Polarity and asymmetry in the arrangement of dynein and related structures in the Chlamydomonas axoneme

Cryoelectron tomography and subtomogram averaging reveal a high degree of structural asymmetry and polarization in dynein localization in the Chlamydomonas flagella.

• Publication year

  2012

• Venue

  Journal of Cell Biology

• Publication date

  2012-09-03

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1083/jcb.201201120PMID 22945936PMCID 3432765
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Comparative structural analysis of eukaryotic flagella and cilia from Chlamydomonas, Tetrahymena, and sea urchins.

  2012cited by this paper
• Mouse respiratory cilia with the asymmetric axonemal structure on sparsely distributed ciliary cells can generate overall directional flow.

  2012cited by this paper
• Cryoelectron tomography of radial spokes in cilia and flagella

  2011cited by this paper
• Discrete PIH proteins function in the cytoplasmic preassembly of different subsets of axonemal dyneins

  2010cited by this paper
• Nucleotide-induced global conformational changes of flagellar dynein arms revealed by in situ analysis

  2010cited by this paper
• Asymmetry of inner dynein arms and inter-doublet links in Chlamydomonas flagella

  2009cited by this paper
• The dynein regulatory complex is the nexin link and a major regulatory node in cilia and flagella

  2009influential reference
• Identification of dyneins that localize exclusively to the proximal portion of Chlamydomonas flagella

  2009cited by this paper
• An outer arm dynein light chain acts in a conformational switch for flagellar motility

  2009cited by this paper
• Twenty-five dyneins in Tetrahymena: A re-examination of the multidynein hypothesis.

  2008influential reference
• Molecular architecture of inner dynein arms in situ in Chlamydomonas reinhardtii flagella

  2008cited by this paper
• When cilia go bad: cilia defects and ciliopathies

  2008cited by this paper
• The architecture of outer dynein arms in situ.

  2007cited by this paper
• Novel 44-Kilodalton Subunit of Axonemal Dynein Conserved from Chlamydomonas to Mammals

  2007cited by this paper
• The Chlamydomonas Genome Reveals the Evolution of Key Animal and Plant Functions

  2007cited by this paper
• Dyneins Across Eukaryotes: A Comparative Genomic Analysis

  2007cited by this paper
• Analysis of cytoskeletal and motility proteins in the sea urchin genome assembly.

  2006cited by this paper
• A novel subunit of axonemal dynein conserved among lower and higher eukaryotes

  2006cited by this paper
• The Molecular Architecture of Axonemes Revealed by Cryoelectron Tomography

  2006cited by this paper
• TOM software toolbox: acquisition and analysis for electron tomography.

  2005cited by this paper
• Proteomic analysis of a eukaryotic cilium

  2005cited by this paper
• An Axonemal Dynein Particularly Important for Flagellar Movement at High Viscosity

  2005cited by this paper
• Mislocalization of DNAH5 and DNAH9 in respiratory cells from patients with primary ciliary dyskinesia.

  2005cited by this paper
• UCSF Chimera—A visualization system for exploratory research and analysis

  2004cited by this paper
• Bsoft: image and molecular processing in electron microscopy.

  2001influential reference
• Bsoft: Image and Molecular Processing in Electron Microscopy

  2001cited by this paper
• The 9 + 2 Axoneme Anchors Multiple Inner Arm Dyneins and a Network of Kinases and Phosphatases That Control Motility

  2000cited by this paper
• Highly divergent actin expressed in a Chlamydomonas mutant lacking the conventional actin gene.

  1998cited by this paper
• Dual-axis tomography: an approach with alignment methods that preserve resolution.

  1997cited by this paper
• The Chlamydomonas Dhc1 gene encodes a dynein heavy chain subunit required for assembly of the I1 inner arm complex.

  1997cited by this paper
• Computer visualization of three-dimensional image data using IMOD.

  1996cited by this paper
• SPIDER and WEB: processing and visualization of images in 3D electron microscopy and related fields.

  1996cited by this paper
• Flagellar assembly in two hundred and fifty easy-to-follow steps.

  1995cited by this paper
• Components of a "dynein regulatory complex" are located at the junction between the radial spokes and the dynein arms in Chlamydomonas flagella

  1994cited by this paper
• Mutations in the "dynein regulatory complex" alter the ATP-insensitive binding sites for inner arm dyneins in Chlamydomonas axonemes

  1994cited by this paper
• Translocation and rotation of microtubules caused by multiple species of Chlamydomonas inner-arm dynein

  1992cited by this paper
• The inner dynein arms I2 interact with a "dynein regulatory complex" in Chlamydomonas flagella

  1992cited by this paper
• Mutational analysis of centrin: an EF-hand protein associated with three distinct contractile fibers in the basal body apparatus of Chlamydomonas

  1992cited by this paper
• Arrangement of inner dynein arms in wild-type and mutant flagella of Chlamydomonas

  1992cited by this paper
• Two types of Chlamydomonas flagellar mutants missing different components of inner-arm dynein

  1991cited by this paper
• The proximal portion of Chlamydomonas flagella contains a distinct set of inner dynein arms

  1991cited by this paper
• Purification and characterization of Chlamydomonas flagellar dyneins.

