Bradford Assay for Determining Protein Concentration.

C. Kielkopf,William J. Bauer,I. Urbatsch

Published 2020 in Cold Spring Harbor Protocols

ABSTRACT

The Bradford assay is a quick and fairly sensitive method for measuring the concentrations of proteins. It is based on the shift in absorbance maximum of Coomassie Brilliant Blue G-250 dye from 465 to 595 nm following binding to denatured proteins in solution.

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