The Role of Lipopeptidophosphoglycan in the Immune Response to Entamoeba histolytica

The sensing of Pathogen Associated Molecular Patterns (PAMPs) by innate immune receptors, such as Toll-like receptors (TLRs), is the first step in the inflammatory response to pathogens. Entamoeba histolytica, the etiological agent of amebiasis, has a surface molecule with the characteristics of a PAMP. This molecule, which was termed lipopeptidophosphoglycan (LPPG), is recognized through TLR2 and TLR4 and leads to the release of cytokines from human monocytes, macrophages, and dendritic cells; LPPG-activated dendritic cells have increased expression of costimulatory molecules. LPPG activates NKT cells in a CD1d-dependent manner, and this interaction limits amebic liver abscess development. LPPG also induces antibody production, and anti-LPPG antibodies prevent disease development in animal models of amebiasis. Because LPPG is recognized by both the innate and the adaptive immune system (it is a “Pamptigen”), it may be a good candidate to develop a vaccine against E. histolytica infection and an effective adjuvant.

• Publication year

  2010

• Venue

  Journal of Biomedicine and Biotechnology

• Publication date

  2010-01-21

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1155/2010/254521PMID 20145703PMCID 2817369
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Lack of antibody affinity maturation due to poor Toll-like receptor stimulation leads to enhanced respiratory syncytial virus disease

  2010cited by this paper
• Toxoplasma gondii glycosylphosphatidylinositols up-regulate major histocompatibility complex (MHC) molecule expression on primary murine macrophages

  2009influential reference
• Lack of antibody affinity maturation due to poor Toll-like receptor stimulation leads to enhanced respiratory syncytial virus disease

  2009cited by this paper
• TLR2 and TLR4 signaling shapes specific antibody responses to Salmonella typhi antigens

  2009influential reference
• Towards an understanding of the adjuvant action of aluminium

  2009cited by this paper
• Natural Killer T Cells Activated by a Lipopeptidophosphoglycan from Entamoeba histolytica Are Critically Important To Control Amebic Liver Abscess

  2009influential reference
• Response to Comment on “TLR9-Dependent Activation of Dendritic Cells by DNA from Leishmania major Favors Th1 Cell Development and the Resolution of Lesions”

  2009cited by this paper
• Immunostimulatory activity of haptenated proteins

  2009cited by this paper
• Entamoeba histolytica EhDEAD1 is a conserved DEAD-box RNA helicase with ATPase and ATP-dependent RNA unwinding activities.

  2008cited by this paper
• [Amebiasis: importance of the diagnosis and treatment. Minireview].

  2008cited by this paper
• Amebiasis.

  2008cited by this paper
• Innate immunity prevents tissue invasion by Entamoeba histolytica.

  2008cited by this paper
• Historia del protozoo Entamoeba histolytica

  2008cited by this paper
• [History of the Entamoeba histolytica protozoan].

  2008cited by this paper
• Análisis de factores asociados al fracaso del tratamiento médico del absceso hepático amebiano

  2008cited by this paper
• Amibiasis: Importancia de su diagnóstico y tratamiento. Mini-revisión

  2008cited by this paper
• Abscesos amebianos de hígado: Tres años de experiencia

  2008cited by this paper
• Predictive factors for early aspiration in liver abscess.

  2008cited by this paper
• The Vacuolar ATPase from Entamoeba histolytica: Molecular cloning of the gene encoding for the B subunit and subcellular localization of the protein

  2008cited by this paper
• Host innate immune receptors and beyond: making sense of microbial infections.

  2008cited by this paper
• Translating innate response into long‐lasting antibody response by the intrinsic antigen‐adjuvant properties of papaya mosaic virus

  2008cited by this paper
• [Amebic liver abscess. Three years experience].

  2008cited by this paper
• Role of TLRs/MyD88 in host resistance and pathogenesis during protozoan infection: lessons from malaria

  2008cited by this paper
• [Analysis of factors associated to failure of medical treatment of amoebic liver abscess].

  2008cited by this paper
• Lipopopeptidephosphoglycan from Entamoeba histolytica activates human macrophages and dendritic cells and reaches their late endosomes

  2007influential reference
• Structure and content of the Entamoeba histolytica genome.

  2007cited by this paper
• Activation of TLR2 and TLR4 by Glycosylphosphatidylinositols Derived from Toxoplasma gondii1

  2007cited by this paper
• Toll-Like Receptor 9-Dependent Macrophage Activation by Entamoeba histolytica DNA

  2007cited by this paper
• TLR-mediated cell signaling by malaria GPIs.

  2007cited by this paper
• Translating innate immunity into immunological memory: implications for vaccine development.

  2006cited by this paper
• Experimental amebiasis: a selected review of some in vivo models.

