. Anodized AM Ti‐6Al‐4V nanotube substrates enhance alkaline phosphatase production, bone‐specific protein expression (osteocalcin, collagen I) and mineral deposition of human mesenchymal stromal cells (hMSCs), compared to as‐manufactured controls. Importantly, no detrimental effects on hMSC proliferation and osteogenic differentiation are observed for MTANi‐loaded substrates. Application of novel MTANi and electrochemical anodization offers a promising strategy for titanium alloy implant surface modification.

PUBLICATION RECORD

  • Publication year

    2020

  • Venue

    Advanced Materials Interfaces

  • Publication date

    2020-02-20

  • Fields of study

    Medicine, Materials Science, Engineering

  • Identifiers
    DOI 10.1002/admi.201901963
  • External record

    Open on Semantic Scholar

  • Source metadata

    Semantic Scholar

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