How do Dachsous and Fat polarise cells in the larval abdomen of Drosophila

We investigate the mechanisms of planar cell polarity (PCP) in the larval epidermis of Drosophila. Measurements of the amount of Dachsous across the segment find a peak located near the rear of the anterior compartment. Localisation of Dachs and orientation of ectopic denticles reveal the polarity of every cell in the segment. We discuss how well these findings evidence a zigzag gradient model of Dachsous activity. Several groups have proposed that Dachsous and Fat fix the direction of PCP via oriented microtubules that transport PCP proteins to one side of the cell. We test this proposition in the larval cells and find that most microtubules grow perpendicularly to the axis of PCP. We find no significant bias in the polarity of those microtubules aligned close to that axis. We also reexamine published data from the pupal abdomen and fail to find evidence supporting the hypothesis that microtubular orientation draws the arrow of PCP.

• Publication year

  2020

• Venue

  bioRxiv

• Publication date

  2020-02-21

• Fields of study

  Biology, Chemistry

• Identifiers
  DOI 10.1101/2020.02.21.959213
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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