  2006cited by this paper
• Cytokine expression in CD4+ cells exposed to the monocyte locomotion inhibitory factor produced by Entamoeba histolytica

  2006cited by this paper
• Yellow fever vaccine YF-17D activates multiple dendritic cell subsets via TLR2, 7, 8, and 9 to stimulate polyvalent immunity

  2006cited by this paper
• New insights into molecular mechanisms of phagocytosis in Entamoeba histolytica by proteomic analysis.

  2006cited by this paper
• Toll-like receptor (TLR) polymorphisms in African children: common TLR-4 variants predispose to severe malaria.

  2006cited by this paper
• Salmonella porins induce a sustained, lifelong specific bactericidal antibody memory response

  2006cited by this paper
• Toll-like receptor recognition regulates immunodominance in an antimicrobial CD4+ T cell response.

  2006cited by this paper
• A bacterial carbohydrate links innate and adaptive responses through Toll-like receptor 2

  2006cited by this paper
• Protozoan encounters with Toll-like receptor signalling pathways: implications for host parasitism

  2006influential reference
• Cutting Edge: TLR9 and TLR2 Signaling Together Account for MyD88-Dependent Control of Parasitemia in Trypanosoma cruzi Infection1

  2006cited by this paper
• Induction of Proinflammatory Responses in Macrophages by the Glycosylphosphatidylinositols of Plasmodium falciparum

  2005cited by this paper
• The innate immune response to Entamoeba histolytica lipopeptidophosphoglycan is mediated by toll‐like receptors 2 and 4

  2005influential reference
• Toll-like receptor 9 mediates innate immune activation by the malaria pigment hemozoin

  2005cited by this paper
• LPS, TLR4 and infectious disease diversity

  2005influential reference
• Immunomodulation via Novel Use of TLR4 by the Filarial Nematode Phosphorylcholine-Containing Secreted Product, ES-621

  2005cited by this paper
• Manifold Mechanisms of Toll-Like Receptor-Ligand Recognition

  2005cited by this paper
• TLR11 Activation of Dendritic Cells by a Protozoan Profilin-Like Protein

  2005cited by this paper
• Reduction of Cell Surface Glycosylphosphatidylinositol Conjugates in Entamoeba histolytica by Antisense Blocking of E. histolytica GlcNAc-Phosphatidylinositol Deacetylase Expression: Effect on Cell Proliferation, Endocytosis, and Adhesion to Target Cells

  2005cited by this paper
• Entamoeba histolytica and Entamoeba dispar Utilize Externalized Phosphatidylserine for Recognition and Phagocytosis of Erythrocytes

  2005cited by this paper
• Exogenous and endogenous glycolipid antigens activate NKT cells during microbial infections

  2005cited by this paper
• Faculty Opinions recommendation of Induction of proinflammatory responses in macrophages by the glycosylphosphatidylinositols of Plasmodium falciparum: cell signaling receptors, glycosylphosphatidylinositol (GPI) structural requirement, and regulation of GPI activity.

  2005influential reference
• Innate Immunity: NKT Cells in the Spotlight

  2005cited by this paper
• Immunosuppressive treatment inhibits the development of amebic liver abscesses in hamsters.

  2004cited by this paper
• The EhADH112 recombinant polypeptide inhibits cell destruction and liver abscess formation by Entamoeba histolytica trophozoites

  2004cited by this paper
• Expression of Functional TLR4 Confers Proinflammatory Responsiveness to Trypanosoma cruzi Glycoinositolphospholipids and Higher Resistance to Infection with T. cruzi1

  2004cited by this paper
• Immunization with a tetramer derivative of an anti‐inflammatory pentapeptide produced by Entamoeba histolytica protects gerbils (Meriones unguiculatus) against experimental amoebic abscess of the liver

  2004cited by this paper
• Lysosomal Glycosphingolipid Recognition by NKT Cells

  2004cited by this paper
• Maturation of Dendritic Cell 2 Phenotype by a Helminth Glycan Uses a Toll-Like Receptor 4-Dependent Mechanism 1

  2003cited by this paper
• MyD88 is essential for clearance of Leishmania major: possible role for lipophosphoglycan and Toll‐like receptor 2 signaling

  2003cited by this paper
• Amoebiasis.

  2003cited by this paper
• Innate immune sensing and its roots: the story of endotoxin

  2003influential reference
• Leishmania lipophosphoglycan (LPG) activates NK cells through toll-like receptor-2.

  2003cited by this paper
• An anti‐inflammatory oligopeptide produced by Entamoeba histolytica down‐regulates the expression of pro‐inflammatory chemokines

  2003cited by this paper
• The Trypanosoma cruzi Tc52-Released Protein Induces Human Dendritic Cell Maturation, Signals Via Toll-Like Receptor 2, and Confers Protection Against Lethal Infection1

  2002cited by this paper
• Multidrug resistance in the protozoan parasite Entamoeba histolytica.

