Amplified Ribosomal DNA Restriction Analysis for the Preliminary Characterization of Native Diazotrophs Azotobacter, Acetobacter and Azospirillum

A 16S rRNA gene-based fingerprinting method was developed for the preliminary characterization of native diazotrophs bacterial isolates Azotobacter, Acetobacter and Azospirillum tested with reference strains belonging to the free-living nitrogen-fixing bacterial group. According to this method, the 16S rRNA gene was amplified using universal primers and PCR products were subsequently digested with TaqI, BamHI, HinfI and HaeIII. The analysis of the restriction profiles obtained showed that the method is able to define a unique species-specific phylotype (SSP) for each of the native diazotrophic bacterial isolates. Phylogenetic analysis of 16S rRNA indicated that these diazotrophic bacterial isolates belonged to respective genus Azotobacter, Acetobacter and Azospirillum and closely related to standard strains.

• Publication year

  2016

• Venue

  Journal of Pure and Applied Microbiology

• Publication date

  2016-09-30

• Fields of study

  Not labeled

• Identifiers
  DOI 10.22207/jpam.10.3.70
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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