  1986cited by this paper
• Isolation of Chlamydomonas flagella and flagellar axonemes.

  1986cited by this paper
• Purification and characterization of Chlamydomonas flagellar dyneins

  1986cited by this paper
• A nucleus-basal body connector in Chlamydomonas reinhardtii that may function in basal body localization or segregation

  1985cited by this paper
• Outer doublet heterogeneity reveals structural polarity related to beat direction in Chlamydomonas flagella

  1983influential reference
• Calcium control of waveform in isolated flagellar axonemes of chlamydomonas

  1980cited by this paper
• Isolated flagellar apparatus of Chlamydomonas: characterization of forward swimming and alteration of waveform and reversal of motion by calcium ions in vitro.

  1978cited by this paper
• Flagellar elongation and shortening in Chlamydomonas. III. structures attached to the tips of flagellar microtubules and their relationship to the directionality of flagellar microtubule assembly

  1977cited by this paper
• Adenosine triphosphate-induced sliding of tubules in trypsin-treated flagella of sea-urchin sperm.

  1971cited by this paper
• FLAGELLAR ELONGATION AND SHORTENING IN CHLAMYDOMONAS

  1970cited by this paper
• FLAGELLAR ELONGATION AND SHORTENING IN CHLAMYDOMONAS

  1969cited by this paper
• FLAGELLAR MOTION AND FINE STRUCTURE OF THE FLAGELLAR APPARATUS IN CHLAMYDOMONAS

  1967influential reference
• Cytochrome f and plastocyanin: their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardi.

  1965cited by this paper

• In situ structures of the ciliary tip show the multi-step mechanism of axoneme assembly

  2026cites this paper
• The structure of basal bodies and characterization of accessory components in Giardia intestinalis.

  2025cites this paper
• Restoration of the 3D structure of insect flight muscle from a rotationally averaged 2D X-ray diffraction pattern.

  2025cites this paper
• Asymmetries in the three-dimensional beat of Chlamydomonas reinhardtii flagella revealed by holographic microscopy.

  2025cites this paper
• The New Nexin-Dynein Regulatory Complex Component CCDC153 Is Dispensable for Ciliary Motility and Fertility in Mice.

  2025cites this paper
• Molecular Mechanisms Underlying the Construction of Ciliary Doublet Microtubules.

  2025cites this paper
• Chlamydomonas FBB18 is a ubiquitin-like protein essential for the cytoplasmic preassembly of various ciliary dyneins

  2025cites this paper
• Minimal design of a synthetic cilium

  2024cites this paper
• Assembly of FAP93 at the proximal axoneme in Chlamydomonas cilia

  2024cites this paper
• Functional and structural significance of the inner‐arm‐dynein subspecies d in ciliary motility

  2024influential citation
• Helical reconstruction of VP39 reveals principles for baculovirus nucleocapsid assembly

  2024cites this paper
• Multi-scale structures of the mammalian radial spoke and divergence of axonemal complexes in ependymal cilia

  2024cites this paper
• Swimming by Spinning: Spinning‐Top Type Rotations Regularize Sperm Swimming Into Persistently Progressive Paths in 3D

  2024cites this paper
• Chlamydomonas IC97, an intermediate chain of the flagellar dynein f/I1, is required for normal flagellar and cellular motility

  2024influential citation
• Proteins with proximal-distal asymmetries in axoneme localisation control flagellum beat frequency

  2024cites this paper
• The MBO2/FAP58 heterodimer stabilizes assembly of inner arm dynein b and reveals axoneme asymmetries involved in ciliary waveform

  2024influential citation
• Chlamydomonas dynein-preassembly-deficient mutants exhibit characteristic ciliary responses to viscous media

  2024cites this paper
• Structural determination and modeling of ciliary microtubules

  2024cites this paper
• DNALI1 interacts with the MEIG1/PACRG complex within the manchette and is required for proper sperm flagellum assembly in mice

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• The Small Interactor of PKD2 protein promotes the assembly and ciliary entry of the Chlamydomonas PKD2–mastigoneme complexes

  2023cites this paper
• In vivo imaging reveals independent intraflagellar transport of the nexin–dynein regulatory complex subunits DRC2 and DRC4

  2023cites this paper
• Spokes and barrels tune the asymmetric beating of mammalian sperm flagella

  2023cites this paper
• N-Terminal Processing and Modification of Ciliary Dyneins

  2023cites this paper
• The ciliary MBO2 complex targets assembly of inner arm dynein b and reveals additional doublet microtubule asymmetries

  2023influential citation
• CCDC176 stabilizes microtubule doublets 1 and 9 to ensure proper sperm movement.