  2002cited by this paper
• A Monoclonal Antibody to the Amebic Lipophosphoglycan-Proteophosphoglycan Antigens Can Prevent Disease in Human Intestinal Xenografts Infected with Entamoeba histolytica

  2002cited by this paper
• A Novel Host-Parasite Lipid Cross-talk SCHISTOSOMAL LYSO-PHOSPHATIDYLSERINE ACTIVATES TOLL-LIKE RECEPTOR 2 AND AFFECTS IMMUNE POLARIZATION*

  2002cited by this paper
• A Novel Host-Parasite Lipid Cross-talk

  2002cited by this paper
• DNA from Protozoan Parasites Babesia bovis, Trypanosoma cruzi, and T. brucei Is Mitogenic for B Lymphocytes and Stimulates Macrophage Expression of Interleukin-12, Tumor Necrosis Factor Alpha, and Nitric Oxide

  2001cited by this paper
• Glycoconjugate structures of parasitic protozoa.

  2001cited by this paper
• A novel anti-inflammatory oligopeptide produced by Entamoeba histolytica.

  2001cited by this paper
• Activation of Toll-Like Receptor-2 by Glycosylphosphatidylinositol Anchors from a Protozoan Parasite1

  2001cited by this paper
• Development of Monoclonal Antibodies Which Specifically RecognizeEntamoeba histolytica in Preserved Stool Samples

  2001cited by this paper
• Pathogenesis of intestinal amebiasis: from molecules to disease.

  2000cited by this paper
• Lipophosphopeptidoglycan of Entamoeba histolytica induces an antiinflammatory innate immune response and downregulation of toll-like receptor 2 (TLR-2) gene expression in human monocytes.

  2000cited by this paper
• Entamoeba histolytica: a novel cysteine protease and an adhesin form the 112 kDa surface protein

  1999cited by this paper
• Selective ability of mouse CD1 to present glycolipids: alpha-galactosylceramide specifically stimulates V alpha 14+ NK T lymphocytes.

  1998cited by this paper
• Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in Tlr4 gene.

  1998influential reference
• Amoebiasis: new understanding and new goals.

  1998cited by this paper
• A family of human receptors structurally related to Drosophila Toll.

  1998cited by this paper
• Isolation and molecular characterization of a surface‐bound proteinase of Entamoeba histolytica

  1998cited by this paper
• Selective Ability of Mouse CD1 to Present Glycolipids: α-Galactosylceramide Specifically Stimulates Vα14+ NK T Lymphocytes

  1998cited by this paper
• Epithelial vs mesenchymal contribution to the extracellular matrix in the human intestine.

  1998cited by this paper
• A human homologue of the Drosophila Toll protein signals activation of adaptive immunity

  1997cited by this paper
• Virulent and avirulent Entamoeba histolytica and E. dispar differ in their cell surface phosphorylated glycolipids

  1997cited by this paper
• Protection against Invasive Amebiasis by a Single Monoclonal Antibody Directed against a Lipophosphoglycan Antigen Localized on the Surface of Entamoeba histolytica

  1997cited by this paper
• Cloning and expression of an Entamoeba histolytica NAPD+(-)dependent alcohol dehydrogenase gene.

  1996cited by this paper
• Short communication Cloning and characterization of the Entamoeba histolytica pyruvate: ferredoxin oxidoreductase gene'

  1996cited by this paper
• The dorsoventral regulatory gene cassette spätzle/Toll/cactus controls the potent antifungal response in Drosophila adults.

  1996cited by this paper
• Antigen induced eosinophilia protects gerbils (Meriones unguiculatus) against experimental amebic abscess of the liver.

  1995cited by this paper
• Purification and characterization of galactose- and N-acetylgalactosamine-specific adhesin lectin of Entamoeba histolytica.

  1995cited by this paper
• Cysteine endopeptidases of Entamoeba histolytica.

  1994cited by this paper
• [35] Cysteine endopeptidases of Entamoeba histolytica

  1994cited by this paper
• The structure, biosynthesis and function of glycosylated phosphatidylinositols in the parasitic protozoa and higher eukaryotes.

  1993cited by this paper
• Entamoeba histolytica trophozoites: a surface-associated cysteine protease.

  1993cited by this paper
• Isolation and partial characterization of a surface glycoconjugate of Entamoeba histolytica.

  1992cited by this paper
• The destruction of virulent Entamoeba histolytica by activated human eosinophils

  1992cited by this paper
• Encuesta serológica nacionalII Investigación de anticuerpos contra entamoeba histolytica en la república mexicana

  1992cited by this paper
• [Antibodies against Entamoeba histolytica in the Mexican Republic. 1974].

  1992cited by this paper
• Determination of taxonomic status of pathogenic and nonpathogenic Entamoeba histolytica zymodemes using isoenzyme analysis.