  2023cites this paper
• Swimming by spinning: spinning-top type rotations regularize sperm swimming into persistently symmetric paths in 3D

  2023cites this paper
• Helical reconstruction of VP39 reveals principles for baculovirus nucleocapsid assembly

  2023cites this paper
• Small Interactor of PKD2 (SIP), a novel PKD2-related single-pass transmembrane protein, is required for proteolytic processing and ciliary import of Chlamydomonas PKD2

  2023cites this paper
• Integrated modeling of the Nexin-dynein regulatory complex reveals its regulatory mechanism

  2023cites this paper
• Axonemal structures reveal mechanoregulatory and disease mechanisms

  2023cites this paper
• ATP‐induced conformational change of axonemal outer dynein arms revealed by cryo‐electron tomography

  2023cites this paper
• ATP induced conformational change of axonemal outer dynein arms studied by cryo-electron tomography

  2022cites this paper
• Towards an atomic model of a beating ciliary axoneme.

  2022cites this paper
• The chiral beat of algal flagella: force and torque via imaging

  2022cites this paper
• Structure of Motile Cilia.

  2022cites this paper
• In situ architecture of the ciliary base reveals the stepwise assembly of intraflagellar transport trains

  2022cites this paper
• Ordered deployment of distinct ciliary beating machines in growing axonemes of vertebrate multiciliated cells

  2022influential citation
• Structural Biology of Cilia and Intraflagellar Transport.

  2022cites this paper
• In situ cryo-electron tomography reveals the asymmetric architecture of mammalian sperm axonemes

  2022cites this paper
• A look under the hood of the machine that makes cilia beat

  2022cites this paper
• A microtubule-dynein tethering complex regulates the axonemal A microtubule-dynein tethering complex regulates the axonemal inner dynein f (I1) inner dynein f (I1)

  2022cites this paper
• Ciliary beating patterns map onto a low-dimensional behavioural space

  2022cites this paper
• Cryo-EM structure of an active central apparatus

  2022cites this paper
• Structure of a microtubule-bound axonemal dynein

  2021cites this paper
• Cytoplasmic factories for axonemal dynein assembly.

  2021cites this paper
• Algal Ciliary Motility

  2021cites this paper
• Composition and function of ciliary inner‐dynein‐arm subunits studied in Chlamydomonas reinhardtii

  2021influential citation
• Ccdc113/Ccdc96 complex, a novel regulator of ciliary beating that connects radial spoke 3 to dynein g and the nexin link

  2021cites this paper
• A dynein-associated photoreceptor protein prevents ciliary acclimation to blue light

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• Electron microscopy for imaging organelles in plants and algae.

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• In situ architecture of the ciliary base reveals the stepwise assembly of IFT trains

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• Rapid multi-plane phase-contrast microscopy reveals torsional dynamics in flagellar motion.

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• Trypanosoma, Paramecium and Tetrahymena: From genomics to flagellar and ciliary structures and cytoskeleton dynamics.

  2020cites this paper
• Chlamydomonas PKD2 organizes mastigonemes, hair-like glycoprotein polymers on cilia

  2020cites this paper
• Cilia and centrosomes: Ultrastructural and mechanical perspectives.

  2020cites this paper
• Novel fibrillar structure in the inversin compartment of primary cilia revealed by 3D single-molecule superresolution microscopy

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• The single flagellum of Leishmania has a fixed polarisation of its asymmetric beat

  2020cites this paper
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  2020cites this paper
• A guided approach for subtomogram averaging of challenging macromolecular assemblies

  2020cites this paper
• TTC12 Loss-of-Function Mutations Cause Primary Ciliary Dyskinesia and Unveil Distinct Dynein Assembly Mechanisms in Motile Cilia Versus Flagella.

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  2020cites this paper
• Mutations in PIH proteins MOT48, TWI1 and PF13 define common and unique steps for preassembly of each, different ciliary dynein

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  2019cites this paper
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  2019influential citation
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  2019cites this paper
• Effect of curvature and normal forces on motor regulation of cilia

  2019influential citation
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  2019cites this paper
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  2019cites this paper
• Ciliary Proteins: Filling the Gaps. Recent Advances in Deciphering the Protein Composition of Motile Ciliary Complexes

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  2019influential citation
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  2019cites this paper
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  2019cites this paper
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  2018cites this paper
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