  1992cited by this paper

• Entamoeba histolytica: Plasma membrane components and virulence factors in the invasive process.

  2025cites this paper
• Toll-like receptor upregulation in liver and peripheral blood mononuclear cells of patients with amoebic liver abscess.

  2025cites this paper
• Malaria and an Amoebic Abscess in a Returning Traveler from Liberia

  2023cites this paper
• Apocynin, an NADPH Oxidase Enzyme Inhibitor, Prevents Amebic Liver Abscess in Hamster

  2023cites this paper
• Assessment of some immunological parameters for patients with diabetes mellitus infected with Entamoeba histolytica

  2022cites this paper
• AMELIORATING EFFECT OF CARICA PAPAYA L. FRUIT EXTRACT ON ENTAMOEBA HISTOLYTICA IN MICE

  2022cites this paper
• Entamoeba histolytica: Membrane and Non-Membrane Protein Structure, Function, Immune Response Interaction, and Vaccine Development

  2022cites this paper
• The gill transcriptome reveals unique antimicrobial features that protect Nibea albiflora from Cryptocaryon irritans infection.

  2021cites this paper
• Revisiting Drug Development Against the Neglected Tropical Disease, Amebiasis

  2021cites this paper
• Effects of Cytokines IFN-γ and TGF-β on the Functional Activity of Blood Mononuclear Cells against Giardia lamblia

  2021cites this paper
• The IL-33-ILC2 pathway protects from amebic colitis

  2021cites this paper
• The IL-33-ILC2 pathway protects from amebic colitis

  2021cites this paper
• “Immunoinformatic Identification of T-Cell and B-Cell Epitopes From Giardia lamblia Immunogenic Proteins as Candidates to Develop Peptide-Based Vaccines Against Giardiasis”

  2021cites this paper
• Androgens predispose males to monocyte-mediated immunopathology by inducing the expression of leukocyte recruitment factor CXCL1

  2020cites this paper
• Lipids in Entamoeba histolytica: Host-Dependence and Virulence Factors

  2020influential citation
• Immune Response to the Enteric Parasite Entamoeba histolytica.

  2020cites this paper
• IMMUNOLOGICAL IMPACT OF TOLL-LIKE RECEPTOR (TLR 2 AND TLR4) IN PATIENTS INFECTED WITH E. HISTOLYTICA

  2020cites this paper
• Non-vesicular Lipid Transport Machinery in Entamoeba histolytica

  2018cites this paper
• A review of the proposed role of neutrophils in rodent amebic liver abscess models

  2016cites this paper
• Entamoeba histolytica: influencia de la respuesta inmunitaria en la virulencia

  2016cites this paper
• ISSN 2320-5407 International Journal of Advanced Research (2016), Volume 4, Issue 2, 1212-1216

  2016influential citation
• Entamoeba histolytica induces human neutrophils to form NETs

  2016cites this paper
• TLR2 and TLR4 mediated host immune responses in major infectious diseases: a review

  2016cites this paper
• Characterization of surface phenotypic molecules of teleost dendritic cells.

  2015cites this paper
• Prevalent HLA Class II Alleles in Mexico City Appear to Confer Resistance to the Development of Amebic Liver Abscess

  2015cites this paper
• Molecular Basis of Pathogenesis in Amoebiasis

  2015cites this paper
• Effects of Crude Root Extracts of Senna Didymobotrya on Caecal Amoebiasis in Mice

  2015cites this paper
• Immune Response in Human Amebiasis: A Protective Response?

  2015cites this paper
• The Sympathetic Nervous System Regulates Inflammation in Amoebic Liver Abscess in Hamsters

  2015cites this paper
• Cell-Surface Molecules as Virulence Determinants in Entamoeba histolytica

  2015cites this paper
• CYTOTOXICITY AND IN VITRO ANTIAMOEBIC ACTIVITY OF SENNA DIDYMOBOTRYA CRUDE ROOT EXTRACTS IN COMPARISON WITH METRONIDAZOLE AGAINST ENTAMOEBA HISTOLYTICA

  2015cites this paper
• The Role of TLR2 in Infection and Immunity

  2012influential citation
• The role ofTLR2 in infection and immunity

  2012cites this paper
• Amebiasis cutánea: Conceptos actuales Cutaneous amebiasis: Current concepts

  2012influential citation
• Detection of Entamoeba histolytica in experimentally induced amoebic liver abscess: comparison of three staining methods.

  2012cites this paper
• Virulence and virulence factors in Entamoeba histolytica, the agent of human amoebiasis.

  2012cites this paper
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  2011cites this paper
• Comparison of Methods for Detection of Blastocystis Infection in Routinely Submitted Stool Samples, and also in IBS/IBD Patients in Ankara, Turkey

  2010cites this paper